Compostos orgânicos voláteis emitidos por Fusarium oxysporum apresentam atividade nematicida
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFLA |
Texto Completo: | http://repositorio.ufla.br/jspui/handle/1/11852 |
Resumo: | A series of assays was carried out aiming to understand the nematicidal activity of the volatile organic compounds (VOCs) emitted by Fusarium oxysporum isolated 21 (Fo-21) on plant-parasitic nematodes. Through analysis by gas chromatography coupled to mass spectrometry (GC-MS) 50 compounds were detected in the Fo-21 emission. The main classes of compounds emitted by Fo-21 were sesquiterpenes, esters and alcohols. Water exposed to VOCs caused 100% immobility in second-stage juveniles (J 2) of Meloidogyne incognita. In addition, inoculation of those J2 in tomato decreased infectivity up to 70% and reproduction up to 65%. Application of water exposed to VOCs in substrate infested with M. incognita J 2 resulted in 56% reduction in number of galls and 49% in number of eggs. The eight most abundant VOCs emitted by F.o–21 were tested against M. incognita and Heterodera schachtii. Compounds 2– methylbutyl acetate (1), 3-methylbutyl acetate (2), ethyl acetate (7) and, 2-methylpropyl acetate (8), caused in vitro mortality of 100%, 92.5%, 100% and 83.8%, respectively, in M. incognita and 90.0%, 88.8%, 96.3% and 97.5% mortality, respectively, on H. schachtii J 2, when tested at a concentration of 500 µg.ml -1 . The lethal concentration (LC50) for compounds 1, 2, 7 and 8, in M. incognita J 2 was of 236, 198, 213 and 218 µg.ml -1 , respectively. Under the same conditions, the commercial nematicide carbofuran (2,3-dihydro-2,2-dimethyl-1-benzofuran-7-yl N-methylcarbamate) showed a LC50 of 191 µg.ml -1 . Eggs exposed to compounds 1, 2, 7 and 8, for 72 h showed a reduction in hatching of 36.5%, 55.2%, 91.0% and 10.3%, respectively, and reduction in infectivity of 53.0%, 53.0%, 32.0 % and 39.0%, respectively. When compounds were applied in tomato seedlings infested by M. incognita, compound 1 reduced the number of galls per gram of root in 27.5% when compared to the negative control (without the application of nematicide). |
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Compostos orgânicos voláteis emitidos por Fusarium oxysporum apresentam atividade nematicidaVolatiles organic compounds emitted by Fusarium shows nematicidal activityNematóides de plantasFungos como agentes de controle da praga biológicaCompostos orgânicosPlant nematodesFungi as biological pest control agentsOrganic compoundsFusarium oxysporumMeloidogyne incognitaHeterodera schachtiiFitopatologiaA series of assays was carried out aiming to understand the nematicidal activity of the volatile organic compounds (VOCs) emitted by Fusarium oxysporum isolated 21 (Fo-21) on plant-parasitic nematodes. Through analysis by gas chromatography coupled to mass spectrometry (GC-MS) 50 compounds were detected in the Fo-21 emission. The main classes of compounds emitted by Fo-21 were sesquiterpenes, esters and alcohols. Water exposed to VOCs caused 100% immobility in second-stage juveniles (J 2) of Meloidogyne incognita. In addition, inoculation of those J2 in tomato decreased infectivity up to 70% and reproduction up to 65%. Application of water exposed to VOCs in substrate infested with M. incognita J 2 resulted in 56% reduction in number of galls and 49% in number of eggs. The eight most abundant VOCs emitted by F.o–21 were tested against M. incognita and Heterodera schachtii. Compounds 2– methylbutyl acetate (1), 3-methylbutyl acetate (2), ethyl acetate (7) and, 2-methylpropyl acetate (8), caused in vitro mortality of 100%, 92.5%, 100% and 83.8%, respectively, in M. incognita and 90.0%, 88.8%, 96.3% and 97.5% mortality, respectively, on H. schachtii J 2, when tested at a concentration of 500 µg.ml -1 . The lethal concentration (LC50) for compounds 1, 2, 7 and 8, in M. incognita J 2 was of 236, 198, 213 and 218 µg.ml -1 , respectively. Under the same conditions, the commercial nematicide carbofuran (2,3-dihydro-2,2-dimethyl-1-benzofuran-7-yl N-methylcarbamate) showed a LC50 of 191 µg.ml -1 . Eggs exposed to compounds 1, 2, 7 and 8, for 72 h showed a reduction in hatching of 36.5%, 55.2%, 91.0% and 10.3%, respectively, and reduction in infectivity of 53.0%, 53.0%, 32.0 % and 39.0%, respectively. When compounds were applied in tomato seedlings infested by M. incognita, compound 1 reduced the number of galls per gram of root in 27.5% when compared to the negative control (without the application of nematicide).Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Ensaios foram realizados para se entender a atuação dos compostos orgânicos voláteis (COVs) emitidos por Fusarium oxysporum isolado 21 (Fo-21) sobre os fitonematoides. Através da análise dos compostos por cromatografia gasosa acoplada espectrometria de massas (CG-MS), foram detectados 50 compostos nas emissões de Fo-21. As principais classes de compostos emitidos foram sesquiterpenos, ésteres e alcoóis. A água exposta aos COVs de Fo-21 causou 100% de imobilidade em juvenis de segundo estádio (J 2) de Meloidogyne incognita, enquanto a inoculação em tomateiro desses J 2 resultou em redução de até 70% da infectividade e de até 65% da reprodução. A aplicação da água exposta aos COVs em substrato infestado com J 2 de M. incognita resultou em 56% de redução no número de galhas e 49% no número de ovos. A atividade nematicida dos oito COVs emitidos em maior abundância por F.o-21 foi avaliada in vitro sobre M. incognita e Heterodera schachtii. Os compostos acetato de 2-metilbutila (1), acetato de 3-metilbutila (2), acetato de etila (7), acetato de 2 – metilpropila (8), na concentração de 500 µg.ml -1 , causaram mortalidades entre 100% e 83,8%, em J 2 de M. incognita e H. schachtii. A concentração letal (CL50) dos compostos para J 2 de M. incognita variou entre 236 e 198 µg.ml -1 , enquanto que nas mesmas condições o nematicida comercial carbofuran (2,3-dihydro-2,2-dimethyl-1-benzofuran-7-yl N-methylcarbamate) foi de 191 µg.ml -1 . Ovos expostos aos compostos 1, 2, 7 e 8, por 72 h, tiveram reduzidas a eclosão do J 2 em 36,5%, 55,2%, 91,0% e 10,3%, e a infectividade de 53%, 53%, 32% e 39%, respectivamente. Quando os compostos foram aplicados em mudas de tomateiro infestadas com M. incognita o composto 1 reduziu o número de galhas por grama de raiz em 27,5% quando comparado com o controle negativo sem aplicação de nematicida.Universidade Federal de LavrasPrograma de Pós-Graduação em Agronomia/FitopatologiaUFLAbrasilDepartamento de FitopatologiaCampos, Vicente PauloPfenning, Ludwig HeinrichPedroso, Marcio PozzobonFreire, Eduardo SouzaSalustiano, Maria EloisaTerra, Willian César2016-10-04T13:20:15Z2016-10-04T13:20:15Z2016-10-032016-09-12info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisapplication/pdfTERRA, W. C. Compostos orgânicos voláteis emitidos por Fusarium oxysporum apresentam atividade nematicida. 2016. 68 p. Tese (Doutorado em Agronomia/Fitopatologia)-Universidade Federal de Lavras, Lavras, 2016.http://repositorio.ufla.br/jspui/handle/1/11852porinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLA2016-10-04T13:20:15Zoai:localhost:1/11852Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2016-10-04T13:20:15Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false |
dc.title.none.fl_str_mv |
Compostos orgânicos voláteis emitidos por Fusarium oxysporum apresentam atividade nematicida Volatiles organic compounds emitted by Fusarium shows nematicidal activity |
title |
Compostos orgânicos voláteis emitidos por Fusarium oxysporum apresentam atividade nematicida |
spellingShingle |
Compostos orgânicos voláteis emitidos por Fusarium oxysporum apresentam atividade nematicida Terra, Willian César Nematóides de plantas Fungos como agentes de controle da praga biológica Compostos orgânicos Plant nematodes Fungi as biological pest control agents Organic compounds Fusarium oxysporum Meloidogyne incognita Heterodera schachtii Fitopatologia |
title_short |
Compostos orgânicos voláteis emitidos por Fusarium oxysporum apresentam atividade nematicida |
title_full |
Compostos orgânicos voláteis emitidos por Fusarium oxysporum apresentam atividade nematicida |
title_fullStr |
Compostos orgânicos voláteis emitidos por Fusarium oxysporum apresentam atividade nematicida |
title_full_unstemmed |
Compostos orgânicos voláteis emitidos por Fusarium oxysporum apresentam atividade nematicida |
title_sort |
Compostos orgânicos voláteis emitidos por Fusarium oxysporum apresentam atividade nematicida |
author |
Terra, Willian César |
author_facet |
Terra, Willian César |
author_role |
author |
