Karyotype analysis, DNA content and molecular screening in Lippia alba (Verbenaceae)

Detalhes bibliográficos
Autor(a) principal: Pierre, Patrícia M. O.
Data de Publicação: 2011
Outros Autores: Sousa, Saulo M., Davide, Lisete C., Machado, Marco A., Viccini, Lyderson F.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFLA
Texto Completo: http://repositorio.ufla.br/jspui/handle/1/39006
Resumo: Cytogenetic analyses, of pollen viability, nuclear DNA content and RAPD markers were employed to study three chemotypes of Lippia alba (Mill.) (Verbenaceae) in order to understand the genetic variation among them. Different ploidy levels and mixoploid individuals were observed. This work comprises the first report of different chromosome numbers (cytotypes) in L. alba. The chromosome numbers of La2-carvone and La3-linalool chemotypes suggested that they are polyploids. Flow cytometric analysis showed an increase of nuclear DNA content that was not directly proportional to ploidy level variation. A cluster analysis based on RAPD markers revealed that La3-linalool shares genetic markers with La1-citral and La2-carvone. The analysis showed that the majority of genetic variation of La3-linalool could be a consequence of ixoploidy. ur data indicates that sexual reproduction aong those three chemotypes is unlikely and suggests the beginning of reproductive isolation. The results demonstrated that chromosome analysis, nuclear DNA content estimation and RAPD markers constitute excellent tools for detecting genetic variation among L. alba chemotypes.
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spelling Karyotype analysis, DNA content and molecular screening in Lippia alba (Verbenaceae)Cytogenetic analysesPollen viabilityDNA contentLippia albaAnálises citogenéticasViabilidade do pólenConteúdo de DNACytogenetic analyses, of pollen viability, nuclear DNA content and RAPD markers were employed to study three chemotypes of Lippia alba (Mill.) (Verbenaceae) in order to understand the genetic variation among them. Different ploidy levels and mixoploid individuals were observed. This work comprises the first report of different chromosome numbers (cytotypes) in L. alba. The chromosome numbers of La2-carvone and La3-linalool chemotypes suggested that they are polyploids. Flow cytometric analysis showed an increase of nuclear DNA content that was not directly proportional to ploidy level variation. A cluster analysis based on RAPD markers revealed that La3-linalool shares genetic markers with La1-citral and La2-carvone. The analysis showed that the majority of genetic variation of La3-linalool could be a consequence of ixoploidy. ur data indicates that sexual reproduction aong those three chemotypes is unlikely and suggests the beginning of reproductive isolation. The results demonstrated that chromosome analysis, nuclear DNA content estimation and RAPD markers constitute excellent tools for detecting genetic variation among L. alba chemotypes.Análises citogenéticas, de viabilidade do pólen, do conteúdo de DNA nuclear e marcadores RAPD foram empregadas no estudo de três quimiotipos de Lippia alba (Mill.) (Verbenaceae) visando contribuir para o entendimento da variação genética entre os mesmos. Diferentes níveis de ploidia e indivíduos mixoploides foram observados. Este trabalho compreende o primeiro relato de diferentes números cromossômicos (citótipos) em L. alba. Os números cromossômicos dos quimiotipos La2-carvona e La3-linalol sugere que eles seja poliploides. A análise da citometria de fluxo mostrou um aumento do conteúdo de DNA nuclear que não foi diretamente proporcional à variação no nível de ploidia. A análise de agrupamento baseada nos marcadores RAPD demonstrou que La3-linalol compartilha marcadores genéticos com La1-citral e La2-carvona. A análise mostrou que a maior parte da variação genética de La3-linalol pode ser consequência da mixoploidia. Nossos dados indicam que a reprodução sexual entre os três quimiotipos parece improvável, sugerindo o início de isolamento reprodutivo. Os resultados demonstraram que a análise cromossômica, a quantificação do DNA nuclear estimado e os marcadores RAPD constituem excelentes ferramentas para detecção de variação genética entre quimiotipos de L. alba.Academia Brasileira de Ciências2020-02-12T14:41:41Z2020-02-12T14:41:41Z2011info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfPIERRE, P. M. O.; SOUSA, S. M.; DAVIDE, L. C.; MACHADO, M. A.; VICCINI, L. F. Karyotype analysis, DNA content and molecular screening in Lippia alba (Verbenaceae). Anais da Academia Brasileira de Ciências, Rio de Janeiro, v. 83, n. 3, p. 993-1005, 2011.http://repositorio.ufla.br/jspui/handle/1/39006Anais da Academia Brasileira de Ciênciasreponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAAttribution-NonCommercial 4.0 Internationalhttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccessPierre, Patrícia M. O.Sousa, Saulo M.Davide, Lisete C.Machado, Marco A.Viccini, Lyderson F.eng2020-02-12T14:41:42Zoai:localhost:1/39006Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2020-02-12T14:41:42Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false
dc.title.none.fl_str_mv Karyotype analysis, DNA content and molecular screening in Lippia alba (Verbenaceae)
title Karyotype analysis, DNA content and molecular screening in Lippia alba (Verbenaceae)
spellingShingle Karyotype analysis, DNA content and molecular screening in Lippia alba (Verbenaceae)
Pierre, Patrícia M. O.
