Detecção de Wolbachia em mosquitos (Diptera: Culicidae) no estado do Maranhão, Brasil
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UFMA |
Texto Completo: | https://tedebc.ufma.br/jspui/handle/tede/tede/3379 |
Resumo: | It is estimated that about 66% of all known arthropod species are infected with Wolbachia. This endosymbiont has been widely studied due to its high natural diversity and mainly due to its ability to reduce infection by pathogens that cause diseases such as Dengue, Zika, Chikungunya, Yellow Fever and Malaria, transmitted by mosquitoes. Despite this, there is little information about the dispersion of Wolbachia in natural environments in Brazil. Such knowledge is important, as it allows estimating the extent of natural infection in hosts and the use of certain strains in the biological control of vector mosquitoes. The aim of this study was to evaluate the rate of natural infection by Wolbachia in different species of Culicidae in representative areas of the State of Maranhão. Mosquitoes were collected with CDC-HP traps, ovitraps, sapucaias and Shannon's tent in the municipalities of São Luís, São José de Ribamar, Chapadinha, Vitória do Mearim, Godofredo Viana and Pastos Bons. Insect DNA was extracted and the Wsp gene amplified by Polymerase Chain Reaction. In total, 448 individuals of 18 species and 7 genera of Culicidae were analyzed. Mosquitoes in Maranhão are infected with Wolbachia, with high frequencies of infection for physiologically more permissive species such as Ae. albopictus and Cx. quinquefasciatus. Overall, the average infection rate was 17%, with variation between species ranging from 4% to 80%. Wolbachia DNA was found in 6 species: Ae. albopictus, Ae. scapulari, Cx. quinquefasciatus, Cq. juxtamansonia and Cq. Venezuelensis. In addition to mosquitoes of the Anopheles (Nys.) spp and Cx. (Mel.) spp. complexes, this is the first record of Ae. scapulari, Cx. juxtamansonia, Cq. Venezuelensis infected with Wolbachia in the World. Species Ae. albopictus and Cx. quinquefasciatus for having a high prevalence and are the most suitable for studies aiming to assess the influence of Wolbachia on the life cycle and evolutionary processes of speciation. The average positivity in the genera Aedes (27%), Anopheles (2.5%), Culex (38%) and Coquillettidia (23%) allowed us to infer that Aedes, Culex and Coquillettidia have a high capacity to maintain fixation and endosymbiosis relationships in natural environments. Despite this, the infection was not detected in mosquitoes of the species Ae. Aegypti. Despite the low rate of infection in the genus Anopheles, here we detected infection in Anophelines of the subgenus Nyssorhynchus. Like Anopheles, Cellia and Lophopodomyia, Nyssorhynchus is physiologically permissive to support Wolbachia infection. |
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REBELO, José Manuel Macáriohttp://lattes.cnpq.br/1026308157852394REBELO, José Manuel Macáriohttp://lattes.cnpq.br/1026308157852394SILVA, Francinaldo Soareshttp://lattes.cnpq.br/3390673784617972SANTOS, Ciro Libio Caldas doshttp://lattes.cnpq.br/9415902500499227PINHEIRO, Valéria Cristina Soareshttp://lattes.cnpq.br/5443198431811220http://lattes.cnpq.br/7563155033410029CHAVES, Erick Barros2021-10-21T14:03:51Z2021-07-01CHAVES, Erick Barros. Detecção de Wolbachia em mosquitos (Diptera: Culicidae) no estado do Maranhão, Brasil. 