Use of molecular markers SSR and SCAR for identification of olive accessions
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFLA |
Texto Completo: | http://repositorio.ufla.br/jspui/handle/1/43237 |
Resumo: | Morphological and agronomical describers are traditionally used in plant characterization. However, the usage of these describers have some limitations such as susceptibility to abiotic and biotic stress and environmental factors. Furthermore, the describers are not stable over time and many can only be evaluated during the adult phase of the plants, which requires time and physical space. Molecular markers offer numerous advantages compared to the conventional alternatives based on phenotype: they are stable and detectable in all vegetable tissues, and are independent of the environment and development phase. One of the main advantages of the use of molecular markers is the time reduction in the identification of genetic diversity among the studied subjects, as the genotypes may even be described for the seed or seedling phase. Many countries have already adopted molecular markers to identify olive cultivars more accurately. The aim of this study was to evaluate the genetic identity of eight olive accessions supposedly belonging to cultivar Arbequina by using microsatellite (SSR) and Sequence Characterized Amplified Region (SCAR) markers. One accession corresponding to the cultivar was also incorporated into the analysis as a reference genotype. The molecular marker data were analyzed on the software GENALEX6.The markers generated an accumulated PI and PE of 1.26 x 10-6 and 0.949, respectively.The results supported the hypothesis that all accessions belong to the cultivar Arbequina, and the markers can therefore be applied to other varieties of olive species. |
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Use of molecular markers SSR and SCAR for identification of olive accessionsUso dos marcadores moleculares SSR e SCAR para identificação de acessos de oliveiraOlea europaeaArbequinaDNA fingerprintingHomonymySynonymyMorphological and agronomical describers are traditionally used in plant characterization. However, the usage of these describers have some limitations such as susceptibility to abiotic and biotic stress and environmental factors. Furthermore, the describers are not stable over time and many can only be evaluated during the adult phase of the plants, which requires time and physical space. Molecular markers offer numerous advantages compared to the conventional alternatives based on phenotype: they are stable and detectable in all vegetable tissues, and are independent of the environment and development phase. One of the main advantages of the use of molecular markers is the time reduction in the identification of genetic diversity among the studied subjects, as the genotypes may even be described for the seed or seedling phase. Many countries have already adopted molecular markers to identify olive cultivars more accurately. The aim of this study was to evaluate the genetic identity of eight olive accessions supposedly belonging to cultivar Arbequina by using microsatellite (SSR) and Sequence Characterized Amplified Region (SCAR) markers. One accession corresponding to the cultivar was also incorporated into the analysis as a reference genotype. The molecular marker data were analyzed on the software GENALEX6.The markers generated an accumulated PI and PE of 1.26 x 10-6 and 0.949, respectively.The results supported the hypothesis that all accessions belong to the cultivar Arbequina, and the markers can therefore be applied to other varieties of olive species.Universidade Federal de Uberlândia2020-09-29T18:59:51Z2020-09-29T18:59:51Z2020info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfVAL, A. D.A B. do et al. Use of molecular markers SSR and SCAR for identification of olive accessions. Bioscience Journal, Uberlândia, v. 36, n. 4, p. 1137-1145, July/Aug. 2020.http://repositorio.ufla.br/jspui/handle/1/43237Bioscience Journalreponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessVal, Aurinete Daienn Borges doBreves, Sâmera de SouzaCançado, Geraldo Magela de AlmeidaFerreira, Juliano LinoPasqual, Moacireng2023-05-26T18:52:43Zoai:localhost:1/43237Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2023-05-26T18:52:43Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false |
dc.title.none.fl_str_mv |
