Establishment of growth medium and quantification of pollen grains of olive cultivars in Brazil's subtropical areas

Detalhes bibliográficos
Autor(a) principal: Silva, Luiz Fernando de Oliveira da
Data de Publicação: 2016
Outros Autores: Zambon, Carolina Ruiz, Pio, Rafael, Oliveira, Adelson Francisco de, Gonçalves, Emerson Dias
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFLA
Texto Completo: http://repositorio.ufla.br/jspui/handle/1/32966
Resumo: Pollen grain germination in vitro indicates viability and consequently provides information related to fruit set. It also assists in the development of hybrids. Along with a suitable species, a standard culture medium is essential for evaluating pollen viability. It should contain a gelling agent consisting of carbohydrates and enhancer elements as well as have the correct pH, temperature, and incubation time. The objective of this study was to optimise the culture medium, determine the pollen germination capacity, and quantify the number of pollen grains per flower of certain olive tree cultivars. A basic sequential culture medium for pollen grain germination was determined, always utilizing the best result from the previous experiment to continue the sequence.The factorial treatment arrangement was: 1) agar versus boric acid; 2) pH versus sucrose; 3) calcium nitrate versus magnesium sulfate. After determining the culture medium components, two experiments were conducted evaluating temperature and incubation time. Another experiment evaluated both the germination percentage and the number of flower pollen grains of 28 cultivars. The culture medium should be composed of 4 g∙L-1 of agar, 90 g∙L-1 of sucrose, and 400 mg∙L-1 of boric acid with a pH adjusted to 5.79 and an incubation time of 60 h at 28 °C. The Manzanilla 215 cultivar had the highest germination rate while Ascolano 315 presented the highest number of pollen grains per flower.
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spelling Establishment of growth medium and quantification of pollen grains of olive cultivars in Brazil's subtropical areasOlea europaea L.Germination rateFruit setPollen grain germinationTaxa de germinaçãoGerminação de grãos de pólenPollen grain germination in vitro indicates viability and consequently provides information related to fruit set. It also assists in the development of hybrids. Along with a suitable species, a standard culture medium is essential for evaluating pollen viability. It should contain a gelling agent consisting of carbohydrates and enhancer elements as well as have the correct pH, temperature, and incubation time. The objective of this study was to optimise the culture medium, determine the pollen germination capacity, and quantify the number of pollen grains per flower of certain olive tree cultivars. A basic sequential culture medium for pollen grain germination was determined, always utilizing the best result from the previous experiment to continue the sequence.The factorial treatment arrangement was: 1) agar versus boric acid; 2) pH versus sucrose; 3) calcium nitrate versus magnesium sulfate. After determining the culture medium components, two experiments were conducted evaluating temperature and incubation time. Another experiment evaluated both the germination percentage and the number of flower pollen grains of 28 cultivars. The culture medium should be composed of 4 g∙L-1 of agar, 90 g∙L-1 of sucrose, and 400 mg∙L-1 of boric acid with a pH adjusted to 5.79 and an incubation time of 60 h at 28 °C. The Manzanilla 215 cultivar had the highest germination rate while Ascolano 315 presented the highest number of pollen grains per flower.Instituto Agronômico de Campinas2019-02-22T17:21:45Z2019-02-22T17:21:45Z2016-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfSILVA, L. F. de O. da et al. Establishment of growth medium and quantification of pollen grains of olive cultivars in Brazil's subtropical áreas. Bragantia, Campinas, v. 75, n. 1, p. 26-32, Jan./Mar. 2016.http://repositorio.ufla.br/jspui/handle/1/32966Bragantiareponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessSilva, Luiz Fernando de Oliveira daZambon, Carolina RuizPio, RafaelOliveira, Adelson Francisco deGonçalves, Emerson Diaseng2023-05-26T18:43:49Zoai:localhost:1/32966Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2023-05-26T18:43:49Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false
dc.title.none.fl_str_mv Establishment of growth medium and quantification of pollen grains of olive cultivars in Brazil's subtropical areas
title Establishment of growth medium and quantification of pollen grains of olive cultivars in Brazil's subtropical areas
spellingShingle Establishment of growth medium and quantification of pollen grains of olive cultivars in Brazil's subtropical areas
Silva, Luiz Fernando de Oliveira da
Olea europaea L.