Cytogenetic analyses
Pollen viability
DNA content
Lippia alba
Análises citogenéticas
Viabilidade do pólen
Conteúdo de DNA
title_short Karyotype analysis, DNA content and molecular screening in Lippia alba (Verbenaceae)
title_full Karyotype analysis, DNA content and molecular screening in Lippia alba (Verbenaceae)
title_fullStr Karyotype analysis, DNA content and molecular screening in Lippia alba (Verbenaceae)
title_full_unstemmed Karyotype analysis, DNA content and molecular screening in Lippia alba (Verbenaceae)
title_sort Karyotype analysis, DNA content and molecular screening in Lippia alba (Verbenaceae)
author Pierre, Patrícia M. O.
author_facet Pierre, Patrícia M. O.
Sousa, Saulo M.
Davide, Lisete C.
Machado, Marco A.
Viccini, Lyderson F.
author_role author
author2 Sousa, Saulo M.
Davide, Lisete C.
Machado, Marco A.
Viccini, Lyderson F.
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Pierre, Patrícia M. O.
Sousa, Saulo M.
Davide, Lisete C.
Machado, Marco A.
Viccini, Lyderson F.
dc.subject.por.fl_str_mv Cytogenetic analyses
Pollen viability
DNA content
Lippia alba
Análises citogenéticas
Viabilidade do pólen
Conteúdo de DNA
topic Cytogenetic analyses
Pollen viability
DNA content
Lippia alba
Análises citogenéticas
Viabilidade do pólen
Conteúdo de DNA
description Cytogenetic analyses, of pollen viability, nuclear DNA content and RAPD markers were employed to study three chemotypes of Lippia alba (Mill.) (Verbenaceae) in order to understand the genetic variation among them. Different ploidy levels and mixoploid individuals were observed. This work comprises the first report of different chromosome numbers (cytotypes) in L. alba. The chromosome numbers of La2-carvone and La3-linalool chemotypes suggested that they are polyploids. Flow cytometric analysis showed an increase of nuclear DNA content that was not directly proportional to ploidy level variation. A cluster analysis based on RAPD markers revealed that La3-linalool shares genetic markers with La1-citral and La2-carvone. The analysis showed that the majority of genetic variation of La3-linalool could be a consequence of ixoploidy. ur data indicates that sexual reproduction aong those three chemotypes is unlikely and suggests the beginning of reproductive isolation. The results demonstrated that chromosome analysis, nuclear DNA content estimation and RAPD markers constitute excellent tools for detecting genetic variation among L. alba chemotypes.
publishDate 2011
dc.date.none.fl_str_mv 2011
2020-02-12T14:41:41Z
2020-02-12T14:41:41Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv PIERRE, P. M. O.; SOUSA, S. M.; DAVIDE, L. C.; MACHADO, M. A.; VICCINI, L. F. Karyotype analysis, DNA content and molecular screening in Lippia alba (Verbenaceae). Anais da Academia Brasileira de Ciências, Rio de Janeiro, v. 83, n. 3, p. 993-1005, 2011.
http://repositorio.ufla.br/jspui/handle/1/39006
identifier_str_mv PIERRE, P. M. O.; SOUSA, S. M.; DAVIDE, L. C.; MACHADO, M. A.; VICCINI, L. F. Karyotype analysis, DNA content and molecular screening in Lippia alba (Verbenaceae). Anais da Academia Brasileira de Ciências, Rio de Janeiro, v. 83, n. 3, p. 993-1005, 2011.
url http://repositorio.ufla.br/jspui/handle/1/39006
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv Attribution-NonCommercial 4.0 International
http://creativecommons.org/licenses/by-nc/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NonCommercial 4.0 International
http://creativecommons.org/licenses/by-nc/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Academia Brasileira de Ciências
publisher.none.fl_str_mv Academia Brasileira de Ciências
dc.source.none.fl_str_mv Anais da Academia Brasileira de Ciências
reponame:Repositório Institucional da UFLA
instname:Universidade Federal de Lavras (UFLA)
instacron:UFLA
instname_str Universidade Federal de Lavras (UFLA)
instacron_str UFLA
institution UFLA
reponame_str Repositório Institucional da UFLA
collection Repositório Institucional da UFLA
repository.name.fl_str_mv Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)
repository.mail.fl_str_mv nivaldo@ufla.br || repositorio.biblioteca@ufla.br
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