Purification and biochemical characterization of an extracellular lipase produced by a new strain of Rhizopus sp.
Autor(a) principal: | |
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Data de Publicação: | 2006 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Ciência e Agrotecnologia (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542006000300016 |
Resumo: | The present study had as a goal to purify and characterize the lipolytic fraction secreted by a strain of Rhizopus sp. Only 3 steps of purification were necessary to achieve SDS-PAGE homogeneity. One band with 37.5 KDa molecular mass and with 1446 U/mg specific activity was obtained. The purified fraction presented 2 lipase isoforms; both showed optimum activity at 50ºC, and were stable between 6.5 and 7.5 pH values and at temperatures below 50ºC and also kept their activity in hexane. The lipase was inactivated by Hg+2 and by n-bromosuccinimide and activated by Na+. |
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Ciência e Agrotecnologia (Online) |
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Purification and biochemical characterization of an extracellular lipase produced by a new strain of Rhizopus sp.LipaseRhizopus sp.purificationbiochemical characterizationThe present study had as a goal to purify and characterize the lipolytic fraction secreted by a strain of Rhizopus sp. Only 3 steps of purification were necessary to achieve SDS-PAGE homogeneity. One band with 37.5 KDa molecular mass and with 1446 U/mg specific activity was obtained. The purified fraction presented 2 lipase isoforms; both showed optimum activity at 50ºC, and were stable between 6.5 and 7.5 pH values and at temperatures below 50ºC and also kept their activity in hexane. The lipase was inactivated by Hg+2 and by n-bromosuccinimide and activated by Na+.Editora da UFLA2006-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542006000300016Ciência e Agrotecnologia v.30 n.3 2006reponame:Ciência e Agrotecnologia (Online)instname:Universidade Federal de Lavras (UFLA)instacron:UFLA10.1590/S1413-70542006000300016info:eu-repo/semantics/openAccessKoblitz,Maria Gabriela BelloPastore,Gláucia Mariaeng2007-12-13T00:00:00Zoai:scielo:S1413-70542006000300016Revistahttp://www.scielo.br/cagroPUBhttps://old.scielo.br/oai/scielo-oai.php||renpaiva@dbi.ufla.br|| editora@editora.ufla.br1981-18291413-7054opendoar:2022-11-22T16:29:53.286174Ciência e Agrotecnologia (Online) - Universidade Federal de Lavras (UFLA)true |
dc.title.none.fl_str_mv |
Purification and biochemical characterization of an extracellular lipase produced by a new strain of Rhizopus sp. |
title |
Purification and biochemical characterization of an extracellular lipase produced by a new strain of Rhizopus sp. |
spellingShingle |
Purification and biochemical characterization of an extracellular lipase produced by a new strain of Rhizopus sp. Koblitz,Maria Gabriela Bello Lipase Rhizopus sp. purification biochemical characterization |
title_short |
Purification and biochemical characterization of an extracellular lipase produced by a new strain of Rhizopus sp. |
title_full |
Purification and biochemical characterization of an extracellular lipase produced by a new strain of Rhizopus sp. |
title_fullStr |
Purification and biochemical characterization of an extracellular lipase produced by a new strain of Rhizopus sp. |
title_full_unstemmed |
Purification and biochemical characterization of an extracellular lipase produced by a new strain of Rhizopus sp. |
title_sort |
Purification and biochemical characterization of an extracellular lipase produced by a new strain of Rhizopus sp. |
author |
Koblitz,Maria Gabriela Bello |
author_facet |
Koblitz,Maria Gabriela Bello Pastore,Gláucia Maria |
author_role |
author |
author2 |
Pastore,Gláucia Maria |
author2_role |
author |
dc.contributor.author.fl_str_mv |
Koblitz,Maria Gabriela Bello Pastore,Gláucia Maria |
dc.subject.por.fl_str_mv |
Lipase Rhizopus sp. purification biochemical characterization |
topic |
Lipase Rhizopus sp. purification biochemical characterization |
description |
The present study had as a goal to purify and characterize the lipolytic fraction secreted by a strain of Rhizopus sp. Only 3 steps of purification were necessary to achieve SDS-PAGE homogeneity. One band with 37.5 KDa molecular mass and with 1446 U/mg specific activity was obtained. The purified fraction presented 2 lipase isoforms; both showed optimum activity at 50ºC, and were stable between 6.5 and 7.5 pH values and at temperatures below 50ºC and also kept their activity in hexane. The lipase was inactivated by Hg+2 and by n-bromosuccinimide and activated by Na+. |
publishDate |
2006 |
dc.date.none.fl_str_mv |
2006-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542006000300016 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542006000300016 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1413-70542006000300016 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Editora da UFLA |
publisher.none.fl_str_mv |
Editora da UFLA |
dc.source.none.fl_str_mv |
Ciência e Agrotecnologia v.30 n.3 2006 reponame:Ciência e Agrotecnologia (Online) instname:Universidade Federal de Lavras (UFLA) instacron:UFLA |
instname_str |
Universidade Federal de Lavras (UFLA) |
instacron_str |
UFLA |
institution |
UFLA |
reponame_str |
Ciência e Agrotecnologia (Online) |
collection |
Ciência e Agrotecnologia (Online) |
repository.name.fl_str_mv |
Ciência e Agrotecnologia (Online) - Universidade Federal de Lavras (UFLA) |
repository.mail.fl_str_mv |
||renpaiva@dbi.ufla.br|| editora@editora.ufla.br |
_version_ |
1799874962708758528 |