GFP-fluorescent protein on the study of blister spot in coffee plants

Detalhes bibliográficos
Autor(a) principal: Armesto, Cecilia
Data de Publicação: 2015
Outros Autores: Martins-Maia, Fernanda Gonçalves, Monteiro, Fernando Pereira, Abreu, Mário Sobral de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFLA
Texto Completo: http://repositorio.ufla.br/jspui/handle/1/29222
Resumo: In Brazil, Colletotrichum gloeosporioides is associated with a complex of symptoms in coffee culture. Although this pathogen had its pathogenesis observed and identified, its importance has still been questioned due to its several endophytic forms, raising doubts as to the real importance of the pathosystem. The aim of this study was to demonstrate, by using an isolate transformed with the gene gfp, the infection and colonization capability of C. gloeosporioides in coffee seedlings. After the fourth day of inoculation, manifestation of symptoms as punctual necrosis could be observed, which progressed during the evaluation period, culminating in the death of seedlings. Epifluorescence microscopy confirmed the presence of the pathogen in the seedlings, as well as the visualization of internal colonization of tissues, acervulus formation and conidium production, confirming that it was responsible for the observed symptoms.
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spelling GFP-fluorescent protein on the study of blister spot in coffee plantsA proteína fluorescente GFP no estudo da mancha manteigosa em plântulas de caféCafeeiro - Mancha manteigosaColletotrichum gloeosporioidesCoffee plants - Blister spotIn Brazil, Colletotrichum gloeosporioides is associated with a complex of symptoms in coffee culture. Although this pathogen had its pathogenesis observed and identified, its importance has still been questioned due to its several endophytic forms, raising doubts as to the real importance of the pathosystem. The aim of this study was to demonstrate, by using an isolate transformed with the gene gfp, the infection and colonization capability of C. gloeosporioides in coffee seedlings. After the fourth day of inoculation, manifestation of symptoms as punctual necrosis could be observed, which progressed during the evaluation period, culminating in the death of seedlings. Epifluorescence microscopy confirmed the presence of the pathogen in the seedlings, as well as the visualization of internal colonization of tissues, acervulus formation and conidium production, confirming that it was responsible for the observed symptoms.Colletotrichum gloeosporioides no Brasil está associado a um complexo de sintomas na cultura do café. Apesar da observação e da identificação da patogenicidade deste, o patógeno ainda tem sua importância questionada, devido às várias formas endofíticas do mesmo, levantando dúvidas sobre a real importância do patossistema. O presente trabalho teve como objetivo demonstrar por meio da utilização de um isolado transformado com o gene gfp a capacidade de infecção e colonização de C. gloeosporioides em plântulas de café. Pode-se observar após o quarto dia da inoculação a exteriorização de sintomas como necrose pontuais as quais progrediram durante o período de avaliação, culminando na morte das plântulas. Por meio de microscopia de epifluorescencia pode-se confirmar a presença do patógeno nas plântulas, assim como visualizar a colonização interna do tecido, a formação de acérvulos e produção de conídios, confirmando que o mesmo foi o responsável pelos sintomas observados.Grupo Paulista de Fitopatologia2018-05-15T16:37:00Z2018-05-15T16:37:00Z2015-04info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfARMESTO, C. et al. GFP-fluorescent protein on the study of blister spot in coffee plants. Summa Phytopathologica, Botucatu, v. 41, n. 2, p. 89-93, Apr./June 2015.http://repositorio.ufla.br/jspui/handle/1/29222Summa Phytopathologicareponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAhttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccessArmesto, CeciliaMartins-Maia, Fernanda GonçalvesMonteiro, Fernando PereiraAbreu, Mário Sobral deeng2023-05-26T19:51:14Zoai:localhost:1/29222Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2023-05-26T19:51:14Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false
dc.title.none.fl_str_mv GFP-fluorescent protein on the study of blister spot in coffee plants
A proteína fluorescente GFP no estudo da mancha manteigosa em plântulas de café
title GFP-fluorescent protein on the study of blister spot in coffee plants
spellingShingle GFP-fluorescent protein on the study of blister spot in coffee plants
Armesto, Cecilia
Cafeeiro - Mancha manteigosa
Colletotrichum gloeosporioides
Coffee plants - Blister spot
title_short GFP-fluorescent protein on the study of blister spot in coffee plants
title_full GFP-fluorescent protein on the study of blister spot in coffee plants
title_fullStr GFP-fluorescent protein on the study of blister spot in coffee plants
title_full_unstemmed GFP-fluorescent protein on the study of blister spot in coffee plants
title_sort GFP-fluorescent protein on the study of blister spot in coffee plants
author Armesto, Cecilia
author_facet Armesto, Cecilia
Martins-Maia, Fernanda Gonçalves
Monteiro, Fernando Pereira
Abreu, Mário Sobral de
author_role author
author2 Martins-Maia, Fernanda Gonçalves
Monteiro, Fernando Pereira
Abreu, Mário Sobral de
author2_role author
author
author
dc.contributor.author.fl_str_mv Armesto, Cecilia
Martins-Maia, Fernanda Gonçalves
Monteiro, Fernando Pereira
Abreu, Mário Sobral de
dc.subject.por.fl_str_mv Cafeeiro - Mancha manteigosa
Colletotrichum gloeosporioides
Coffee plants - Blister spot
topic Cafeeiro - Mancha manteigosa
Colletotrichum gloeosporioides
Coffee plants - Blister spot
description In Brazil, Colletotrichum gloeosporioides is associated with a complex of symptoms in coffee culture. Although this pathogen had its pathogenesis observed and identified, its importance has still been questioned due to its several endophytic forms, raising doubts as to the real importance of the pathosystem. The aim of this study was to demonstrate, by using an isolate transformed with the gene gfp, the infection and colonization capability of C. gloeosporioides in coffee seedlings. After the fourth day of inoculation, manifestation of symptoms as punctual necrosis could be observed, which progressed during the evaluation period, culminating in the death of seedlings. Epifluorescence microscopy confirmed the presence of the pathogen in the seedlings, as well as the visualization of internal colonization of tissues, acervulus formation and conidium production, confirming that it was responsible for the observed symptoms.
publishDate 2015
dc.date.none.fl_str_mv 2015-04
2018-05-15T16:37:00Z
2018-05-15T16:37:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv ARMESTO, C. et al. GFP-fluorescent protein on the study of blister spot in coffee plants. Summa Phytopathologica, Botucatu, v. 41, n. 2, p. 89-93, Apr./June 2015.
http://repositorio.ufla.br/jspui/handle/1/29222
identifier_str_mv ARMESTO, C. et al. GFP-fluorescent protein on the study of blister spot in coffee plants. Summa Phytopathologica, Botucatu, v. 41, n. 2, p. 89-93, Apr./June 2015.
url http://repositorio.ufla.br/jspui/handle/1/29222
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv http://creativecommons.org/licenses/by-nc/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Grupo Paulista de Fitopatologia
publisher.none.fl_str_mv Grupo Paulista de Fitopatologia
dc.source.none.fl_str_mv Summa Phytopathologica
reponame:Repositório Institucional da UFLA
instname:Universidade Federal de Lavras (UFLA)
instacron:UFLA
instname_str Universidade Federal de Lavras (UFLA)
instacron_str UFLA
institution UFLA
reponame_str Repositório Institucional da UFLA
collection Repositório Institucional da UFLA
repository.name.fl_str_mv Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)
repository.mail.fl_str_mv nivaldo@ufla.br || repositorio.biblioteca@ufla.br
_version_ 1815439257533153280

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