GFP-fluorescent protein on the study of blister spot in coffee plants
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Summa phytopathologica (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-54052015000200089 |
Resumo: | In Brazil, Colletotrichum gloeosporioides is associated with a complex of symptoms in coffee culture. Although this pathogen had its pathogenesis observed and identified, its importance has still been questioned due to its several endophytic forms, raising doubts as to the real importance of the pathosystem. The aim of this study was to demonstrate, by using an isolate transformed with the gene gfp, the infection and colonization capability of C. gloeosporioides in coffee seedlings. After the fourth day of inoculation, manifestation of symptoms as punctual necrosis could be observed, which progressed during the evaluation period, culminating in the death of seedlings. Epifluorescence microscopy confirmed the presence of the pathogen in the seedlings, as well as the visualization of internal colonization of tissues, acervulus formation and conidium production, confirming that it was responsible for the observed symptoms. |
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GFP-fluorescent protein on the study of blister spot in coffee plantsPathogenicityColletotrichumColonizationIn Brazil, Colletotrichum gloeosporioides is associated with a complex of symptoms in coffee culture. Although this pathogen had its pathogenesis observed and identified, its importance has still been questioned due to its several endophytic forms, raising doubts as to the real importance of the pathosystem. The aim of this study was to demonstrate, by using an isolate transformed with the gene gfp, the infection and colonization capability of C. gloeosporioides in coffee seedlings. After the fourth day of inoculation, manifestation of symptoms as punctual necrosis could be observed, which progressed during the evaluation period, culminating in the death of seedlings. Epifluorescence microscopy confirmed the presence of the pathogen in the seedlings, as well as the visualization of internal colonization of tissues, acervulus formation and conidium production, confirming that it was responsible for the observed symptoms.Grupo Paulista de Fitopatologia2015-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-54052015000200089Summa Phytopathologica v.41 n.2 2015reponame:Summa phytopathologica (Online)instname:Grupo Paulista de Fitopatologiainstacron:GPF10.1590/0100-5405/2047info:eu-repo/semantics/openAccessArmesto,CeciliaMartins-Maia,Fernanda GonçalvesMonteiro,Fernando PereiraAbreu,Mário Sobral deeng2015-06-29T00:00:00Zoai:scielo:S0100-54052015000200089Revistahttp://www.scielo.br/sphttps://old.scielo.br/oai/scielo-oai.phpsumma@fca.unesp.br1980-54540100-5405opendoar:2015-06-29T00:00Summa phytopathologica (Online) - Grupo Paulista de Fitopatologiafalse |
dc.title.none.fl_str_mv |
GFP-fluorescent protein on the study of blister spot in coffee plants |
title |
GFP-fluorescent protein on the study of blister spot in coffee plants |
spellingShingle |
GFP-fluorescent protein on the study of blister spot in coffee plants Armesto,Cecilia Pathogenicity Colletotrichum Colonization |
title_short |
GFP-fluorescent protein on the study of blister spot in coffee plants |
title_full |
GFP-fluorescent protein on the study of blister spot in coffee plants |
title_fullStr |
GFP-fluorescent protein on the study of blister spot in coffee plants |
title_full_unstemmed |
GFP-fluorescent protein on the study of blister spot in coffee plants |
title_sort |
GFP-fluorescent protein on the study of blister spot in coffee plants |
author |
Armesto,Cecilia |
author_facet |
Armesto,Cecilia Martins-Maia,Fernanda Gonçalves Monteiro,Fernando Pereira Abreu,Mário Sobral de |
author_role |
author |
author2 |
Martins-Maia,Fernanda Gonçalves Monteiro,Fernando Pereira Abreu,Mário Sobral de |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Armesto,Cecilia Martins-Maia,Fernanda Gonçalves Monteiro,Fernando Pereira Abreu,Mário Sobral de |
dc.subject.por.fl_str_mv |
Pathogenicity Colletotrichum Colonization |
topic |
Pathogenicity Colletotrichum Colonization |
description |
In Brazil, Colletotrichum gloeosporioides is associated with a complex of symptoms in coffee culture. Although this pathogen had its pathogenesis observed and identified, its importance has still been questioned due to its several endophytic forms, raising doubts as to the real importance of the pathosystem. The aim of this study was to demonstrate, by using an isolate transformed with the gene gfp, the infection and colonization capability of C. gloeosporioides in coffee seedlings. After the fourth day of inoculation, manifestation of symptoms as punctual necrosis could be observed, which progressed during the evaluation period, culminating in the death of seedlings. Epifluorescence microscopy confirmed the presence of the pathogen in the seedlings, as well as the visualization of internal colonization of tissues, acervulus formation and conidium production, confirming that it was responsible for the observed symptoms. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-54052015000200089 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-54052015000200089 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0100-5405/2047 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Grupo Paulista de Fitopatologia |
publisher.none.fl_str_mv |
Grupo Paulista de Fitopatologia |
dc.source.none.fl_str_mv |
Summa Phytopathologica v.41 n.2 2015 reponame:Summa phytopathologica (Online) instname:Grupo Paulista de Fitopatologia instacron:GPF |
instname_str |
Grupo Paulista de Fitopatologia |
instacron_str |
GPF |
institution |
GPF |
reponame_str |
Summa phytopathologica (Online) |
collection |
Summa phytopathologica (Online) |
repository.name.fl_str_mv |
Summa phytopathologica (Online) - Grupo Paulista de Fitopatologia |
repository.mail.fl_str_mv |
summa@fca.unesp.br |
_version_ |
1754193418052960256 |