Isoflavone and protein content in soybeans grains submitted to flooding at different stages of development
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFLA |
Texto Completo: | http://repositorio.ufla.br/jspui/handle/1/38511 |
Resumo: | The stress imposed on plants by soil flooding constitutes a major barrier to growth and productivity. The identification of soybean varieties that produce higher levels of isoflavones, is necessary as soybeans have been used as human food to reduce risks of chronic diseases. Thus, this study was conducted with the objective of quantifying proteins and isoflavones in soybean cultivars subjected to flooding at various stages of development. The cultivars 'BRS267', 'BRS257' and 'BRS213' were subjected to 15 days of flooding, starting at the stages V6 and V8 and 11 days under stress starting at the stage R4. The proteins in the grain were extracted and quantified and analyzed by SDS-PAGE electrophoresis. Isoflavones were extracted, separated and quantified on HPLC. The electrophoretic analysis of the three cultivars under study revealed the same pattern of banding relative to the total protein regardless of the treatment. However, it was noted that flooding led to an increase in the total contents of isoflavones in the BRS 267 plants flooded in stage R4, remaining constant in other cultivars. |
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Isoflavone and protein content in soybeans grains submitted to flooding at different stages of developmentTeor de isoflavonas e proteínas nos grãos de soja submetida ao alagamento em diferentes estádios de desenvolvimentoGlycine maxHypoxiaFunctional foodHipoxiaAlimento funcionalThe stress imposed on plants by soil flooding constitutes a major barrier to growth and productivity. The identification of soybean varieties that produce higher levels of isoflavones, is necessary as soybeans have been used as human food to reduce risks of chronic diseases. Thus, this study was conducted with the objective of quantifying proteins and isoflavones in soybean cultivars subjected to flooding at various stages of development. The cultivars 'BRS267', 'BRS257' and 'BRS213' were subjected to 15 days of flooding, starting at the stages V6 and V8 and 11 days under stress starting at the stage R4. The proteins in the grain were extracted and quantified and analyzed by SDS-PAGE electrophoresis. Isoflavones were extracted, separated and quantified on HPLC. The electrophoretic analysis of the three cultivars under study revealed the same pattern of banding relative to the total protein regardless of the treatment. However, it was noted that flooding led to an increase in the total contents of isoflavones in the BRS 267 plants flooded in stage R4, remaining constant in other cultivars.O estresse imposto às plantas pelo alagamento do solo constitui uma barreira para o crescimento e produtividade destas. A identificação de cultivares de soja que possam produzir maior teor de isoflavonas torna-se necessária à medida que a soja vem sendo utilizada na alimentação humana e para redução dos riscos de doenças crônicas. Dessa forma, o presente estudo foi realizado com o objetivo de quantificar isoflavonas e proteínas em cultivares de soja submetidas ao alagamento em diferentes estádios de desenvolvimento. As cultivares 'BRS 267', 'BRS 257' e 'BRS 213' foram submetidas a 15 dias de alagamento, nos estádios V6 e V8 e 11 dias sob estresse, no estádio R4. As proteínas no grão foram extraídas e quantificadas e analisadas as eletroforeses em SDS-PAGE. Os isoflavonóides foram extraídos, separados e quantificados por HPLC. A análise por eletroforese das três cultivares revelou o mesmo padrão de bandas relativas às proteínas totais, independente do tratamento utilizado. O alagamento promoveu aumento no teor total de isoflavonas nas plantas alagadas no estádio R4 da cultivar 'BRS 267', permanecendo constante nas demais cultivares.Universidade Federal de Santa Maria2020-01-15T11:45:57Z2020-01-15T11:45:57Z2011-12info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfFANTE, C. A. et al. Isoflavone and protein content in soybeans grains submitted to flooding at different stages of development. Ciência Rural, Santa Maria, v. 41, n. 12, p. 2224-2229, Dec. 2011.http://repositorio.ufla.br/jspui/handle/1/38511Ciência Ruralreponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAAttribution-NonCommercial 4.0 Internationalhttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccessFante, Camila ArgentaGoulart, Patrícia de Fátima PereiraAlves, José DonizetiHenrique, Paôla de CastroFries, Daniela Deitoseng2020-01-15T11:45:58Zoai:localhost:1/38511Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2020-01-15T11:45:58Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false |
