Essential oil from Chenopodium ambrosioides L.: secretory structures, antibacterial and antioxidant activities

Detalhes bibliográficos
Autor(a) principal: Santiago, Juliana Andrade
Data de Publicação: 2016
Outros Autores: Cardoso, Maria das Graças, Batista, Luís Roberto, Castro, Evaristo Mauro de, Teixeira, Maria Luisa, Pires, Marinês Ferreira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFLA
Texto Completo: http://repositorio.ufla.br/jspui/handle/1/29935
Resumo: The aim of this study was to evaluate the antibacterial and antioxidant activities of essential oil from Chenopodium ambrosioides L. and to determine its secretory structures. The essential oil was extracted through the hydrodistillation technique using a modified Clevenger apparatus (2 hours) and chemically characterized by GC/MS and GC-FID. The antioxidant activity was determined by monitoring the reduction of 2,2-diphenyl-1-picrylhydrazyl (DPPH) and by the oxidation of the β-carotene/linoleic acid system. The evaluation of antibacterial activity was performed by the agar cavity diffusion technique using the microorganisms Staphylococcus aureus, Listeria monocytogenes, Escherichia coli and Salmonella Cholerasuis. The characterization of trichomes was accomplished by Scanning Electron Microscopy (SEM) and histochemical tests with Nadi and Sudan IV reagents. The antioxidant activity demonstrated by the β-carotene/acid linoleic test, with IC50 = 455.7 µg mL-1. This oil also presented antibacterial activity for both Gram-negative and Gram-positive bacteria. The minimal inhibitory concentration ranged 62.5 to 250 µL mL-1. The presence of terpenes in the glandular trichomes was observed, suggesting that the essential oil is secreted by these structures.
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spelling Essential oil from Chenopodium ambrosioides L.: secretory structures, antibacterial and antioxidant activitiesÓleo essencial de Chenopodium ambrosioides L.: estruturas secretoras, atividade antibacteriana e antioxidanteNatural productsAntioxidant assaysAntimicrobialTrichomesProdutos naturaisEnsaios antioxidantesAntimicrobianoTricomasThe aim of this study was to evaluate the antibacterial and antioxidant activities of essential oil from Chenopodium ambrosioides L. and to determine its secretory structures. The essential oil was extracted through the hydrodistillation technique using a modified Clevenger apparatus (2 hours) and chemically characterized by GC/MS and GC-FID. The antioxidant activity was determined by monitoring the reduction of 2,2-diphenyl-1-picrylhydrazyl (DPPH) and by the oxidation of the β-carotene/linoleic acid system. The evaluation of antibacterial activity was performed by the agar cavity diffusion technique using the microorganisms Staphylococcus aureus, Listeria monocytogenes, Escherichia coli and Salmonella Cholerasuis. The characterization of trichomes was accomplished by Scanning Electron Microscopy (SEM) and histochemical tests with Nadi and Sudan IV reagents. The antioxidant activity demonstrated by the β-carotene/acid linoleic test, with IC50 = 455.7 µg mL-1. This oil also presented antibacterial activity for both Gram-negative and Gram-positive bacteria. The minimal inhibitory concentration ranged 62.5 to 250 µL mL-1. The presence of terpenes in the glandular trichomes was observed, suggesting that the essential oil is secreted by these structures.Objetivou-se neste trabalho avaliar as atividades antibacteriana e antioxidante do óleo essencial de Chenopodium ambrosioides L. e determinar as suas es truturas secretoras. A extraç ão do óleo essencial foi realizada pelo método de hidrodestilação, utilizando o aparelho de Clevenger modificado com duração de 2 horas, sendo, posteriormente, analisado por CG/EM e CG-DIC. A atividade antioxidante foi determinada monitorando-se a redução do radical livre DPPH (2,2-d ifenil-1-picril-hidrazila) e utilizando o ensaio de oxidação do sistema β -caroteno/ácido linoleico. A avaliação da atividade antibacteriana foi realizada por meio da técnica difusão cavidade em ágar, utilizando os micro-organismos Staphylococcus aureus , Listeria monocytogenes , Escherichia coli e Salmonella Cholerasuis. A caracterização dos tricomas foi realizada por Microscopia Eletrônica de Varredura (MEV) e pelos testes histoquímicos com os reagentes de Nadi e Sudan IV. A atividade antioxidante foi evidenciada pelo teste β -caroteno/ácido linoleico, com CI 50 455,7 μ g mL -1 . O óleo também apresentou atividade antibacteriana tanto para bactérias Gram-negativas como para Gram-positivas. A concentraç ão mínima inibitória do mesmo variou entre 62,5 e 250 μ L mL -1 . Constatou- se a presença de compostos terpênic os nos tricomas glandulares, inferi ndo-se que o óleo essencial seja secretado nessas estruturas.Universidade Estadual de Maringá (UEM)2018-08-09T19:58:29Z2018-08-09T19:58:29Z2016-06info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfSANTIAGO, J. A. et al. Essential oil from Chenopodium ambrosioides L.: secretory structures, antibacterial and antioxidant activities. Acta Scientiarum. Biological Sciences, Maringá, v. 38, n. 2, p. 139-147, Apr./June 2016.http://repositorio.ufla.br/jspui/handle/1/29935Acta Scientiarum. Biological Sciencesreponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessSantiago, Juliana AndradeCardoso, Maria das GraçasBatista, Luís RobertoCastro, Evaristo Mauro deTeixeira, Maria LuisaPires, Marinês Ferreiraeng2018-08-09T19:58:29Zoai:localhost:1/29935Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2018-08-09T19:58:29Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false
