GFP-fluorescent protein on the study of blister spot in coffee plants

Detalhes bibliográficos
Autor(a) principal: Armesto,Cecilia
Data de Publicação: 2015
Outros Autores: Martins-Maia,Fernanda Gonçalves, Monteiro,Fernando Pereira, Abreu,Mário Sobral de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Summa phytopathologica (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-54052015000200089
Resumo: In Brazil, Colletotrichum gloeosporioides is associated with a complex of symptoms in coffee culture. Although this pathogen had its pathogenesis observed and identified, its importance has still been questioned due to its several endophytic forms, raising doubts as to the real importance of the pathosystem. The aim of this study was to demonstrate, by using an isolate transformed with the gene gfp, the infection and colonization capability of C. gloeosporioides in coffee seedlings. After the fourth day of inoculation, manifestation of symptoms as punctual necrosis could be observed, which progressed during the evaluation period, culminating in the death of seedlings. Epifluorescence microscopy confirmed the presence of the pathogen in the seedlings, as well as the visualization of internal colonization of tissues, acervulus formation and conidium production, confirming that it was responsible for the observed symptoms.
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spelling GFP-fluorescent protein on the study of blister spot in coffee plantsPathogenicityColletotrichumColonizationIn Brazil, Colletotrichum gloeosporioides is associated with a complex of symptoms in coffee culture. Although this pathogen had its pathogenesis observed and identified, its importance has still been questioned due to its several endophytic forms, raising doubts as to the real importance of the pathosystem. The aim of this study was to demonstrate, by using an isolate transformed with the gene gfp, the infection and colonization capability of C. gloeosporioides in coffee seedlings. After the fourth day of inoculation, manifestation of symptoms as punctual necrosis could be observed, which progressed during the evaluation period, culminating in the death of seedlings. Epifluorescence microscopy confirmed the presence of the pathogen in the seedlings, as well as the visualization of internal colonization of tissues, acervulus formation and conidium production, confirming that it was responsible for the observed symptoms.Grupo Paulista de Fitopatologia2015-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-54052015000200089Summa Phytopathologica v.41 n.2 2015reponame:Summa phytopathologica (Online)instname:Grupo Paulista de Fitopatologiainstacron:GPF10.1590/0100-5405/2047info:eu-repo/semantics/openAccessArmesto,CeciliaMartins-Maia,Fernanda GonçalvesMonteiro,Fernando PereiraAbreu,Mário Sobral deeng2015-06-29T00:00:00Zoai:scielo:S0100-54052015000200089Revistahttp://www.scielo.br/sphttps://old.scielo.br/oai/scielo-oai.phpsumma@fca.unesp.br1980-54540100-5405opendoar:2015-06-29T00:00Summa phytopathologica (Online) - Grupo Paulista de Fitopatologiafalse
dc.title.none.fl_str_mv GFP-fluorescent protein on the study of blister spot in coffee plants
title GFP-fluorescent protein on the study of blister spot in coffee plants
spellingShingle GFP-fluorescent protein on the study of blister spot in coffee plants
Armesto,Cecilia
Pathogenicity
Colletotrichum
Colonization
title_short GFP-fluorescent protein on the study of blister spot in coffee plants
title_full GFP-fluorescent protein on the study of blister spot in coffee plants
title_fullStr GFP-fluorescent protein on the study of blister spot in coffee plants
title_full_unstemmed GFP-fluorescent protein on the study of blister spot in coffee plants
title_sort GFP-fluorescent protein on the study of blister spot in coffee plants
author Armesto,Cecilia
author_facet Armesto,Cecilia
Martins-Maia,Fernanda Gonçalves
Monteiro,Fernando Pereira
Abreu,Mário Sobral de
author_role author
author2 Martins-Maia,Fernanda Gonçalves
Monteiro,Fernando Pereira
Abreu,Mário Sobral de
author2_role author
author
author
dc.contributor.author.fl_str_mv Armesto,Cecilia
Martins-Maia,Fernanda Gonçalves
Monteiro,Fernando Pereira
Abreu,Mário Sobral de
dc.subject.por.fl_str_mv Pathogenicity
Colletotrichum
Colonization
topic Pathogenicity
Colletotrichum
Colonization
description In Brazil, Colletotrichum gloeosporioides is associated with a complex of symptoms in coffee culture. Although this pathogen had its pathogenesis observed and identified, its importance has still been questioned due to its several endophytic forms, raising doubts as to the real importance of the pathosystem. The aim of this study was to demonstrate, by using an isolate transformed with the gene gfp, the infection and colonization capability of C. gloeosporioides in coffee seedlings. After the fourth day of inoculation, manifestation of symptoms as punctual necrosis could be observed, which progressed during the evaluation period, culminating in the death of seedlings. Epifluorescence microscopy confirmed the presence of the pathogen in the seedlings, as well as the visualization of internal colonization of tissues, acervulus formation and conidium production, confirming that it was responsible for the observed symptoms.
publishDate 2015
dc.date.none.fl_str_mv 2015-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-54052015000200089
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-54052015000200089
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/0100-5405/2047
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Grupo Paulista de Fitopatologia
publisher.none.fl_str_mv Grupo Paulista de Fitopatologia
dc.source.none.fl_str_mv Summa Phytopathologica v.41 n.2 2015
reponame:Summa phytopathologica (Online)
instname:Grupo Paulista de Fitopatologia
instacron:GPF
instname_str Grupo Paulista de Fitopatologia
instacron_str GPF
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reponame_str Summa phytopathologica (Online)
collection Summa phytopathologica (Online)
repository.name.fl_str_mv Summa phytopathologica (Online) - Grupo Paulista de Fitopatologia
repository.mail.fl_str_mv summa@fca.unesp.br
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