Isoflavone and protein content in soybeans grains submitted to flooding at different stages of development

Detalhes bibliográficos
Autor(a) principal: Fante, Camila Argenta
Data de Publicação: 2011
Outros Autores: Goulart, Patrícia de Fátima Pereira, Alves, José Donizeti, Henrique, Paôla de Castro, Fries, Daniela Deitos
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFLA
Texto Completo: http://repositorio.ufla.br/jspui/handle/1/38511
Resumo: The stress imposed on plants by soil flooding constitutes a major barrier to growth and productivity. The identification of soybean varieties that produce higher levels of isoflavones, is necessary as soybeans have been used as human food to reduce risks of chronic diseases. Thus, this study was conducted with the objective of quantifying proteins and isoflavones in soybean cultivars subjected to flooding at various stages of development. The cultivars 'BRS267', 'BRS257' and 'BRS213' were subjected to 15 days of flooding, starting at the stages V6 and V8 and 11 days under stress starting at the stage R4. The proteins in the grain were extracted and quantified and analyzed by SDS-PAGE electrophoresis. Isoflavones were extracted, separated and quantified on HPLC. The electrophoretic analysis of the three cultivars under study revealed the same pattern of banding relative to the total protein regardless of the treatment. However, it was noted that flooding led to an increase in the total contents of isoflavones in the BRS 267 plants flooded in stage R4, remaining constant in other cultivars.
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spelling Isoflavone and protein content in soybeans grains submitted to flooding at different stages of developmentTeor de isoflavonas e proteínas nos grãos de soja submetida ao alagamento em diferentes estádios de desenvolvimentoGlycine maxHypoxiaFunctional foodHipoxiaAlimento funcionalThe stress imposed on plants by soil flooding constitutes a major barrier to growth and productivity. The identification of soybean varieties that produce higher levels of isoflavones, is necessary as soybeans have been used as human food to reduce risks of chronic diseases. Thus, this study was conducted with the objective of quantifying proteins and isoflavones in soybean cultivars subjected to flooding at various stages of development. The cultivars 'BRS267', 'BRS257' and 'BRS213' were subjected to 15 days of flooding, starting at the stages V6 and V8 and 11 days under stress starting at the stage R4. The proteins in the grain were extracted and quantified and analyzed by SDS-PAGE electrophoresis. Isoflavones were extracted, separated and quantified on HPLC. The electrophoretic analysis of the three cultivars under study revealed the same pattern of banding relative to the total protein regardless of the treatment. However, it was noted that flooding led to an increase in the total contents of isoflavones in the BRS 267 plants flooded in stage R4, remaining constant in other cultivars.O estresse imposto às plantas pelo alagamento do solo constitui uma barreira para o crescimento e produtividade destas. A identificação de cultivares de soja que possam produzir maior teor de isoflavonas torna-se necessária à medida que a soja vem sendo utilizada na alimentação humana e para redução dos riscos de doenças crônicas. Dessa forma, o presente estudo foi realizado com o objetivo de quantificar isoflavonas e proteínas em cultivares de soja submetidas ao alagamento em diferentes estádios de desenvolvimento. As cultivares 'BRS 267', 'BRS 257' e 'BRS 213' foram submetidas a 15 dias de alagamento, nos estádios V6 e V8 e 11 dias sob estresse, no estádio R4. As proteínas no grão foram extraídas e quantificadas e analisadas as eletroforeses em SDS-PAGE. Os isoflavonóides foram extraídos, separados e quantificados por HPLC. A análise por eletroforese das três cultivares revelou o mesmo padrão de bandas relativas às proteínas totais, independente do tratamento utilizado. O alagamento promoveu aumento no teor total de isoflavonas nas plantas alagadas no estádio R4 da cultivar 'BRS 267', permanecendo constante nas demais cultivares.Universidade Federal de Santa Maria2020-01-15T11:45:57Z2020-01-15T11:45:57Z2011-12info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfFANTE, C. A. et al. Isoflavone and protein content in soybeans grains submitted to flooding at different stages of development. Ciência Rural, Santa Maria, v. 41, n. 12, p. 2224-2229, Dec. 2011.http://repositorio.ufla.br/jspui/handle/1/38511Ciência Ruralreponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAAttribution-NonCommercial 4.0 Internationalhttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccessFante, Camila ArgentaGoulart, Patrícia de Fátima PereiraAlves, José DonizetiHenrique, Paôla de CastroFries, Daniela Deitoseng2020-01-15T11:45:58Zoai:localhost:1/38511Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2020-01-15T11:45:58Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false
