Purification and biochemical characterization of an extracellular lipase produced by a new strain of Rhizopus sp.

Detalhes bibliográficos
Autor(a) principal: Koblitz, Maria Gabriela Bello
Data de Publicação: 2006
Outros Autores: Pastore, Gláucia Maria
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFLA
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542006000300016
http://repositorio.ufla.br/jspui/handle/1/6226
Resumo: The present study had as a goal to purify and characterize the lipolytic fraction secreted by a strain of Rhizopus sp. Only 3 steps of purification were necessary to achieve SDS-PAGE homogeneity. One band with 37.5 KDa molecular mass and with 1446 U/mg specific activity was obtained. The purified fraction presented 2 lipase isoforms; both showed optimum activity at 50ºC, and were stable between 6.5 and 7.5 pH values and at temperatures below 50ºC and also kept their activity in hexane. The lipase was inactivated by Hg+2 and by n-bromosuccinimide and activated by Na+.
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spelling Purification and biochemical characterization of an extracellular lipase produced by a new strain of Rhizopus sp.LipaseRhizopus sp.PurificationBiochemical characterizationThe present study had as a goal to purify and characterize the lipolytic fraction secreted by a strain of Rhizopus sp. Only 3 steps of purification were necessary to achieve SDS-PAGE homogeneity. One band with 37.5 KDa molecular mass and with 1446 U/mg specific activity was obtained. The purified fraction presented 2 lipase isoforms; both showed optimum activity at 50ºC, and were stable between 6.5 and 7.5 pH values and at temperatures below 50ºC and also kept their activity in hexane. The lipase was inactivated by Hg+2 and by n-bromosuccinimide and activated by Na+.Editora da Universidade Federal de Lavras2006-06-012015-04-30T13:33:47Z2015-04-30T13:33:47Z2015-04-30info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articletext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542006000300016KOBLITZ, M. G. B.; PASTORE, G. M. Purification and biochemical characterization of an extracellular lipase produced by a new strain of Rhizopus sp. Ciência e Agrotecnologia, Lavras, v. 30, n. 3 , p. 494-502, maio 2006.http://repositorio.ufla.br/jspui/handle/1/6226Ciência e Agrotecnologia v.30 n.3 2006reponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAKoblitz, Maria Gabriela BelloPastore, Gláucia Mariaenginfo:eu-repo/semantics/openAccess2016-09-27T13:26:47Zoai:localhost:1/6226Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2016-09-27T13:26:47Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false
dc.title.none.fl_str_mv Purification and biochemical characterization of an extracellular lipase produced by a new strain of Rhizopus sp.
title Purification and biochemical characterization of an extracellular lipase produced by a new strain of Rhizopus sp.
spellingShingle Purification and biochemical characterization of an extracellular lipase produced by a new strain of Rhizopus sp.
Koblitz, Maria Gabriela Bello
Lipase
Rhizopus sp.
Purification
Biochemical characterization
title_short Purification and biochemical characterization of an extracellular lipase produced by a new strain of Rhizopus sp.
title_full Purification and biochemical characterization of an extracellular lipase produced by a new strain of Rhizopus sp.
title_fullStr Purification and biochemical characterization of an extracellular lipase produced by a new strain of Rhizopus sp.
title_full_unstemmed Purification and biochemical characterization of an extracellular lipase produced by a new strain of Rhizopus sp.
title_sort Purification and biochemical characterization of an extracellular lipase produced by a new strain of Rhizopus sp.
author Koblitz, Maria Gabriela Bello
author_facet Koblitz, Maria Gabriela Bello
Pastore, Gláucia Maria
author_role author
author2 Pastore, Gláucia Maria
author2_role author
dc.contributor.author.fl_str_mv Koblitz, Maria Gabriela Bello
Pastore, Gláucia Maria
dc.subject.por.fl_str_mv Lipase
Rhizopus sp.
Purification
Biochemical characterization
topic Lipase
Rhizopus sp.
Purification
Biochemical characterization
description The present study had as a goal to purify and characterize the lipolytic fraction secreted by a strain of Rhizopus sp. Only 3 steps of purification were necessary to achieve SDS-PAGE homogeneity. One band with 37.5 KDa molecular mass and with 1446 U/mg specific activity was obtained. The purified fraction presented 2 lipase isoforms; both showed optimum activity at 50ºC, and were stable between 6.5 and 7.5 pH values and at temperatures below 50ºC and also kept their activity in hexane. The lipase was inactivated by Hg+2 and by n-bromosuccinimide and activated by Na+.
publishDate 2006
dc.date.none.fl_str_mv 2006-06-01
2015-04-30T13:33:47Z
2015-04-30T13:33:47Z
2015-04-30
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542006000300016
KOBLITZ, M. G. B.; PASTORE, G. M. Purification and biochemical characterization of an extracellular lipase produced by a new strain of Rhizopus sp. Ciência e Agrotecnologia, Lavras, v. 30, n. 3 , p. 494-502, maio 2006.
http://repositorio.ufla.br/jspui/handle/1/6226
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542006000300016
http://repositorio.ufla.br/jspui/handle/1/6226
identifier_str_mv KOBLITZ, M. G. B.; PASTORE, G. M. Purification and biochemical characterization of an extracellular lipase produced by a new strain of Rhizopus sp. Ciência e Agrotecnologia, Lavras, v. 30, n. 3 , p. 494-502, maio 2006.
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Editora da Universidade Federal de Lavras
publisher.none.fl_str_mv Editora da Universidade Federal de Lavras
dc.source.none.fl_str_mv Ciência e Agrotecnologia v.30 n.3 2006
reponame:Repositório Institucional da UFLA
instname:Universidade Federal de Lavras (UFLA)
instacron:UFLA
instname_str Universidade Federal de Lavras (UFLA)
instacron_str UFLA
institution UFLA
reponame_str Repositório Institucional da UFLA
collection Repositório Institucional da UFLA
repository.name.fl_str_mv Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)
repository.mail.fl_str_mv nivaldo@ufla.br || repositorio.biblioteca@ufla.br
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