Euphorbia trigona latex nematicidal activity on the root-knot nematode Meloidogyne incognita

Detalhes bibliográficos
Autor(a) principal: Sufiate, Bruna Leite
Data de Publicação: 2021
Outros Autores: Soares, Filippe Elias de Freitas, Ferreira, Thiago de Freitas, Queiroz, José Humberto de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFLA
Texto Completo: http://repositorio.ufla.br/jspui/handle/1/48919
Resumo: The aim of this study was to test nematicidal activity of the Euphorbiatrigona latex on Meloidogyne incognita juveniles and to partially purify and characterize three proteases present in thislatex. Three distinct proteaseswere partially purificated from E. trigonalatex. They were named here trigonin 1, 2 and 3. Their molecular weights were estimated at: 36, 31 and 29 kDa, for trigonin 1, 2 and 3, respectively. The pH and temperature that provided highest protease activity were pH values of 4.0, 6.0 and 9.0, and 70 °C. The crude extract containing the three proteases present in E. trigona latex reduced significantly (p < 0.01) the number of live M. incognita juvenilesin 96% after 24 hours treatment. The present study is the first report of E. trigona latex with nematicidal activity, therefore more studies regarding latex proteases action on nematodes are needed.
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spelling Euphorbia trigona latex nematicidal activity on the root-knot nematode Meloidogyne incognitaAtividade nematicida do látex de Euphorbia trigona sobre o nematoide das galhas Meloidogyne incognitaActividad nematicida del látex de Euphorbia trigonaen el nematodo agallador Meloidogyne incognitaNematicidalProteaseMeloidogyne incognitaTrigoninNematicidalProteasaTrigoninaThe aim of this study was to test nematicidal activity of the Euphorbiatrigona latex on Meloidogyne incognita juveniles and to partially purify and characterize three proteases present in thislatex. Three distinct proteaseswere partially purificated from E. trigonalatex. They were named here trigonin 1, 2 and 3. Their molecular weights were estimated at: 36, 31 and 29 kDa, for trigonin 1, 2 and 3, respectively. The pH and temperature that provided highest protease activity were pH values of 4.0, 6.0 and 9.0, and 70 °C. The crude extract containing the three proteases present in E. trigona latex reduced significantly (p < 0.01) the number of live M. incognita juvenilesin 96% after 24 hours treatment. The present study is the first report of E. trigona latex with nematicidal activity, therefore more studies regarding latex proteases action on nematodes are needed.O objetivo deste estudo foi testar a atividade nematicida do látex de Euphorbia trigona sobre juvenis de Meloidogyne incognita e purificar parcialmente e caracterizar três proteases presentes neste látex. Três proteases distintas foram parcialmente purificadas do látex de E. trigona. Eles foram nomeadas como trigonina 1, 2 e 3. Seus pesos moleculares foram estimados em: 36, 31 e 29 kDa, para trigonina 1, 2 e 3, respectivamente. O pH e a temperatura que proporcionaram maior atividade de protease foram os valores de pH 4,0, 6,0 e 9,0 e 70 °C. O extrato bruto contendo as três proteases presentes no látex de E. trigona reduziu significativamente (p <0,01) o número de juvenis vivos de M. incognita em 96% após 24 horas de tratamento. O presente estudo é o primeiro relato do látex de E. trigona com atividade nematicida, portanto, mais estudos sobre a ação das proteases do látex sobre os nematoides são necessários.Universidade Federal de Itajubá (UNIFEI)2022-01-20T01:40:06Z2022-01-20T01:40:06Z2021-07-24info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfSUFIATE, B. L. et al. Euphorbia trigona latex nematicidal activity on the root-knot nematode Meloidogyne incognita. Research, Society and Development, [S.l.], v. 10, n. 9, 2021. DOI: 10.33448/rsd-v10i9.18037.http://repositorio.ufla.br/jspui/handle/1/48919Research, Society and Developmentreponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessSufiate, Bruna LeiteSoares, Filippe Elias de FreitasFerreira, Thiago de FreitasQueiroz, José Humberto deeng2022-01-20T01:40:07Zoai:localhost:1/48919Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2022-01-20T01:40:07Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false