2021. 57 f. Dissertação (Programa de Pós-Graduação em Ciências da Saúde/CCBS) - Universidade Federal do Maranhão, São Luís, 2021.https://tedebc.ufma.br/jspui/handle/tede/tede/3379It is estimated that about 66% of all known arthropod species are infected with Wolbachia. This endosymbiont has been widely studied due to its high natural diversity and mainly due to its ability to reduce infection by pathogens that cause diseases such as Dengue, Zika, Chikungunya, Yellow Fever and Malaria, transmitted by mosquitoes. Despite this, there is little information about the dispersion of Wolbachia in natural environments in Brazil. Such knowledge is important, as it allows estimating the extent of natural infection in hosts and the use of certain strains in the biological control of vector mosquitoes. The aim of this study was to evaluate the rate of natural infection by Wolbachia in different species of Culicidae in representative areas of the State of Maranhão. Mosquitoes were collected with CDC-HP traps, ovitraps, sapucaias and Shannon's tent in the municipalities of São Luís, São José de Ribamar, Chapadinha, Vitória do Mearim, Godofredo Viana and Pastos Bons. Insect DNA was extracted and the Wsp gene amplified by Polymerase Chain Reaction. In total, 448 individuals of 18 species and 7 genera of Culicidae were analyzed. Mosquitoes in Maranhão are infected with Wolbachia, with high frequencies of infection for physiologically more permissive species such as Ae. albopictus and Cx. quinquefasciatus. Overall, the average infection rate was 17%, with variation between species ranging from 4% to 80%. Wolbachia DNA was found in 6 species: Ae. albopictus, Ae. scapulari, Cx. quinquefasciatus, Cq. juxtamansonia and Cq. Venezuelensis. In addition to mosquitoes of the Anopheles (Nys.) spp and Cx. (Mel.) spp. complexes, this is the first record of Ae. scapulari, Cx. juxtamansonia, Cq. Venezuelensis infected with Wolbachia in the World. Species Ae. albopictus and Cx. quinquefasciatus for having a high prevalence and are the most suitable for studies aiming to assess the influence of Wolbachia on the life cycle and evolutionary processes of speciation. The average positivity in the genera Aedes (27%), Anopheles (2.5%), Culex (38%) and Coquillettidia (23%) allowed us to infer that Aedes, Culex and Coquillettidia have a high capacity to maintain fixation and endosymbiosis relationships in natural environments. Despite this, the infection was not detected in mosquitoes of the species Ae. Aegypti. Despite the low rate of infection in the genus Anopheles, here we detected infection in Anophelines of the subgenus Nyssorhynchus. Like Anopheles, Cellia and Lophopodomyia, Nyssorhynchus is physiologically permissive to support Wolbachia infection.Estima-se que cerca 66% de todas as espécies, de artrópodes conhecidas, estejam infectadas com Wolbachia. Esse endossimbionte vem sendo amplamente estudado devido a sua alta diversidade natural e principalmente graças a sua capacidade de reduzir a infecção por patógenos que causam doenças como Dengue, Zika, Chikungunya, Febre Amarela e Malária, transmitidas por mosquitos. Apesar disso, há poucas informações sobre a dispersão da Wolbachia em ambientes naturais no Brasil. O objetivo deste estudo foi avaliar a taxa de infecção natural por Wolbachia em diferentes espécies de culicídeos em áreas representativas do Estado do Maranhão. Os