dc.title.none.fl_str_mv Isoflavone and protein content in soybeans grains submitted to flooding at different stages of development
Teor de isoflavonas e proteínas nos grãos de soja submetida ao alagamento em diferentes estádios de desenvolvimento
title Isoflavone and protein content in soybeans grains submitted to flooding at different stages of development
spellingShingle Isoflavone and protein content in soybeans grains submitted to flooding at different stages of development
Fante, Camila Argenta
Glycine max
Hypoxia
Functional food
Hipoxia
Alimento funcional
title_short Isoflavone and protein content in soybeans grains submitted to flooding at different stages of development
title_full Isoflavone and protein content in soybeans grains submitted to flooding at different stages of development
title_fullStr Isoflavone and protein content in soybeans grains submitted to flooding at different stages of development
title_full_unstemmed Isoflavone and protein content in soybeans grains submitted to flooding at different stages of development
title_sort Isoflavone and protein content in soybeans grains submitted to flooding at different stages of development
author Fante, Camila Argenta
author_facet Fante, Camila Argenta
Goulart, Patrícia de Fátima Pereira
Alves, José Donizeti
Henrique, Paôla de Castro
Fries, Daniela Deitos
author_role author
author2 Goulart, Patrícia de Fátima Pereira
Alves, José Donizeti
Henrique, Paôla de Castro
Fries, Daniela Deitos
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Fante, Camila Argenta
Goulart, Patrícia de Fátima Pereira
Alves, José Donizeti
Henrique, Paôla de Castro
Fries, Daniela Deitos
dc.subject.por.fl_str_mv Glycine max
Hypoxia
Functional food
Hipoxia
Alimento funcional
topic Glycine max
Hypoxia
Functional food
Hipoxia
Alimento funcional
description The stress imposed on plants by soil flooding constitutes a major barrier to growth and productivity. The identification of soybean varieties that produce higher levels of isoflavones, is necessary as soybeans have been used as human food to reduce risks of chronic diseases. Thus, this study was conducted with the objective of quantifying proteins and isoflavones in soybean cultivars subjected to flooding at various stages of development. The cultivars 'BRS267', 'BRS257' and 'BRS213' were subjected to 15 days of flooding, starting at the stages V6 and V8 and 11 days under stress starting at the stage R4. The proteins in the grain were extracted and quantified and analyzed by SDS-PAGE electrophoresis. Isoflavones were extracted, separated and quantified on HPLC. The electrophoretic analysis of the three cultivars under study revealed the same pattern of banding relative to the total protein regardless of the treatment. However, it was noted that flooding led to an increase in the total contents of isoflavones in the BRS 267 plants flooded in stage R4, remaining constant in other cultivars.
publishDate 2011
dc.date.none.fl_str_mv 2011-12
2020-01-15T11:45:57Z
2020-01-15T11:45:57Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv FANTE, C. A. et al. Isoflavone and protein content in soybeans grains submitted to flooding at different stages of development. Ciência Rural, Santa Maria, v. 41, n. 12, p. 2224-2229, Dec. 2011.
http://repositorio.ufla.br/jspui/handle/1/38511
identifier_str_mv FANTE, C. A. et al. Isoflavone and protein content in soybeans grains submitted to flooding at different stages of development. Ciência Rural, Santa Maria, v. 41, n. 12, p. 2224-2229, Dec. 2011.
url http://repositorio.ufla.br/jspui/handle/1/38511
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv Attribution-NonCommercial 4.0 International
http://creativecommons.org/licenses/by-nc/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NonCommercial 4.0 International
http://creativecommons.org/licenses/by-nc/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Santa Maria
publisher.none.fl_str_mv Universidade Federal de Santa Maria
dc.source.none.fl_str_mv Ciência Rural
reponame:Repositório Institucional da UFLA
instname:Universidade Federal de Lavras (UFLA)
instacron:UFLA
instname_str Universidade Federal de Lavras (UFLA)
instacron_str UFLA
institution UFLA
reponame_str Repositório Institucional da UFLA
collection Repositório Institucional da UFLA
repository.name.fl_str_mv Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)
repository.mail.fl_str_mv nivaldo@ufla.br || repositorio.biblioteca@ufla.br
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