Euphorbia trigona latex nematicidal activity on the root-knot nematode Meloidogyne incognita
Autor(a) principal: | |
---|---|
Data de Publicação: | 2021 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFLA |
Texto Completo: | http://repositorio.ufla.br/jspui/handle/1/48919 |
Resumo: | The aim of this study was to test nematicidal activity of the Euphorbiatrigona latex on Meloidogyne incognita juveniles and to partially purify and characterize three proteases present in thislatex. Three distinct proteaseswere partially purificated from E. trigonalatex. They were named here trigonin 1, 2 and 3. Their molecular weights were estimated at: 36, 31 and 29 kDa, for trigonin 1, 2 and 3, respectively. The pH and temperature that provided highest protease activity were pH values of 4.0, 6.0 and 9.0, and 70 °C. The crude extract containing the three proteases present in E. trigona latex reduced significantly (p < 0.01) the number of live M. incognita juvenilesin 96% after 24 hours treatment. The present study is the first report of E. trigona latex with nematicidal activity, therefore more studies regarding latex proteases action on nematodes are needed. |
id |
UFLA_d31123551558fd600073b9e876a21b40 |
---|---|
oai_identifier_str |
oai:localhost:1/48919 |
network_acronym_str |
UFLA |
network_name_str |
Repositório Institucional da UFLA |
repository_id_str |
|
spelling |
Euphorbia trigona latex nematicidal activity on the root-knot nematode Meloidogyne incognitaAtividade nematicida do látex de Euphorbia trigona sobre o nematoide das galhas Meloidogyne incognitaActividad nematicida del látex de Euphorbia trigonaen el nematodo agallador Meloidogyne incognitaNematicidalProteaseMeloidogyne incognitaTrigoninNematicidalProteasaTrigoninaThe aim of this study was to test nematicidal activity of the Euphorbiatrigona latex on Meloidogyne incognita juveniles and to partially purify and characterize three proteases present in thislatex. Three distinct proteaseswere partially purificated from E. trigonalatex. They were named here trigonin 1, 2 and 3. Their molecular weights were estimated at: 36, 31 and 29 kDa, for trigonin 1, 2 and 3, respectively. The pH and temperature that provided highest protease activity were pH values of 4.0, 6.0 and 9.0, and 70 °C. The crude extract containing the three proteases present in E. trigona latex reduced significantly (p < 0.01) the number of live M. incognita juvenilesin 96% after 24 hours treatment. The present study is the first report of E. trigona latex with nematicidal activity, therefore more studies regarding latex proteases action on nematodes are needed.O objetivo deste estudo foi testar a atividade nematicida do látex de Euphorbia trigona sobre juvenis de Meloidogyne incognita e purificar parcialmente e caracterizar três proteases presentes neste látex. Três proteases distintas foram parcialmente purificadas do látex de E. trigona. Eles foram nomeadas como trigonina 1, 2 e 3. Seus pesos moleculares foram estimados em: 36, 31 e 29 kDa, para trigonina 1, 2 e 3, respectivamente. O pH e a temperatura que proporcionaram maior atividade de protease foram os valores de pH 4,0, 6,0 e 9,0 e 70 °C. O extrato bruto contendo as três proteases presentes no látex de E. trigona reduziu significativamente (p <0,01) o número de juvenis vivos de M. incognita em 96% após 24 horas de tratamento. O presente estudo é o primeiro relato do látex de E. trigona com atividade nematicida, portanto, mais estudos sobre a ação das proteases do látex sobre os nematoides são necessários.Universidade Federal de Itajubá (UNIFEI)2022-01-20T01:40:06Z2022-01-20T01:40:06Z2021-07-24info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfSUFIATE, B. L. et al. Euphorbia trigona latex nematicidal activity on the root-knot nematode Meloidogyne incognita. Research, Society and Development, [S.l.], v. 10, n. 9, 2021. DOI: 10.33448/rsd-v10i9.18037.http://repositorio.ufla.br/jspui/handle/1/48919Research, Society and Developmentreponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessSufiate, Bruna LeiteSoares, Filippe Elias de FreitasFerreira, Thiago de FreitasQueiroz, José Humberto deeng2022-01-20T01:40:07Zoai:localhost:1/48919Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2022-01-20T01:40:07Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false |