Use of molecular markers SSR and SCAR for identification of olive accessions Uso dos marcadores moleculares SSR e SCAR para identificação de acessos de oliveira |
title |
Use of molecular markers SSR and SCAR for identification of olive accessions |
spellingShingle |
Use of molecular markers SSR and SCAR for identification of olive accessions Val, Aurinete Daienn Borges do Olea europaea Arbequina DNA fingerprinting Homonymy Synonymy |
title_short |
Use of molecular markers SSR and SCAR for identification of olive accessions |
title_full |
Use of molecular markers SSR and SCAR for identification of olive accessions |
title_fullStr |
Use of molecular markers SSR and SCAR for identification of olive accessions |
title_full_unstemmed |
Use of molecular markers SSR and SCAR for identification of olive accessions |
title_sort |
Use of molecular markers SSR and SCAR for identification of olive accessions |
author |
Val, Aurinete Daienn Borges do |
author_facet |
Val, Aurinete Daienn Borges do Breves, Sâmera de Souza Cançado, Geraldo Magela de Almeida Ferreira, Juliano Lino Pasqual, Moacir |
author_role |
author |
author2 |
Breves, Sâmera de Souza Cançado, Geraldo Magela de Almeida Ferreira, Juliano Lino Pasqual, Moacir |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Val, Aurinete Daienn Borges do Breves, Sâmera de Souza Cançado, Geraldo Magela de Almeida Ferreira, Juliano Lino Pasqual, Moacir |
dc.subject.por.fl_str_mv |
Olea europaea Arbequina DNA fingerprinting Homonymy Synonymy |
topic |
Olea europaea Arbequina DNA fingerprinting Homonymy Synonymy |
description |
Morphological and agronomical describers are traditionally used in plant characterization. However, the usage of these describers have some limitations such as susceptibility to abiotic and biotic stress and environmental factors. Furthermore, the describers are not stable over time and many can only be evaluated during the adult phase of the plants, which requires time and physical space. Molecular markers offer numerous advantages compared to the conventional alternatives based on phenotype: they are stable and detectable in all vegetable tissues, and are independent of the environment and development phase. One of the main advantages of the use of molecular markers is the time reduction in the identification of genetic diversity among the studied subjects, as the genotypes may even be described for the seed or seedling phase. Many countries have already adopted molecular markers to identify olive cultivars more accurately. The aim of this study was to evaluate the genetic identity of eight olive accessions supposedly belonging to cultivar Arbequina by using microsatellite (SSR) and Sequence Characterized Amplified Region (SCAR) markers. One accession corresponding to the cultivar was also incorporated into the analysis as a reference genotype. The molecular marker data were analyzed on the software GENALEX6.The markers generated an accumulated PI and PE of 1.26 x 10-6 and 0.949, respectively.The results supported the hypothesis that all accessions belong to the cultivar Arbequina, and the markers can therefore be applied to other varieties of olive species. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-09-29T18:59:51Z 2020-09-29T18:59:51Z 2020 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
VAL, A. D.A B. do et al. Use of molecular markers SSR and SCAR for identification of olive accessions. Bioscience Journal, Uberlândia, v. 36, n. 4, p. 1137-1145, July/Aug. 2020. http://repositorio.ufla.br/jspui/handle/1/43237 |
identifier_str_mv |
VAL, A. D.A B. do et al. Use of molecular markers SSR and SCAR for identification of olive accessions. Bioscience Journal, Uberlândia, v. 36, n. 4, p. 1137-1145, July/Aug. 2020. |
url |
http://repositorio.ufla.br/jspui/handle/1/43237 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/ |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Uberlândia |
publisher.none.fl_str_mv |
Universidade Federal de Uberlândia |
dc.source.none.fl_str_mv |
Bioscience Journal reponame:Repositório Institucional da UFLA instname:Universidade Federal de Lavras (UFLA) instacron:UFLA |
instname_str |
Universidade Federal de Lavras (UFLA) |
instacron_str |
UFLA |
institution |
UFLA |
reponame_str |
Repositório Institucional da UFLA |
collection |
Repositório Institucional da UFLA |
repository.name.fl_str_mv |
Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA) |
repository.mail.fl_str_mv |
nivaldo@ufla.br || repositorio.biblioteca@ufla.br |
_version_ |
1784550105591840768 |