Effects of protein level and methionine supplementation during the periparturient period of dairy cows

Detalhes bibliográficos
Autor(a) principal: Cardoso, Fabiana de Freitas
Data de Publicação: 2018
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositório Institucional da UFLA
Texto Completo: http://repositorio.ufla.br/jspui/handle/1/29011
Resumo: Cows go through a significant negative protein balance during the first 30 days of lactation. Given the functional effects of amino acids (AA) on health and inflammation, especially in challenging periods such as around calving, higher levels of protein and specific AA may improve health and intake. The response of dairy cows to three protein supplementation strategies during the transition period and through the first 45 days in milk was evaluated. Forty-six cows were blocked based on parity (primiparous vs. multiparous) and date of calving and within each block were randomly assigned to one of three treatments: low protein (LP), high protein (HP) or high protein with inclusion of rumen protected methionine (HPM). Treatments were offered from day -21 to 45 days relative to calving. Pre- and post-partum diets were formulated to contain high metabolizable protein (MP) capacity, and HP and HPM presented higher MP balance compared to LP treatment. Thirty-nine cows completed the experiment. Cows fed HPM consumed 2.8 kg/d more DM than did cows on LP treatment during pre- partum. We found no differences on BW and BCS across treatments in pre- and post- partum. Cows fed HP produced 2.5 kg/d more milk during the first 45 days of lactation than cows fed LP, although it was not statistically significant. Addition of RPMet to the HP diet did not increase milk production. Greater protein supplementation (HPM) reduced milk/DMI, increased milk urea nitrogen, and tended to increase plasma urea nitrogen. There was a lower blood interleukin (IL) 1 concentration on the cows fed HPM compared to the diet LP.The lymphocyte concentration in the blood was higher in the treatment of low protein. However, blood concentrations of insulin, glucose, non-esterified fatty acid, creatinine, beta-hydroxybutyrate, calcium, IL10 and TNFα did not differ among treatments during the experimental period.
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spelling Effects of protein level and methionine supplementation during the periparturient period of dairy cowsMetioninaVacas leiteiras - NutriçãoProteína do leiteVacas leiteiras - Sistema imuneMethionineDairy Cows - NutritionMilk proteinDairy Cows - Immune systemZootecniaCows go through a significant negative protein balance during the first 30 days of lactation. Given the functional effects of amino acids (AA) on health and inflammation, especially in challenging periods such as around calving, higher levels of protein and specific AA may improve health and intake. The response of dairy cows to three protein supplementation strategies during the transition period and through the first 45 days in milk was evaluated. Forty-six cows were blocked based on parity (primiparous vs. multiparous) and date of calving and within each block were randomly assigned to one of three treatments: low protein (LP), high protein (HP) or high protein with inclusion of rumen protected methionine (HPM). Treatments were offered from day -21 to 45 days relative to calving. Pre- and post-partum diets were formulated to contain high metabolizable protein (MP) capacity, and HP and HPM presented higher MP balance compared to LP treatment. Thirty-nine cows completed the experiment. Cows fed HPM consumed 2.8 kg/d more DM than did cows on LP treatment during pre- partum. We found no differences on BW and BCS across treatments in pre- and post- partum. Cows fed HP produced 2.5 kg/d more milk during the first 45 days of lactation than cows fed LP, although it was not statistically significant. Addition of RPMet to the HP diet did not increase milk production. Greater protein supplementation (HPM) reduced milk/DMI, increased milk urea nitrogen, and tended to increase plasma urea nitrogen. There was a lower blood interleukin (IL) 1 concentration on the cows fed HPM compared to the diet LP.The lymphocyte concentration in the blood was higher in the treatment of low protein. However, blood concentrations of insulin, glucose, non-esterified fatty acid, creatinine, beta-hydroxybutyrate, calcium, IL10 and TNFα did not differ among treatments during the experimental period.