mosquitos foram coletados com armadilhas CDC-HP, ovitrampas, sapucaias e barraca de Shannon nos municípios de São Luís, São José de Ribamar, Chapadinha, Vitória do Mearim, Godofredo Viana e Pastos Bons. O DNA dos insetos foi extraído e amplificado o gene Wsp por Reação em Cadeia da Polimerase. No total, 448 indivíduos de 18 espécies e 7 gêneros de culicídeos foram analisados. Os mosquitos do Maranhão estão infectados coma Wolbachia, tendo altas frequencias de infecção para espécies fisiologicamente mais permissivas como Ae. albopictus e Cx. quinquefasciatus. No geral a taxa de infecção média foi 17%, com variação entre as espécies de 4% a 80%. O DNA de Wolbachia foi encontrado em 6 espécies: Ae. albopictus, Ae. scapulari, Cx. quinquefasciatus, Cq. juxtamansonia e Cq. venezuelensis. Além de mosquitos dos subgêneros Anopheles Nyssorhynchus e Culex. (Melanoconion). Esse é o primeiro registro de Ae. scapularis, Cx. juxtamansonia, Cq. venezuelensis infectados com a Wolbachia no Mundo. As espécies Ae. albopictus e Cx. quinquefasciatus por possuir alta prevalência e são as mais indicadas para estudos com objetivo de avaliar a influência da Wolbachia no ciclo de vida e processos evolutivos de especiação. A positividade média nos gêneros Aedes (27%), Anopheles (2,5%), Culex (38%) e Coquillettidia (23%) permitiu inferir que Aedes, Culex e Coquillettidia possuem alta capacidade manter relações de fixação e endossimbiose em ambientes naturais. Apesar disso, não detectou-se a infecção em mosquitos da espécie Ae. aegypti. Apesar da baixa taxa de infecção no gênero Anopheles aqui detectamos a infecção em Anofelinos dos subgêneros Nyssorhynchus. Assim como Anopheles, Cellia e Lophopodomyia o subgênero Nyssorhynchus é fisiologicamente permissivos para suportar a infecção por Wolbachia.Submitted by Sheila MONTEIRO (sheila.monteiro@ufma.br) on 2021-10-21T14:03:51Z No. of bitstreams: 1 ERICK-CHAVES.pdf: 931526 bytes, checksum: ded38d0de609cd64762756d6d098bf6e (MD5)Made available in DSpace on 2021-10-21T14:03:51Z (GMT). No. of bitstreams: 1 ERICK-CHAVES.pdf: 931526 bytes, checksum: ded38d0de609cd64762756d6d098bf6e (MD5) Previous issue date: 2021-07-01Fundação de Amparo à Pesquisa e ao Desenvolvimento Científico e Tecnológico do Maranhão - FAPEMACoordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESapplication/pdfporUniversidade Federal do MaranhãoPROGRAMA DE PÓS-GRADUAÇÃO EM CIÊNCIAS DA SAÚDE/CCBSUFMABrasilDEPARTAMENTO DE BIOLOGIA/CCBSInfecção NaturalPCRWolbachiaMaranhãoNatural InfectionMosquitoesWolbachiaMaranhãoCiências da SaúdeDetecção de Wolbachia em mosquitos (Diptera: Culicidae) no estado do Maranhão, BrasilDetection of Wolbachia in mosquitoes (Diptera: Culicidae) in the state of Maranhão, Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UFMAinstname:Universidade Federal do Maranhão (UFMA)instacron:UFMAORIGINALERICK-CHAVES.pdfERICK-CHAVES.pdfapplication/pdf931526http://tedebc.ufma.br:8080/bitstream/tede/3379/2/ERICK-CHAVES.pdfded38d0de609cd64762756d6d098bf6eMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82255http://tedebc.ufma.br:8080/bitstream/tede/3379/1/license.txt97eeade1fce43278e63fe063657f8083MD51tede/33792021-10-21 