dc.title.none.fl_str_mv Euphorbia trigona latex nematicidal activity on the root-knot nematode Meloidogyne incognita
Atividade nematicida do látex de Euphorbia trigona sobre o nematoide das galhas Meloidogyne incognita
Actividad nematicida del látex de Euphorbia trigonaen el nematodo agallador Meloidogyne incognita
title Euphorbia trigona latex nematicidal activity on the root-knot nematode Meloidogyne incognita
spellingShingle Euphorbia trigona latex nematicidal activity on the root-knot nematode Meloidogyne incognita
Sufiate, Bruna Leite
Nematicidal
Protease
Meloidogyne incognita
Trigonin
Nematicidal
Proteasa
Trigonina
title_short Euphorbia trigona latex nematicidal activity on the root-knot nematode Meloidogyne incognita
title_full Euphorbia trigona latex nematicidal activity on the root-knot nematode Meloidogyne incognita
title_fullStr Euphorbia trigona latex nematicidal activity on the root-knot nematode Meloidogyne incognita
title_full_unstemmed Euphorbia trigona latex nematicidal activity on the root-knot nematode Meloidogyne incognita
title_sort Euphorbia trigona latex nematicidal activity on the root-knot nematode Meloidogyne incognita
author Sufiate, Bruna Leite
author_facet Sufiate, Bruna Leite
Soares, Filippe Elias de Freitas
Ferreira, Thiago de Freitas
Queiroz, José Humberto de
author_role author
author2 Soares, Filippe Elias de Freitas
Ferreira, Thiago de Freitas
Queiroz, José Humberto de
author2_role author
author
author
dc.contributor.author.fl_str_mv Sufiate, Bruna Leite
Soares, Filippe Elias de Freitas
Ferreira, Thiago de Freitas
Queiroz, José Humberto de
dc.subject.por.fl_str_mv Nematicidal
Protease
Meloidogyne incognita
Trigonin
Nematicidal
Proteasa
Trigonina
topic Nematicidal
Protease
Meloidogyne incognita
Trigonin
Nematicidal
Proteasa
Trigonina
description The aim of this study was to test nematicidal activity of the Euphorbiatrigona latex on Meloidogyne incognita juveniles and to partially purify and characterize three proteases present in thislatex. Three distinct proteaseswere partially purificated from E. trigonalatex. They were named here trigonin 1, 2 and 3. Their molecular weights were estimated at: 36, 31 and 29 kDa, for trigonin 1, 2 and 3, respectively. The pH and temperature that provided highest protease activity were pH values of 4.0, 6.0 and 9.0, and 70 °C. The crude extract containing the three proteases present in E. trigona latex reduced significantly (p < 0.01) the number of live M. incognita juvenilesin 96% after 24 hours treatment. The present study is the first report of E. trigona latex with nematicidal activity, therefore more studies regarding latex proteases action on nematodes are needed.
publishDate 2021
dc.date.none.fl_str_mv 2021-07-24
2022-01-20T01:40:06Z
2022-01-20T01:40:06Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv SUFIATE, B. L. et al. Euphorbia trigona latex nematicidal activity on the root-knot nematode Meloidogyne incognita. Research, Society and Development, [S.l.], v. 10, n. 9, 2021. DOI: 10.33448/rsd-v10i9.18037.
http://repositorio.ufla.br/jspui/handle/1/48919
identifier_str_mv SUFIATE, B. L. et al. Euphorbia trigona latex nematicidal activity on the root-knot nematode Meloidogyne incognita. Research, Society and Development, [S.l.], v. 10, n. 9, 2021. DOI: 10.33448/rsd-v10i9.18037.
url http://repositorio.ufla.br/jspui/handle/1/48919
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Itajubá (UNIFEI)
publisher.none.fl_str_mv Universidade Federal de Itajubá (UNIFEI)
dc.source.none.fl_str_mv Research, Society and Development
reponame:Repositório Institucional da UFLA
instname:Universidade Federal de Lavras (UFLA)
instacron:UFLA
instname_str Universidade Federal de Lavras (UFLA)
instacron_str UFLA
institution UFLA
reponame_str Repositório Institucional da UFLA
collection Repositório Institucional da UFLA
repository.name.fl_str_mv Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)
repository.mail.fl_str_mv nivaldo@ufla.br || repositorio.biblioteca@ufla.br
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