Efeito do BAP no cultivo in vitro de Ocimum selloi Benth
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFLA |
Texto Completo: | http://repositorio.ufla.br/jspui/handle/1/38317 |
Resumo: | The present study was undertaken to develop the proliferation of sprouts in apical and nodal segments of Ocimum selloi with different BAP levels. Young plants aged 60 days were used as donors of nodal and apical segments. The segments were inoculated in MS medium at half the concentration of salts supplemented with 1.5% of sucrose and different BAP levels. The experiment was in 3 x 4 factorial arrangement, 3 positions of O. selloi segments (apical segment; first and second nodal segment) and 3 BAP levels (0 - control; 2; 4 and 6 mg L-1). After 30 days, the number, the length, and the fresh and dry biomass of sprouts and roots were evaluated. The first and the second segments showed better results in inducing O. selloi sprouts, 7 and 8 sprouts/explant, at the different BAP levels, but there was not root formation in the presence of the cytokinin. Under the tested conditions, use of the first and the second nodal segments is recommended in addition to supplementing the culture medium with BAP for in vitro proliferation of O. selloi sprouts. |
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Efeito do BAP no cultivo in vitro de Ocimum selloi BenthEffect of BAP on in vitro culture of Ocimum selloi BenthPlantas medicinaisBenzilaminopurinaAtroveran - MicropropagationMedicinal plantsBenzylaminopurineAtroveran - MicropropagaçãoThe present study was undertaken to develop the proliferation of sprouts in apical and nodal segments of Ocimum selloi with different BAP levels. Young plants aged 60 days were used as donors of nodal and apical segments. The segments were inoculated in MS medium at half the concentration of salts supplemented with 1.5% of sucrose and different BAP levels. The experiment was in 3 x 4 factorial arrangement, 3 positions of O. selloi segments (apical segment; first and second nodal segment) and 3 BAP levels (0 - control; 2; 4 and 6 mg L-1). After 30 days, the number, the length, and the fresh and dry biomass of sprouts and roots were evaluated. The first and the second segments showed better results in inducing O. selloi sprouts, 7 and 8 sprouts/explant, at the different BAP levels, but there was not root formation in the presence of the cytokinin. Under the tested conditions, use of the first and the second nodal segments is recommended in addition to supplementing the culture medium with BAP for in vitro proliferation of O. selloi sprouts.Visando promover a proliferação de brotações em segmentos apicais e nodais de Ocimum selloi em diferentes concentrações de BAP, plantas jovens de 60 dias serviram de doadoras de segmentos apicais e nodais. Os segmentos foram inoculados em meio MS preparado com a metade da concentração dos sais, e acrescido de 1,5% de sacarose e diferentes concentrações de BAP. O experimento foi conduzido no delineamento fatorial, 3 x 4, sendo 3 as posições dos segmentos de O. selloi (segmento apical, primeiro e segundo segmentos nodais) e 4 concentrações de BAP (0 - controle; 2; 4 e 6 mg L-1). Aos 30 dias, foram avaliados o número, comprimento e biomassa fresca e seca de brotos e raízes. Os primeiros e segundos segmentos apresentaram melhores resultados na indução de brotos de O. selloi, 7 e 8 brotos/explante, nas diferentes concentrações de BAP; porém, não houve formação de raízes na presença da citocinina. Nas condições testadas, recomenda-se o uso do primeiro e segundo segmento nodal suplementando o meio de cultivo com BAP para a proliferação in vitro de brotações de O. selloi.Sociedade Brasileira de Plantas Medicinais2019-12-19T17:12:45Z2019-12-19T17:12:45Z2012info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfMONFORT, L. E. F.; PINTO, J. E. P. B.; BERTOLUCCI, S. K. V.; ROSSI, Z. T. T.; SANTOS, F. M. Efeito do BAP no cultivo in vitro de Ocimum selloi Benth. Revista Brasileira de Plantas Medicinais, Botucatu, v. 14, n. 3, p. 458-463, 2012.http://repositorio.ufla.br/jspui/handle/1/38317Revista Brasileira de Plantas Medicinaisreponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessMonfort, L. E. F.Pinto, J. E. B. P.Bertolucci, S. K. V.Rossi, Z. T. T.Santos, F. M.por2023-05-26T18:51:18Zoai:localhost:1/38317Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2023-05-26T18:51:18Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false |