Germination rate
Fruit set
Pollen grain germination
Taxa de germinação
Germinação de grãos de pólen
title_short Establishment of growth medium and quantification of pollen grains of olive cultivars in Brazil's subtropical areas
title_full Establishment of growth medium and quantification of pollen grains of olive cultivars in Brazil's subtropical areas
title_fullStr Establishment of growth medium and quantification of pollen grains of olive cultivars in Brazil's subtropical areas
title_full_unstemmed Establishment of growth medium and quantification of pollen grains of olive cultivars in Brazil's subtropical areas
title_sort Establishment of growth medium and quantification of pollen grains of olive cultivars in Brazil's subtropical areas
author Silva, Luiz Fernando de Oliveira da
author_facet Silva, Luiz Fernando de Oliveira da
Zambon, Carolina Ruiz
Pio, Rafael
Oliveira, Adelson Francisco de
Gonçalves, Emerson Dias
author_role author
author2 Zambon, Carolina Ruiz
Pio, Rafael
Oliveira, Adelson Francisco de
Gonçalves, Emerson Dias
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Silva, Luiz Fernando de Oliveira da
Zambon, Carolina Ruiz
Pio, Rafael
Oliveira, Adelson Francisco de
Gonçalves, Emerson Dias
dc.subject.por.fl_str_mv Olea europaea L.
Germination rate
Fruit set
Pollen grain germination
Taxa de germinação
Germinação de grãos de pólen
topic Olea europaea L.
Germination rate
Fruit set
Pollen grain germination
Taxa de germinação
Germinação de grãos de pólen
description Pollen grain germination in vitro indicates viability and consequently provides information related to fruit set. It also assists in the development of hybrids. Along with a suitable species, a standard culture medium is essential for evaluating pollen viability. It should contain a gelling agent consisting of carbohydrates and enhancer elements as well as have the correct pH, temperature, and incubation time. The objective of this study was to optimise the culture medium, determine the pollen germination capacity, and quantify the number of pollen grains per flower of certain olive tree cultivars. A basic sequential culture medium for pollen grain germination was determined, always utilizing the best result from the previous experiment to continue the sequence.The factorial treatment arrangement was: 1) agar versus boric acid; 2) pH versus sucrose; 3) calcium nitrate versus magnesium sulfate. After determining the culture medium components, two experiments were conducted evaluating temperature and incubation time. Another experiment evaluated both the germination percentage and the number of flower pollen grains of 28 cultivars. The culture medium should be composed of 4 g∙L-1 of agar, 90 g∙L-1 of sucrose, and 400 mg∙L-1 of boric acid with a pH adjusted to 5.79 and an incubation time of 60 h at 28 °C. The Manzanilla 215 cultivar had the highest germination rate while Ascolano 315 presented the highest number of pollen grains per flower.
publishDate 2016
dc.date.none.fl_str_mv 2016-01
2019-02-22T17:21:45Z
2019-02-22T17:21:45Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv SILVA, L. F. de O. da et al. Establishment of growth medium and quantification of pollen grains of olive cultivars in Brazil's subtropical áreas. Bragantia, Campinas, v. 75, n. 1, p. 26-32, Jan./Mar. 2016.
http://repositorio.ufla.br/jspui/handle/1/32966
identifier_str_mv SILVA, L. F. de O. da et al. Establishment of growth medium and quantification of pollen grains of olive cultivars in Brazil's subtropical áreas. Bragantia, Campinas, v. 75, n. 1, p. 26-32, Jan./Mar. 2016.
url http://repositorio.ufla.br/jspui/handle/1/32966
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Instituto Agronômico de Campinas
publisher.none.fl_str_mv Instituto Agronômico de Campinas
dc.source.none.fl_str_mv Bragantia
reponame:Repositório Institucional da UFLA
instname:Universidade Federal de Lavras (UFLA)
instacron:UFLA
instname_str Universidade Federal de Lavras (UFLA)
instacron_str UFLA
institution UFLA
reponame_str Repositório Institucional da UFLA
collection Repositório Institucional da UFLA
repository.name.fl_str_mv Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)
repository.mail.fl_str_mv nivaldo@ufla.br || repositorio.biblioteca@ufla.br
_version_ 1807835211612815360

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