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)O início da lactação de vacas leiteiras é caracterizado por um balanço negativo de proteína e energia. Além da alta exigência nessa fase para o início da produção de leite, o consumo de matéria seca é baixo. Com isso, são necessárias estratégias de alimentação para aliviar o balanço negativo de nutrientes nesta fase. Em períodos de estresse, como em torno do parto, pode ocorrer imunodepressão, com a possibilidade de comprometer a capacidade das vacas em lidar com os desafios, tornando-as mais suscetíveis a doenças. O objetivo deste estudo foi examinar o efeito do aumento do aporte de proteína metabolizável e o ajuste no perfil de aminoácidos dessa proteína para vacas durante o período de pré-parto e início da lactação. Ao explorar este objetivo, examinamos o efeito de aumentar o nível de proteína da dieta com ou sem metionina protegida da degradação ruminal sobre o desempenho e a saúde das vacas leiteiras durante o período de transição e nos primeiros 45 dias de lactação. Quarenta e seis vacas foram blocadas com base na paridade (primíparas vs multíparas) e data de parto e dentro de cada bloco foram distribuídas aleatoriamente para um dos três tratamentos: baixa proteína (LP), alta proteína (HP) ou alta proteína com inclusão de metionina protegida no rúmen (HPM). Os tratamentos foram oferecidos do dia -21 a 45 dias em relação ao parto. Trinta e nove vacas completaram o experimento. As vacas alimentadas com HPM consumiram 2,8 kg/d mais matéria seca do que as vacas no tratamento de LP durante o pré-parto. Não encontramos diferenças no peso corporal e no escore de condição corporal entre os tratamentos no pré e pós-parto. As vacas alimentadas com HP produziram 2,5 kg/d mais leite durante os primeiros 45 dias de lactação do que as vacas alimentadas com LP, embora essa diferença não tenha sido estatisticamente significante. A adição de metionina à dieta HP não aumentou a produção de leite. Maior suplementação protéica (HP) em relação a níveis mais baixos de proteína na dieta (LP) reduziu a relação leite/ingestão de matéria seca, aumentou o nitrogênio ureico no leite e tendeu a aumentar o nitrogênio ureico no plasma. Vacas alimentadas com HPM apresentaram menor concentração plasmática de interleucina (IL) 1 em relação as vacas no tratamento LP. A concentração sangúinea de linfócitos foi maior no tratamento de baixa proteína em relação aos tratamentos de alta proteína ( HP e HPM). No entanto, as concentrações plasmáticas de glicose, creatinina, cálcio, IL10 e TNFα e as concentrações séricas de insulina, ácidos graxos não esterificados e beta-hidroxibutirato, não diferiram entre os tratamentos durante o período experimental.Universidade Federal de LavrasPrograma de Pós-Graduação em ZootecniaUFLAbrasilDepartamento de ZootecniaDanes, Marina de Arruda CamargoPereira, Marcos NevesDonkin, ShawnPeconick, Ana PaulaCardoso, Fabiana de Freitas2018-04-11T16:28:04Z2018-04-11T16:28:04Z2018-04-102018-02-06info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfCARDOSO, F. de F. Effects of protein level and methionine supplementation during the periparturient period of dairy cows. 2018. 56 p. Dissertação (Mestrado em Zootecnia)-Universidade Federal de Lavras, Lavras, 2018.http://repositorio.ufla.br/jspui/handle/1/29011enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLA2019-04-24T12:46:24Zoai:localhost:1/29011Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2019-04-24T12:46:24Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false
dc.title.none.fl_str_mv Effects of protein level and methionine supplementation during the periparturient period of dairy cows
title Effects of protein level and methionine supplementation during the periparturient period of dairy cows
spellingShingle Effects of protein level and methionine supplementation during the periparturient period of dairy cows
Cardoso, Fabiana de Freitas
Metionina
Vacas leiteiras - Nutrição
Proteína do leite
Vacas leiteiras - Sistema imune
Methionine
Dairy Cows - Nutrition
Milk protein
Dairy Cows - Immune system
Zootecnia
title_short Effects of protein level and methionine supplementation during the periparturient period of dairy cows
title_full Effects of protein level and methionine supplementation during the periparturient period of dairy cows
