Establishment of growth medium and quantification of pollen grains and germination of pear tree cultivars

Detalhes bibliográficos
Autor(a) principal: Nogueira, Paulyene Vieira
Data de Publicação: 2016
Outros Autores: Coutinho, Givago, Pio, Rafael, Silva, Daniel Fernandes da, Zambon, Carolina Ruiz
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFLA
Texto Completo: http://repositorio.ufla.br/jspui/handle/1/32967
Resumo: To support breeding programs of pear tree, on the selection of cultivars to subtropical area in Brazil, the objective of this research was to adjust the growth medium basic to pollen grain germination. The pollen grains of 'D'Água' cultivar were spread on the surface of Petri dishes containing 20 ml of culture medium in accordance with the following sequential experiments: 1) ágar (4; 6; 8 and 10 g L-1) and pH (3,5; 4,5; 5,5 and 6,5); 2) sucrose (0; 30; 60 and 90 g L-1); 3) calcium nitrate (0; 200; 400 and 800 mg L-1); 4) boric acid (0; 400; 800 and 1200 mg L-1); 5) temperature of incubation (15; 20; 25 e 30 ºC) and 6) emission time of the pollen tube (0; 1; 2; 3; 4; 5 and 6 hours after inoculation). After incubation, the germination rate of pollen grains of nine pear cultivars ('Rocha', 'Abate Fetel', 'Packham's Triumph', 'Atago', 'Hosui', 'Primorosa', 'Triunfo', 'Seleta' and 'D'Água') was evaluated, and number of stamens, the number of pollen grains per anther and per flower. The protocol for germination of pollen grains of pear tree consist in the culture medium have to be solidified with 10 g L-1 ágar, being the pH measured to 5,2 , added with 90 g L-1 sucrose, 145 mg L-1 calcium nitrate and 700 mg L-1 boric acid, with incubation temperature of 23 ºC. The readings germination percentage should be performed after five hours of incubation. The pollen grain 'Rocha' cultivar showed higher germination percentage.
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spelling Establishment of growth medium and quantification of pollen grains and germination of pear tree cultivarsEstabelecimento de meio de cultura e quantificação da quantidade de grãos de pólen e germinação entre cultivares de pereirasPyrus communisPyrus pyrifoliaPear tree - BreedingGermination ratePollen grain germinationPereira - Melhoramento genéticoTaxa de germinaçãoGerminação de grãos de pólenTo support breeding programs of pear tree, on the selection of cultivars to subtropical area in Brazil, the objective of this research was to adjust the growth medium basic to pollen grain germination. The pollen grains of 'D'Água' cultivar were spread on the surface of Petri dishes containing 20 ml of culture medium in accordance with the following sequential experiments: 1) ágar (4; 6; 8 and 10 g L-1) and pH (3,5; 4,5; 5,5 and 6,5); 2) sucrose (0; 30; 60 and 90 g L-1); 3) calcium nitrate (0; 200; 400 and 800 mg L-1); 4) boric acid (0; 400; 800 and 1200 mg L-1); 5) temperature of incubation (15; 20; 25 e 30 ºC) and 6) emission time of the pollen tube (0; 1; 2; 3; 4; 5 and 6 hours after inoculation). After incubation, the germination rate of pollen grains of nine pear cultivars ('Rocha', 'Abate Fetel', 'Packham's Triumph', 'Atago', 'Hosui', 'Primorosa', 'Triunfo', 'Seleta' and 'D'Água') was evaluated, and number of stamens, the number of pollen grains per anther and per flower. The protocol for germination of pollen grains of pear tree consist in the culture medium have to be solidified with 10 g L-1 ágar, being the pH measured to 5,2 , added with 90 g L-1 sucrose, 145 mg L-1 calcium nitrate and 700 mg L-1 boric acid, with incubation temperature of 23 ºC. The readings germination percentage should be performed after five hours of incubation. The pollen grain 'Rocha' cultivar showed higher germination percentage.Visando dar suporte aos trabalhos de melhoramento da pereira, para seleção de cultivares para regiões subtropicais, objetivou-se ajustar o meio de cultura para a germinação de grãos de pólen. Os grãos de pólen da cultivar 'D'Água' foram espalhados sobre placas de Petri contendo 20 mL de meio, de acordo com os experimentos sequenciais: 1) ágar (4; 6; 8 e 10 g L-1) e pH (3,5; 4,5; 5,5 e 6,5); 2) sacarose (0; 30; 60 e 90 g L-1); 3) nitrato de cálcio (0; 200; 400 e 800 mg L-1); 4) ácido bórico (0; 400; 800 e 1200 mg L-1); 5) temperatura de incubação (15; 20; 25 e 30 ºC) e 6) tempo de emissão do tubo polínico (0; 1; 2; 3; 4; 5 e 6 horas após a inoculação). Após a inoculação avaliou-se a germinação dos grãos de pólen de nove cultivares ('Rocha', 'Abate Fetel', 'Packham's Triumph', 'Atago', 'Hosui', 'Primorosa', 'Triunfo', 'Seleta' e 'D'Água'), além do número de anteras, número de grãos de pólen por antera e por flor. O protocolo para germinação de grãos de pólen de pereira consiste no meio de cultura ser solidificado com 10 g L-1 de ágar, com pH ideal de 5,2, acrescido de 90 g L-1 de sacarose, 145 mg L-1 de nitrato de cálcio e 700 mg L-1 de ácido bórico, com temperatura de incubação de 23,7 ºC. As análises de germinação devem ser realizadas após 5h de incubação. Os grãos de pólen da cultivar 'Rocha' apresentaram maior porcentagem de germinação.Universidade Federal do Ceará2019-02-22T17:40:30Z2019-02-22T17:40:30Z2016-04info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfNOGUEIRA, P. V. et al. Establishment of growth medium and quantification of pollen grains and germination of pear tree cultivars. Revista Ciência Agronômica, Fortaleza, v. 47, n. 2, p. 380-386, abr./jun. 2016.http://repositorio.ufla.br/jspui/handle/1/32967Revista Ciência Agronômicareponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessNogueira, Paulyene VieiraCoutinho, GivagoPio, RafaelSilva, Daniel Fernandes daZambon, Carolina Ruizeng2023-05-26T18:43:50Zoai:localhost:1/32967Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2023-05-26T18:43:50Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false