11:03:51.058oai:tede2:tede/3379IExJQ0VOw4dBIERFIERJU1RSSUJVScOHw4NPIE7Dg08tRVhDTFVTSVZBCgpDb20gYSBhcHJlc2VudGHDp8OjbyBkZXN0YSBsaWNlbsOnYSxvIGF1dG9yIChlcykgb3UgbyB0aXR1bGFyIGRvcyBkaXJlaXRvcyBkZSBhdXRvciBjb25jZWRlIMOgIFVuaXZlcnNpZGFkZSBGZWRlcmFsIGRvIE1hcmFuaMOjbyAoVUZNQSkgbyBkaXJlaXRvIG7Do28tZXhjbHVzaXZvIGRlIHJlcHJvZHV6aXIsIHRyYWR1emlyIChjb25mb3JtZSBkZWZpbmlkbyBhYmFpeG8pLCBlL291IGRpc3RyaWJ1aXIgYSBzdWEgdGVzZSBvdSBkaXNzZXJ0YcOnw6NvIChpbmNsdWluZG8gbyByZXN1bW8pIHBvciB0b2RvIG8gbXVuZG8gbm8gZm9ybWF0byBpbXByZXNzbyBlIGVsZXRyw7RuaWNvIGUgZW0gcXVhbHF1ZXIgbWVpbywgaW5jbHVpbmRvIG9zIGZvcm1hdG9zIMOhdWRpbyBvdSB2w61kZW8uCgpWb2PDqiBjb25jb3JkYSBxdWUgYSBVRk1BIHBvZGUsIHNlbSBhbHRlcmFyIG8gY29udGXDumRvLCB0cmFuc3BvciBhIHN1YSB0ZXNlIG91IGRpc3NlcnRhw6fDo28gcGFyYSBxdWFscXVlciBtZWlvIG91IGZvcm1hdG8gcGFyYSBmaW5zIGRlIHByZXNlcnZhw6fDo28uCgpWb2PDqiB0YW1iw6ltIGNvbmNvcmRhIHF1ZSBhIFVGTUEgcG9kZSBtYW50ZXIgbWFpcyBkZSB1bWEgY8OzcGlhIGRlIHN1YSB0ZXNlIG91IGRpc3NlcnRhw6fDo28gcGFyYSBmaW5zIGRlIHNlZ3VyYW7Dp2EsIGJhY2stdXAgZSBwcmVzZXJ2YcOnw6NvLgoKVm9jw6ogZGVjbGFyYSBxdWUgYSBzdWEgdGVzZSBvdSBkaXNzZXJ0YcOnw6NvIMOpIG9yaWdpbmFsIGUgcXVlIHZvY8OqIHRlbSBvIHBvZGVyIGRlIGNvbmNlZGVyIG9zIGRpcmVpdG9zIGNvbnRpZG9zIG5lc3RhIGxpY2Vuw6dhLiBWb2PDqiB0YW1iw6ltIGRlY2xhcmEgcXVlIG8gZGVww7NzaXRvIGRhIHN1YSB0ZXNlIG91IGRpc3NlcnRhw6fDo28gbsOjbywgcXVlIHNlamEgZGUgc2V1IGNvbmhlY2ltZW50bywgaW5mcmluZ2UgZGlyZWl0b3MgYXV0b3JhaXMgZGUgbmluZ3XDqW0uCgpDYXNvIGEgc3VhIHRlc2Ugb3UgZGlzc2VydGHDp8OjbyBjb250ZW5oYSBtYXRlcmlhbCBxdWUgdm9jw6ogbsOjbyBwb3NzdWkgYSB0aXR1bGFyaWRhZGUgZG9zIGRpcmVpdG9zIGF1dG9yYWlzLCB2b2PDqiBkZWNsYXJhIHF1ZSBvYnRldmUgYSBwZXJtaXNzw6NvIGlycmVzdHJpdGEgZG8gZGV0ZW50b3IgZG9zIGRpcmVpdG9zIGF1dG9yYWlzIHBhcmEgY29uY2VkZXIgw6AgVUZNQSBvcyBkaXJlaXRvcyBhcHJlc2VudGFkb3MgbmVzdGEgbGljZW7Dp2EsIGUgcXVlIGVzc2UgbWF0ZXJpYWwgZGUgcHJvcHJpZWRhZGUgZGUgdGVyY2Vpcm9zIGVzdMOhIGNsYXJhbWVudGUgaWRlbnRpZmljYWRvIGUgcmVjb25oZWNpZG8gbm8gdGV4dG8gb3Ugbm8gY29udGXDumRvIGRhIHRlc2Ugb3UgZGlzc2VydGHDp8OjbyBvcmEgZGVwb3NpdGFkYS4KCkNBU08gQSBURVNFIE9VIERJU1NFUlRBw4fDg08gT1JBIERFUE9TSVRBREEgVEVOSEEgU0lETyBSRVNVTFRBRE8gREUgVU0gUEFUUk9Dw41OSU8gT1UgQVBPSU8gREUgVU1BIEFHw4pOQ0lBIERFIEZPTUVOVE8gT1UgT1VUUk8gT1JHQU5JU01PIFFVRSBOw4NPIFNFSkEgQSBVRk1BLCBWT0PDiiBERUNMQVJBIFFVRSBSRVNQRUlUT1UgVE9ET1MgRSBRVUFJU1FVRVIgRElSRUlUT1MgREUgUkVWSVPDg08gQ09NTyBUQU1Cw4lNIEFTIERFTUFJUyBPQlJJR0HDh8OVRVMgRVhJR0lEQVMgUE9SIENPTlRSQVRPIE9VIEFDT1JETy4KCkEgVUZNQSBzZSBjb21wcm9tZXRlIGEgaWRlbnRpZmljYXIgY2xhcmFtZW50ZSBvIHNldSBub21lIG91IG8ocykgbm9tZShzKSBkbyhzKSBkZXRlbnRvcihlcykgZG9zIGRpcmVpdG9zIGF1dG9yYWlzIGRhIHRlc2Ugb3UgZGlzc2VydGHDp8OjbywgZSBuw6NvIGZhcsOhIHF1YWxxdWVyIGFsdGVyYcOnw6NvLCBhbMOpbSBkYXF1ZWxhcyBjb25jZWRpZGFzIHBvciBlc3RhIGxpY2Vuw6dhLgoKRGVjbGFyYSB0YW1iw6ltIHF1ZSB0b2RhcyBhcyBhZmlsaWHDp8O1ZXMgY29ycG9yYXRpdmFzIG91IGluc3RpdHVjaW9uYWlzIGUgdG9kYXMgYXMgZm9udGVzIGRlIGFwb2lvIGZpbmFuY2Vpcm8gYW8gdHJhYmFsaG8gZXN0w6NvIGRldmlkYW1lbnRlIGNpdGFkYXMgb3UgbWVuY2lvbmFkYXMgZSBjZXJ0aWZpY2EgcXVlIG7Do28gaMOhIG5lbmh1bSBpbnRlcmVzc2UgY29tZXJjaWFsIG91IGFzc29jaWF0aXZvIHF1ZSByZXByZXNlbnRlIGNvbmZsaXRvIGRlIGludGVyZXNzZSBlbSBjb25leMOjbyBjb20gbyB0cmFiYWxobyBzdWJtZXRpZG8uCgoKCgoKCgo=Biblioteca Digital de Teses e Dissertaçõeshttps://tedebc.ufma.br/jspui/PUBhttp://tedebc.ufma.br:8080/oai/requestrepositorio@ufma.br||repositorio@ufma.bropendoar:21312021-10-21T14:03:51Biblioteca Digital de Teses e Dissertações da UFMA - Universidade Federal do Maranhão (UFMA)false |
dc.title.por.fl_str_mv |
Detecção de Wolbachia em mosquitos (Diptera: Culicidae) no estado do Maranhão, Brasil |
dc.title.alternative.eng.fl_str_mv |
Detection of Wolbachia in mosquitoes (Diptera: Culicidae) in the state of Maranhão, Brazil |
title |