title_fullStr Effects of protein level and methionine supplementation during the periparturient period of dairy cows
title_full_unstemmed Effects of protein level and methionine supplementation during the periparturient period of dairy cows
title_sort Effects of protein level and methionine supplementation during the periparturient period of dairy cows
author Cardoso, Fabiana de Freitas
author_facet Cardoso, Fabiana de Freitas
author_role author
dc.contributor.none.fl_str_mv Danes, Marina de Arruda Camargo
Pereira, Marcos Neves
Donkin, Shawn
Peconick, Ana Paula
dc.contributor.author.fl_str_mv Cardoso, Fabiana de Freitas
dc.subject.por.fl_str_mv Metionina
Vacas leiteiras - Nutrição
Proteína do leite
Vacas leiteiras - Sistema imune
Methionine
Dairy Cows - Nutrition
Milk protein
Dairy Cows - Immune system
Zootecnia
topic Metionina
Vacas leiteiras - Nutrição
Proteína do leite
Vacas leiteiras - Sistema imune
Methionine
Dairy Cows - Nutrition
Milk protein
Dairy Cows - Immune system
Zootecnia
description Cows go through a significant negative protein balance during the first 30 days of lactation. Given the functional effects of amino acids (AA) on health and inflammation, especially in challenging periods such as around calving, higher levels of protein and specific AA may improve health and intake. The response of dairy cows to three protein supplementation strategies during the transition period and through the first 45 days in milk was evaluated. Forty-six cows were blocked based on parity (primiparous vs. multiparous) and date of calving and within each block were randomly assigned to one of three treatments: low protein (LP), high protein (HP) or high protein with inclusion of rumen protected methionine (HPM). Treatments were offered from day -21 to 45 days relative to calving. Pre- and post-partum diets were formulated to contain high metabolizable protein (MP) capacity, and HP and HPM presented higher MP balance compared to LP treatment. Thirty-nine cows completed the experiment. Cows fed HPM consumed 2.8 kg/d more DM than did cows on LP treatment during pre- partum. We found no differences on BW and BCS across treatments in pre- and post- partum. Cows fed HP produced 2.5 kg/d more milk during the first 45 days of lactation than cows fed LP, although it was not statistically significant. Addition of RPMet to the HP diet did not increase milk production. Greater protein supplementation (HPM) reduced milk/DMI, increased milk urea nitrogen, and tended to increase plasma urea nitrogen. There was a lower blood interleukin (IL) 1 concentration on the cows fed HPM compared to the diet LP.The lymphocyte concentration in the blood was higher in the treatment of low protein. However, blood concentrations of insulin, glucose, non-esterified fatty acid, creatinine, beta-hydroxybutyrate, calcium, IL10 and TNFα did not differ among treatments during the experimental period.
publishDate 2018
dc.date.none.fl_str_mv 2018-04-11T16:28:04Z
2018-04-11T16:28:04Z
2018-04-10
2018-02-06
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv CARDOSO, F. de F. Effects of protein level and methionine supplementation during the periparturient period of dairy cows. 2018. 56 p. Dissertação (Mestrado em Zootecnia)-Universidade Federal de Lavras, Lavras, 2018.
http://repositorio.ufla.br/jspui/handle/1/29011
identifier_str_mv CARDOSO, F. de F. Effects of protein level and methionine supplementation during the periparturient period of dairy cows. 2018. 56 p. Dissertação (Mestrado em Zootecnia)-Universidade Federal de Lavras, Lavras, 2018.
url http://repositorio.ufla.br/jspui/handle/1/29011
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Lavras
Programa de Pós-Graduação em Zootecnia
UFLA
brasil
Departamento de Zootecnia
publisher.none.fl_str_mv Universidade Federal de Lavras
Programa de Pós-Graduação em Zootecnia
UFLA
brasil
Departamento de Zootecnia
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFLA
instname:Universidade Federal de Lavras (UFLA)
instacron:UFLA
instname_str Universidade Federal de Lavras (UFLA)
instacron_str UFLA
institution UFLA
reponame_str Repositório Institucional da UFLA
collection Repositório Institucional da UFLA
repository.name.fl_str_mv Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)
repository.mail.fl_str_mv nivaldo@ufla.br || repositorio.biblioteca@ufla.br
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