dc.title.none.fl_str_mv Establishment of growth medium and quantification of pollen grains and germination of pear tree cultivars
Estabelecimento de meio de cultura e quantificação da quantidade de grãos de pólen e germinação entre cultivares de pereiras
title Establishment of growth medium and quantification of pollen grains and germination of pear tree cultivars
spellingShingle Establishment of growth medium and quantification of pollen grains and germination of pear tree cultivars
Nogueira, Paulyene Vieira
Pyrus communis
Pyrus pyrifolia
Pear tree - Breeding
Germination rate
Pollen grain germination
Pereira - Melhoramento genético
Taxa de germinação
Germinação de grãos de pólen
title_short Establishment of growth medium and quantification of pollen grains and germination of pear tree cultivars
title_full Establishment of growth medium and quantification of pollen grains and germination of pear tree cultivars
title_fullStr Establishment of growth medium and quantification of pollen grains and germination of pear tree cultivars
title_full_unstemmed Establishment of growth medium and quantification of pollen grains and germination of pear tree cultivars
title_sort Establishment of growth medium and quantification of pollen grains and germination of pear tree cultivars
author Nogueira, Paulyene Vieira
author_facet Nogueira, Paulyene Vieira
Coutinho, Givago
Pio, Rafael
Silva, Daniel Fernandes da
Zambon, Carolina Ruiz
author_role author
author2 Coutinho, Givago
Pio, Rafael
Silva, Daniel Fernandes da
Zambon, Carolina Ruiz
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Nogueira, Paulyene Vieira
Coutinho, Givago
Pio, Rafael
Silva, Daniel Fernandes da
Zambon, Carolina Ruiz
dc.subject.por.fl_str_mv Pyrus communis
Pyrus pyrifolia
Pear tree - Breeding
Germination rate
Pollen grain germination
Pereira - Melhoramento genético
Taxa de germinação
Germinação de grãos de pólen
topic Pyrus communis
Pyrus pyrifolia
Pear tree - Breeding
Germination rate
Pollen grain germination
Pereira - Melhoramento genético
Taxa de germinação
Germinação de grãos de pólen
description To support breeding programs of pear tree, on the selection of cultivars to subtropical area in Brazil, the objective of this research was to adjust the growth medium basic to pollen grain germination. The pollen grains of 'D'Água' cultivar were spread on the surface of Petri dishes containing 20 ml of culture medium in accordance with the following sequential experiments: 1) ágar (4; 6; 8 and 10 g L-1) and pH (3,5; 4,5; 5,5 and 6,5); 2) sucrose (0; 30; 60 and 90 g L-1); 3) calcium nitrate (0; 200; 400 and 800 mg L-1); 4) boric acid (0; 400; 800 and 1200 mg L-1); 5) temperature of incubation (15; 20; 25 e 30 ºC) and 6) emission time of the pollen tube (0; 1; 2; 3; 4; 5 and 6 hours after inoculation). After incubation, the germination rate of pollen grains of nine pear cultivars ('Rocha', 'Abate Fetel', 'Packham's Triumph', 'Atago', 'Hosui', 'Primorosa', 'Triunfo', 'Seleta' and 'D'Água') was evaluated, and number of stamens, the number of pollen grains per anther and per flower. The protocol for germination of pollen grains of pear tree consist in the culture medium have to be solidified with 10 g L-1 ágar, being the pH measured to 5,2 , added with 90 g L-1 sucrose, 145 mg L-1 calcium nitrate and 700 mg L-1 boric acid, with incubation temperature of 23 ºC. The readings germination percentage should be performed after five hours of incubation. The pollen grain 'Rocha' cultivar showed higher germination percentage.
publishDate 2016
dc.date.none.fl_str_mv 2016-04
2019-02-22T17:40:30Z
2019-02-22T17:40:30Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv NOGUEIRA, P. V. et al. Establishment of growth medium and quantification of pollen grains and germination of pear tree cultivars. Revista Ciência Agronômica, Fortaleza, v. 47, n. 2, p. 380-386, abr./jun. 2016.
http://repositorio.ufla.br/jspui/handle/1/32967
identifier_str_mv NOGUEIRA, P. V. et al. Establishment of growth medium and quantification of pollen grains and germination of pear tree cultivars. Revista Ciência Agronômica, Fortaleza, v. 47, n. 2, p. 380-386, abr./jun. 2016.
url http://repositorio.ufla.br/jspui/handle/1/32967
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal do Ceará
publisher.none.fl_str_mv Universidade Federal do Ceará
dc.source.none.fl_str_mv Revista Ciência Agronômica
reponame:Repositório Institucional da UFLA
instname:Universidade Federal de Lavras (UFLA)
instacron:UFLA
instname_str Universidade Federal de Lavras (UFLA)
instacron_str UFLA
institution UFLA
reponame_str Repositório Institucional da UFLA
collection Repositório Institucional da UFLA
repository.name.fl_str_mv Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)
repository.mail.fl_str_mv nivaldo@ufla.br || repositorio.biblioteca@ufla.br
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