dc.title.none.fl_str_mv |
Isoflavone and protein content in soybeans grains submitted to flooding at different stages of development Teor de isoflavonas e proteínas nos grãos de soja submetida ao alagamento em diferentes estádios de desenvolvimento |
title |
Isoflavone and protein content in soybeans grains submitted to flooding at different stages of development |
spellingShingle |
Isoflavone and protein content in soybeans grains submitted to flooding at different stages of development Fante, Camila Argenta Glycine max Hypoxia Functional food Hipoxia Alimento funcional |
title_short |
Isoflavone and protein content in soybeans grains submitted to flooding at different stages of development |
title_full |
Isoflavone and protein content in soybeans grains submitted to flooding at different stages of development |
title_fullStr |
Isoflavone and protein content in soybeans grains submitted to flooding at different stages of development |
title_full_unstemmed |
Isoflavone and protein content in soybeans grains submitted to flooding at different stages of development |
title_sort |
Isoflavone and protein content in soybeans grains submitted to flooding at different stages of development |
author |
Fante, Camila Argenta |
author_facet |
Fante, Camila Argenta Goulart, Patrícia de Fátima Pereira Alves, José Donizeti Henrique, Paôla de Castro Fries, Daniela Deitos |
author_role |
author |
author2 |
Goulart, Patrícia de Fátima Pereira Alves, José Donizeti Henrique, Paôla de Castro Fries, Daniela Deitos |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Fante, Camila Argenta Goulart, Patrícia de Fátima Pereira Alves, José Donizeti Henrique, Paôla de Castro Fries, Daniela Deitos |
dc.subject.por.fl_str_mv |
Glycine max Hypoxia Functional food Hipoxia Alimento funcional |
topic |
Glycine max Hypoxia Functional food Hipoxia Alimento funcional |
description |
The stress imposed on plants by soil flooding constitutes a major barrier to growth and productivity. The identification of soybean varieties that produce higher levels of isoflavones, is necessary as soybeans have been used as human food to reduce risks of chronic diseases. Thus, this study was conducted with the objective of quantifying proteins and isoflavones in soybean cultivars subjected to flooding at various stages of development. The cultivars 'BRS267', 'BRS257' and 'BRS213' were subjected to 15 days of flooding, starting at the stages V6 and V8 and 11 days under stress starting at the stage R4. The proteins in the grain were extracted and quantified and analyzed by SDS-PAGE electrophoresis. Isoflavones were extracted, separated and quantified on HPLC. The electrophoretic analysis of the three cultivars under study revealed the same pattern of banding relative to the total protein regardless of the treatment. However, it was noted that flooding led to an increase in the total contents of isoflavones in the BRS 267 plants flooded in stage R4, remaining constant in other cultivars. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-12 2020-01-15T11:45:57Z 2020-01-15T11:45:57Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
FANTE, C. A. et al. Isoflavone and protein content in soybeans grains submitted to flooding at different stages of development. Ciência Rural, Santa Maria, v. 41, n. 12, p. 2224-2229, Dec. 2011. http://repositorio.ufla.br/jspui/handle/1/38511 |
identifier_str_mv |
FANTE, C. A. et al. Isoflavone and protein content in soybeans grains submitted to flooding at different stages of development. Ciência Rural, Santa Maria, v. 41, n. 12, p. 2224-2229, Dec. 2011. |
url |
http://repositorio.ufla.br/jspui/handle/1/38511 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
Attribution-NonCommercial 4.0 International http://creativecommons.org/licenses/by-nc/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution-NonCommercial 4.0 International http://creativecommons.org/licenses/by-nc/4.0/ |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
dc.source.none.fl_str_mv |
Ciência Rural reponame:Repositório Institucional da UFLA instname:Universidade Federal de Lavras (UFLA) instacron:UFLA |
instname_str |
Universidade Federal de Lavras (UFLA) |
instacron_str |
UFLA |
institution |
UFLA |
reponame_str |
Repositório Institucional da UFLA |
collection |
Repositório Institucional da UFLA |
repository.name.fl_str_mv |
Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA) |
repository.mail.fl_str_mv |
nivaldo@ufla.br || repositorio.biblioteca@ufla.br |
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1784550170296320000 |