dc.title.none.fl_str_mv Essential oil from Chenopodium ambrosioides L.: secretory structures, antibacterial and antioxidant activities
Óleo essencial de Chenopodium ambrosioides L.: estruturas secretoras, atividade antibacteriana e antioxidante
title Essential oil from Chenopodium ambrosioides L.: secretory structures, antibacterial and antioxidant activities
spellingShingle Essential oil from Chenopodium ambrosioides L.: secretory structures, antibacterial and antioxidant activities
Santiago, Juliana Andrade
Natural products
Antioxidant assays
Antimicrobial
Trichomes
Produtos naturais
Ensaios antioxidantes
Antimicrobiano
Tricomas
title_short Essential oil from Chenopodium ambrosioides L.: secretory structures, antibacterial and antioxidant activities
title_full Essential oil from Chenopodium ambrosioides L.: secretory structures, antibacterial and antioxidant activities
title_fullStr Essential oil from Chenopodium ambrosioides L.: secretory structures, antibacterial and antioxidant activities
title_full_unstemmed Essential oil from Chenopodium ambrosioides L.: secretory structures, antibacterial and antioxidant activities
title_sort Essential oil from Chenopodium ambrosioides L.: secretory structures, antibacterial and antioxidant activities
author Santiago, Juliana Andrade
author_facet Santiago, Juliana Andrade
Cardoso, Maria das Graças
Batista, Luís Roberto
Castro, Evaristo Mauro de
Teixeira, Maria Luisa
Pires, Marinês Ferreira
author_role author
author2 Cardoso, Maria das Graças
Batista, Luís Roberto
Castro, Evaristo Mauro de
Teixeira, Maria Luisa
Pires, Marinês Ferreira
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Santiago, Juliana Andrade
Cardoso, Maria das Graças
Batista, Luís Roberto
Castro, Evaristo Mauro de
Teixeira, Maria Luisa
Pires, Marinês Ferreira
dc.subject.por.fl_str_mv Natural products
Antioxidant assays
Antimicrobial
Trichomes
Produtos naturais
Ensaios antioxidantes
Antimicrobiano
Tricomas
topic Natural products
Antioxidant assays
Antimicrobial
Trichomes
Produtos naturais
Ensaios antioxidantes
Antimicrobiano
Tricomas
description The aim of this study was to evaluate the antibacterial and antioxidant activities of essential oil from Chenopodium ambrosioides L. and to determine its secretory structures. The essential oil was extracted through the hydrodistillation technique using a modified Clevenger apparatus (2 hours) and chemically characterized by GC/MS and GC-FID. The antioxidant activity was determined by monitoring the reduction of 2,2-diphenyl-1-picrylhydrazyl (DPPH) and by the oxidation of the β-carotene/linoleic acid system. The evaluation of antibacterial activity was performed by the agar cavity diffusion technique using the microorganisms Staphylococcus aureus, Listeria monocytogenes, Escherichia coli and Salmonella Cholerasuis. The characterization of trichomes was accomplished by Scanning Electron Microscopy (SEM) and histochemical tests with Nadi and Sudan IV reagents. The antioxidant activity demonstrated by the β-carotene/acid linoleic test, with IC50 = 455.7 µg mL-1. This oil also presented antibacterial activity for both Gram-negative and Gram-positive bacteria. The minimal inhibitory concentration ranged 62.5 to 250 µL mL-1. The presence of terpenes in the glandular trichomes was observed, suggesting that the essential oil is secreted by these structures.
publishDate 2016
dc.date.none.fl_str_mv 2016-06
2018-08-09T19:58:29Z
2018-08-09T19:58:29Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv SANTIAGO, J. A. et al. Essential oil from Chenopodium ambrosioides L.: secretory structures, antibacterial and antioxidant activities. Acta Scientiarum. Biological Sciences, Maringá, v. 38, n. 2, p. 139-147, Apr./June 2016.
http://repositorio.ufla.br/jspui/handle/1/29935
identifier_str_mv SANTIAGO, J. A. et al. Essential oil from Chenopodium ambrosioides L.: secretory structures, antibacterial and antioxidant activities. Acta Scientiarum. Biological Sciences, Maringá, v. 38, n. 2, p. 139-147, Apr./June 2016.
url http://repositorio.ufla.br/jspui/handle/1/29935
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Estadual de Maringá (UEM)
publisher.none.fl_str_mv Universidade Estadual de Maringá (UEM)
dc.source.none.fl_str_mv Acta Scientiarum. Biological Sciences
reponame:Repositório Institucional da UFLA
instname:Universidade Federal de Lavras (UFLA)
instacron:UFLA
instname_str Universidade Federal de Lavras (UFLA)
instacron_str UFLA
institution UFLA
reponame_str Repositório Institucional da UFLA
collection Repositório Institucional da UFLA
repository.name.fl_str_mv Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)
repository.mail.fl_str_mv nivaldo@ufla.br || repositorio.biblioteca@ufla.br
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