dc.contributor.none.fl_str_mv |
Campos, Vicente Paulo Pfenning, Ludwig Heinrich Pedroso, Marcio Pozzobon Freire, Eduardo Souza Salustiano, Maria Eloisa |
dc.contributor.author.fl_str_mv |
Terra, Willian César |
dc.subject.por.fl_str_mv |
Nematóides de plantas Fungos como agentes de controle da praga biológica Compostos orgânicos Plant nematodes Fungi as biological pest control agents Organic compounds Fusarium oxysporum Meloidogyne incognita Heterodera schachtii Fitopatologia |
topic |
Nematóides de plantas Fungos como agentes de controle da praga biológica Compostos orgânicos Plant nematodes Fungi as biological pest control agents Organic compounds Fusarium oxysporum Meloidogyne incognita Heterodera schachtii Fitopatologia |
description |
A series of assays was carried out aiming to understand the nematicidal activity of the volatile organic compounds (VOCs) emitted by Fusarium oxysporum isolated 21 (Fo-21) on plant-parasitic nematodes. Through analysis by gas chromatography coupled to mass spectrometry (GC-MS) 50 compounds were detected in the Fo-21 emission. The main classes of compounds emitted by Fo-21 were sesquiterpenes, esters and alcohols. Water exposed to VOCs caused 100% immobility in second-stage juveniles (J 2) of Meloidogyne incognita. In addition, inoculation of those J2 in tomato decreased infectivity up to 70% and reproduction up to 65%. Application of water exposed to VOCs in substrate infested with M. incognita J 2 resulted in 56% reduction in number of galls and 49% in number of eggs. The eight most abundant VOCs emitted by F.o–21 were tested against M. incognita and Heterodera schachtii. Compounds 2– methylbutyl acetate (1), 3-methylbutyl acetate (2), ethyl acetate (7) and, 2-methylpropyl acetate (8), caused in vitro mortality of 100%, 92.5%, 100% and 83.8%, respectively, in M. incognita and 90.0%, 88.8%, 96.3% and 97.5% mortality, respectively, on H. schachtii J 2, when tested at a concentration of 500 µg.ml -1 . The lethal concentration (LC50) for compounds 1, 2, 7 and 8, in M. incognita J 2 was of 236, 198, 213 and 218 µg.ml -1 , respectively. Under the same conditions, the commercial nematicide carbofuran (2,3-dihydro-2,2-dimethyl-1-benzofuran-7-yl N-methylcarbamate) showed a LC50 of 191 µg.ml -1 . Eggs exposed to compounds 1, 2, 7 and 8, for 72 h showed a reduction in hatching of 36.5%, 55.2%, 91.0% and 10.3%, respectively, and reduction in infectivity of 53.0%, 53.0%, 32.0 % and 39.0%, respectively. When compounds were applied in tomato seedlings infested by M. incognita, compound 1 reduced the number of galls per gram of root in 27.5% when compared to the negative control (without the application of nematicide). |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-10-04T13:20:15Z 2016-10-04T13:20:15Z 2016-10-03 2016-09-12 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
TERRA, W. C. Compostos orgânicos voláteis emitidos por Fusarium oxysporum apresentam atividade nematicida. 2016. 68 p. Tese (Doutorado em Agronomia/Fitopatologia)-Universidade Federal de Lavras, Lavras, 2016. http://repositorio.ufla.br/jspui/handle/1/11852 |
identifier_str_mv |
TERRA, W. C. Compostos orgânicos voláteis emitidos por Fusarium oxysporum apresentam atividade nematicida. 2016. 68 p. Tese (Doutorado em Agronomia/Fitopatologia)-Universidade Federal de Lavras, Lavras, 2016. |
url |
http://repositorio.ufla.br/jspui/handle/1/11852 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Lavras Programa de Pós-Graduação em Agronomia/Fitopatologia UFLA brasil Departamento de Fitopatologia |
publisher.none.fl_str_mv |
Universidade Federal de Lavras Programa de Pós-Graduação em Agronomia/Fitopatologia UFLA brasil Departamento de Fitopatologia |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFLA instname:Universidade Federal de Lavras (UFLA) instacron:UFLA |
instname_str |
Universidade Federal de Lavras (UFLA) |
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UFLA |
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UFLA |
reponame_str |
Repositório Institucional da UFLA |
collection |
Repositório Institucional da UFLA |
repository.name.fl_str_mv |
Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA) |
repository.mail.fl_str_mv |
nivaldo@ufla.br || repositorio.biblioteca@ufla.br |
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1784549972884062208 |