Efeito do inibidor de protease Kunitz sobre níveis de lipoxigenases em sementes de soja
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFLA |
Texto Completo: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542008000400014 http://repositorio.ufla.br/jspui/handle/1/6739 |
Resumo: | Lipoxygenases (LOX) are responsible for the beany flavor, which is the most important obstacle for the consumption of soybean [Glycine max. (L.) Merrill] products in Western countries. The Kunitz trypsin inhibitor (KTI) is responsible for 80% of the inhibition of tryptic activity in soybean seeds, and besides it can cause pancreatic hyperplasia in monogastric animals. These two traits are very important in soybean breeding programs that aim the improvement of soy protein quality. The objective of this study was to verify the influence of KTI on LOX levels in soybean seeds by analyzing the following genotypes: KTI+/LOX+, KTI+/LOX-, KTI-/LOX+ and KTI-/LOX-. LOX 1 and LOX 3 were identified by colorimetric, activity and electrophoretic assays. Protein content was determined by the bicinchoninic method for determination of LOX specific activity. For genotype KTI+/LOX+, LOX 1 specific activity was high varying from 177.92 to 206.94 AU/mg of protein and LOX 3 activity varied from 17.90 to 19.85 AU/mg of protein. No LOX activity was detected in genotypes KTI+/LOX- and KTI-/LOX-. For genotype KTI-/LOX+, LOX 1 activity varied from 117.24 to 124.64 AU/mg of protein, and the activity of LOX 3 from 17.35 to 20.29 AU/mg of protein. There was a reduction of 40% on LOX 1 specific activity and 1% for LOX 3 in the KTI-/LOX+ genotype in comparison to genotype KTI+/LOX+. These results indicate an inverse relationship between the presence of the Kunitz trypsin inhibitor in soybean seeds and lipoxygenase activity. |
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Efeito do inibidor de protease Kunitz sobre níveis de lipoxigenases em sementes de sojaEffect of protease inhibitor on the levels of lipoxygenase in soybean seedsGlycine maxTripsinaProteínaAtividade enzimáticaKTITrypsinProteinEnzymatic activityLipoxygenases (LOX) are responsible for the beany flavor, which is the most important obstacle for the consumption of soybean [Glycine max. (L.) Merrill] products in Western countries. The Kunitz trypsin inhibitor (KTI) is responsible for 80% of the inhibition of tryptic activity in soybean seeds, and besides it can cause pancreatic hyperplasia in monogastric animals. These two traits are very important in soybean breeding programs that aim the improvement of soy protein quality. The objective of this study was to verify the influence of KTI on LOX levels in soybean seeds by analyzing the following genotypes: KTI+/LOX+, KTI+/LOX-, KTI-/LOX+ and KTI-/LOX-. LOX 1 and LOX 3 were identified by colorimetric, activity and electrophoretic assays. Protein content was determined by the bicinchoninic method for determination of LOX specific activity. For genotype KTI+/LOX+, LOX 1 specific activity was high varying from 177.92 to 206.94 AU/mg of protein and LOX 3 activity varied from 17.90 to 19.85 AU/mg of protein. No LOX activity was detected in genotypes KTI+/LOX- and KTI-/LOX-. For genotype KTI-/LOX+, LOX 1 activity varied from 117.24 to 124.64 AU/mg of protein, and the activity of LOX 3 from 17.35 to 20.29 AU/mg of protein. There was a reduction of 40% on LOX 1 specific activity and 1% for LOX 3 in the KTI-/LOX+ genotype in comparison to genotype KTI+/LOX+. These results indicate an inverse relationship between the presence of the Kunitz trypsin inhibitor in soybean seeds and lipoxygenase activity.As lipoxigenases (LOX) são responsáveis pelo "beany flavor", sabor característico da soja [Glycine max. (L.) Merrill], que é o maior obstáculo ao consumo da soja nos países ocidentais. O inibidor de tripsina Kunitz (KTI) é responsável por 80% da inibição da atividade tríptica, além de provocar hiperplasia pancreática em animais monogástricos. Essas duas características são muito importantes em programas de melhoramento que visam à melhoria da qualidade da soja. Objetivou-se, neste trabalho verificar o efeito do inibidor de protease (KTI), sobre níveis de LOX em sementes de soja, nos seguintes genótipos: KTI+/LOX+, KTI+/LOX-, KTI-/LOX+ e KTI-/LOX-. As LOXs foram identificadas por teste colorimétrico, atividade enzimática e eletroforese em gel de poliacrilamida. O teor de proteína foi quantificado pelo método do ácido bicinconínico para determinação da atividade específica de LOX. Observou-se que, para o genótipo KTI+/LOX+, a atividade específica de LOX 1 foi alta, variando de 177,92 a 206,94 UA/mg de proteína e para LOX 3 variou de 17,90 a 19,85 UA/mg de proteína; para KTI+/LOX- e KTI-/LOX- não foi detectada atividade de LOX e que para o genótipo KTI-/LOX+ a atividade específica para LOX 1 variou de 117,24 a 124,64 UA/mg de proteína, e de 17,35 a 20,29 UA/mg de proteína para LOX 3. Houve uma redução de 40% na atividade específica de LOX1 e de 1% para LOX 3, no genótipo KTI-/LOX+, quando comparados ao KTI+/LOX+. Esses resultados comprovam uma associação negativa entre o inibidor de tripsina Kunitz e a atividade de lipoxigenases.Editora da Universidade Federal de Lavras2008-08-012015-04-30T13:34:47Z2015-04-30T13:34:47Z2015-04-30info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articletext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542008000400014BARROS, J. G. de A. et al. Efeito do inibidor de protease Kunitz sobre níveis de lipoxigenases em sementes de soja. Ciência e Agrotecnologia, Lavras, v. 32, n. 4, p. 1126-1132, jul./ago. 2008.http://repositorio.ufla.br/jspui/handle/1/6739Ciência e Agrotecnologia v.32 n.4 2008reponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLABarros, Josie Gomes de AlmeidaMoraes, Rita Maria Alves dePiovesan, Newton DenizBarros, Everaldo Gonçalves deMoreira, Maurilio Alvesporinfo:eu-repo/semantics/openAccess2016-06-02T14:32:40Zoai:localhost:1/6739Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2016-06-02T14:32:40Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false |