Detecção de Wolbachia em mosquitos (Diptera: Culicidae) no estado do Maranhão, Brasil |
spellingShingle |
Detecção de Wolbachia em mosquitos (Diptera: Culicidae) no estado do Maranhão, Brasil CHAVES, Erick Barros Infecção Natural PCR Wolbachia Maranhão Natural Infection Mosquitoes Wolbachia Maranhão Ciências da Saúde |
title_short |
Detecção de Wolbachia em mosquitos (Diptera: Culicidae) no estado do Maranhão, Brasil |
title_full |
Detecção de Wolbachia em mosquitos (Diptera: Culicidae) no estado do Maranhão, Brasil |
title_fullStr |
Detecção de Wolbachia em mosquitos (Diptera: Culicidae) no estado do Maranhão, Brasil |
title_full_unstemmed |
Detecção de Wolbachia em mosquitos (Diptera: Culicidae) no estado do Maranhão, Brasil |
title_sort |
Detecção de Wolbachia em mosquitos (Diptera: Culicidae) no estado do Maranhão, Brasil |
author |
CHAVES, Erick Barros |
author_facet |
CHAVES, Erick Barros |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
REBELO, José Manuel Macário |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/1026308157852394 |
dc.contributor.referee1.fl_str_mv |
REBELO, José Manuel Macário |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/1026308157852394 |
dc.contributor.referee2.fl_str_mv |
SILVA, Francinaldo Soares |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/3390673784617972 |
dc.contributor.referee3.fl_str_mv |
SANTOS, Ciro Libio Caldas dos |
dc.contributor.referee3Lattes.fl_str_mv |
http://lattes.cnpq.br/9415902500499227 |
dc.contributor.referee4.fl_str_mv |
PINHEIRO, Valéria Cristina Soares |
dc.contributor.referee4Lattes.fl_str_mv |
http://lattes.cnpq.br/5443198431811220 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/7563155033410029 |
dc.contributor.author.fl_str_mv |
CHAVES, Erick Barros |
contributor_str_mv |
REBELO, José Manuel Macário REBELO, José Manuel Macário SILVA, Francinaldo Soares SANTOS, Ciro Libio Caldas dos PINHEIRO, Valéria Cristina Soares |
dc.subject.por.fl_str_mv |
Infecção Natural PCR Wolbachia Maranhão |
topic |
Infecção Natural PCR Wolbachia Maranhão Natural Infection Mosquitoes Wolbachia Maranhão Ciências da Saúde |
dc.subject.eng.fl_str_mv |
Natural Infection Mosquitoes Wolbachia Maranhão |
dc.subject.cnpq.fl_str_mv |
Ciências da Saúde |
description |
It is estimated that about 66% of all known arthropod species are infected with Wolbachia. This endosymbiont has been widely studied due to its high natural diversity and mainly due to its ability to reduce infection by pathogens that cause diseases such as Dengue, Zika, Chikungunya, Yellow Fever and Malaria, transmitted by mosquitoes. Despite this, there is little information about the dispersion of Wolbachia in natural environments in Brazil. Such knowledge is important, as it allows estimating the extent of natural infection in hosts and the use of certain strains in the biological control of vector mosquitoes. The aim of this study was to evaluate the rate of natural infection by Wolbachia in different species of Culicidae in representative areas of the State of Maranhão. Mosquitoes were collected with CDC-HP traps, ovitraps, sapucaias and Shannon's tent in the municipalities of São Luís, São José de Ribamar, Chapadinha, Vitória do Mearim, Godofredo Viana and Pastos Bons. Insect DNA was extracted and the Wsp gene amplified by Polymerase Chain Reaction. In total, 448 individuals of 18 species and 7 genera of Culicidae were analyzed. Mosquitoes