Purification and biochemical characterization of an extracellular lipase produced by a new strain of Rhizopus sp.
Autor(a) principal: | |
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Data de Publicação: | 2006 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFLA |
Texto Completo: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542006000300016 http://repositorio.ufla.br/jspui/handle/1/6226 |
Resumo: | The present study had as a goal to purify and characterize the lipolytic fraction secreted by a strain of Rhizopus sp. Only 3 steps of purification were necessary to achieve SDS-PAGE homogeneity. One band with 37.5 KDa molecular mass and with 1446 U/mg specific activity was obtained. The purified fraction presented 2 lipase isoforms; both showed optimum activity at 50ºC, and were stable between 6.5 and 7.5 pH values and at temperatures below 50ºC and also kept their activity in hexane. The lipase was inactivated by Hg+2 and by n-bromosuccinimide and activated by Na+. |
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Purification and biochemical characterization of an extracellular lipase produced by a new strain of Rhizopus sp.LipaseRhizopus sp.PurificationBiochemical characterizationThe present study had as a goal to purify and characterize the lipolytic fraction secreted by a strain of Rhizopus sp. Only 3 steps of purification were necessary to achieve SDS-PAGE homogeneity. One band with 37.5 KDa molecular mass and with 1446 U/mg specific activity was obtained. The purified fraction presented 2 lipase isoforms; both showed optimum activity at 50ºC, and were stable between 6.5 and 7.5 pH values and at temperatures below 50ºC and also kept their activity in hexane. The lipase was inactivated by Hg+2 and by n-bromosuccinimide and activated by Na+.Editora da Universidade Federal de Lavras2006-06-012015-04-30T13:33:47Z2015-04-30T13:33:47Z2015-04-30info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articletext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542006000300016KOBLITZ, M. G. B.; PASTORE, G. M. Purification and biochemical characterization of an extracellular lipase produced by a new strain of Rhizopus sp. Ciência e Agrotecnologia, Lavras, v. 30, n. 3 , p. 494-502, maio 2006.http://repositorio.ufla.br/jspui/handle/1/6226Ciência e Agrotecnologia v.30 n.3 2006reponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAKoblitz, Maria Gabriela BelloPastore, Gláucia Mariaenginfo:eu-repo/semantics/openAccess2016-09-27T13:26:47Zoai:localhost:1/6226Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2016-09-27T13:26:47Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false |
dc.title.none.fl_str_mv |
Purification and biochemical characterization of an extracellular lipase produced by a new strain of Rhizopus sp. |
title |
Purification and biochemical characterization of an extracellular lipase produced by a new strain of Rhizopus sp. |
spellingShingle |
Purification and biochemical characterization of an extracellular lipase produced by a new strain of Rhizopus sp. Koblitz, Maria Gabriela Bello Lipase Rhizopus sp. Purification Biochemical characterization |
title_short |
Purification and biochemical characterization of an extracellular lipase produced by a new strain of Rhizopus sp. |
title_full |
Purification and biochemical characterization of an extracellular lipase produced by a new strain of Rhizopus sp. |
title_fullStr |
Purification and biochemical characterization of an extracellular lipase produced by a new strain of Rhizopus sp. |
title_full_unstemmed |
Purification and biochemical characterization of an extracellular lipase produced by a new strain of Rhizopus sp. |
title_sort |
Purification and biochemical characterization of an extracellular lipase produced by a new strain of Rhizopus sp. |
author |
Koblitz, Maria Gabriela Bello |
author_facet |
Koblitz, Maria Gabriela Bello Pastore, Gláucia Maria |
author_role |
author |
author2 |
Pastore, Gláucia Maria |
author2_role |
author |
dc.contributor.author.fl_str_mv |
Koblitz, Maria Gabriela Bello Pastore, Gláucia Maria |
dc.subject.por.fl_str_mv |
Lipase Rhizopus sp. Purification Biochemical characterization |
topic |
Lipase Rhizopus sp. Purification Biochemical characterization |
description |
The present study had as a goal to purify and characterize the lipolytic fraction secreted by a strain of Rhizopus sp. Only 3 steps of purification were necessary to achieve SDS-PAGE homogeneity. One band with 37.5 KDa molecular mass and with 1446 U/mg specific activity was obtained. The purified fraction presented 2 lipase isoforms; both showed optimum activity at 50ºC, and were stable between 6.5 and 7.5 pH values and at temperatures below 50ºC and also kept their activity in hexane. The lipase was inactivated by Hg+2 and by n-bromosuccinimide and activated by Na+. |
publishDate |
2006 |
dc.date.none.fl_str_mv |
2006-06-01 2015-04-30T13:33:47Z 2015-04-30T13:33:47Z 2015-04-30 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542006000300016 KOBLITZ, M. G. B.; PASTORE, G. M. Purification and biochemical characterization of an extracellular lipase produced by a new strain of Rhizopus sp. Ciência e Agrotecnologia, Lavras, v. 30, n. 3 , p. 494-502, maio 2006. http://repositorio.ufla.br/jspui/handle/1/6226 |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542006000300016 http://repositorio.ufla.br/jspui/handle/1/6226 |
identifier_str_mv |
KOBLITZ, M. G. B.; PASTORE, G. M. Purification and biochemical characterization of an extracellular lipase produced by a new strain of Rhizopus sp. Ciência e Agrotecnologia, Lavras, v. 30, n. 3 , p. 494-502, maio 2006. |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Editora da Universidade Federal de Lavras |
publisher.none.fl_str_mv |
Editora da Universidade Federal de Lavras |
dc.source.none.fl_str_mv |
Ciência e Agrotecnologia v.30 n.3 2006 reponame:Repositório Institucional da UFLA instname:Universidade Federal de Lavras (UFLA) instacron:UFLA |
instname_str |
Universidade Federal de Lavras (UFLA) |
instacron_str |
UFLA |
institution |
UFLA |
reponame_str |
Repositório Institucional da UFLA |
collection |
Repositório Institucional da UFLA |
repository.name.fl_str_mv |
Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA) |
repository.mail.fl_str_mv |
nivaldo@ufla.br || repositorio.biblioteca@ufla.br |
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1815439255184343040 |