dc.title.none.fl_str_mv |
Euphorbia trigona latex nematicidal activity on the root-knot nematode Meloidogyne incognita Atividade nematicida do látex de Euphorbia trigona sobre o nematoide das galhas Meloidogyne incognita Actividad nematicida del látex de Euphorbia trigonaen el nematodo agallador Meloidogyne incognita |
title |
Euphorbia trigona latex nematicidal activity on the root-knot nematode Meloidogyne incognita |
spellingShingle |
Euphorbia trigona latex nematicidal activity on the root-knot nematode Meloidogyne incognita Sufiate, Bruna Leite Nematicidal Protease Meloidogyne incognita Trigonin Nematicidal Proteasa Trigonina |
title_short |
Euphorbia trigona latex nematicidal activity on the root-knot nematode Meloidogyne incognita |
title_full |
Euphorbia trigona latex nematicidal activity on the root-knot nematode Meloidogyne incognita |
title_fullStr |
Euphorbia trigona latex nematicidal activity on the root-knot nematode Meloidogyne incognita |
title_full_unstemmed |
Euphorbia trigona latex nematicidal activity on the root-knot nematode Meloidogyne incognita |
title_sort |
Euphorbia trigona latex nematicidal activity on the root-knot nematode Meloidogyne incognita |
author |
Sufiate, Bruna Leite |
author_facet |
Sufiate, Bruna Leite Soares, Filippe Elias de Freitas Ferreira, Thiago de Freitas Queiroz, José Humberto de |
author_role |
author |
author2 |
Soares, Filippe Elias de Freitas Ferreira, Thiago de Freitas Queiroz, José Humberto de |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Sufiate, Bruna Leite Soares, Filippe Elias de Freitas Ferreira, Thiago de Freitas Queiroz, José Humberto de |
dc.subject.por.fl_str_mv |
Nematicidal Protease Meloidogyne incognita Trigonin Nematicidal Proteasa Trigonina |
topic |
Nematicidal Protease Meloidogyne incognita Trigonin Nematicidal Proteasa Trigonina |
description |
The aim of this study was to test nematicidal activity of the Euphorbiatrigona latex on Meloidogyne incognita juveniles and to partially purify and characterize three proteases present in thislatex. Three distinct proteaseswere partially purificated from E. trigonalatex. They were named here trigonin 1, 2 and 3. Their molecular weights were estimated at: 36, 31 and 29 kDa, for trigonin 1, 2 and 3, respectively. The pH and temperature that provided highest protease activity were pH values of 4.0, 6.0 and 9.0, and 70 °C. The crude extract containing the three proteases present in E. trigona latex reduced significantly (p < 0.01) the number of live M. incognita juvenilesin 96% after 24 hours treatment. The present study is the first report of E. trigona latex with nematicidal activity, therefore more studies regarding latex proteases action on nematodes are needed. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-07-24 2022-01-20T01:40:06Z 2022-01-20T01:40:06Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
SUFIATE, B. L. et al. Euphorbia trigona latex nematicidal activity on the root-knot nematode Meloidogyne incognita. Research, Society and Development, [S.l.], v. 10, n. 9, 2021. DOI: 10.33448/rsd-v10i9.18037. http://repositorio.ufla.br/jspui/handle/1/48919 |
identifier_str_mv |
SUFIATE, B. L. et al. Euphorbia trigona latex nematicidal activity on the root-knot nematode Meloidogyne incognita. Research, Society and Development, [S.l.], v. 10, n. 9, 2021. DOI: 10.33448/rsd-v10i9.18037. |
url |
http://repositorio.ufla.br/jspui/handle/1/48919 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/ |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Itajubá (UNIFEI) |
publisher.none.fl_str_mv |
Universidade Federal de Itajubá (UNIFEI) |
dc.source.none.fl_str_mv |
Research, Society and Development reponame:Repositório Institucional da UFLA instname:Universidade Federal de Lavras (UFLA) instacron:UFLA |
instname_str |
Universidade Federal de Lavras (UFLA) |
instacron_str |
UFLA |
institution |
UFLA |
reponame_str |
Repositório Institucional da UFLA |
collection |
Repositório Institucional da UFLA |
repository.name.fl_str_mv |
Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA) |
repository.mail.fl_str_mv |
nivaldo@ufla.br || repositorio.biblioteca@ufla.br |
_version_ |
1807835078514966528 |