in Maranhão are infected with Wolbachia, with high frequencies of infection for physiologically more permissive species such as Ae. albopictus and Cx. quinquefasciatus. Overall, the average infection rate was 17%, with variation between species ranging from 4% to 80%. Wolbachia DNA was found in 6 species: Ae. albopictus, Ae. scapulari, Cx. quinquefasciatus, Cq. juxtamansonia and Cq. Venezuelensis. In addition to mosquitoes of the Anopheles (Nys.) spp and Cx. (Mel.) spp. complexes, this is the first record of Ae. scapulari, Cx. juxtamansonia, Cq. Venezuelensis infected with Wolbachia in the World. Species Ae. albopictus and Cx. quinquefasciatus for having a high prevalence and are the most suitable for studies aiming to assess the influence of Wolbachia on the life cycle and evolutionary processes of speciation. The average positivity in the genera Aedes (27%), Anopheles (2.5%), Culex (38%) and Coquillettidia (23%) allowed us to infer that Aedes, Culex and Coquillettidia have a high capacity to maintain fixation and endosymbiosis relationships in natural environments. Despite this, the infection was not detected in mosquitoes of the species Ae. Aegypti. Despite the low rate of infection in the genus Anopheles, here we detected infection in Anophelines of the subgenus Nyssorhynchus. Like Anopheles, Cellia and Lophopodomyia, Nyssorhynchus is physiologically permissive to support Wolbachia infection. |
publishDate |
2021 |
dc.date.accessioned.fl_str_mv |
2021-10-21T14:03:51Z |
dc.date.issued.fl_str_mv |
2021-07-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
CHAVES, Erick Barros. Detecção de Wolbachia em mosquitos (Diptera: Culicidae) no estado do Maranhão, Brasil. 2021. 57 f. Dissertação (Programa de Pós-Graduação em Ciências da Saúde/CCBS) - Universidade Federal do Maranhão, São Luís, 2021. |
dc.identifier.uri.fl_str_mv |
https://tedebc.ufma.br/jspui/handle/tede/tede/3379 |
identifier_str_mv |
CHAVES, Erick Barros. Detecção de Wolbachia em mosquitos (Diptera: Culicidae) no estado do Maranhão, Brasil. 2021. 57 f. Dissertação (Programa de Pós-Graduação em Ciências da Saúde/CCBS) - Universidade Federal do Maranhão, São Luís, 2021. |
url |
https://tedebc.ufma.br/jspui/handle/tede/tede/3379 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal do Maranhão |
dc.publisher.program.fl_str_mv |
PROGRAMA DE PÓS-GRADUAÇÃO EM CIÊNCIAS DA SAÚDE/CCBS |
dc.publisher.initials.fl_str_mv |
UFMA |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
DEPARTAMENTO DE BIOLOGIA/CCBS |
publisher.none.fl_str_mv |
Universidade Federal do Maranhão |
dc.source.none.fl_str_mv |
reponame:Biblioteca Digital de Teses e Dissertações da UFMA instname:Universidade Federal do Maranhão (UFMA) instacron:UFMA |
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Universidade Federal do Maranhão (UFMA) |
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UFMA |
institution |
UFMA |
reponame_str |
Biblioteca Digital de Teses e Dissertações da UFMA |
collection |
Biblioteca Digital de Teses e Dissertações da UFMA |
bitstream.url.fl_str_mv |
http://tedebc.ufma.br:8080/bitstream/tede/3379/2/ERICK-CHAVES.pdf http://tedebc.ufma.br:8080/bitstream/tede/3379/1/license.txt |
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Biblioteca Digital de Teses e Dissertações da UFMA - Universidade Federal do Maranhão (UFMA) |
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