dc.title.none.fl_str_mv |
Efeito do BAP no cultivo in vitro de Ocimum selloi Benth Effect of BAP on in vitro culture of Ocimum selloi Benth |
title |
Efeito do BAP no cultivo in vitro de Ocimum selloi Benth |
spellingShingle |
Efeito do BAP no cultivo in vitro de Ocimum selloi Benth Monfort, L. E. F. Plantas medicinais Benzilaminopurina Atroveran - Micropropagation Medicinal plants Benzylaminopurine Atroveran - Micropropagação |
title_short |
Efeito do BAP no cultivo in vitro de Ocimum selloi Benth |
title_full |
Efeito do BAP no cultivo in vitro de Ocimum selloi Benth |
title_fullStr |
Efeito do BAP no cultivo in vitro de Ocimum selloi Benth |
title_full_unstemmed |
Efeito do BAP no cultivo in vitro de Ocimum selloi Benth |
title_sort |
Efeito do BAP no cultivo in vitro de Ocimum selloi Benth |
author |
Monfort, L. E. F. |
author_facet |
Monfort, L. E. F. Pinto, J. E. B. P. Bertolucci, S. K. V. Rossi, Z. T. T. Santos, F. M. |
author_role |
author |
author2 |
Pinto, J. E. B. P. Bertolucci, S. K. V. Rossi, Z. T. T. Santos, F. M. |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Monfort, L. E. F. Pinto, J. E. B. P. Bertolucci, S. K. V. Rossi, Z. T. T. Santos, F. M. |
dc.subject.por.fl_str_mv |
Plantas medicinais Benzilaminopurina Atroveran - Micropropagation Medicinal plants Benzylaminopurine Atroveran - Micropropagação |
topic |
Plantas medicinais Benzilaminopurina Atroveran - Micropropagation Medicinal plants Benzylaminopurine Atroveran - Micropropagação |
description |
The present study was undertaken to develop the proliferation of sprouts in apical and nodal segments of Ocimum selloi with different BAP levels. Young plants aged 60 days were used as donors of nodal and apical segments. The segments were inoculated in MS medium at half the concentration of salts supplemented with 1.5% of sucrose and different BAP levels. The experiment was in 3 x 4 factorial arrangement, 3 positions of O. selloi segments (apical segment; first and second nodal segment) and 3 BAP levels (0 - control; 2; 4 and 6 mg L-1). After 30 days, the number, the length, and the fresh and dry biomass of sprouts and roots were evaluated. The first and the second segments showed better results in inducing O. selloi sprouts, 7 and 8 sprouts/explant, at the different BAP levels, but there was not root formation in the presence of the cytokinin. Under the tested conditions, use of the first and the second nodal segments is recommended in addition to supplementing the culture medium with BAP for in vitro proliferation of O. selloi sprouts. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012 2019-12-19T17:12:45Z 2019-12-19T17:12:45Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
MONFORT, L. E. F.; PINTO, J. E. P. B.; BERTOLUCCI, S. K. V.; ROSSI, Z. T. T.; SANTOS, F. M. Efeito do BAP no cultivo in vitro de Ocimum selloi Benth. Revista Brasileira de Plantas Medicinais, Botucatu, v. 14, n. 3, p. 458-463, 2012. http://repositorio.ufla.br/jspui/handle/1/38317 |
identifier_str_mv |
MONFORT, L. E. F.; PINTO, J. E. P. B.; BERTOLUCCI, S. K. V.; ROSSI, Z. T. T.; SANTOS, F. M. Efeito do BAP no cultivo in vitro de Ocimum selloi Benth. Revista Brasileira de Plantas Medicinais, Botucatu, v. 14, n. 3, p. 458-463, 2012. |
url |
http://repositorio.ufla.br/jspui/handle/1/38317 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/ |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Plantas Medicinais |
publisher.none.fl_str_mv |
Sociedade Brasileira de Plantas Medicinais |
dc.source.none.fl_str_mv |
Revista Brasileira de Plantas Medicinais reponame:Repositório Institucional da UFLA instname:Universidade Federal de Lavras (UFLA) instacron:UFLA |
instname_str |
Universidade Federal de Lavras (UFLA) |
instacron_str |
UFLA |
institution |
UFLA |
reponame_str |
Repositório Institucional da UFLA |
collection |
Repositório Institucional da UFLA |
repository.name.fl_str_mv |
Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA) |
repository.mail.fl_str_mv |
nivaldo@ufla.br || repositorio.biblioteca@ufla.br |
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1807835124272726016 |