dc.title.none.fl_str_mv |
Efeito do inibidor de protease Kunitz sobre níveis de lipoxigenases em sementes de soja Effect of protease inhibitor on the levels of lipoxygenase in soybean seeds |
title |
Efeito do inibidor de protease Kunitz sobre níveis de lipoxigenases em sementes de soja |
spellingShingle |
Efeito do inibidor de protease Kunitz sobre níveis de lipoxigenases em sementes de soja Barros, Josie Gomes de Almeida Glycine max Tripsina Proteína Atividade enzimática KTI Trypsin Protein Enzymatic activity |
title_short |
Efeito do inibidor de protease Kunitz sobre níveis de lipoxigenases em sementes de soja |
title_full |
Efeito do inibidor de protease Kunitz sobre níveis de lipoxigenases em sementes de soja |
title_fullStr |
Efeito do inibidor de protease Kunitz sobre níveis de lipoxigenases em sementes de soja |
title_full_unstemmed |
Efeito do inibidor de protease Kunitz sobre níveis de lipoxigenases em sementes de soja |
title_sort |
Efeito do inibidor de protease Kunitz sobre níveis de lipoxigenases em sementes de soja |
author |
Barros, Josie Gomes de Almeida |
author_facet |
Barros, Josie Gomes de Almeida Moraes, Rita Maria Alves de Piovesan, Newton Deniz Barros, Everaldo Gonçalves de Moreira, Maurilio Alves |
author_role |
author |
author2 |
Moraes, Rita Maria Alves de Piovesan, Newton Deniz Barros, Everaldo Gonçalves de Moreira, Maurilio Alves |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Barros, Josie Gomes de Almeida Moraes, Rita Maria Alves de Piovesan, Newton Deniz Barros, Everaldo Gonçalves de Moreira, Maurilio Alves |
dc.subject.por.fl_str_mv |
Glycine max Tripsina Proteína Atividade enzimática KTI Trypsin Protein Enzymatic activity |
topic |
Glycine max Tripsina Proteína Atividade enzimática KTI Trypsin Protein Enzymatic activity |
description |
Lipoxygenases (LOX) are responsible for the beany flavor, which is the most important obstacle for the consumption of soybean [Glycine max. (L.) Merrill] products in Western countries. The Kunitz trypsin inhibitor (KTI) is responsible for 80% of the inhibition of tryptic activity in soybean seeds, and besides it can cause pancreatic hyperplasia in monogastric animals. These two traits are very important in soybean breeding programs that aim the improvement of soy protein quality. The objective of this study was to verify the influence of KTI on LOX levels in soybean seeds by analyzing the following genotypes: KTI+/LOX+, KTI+/LOX-, KTI-/LOX+ and KTI-/LOX-. LOX 1 and LOX 3 were identified by colorimetric, activity and electrophoretic assays. Protein content was determined by the bicinchoninic method for determination of LOX specific activity. For genotype KTI+/LOX+, LOX 1 specific activity was high varying from 177.92 to 206.94 AU/mg of protein and LOX 3 activity varied from 17.90 to 19.85 AU/mg of protein. No LOX activity was detected in genotypes KTI+/LOX- and KTI-/LOX-. For genotype KTI-/LOX+, LOX 1 activity varied from 117.24 to 124.64 AU/mg of protein, and the activity of LOX 3 from 17.35 to 20.29 AU/mg of protein. There was a reduction of 40% on LOX 1 specific activity and 1% for LOX 3 in the KTI-/LOX+ genotype in comparison to genotype KTI+/LOX+. These results indicate an inverse relationship between the presence of the Kunitz trypsin inhibitor in soybean seeds and lipoxygenase activity. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-08-01 2015-04-30T13:34:47Z 2015-04-30T13:34:47Z 2015-04-30 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542008000400014 BARROS, J. G. de A. et al. Efeito do inibidor de protease Kunitz sobre níveis de lipoxigenases em sementes de soja. Ciência e Agrotecnologia, Lavras, v. 32, n. 4, p. 1126-1132, jul./ago. 2008. http://repositorio.ufla.br/jspui/handle/1/6739 |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542008000400014 http://repositorio.ufla.br/jspui/handle/1/6739 |
identifier_str_mv |
BARROS, J. G. de A. et al. Efeito do inibidor de protease Kunitz sobre níveis de lipoxigenases em sementes de soja. Ciência e Agrotecnologia, Lavras, v. 32, n. 4, p. 1126-1132, jul./ago. 2008. |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
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openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Editora da Universidade Federal de Lavras |
publisher.none.fl_str_mv |
Editora da Universidade Federal de Lavras |
dc.source.none.fl_str_mv |
Ciência e Agrotecnologia v.32 n.4 2008 reponame:Repositório Institucional da UFLA instname:Universidade Federal de Lavras (UFLA) instacron:UFLA |
instname_str |
Universidade Federal de Lavras (UFLA) |
instacron_str |
UFLA |
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UFLA |
reponame_str |
Repositório Institucional da UFLA |
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Repositório Institucional da UFLA |
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Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA) |
repository.mail.fl_str_mv |
nivaldo@ufla.br || repositorio.biblioteca@ufla.br |
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1784550045337518080 |