Atividade antimicrobiana in vitro e potencial probiótico de Bifidobacterium e Lactobacillus contra espécies de Clostridium

Detalhes bibliográficos
Autor(a) principal: MONTEIRO, Cinara Regina Aragão Vieira
Data de Publicação: 2018
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações da UFMA
Texto Completo: https://tedebc.ufma.br/jspui/handle/tede/tede/2516
Resumo: Clostridium species, mainly C. butyricum, C. difficile, and C. perfringens, are agents of infectious disease resulting from intestinal dysbiosis. In many cases, recurrence can occur after antibiotics, indicating the need for novel therapeutic options that act on the pathogens and also restore the microbiota. Herein, the in vitro antimicrobial activity and probiotic potential of clinical and reference strains of Bifidobacterium and Lactobacillus were investigated against pathogenic species of Clostridium. The antimicrobial activity was evaluated by the agar spot test and inhibition of gas production. Then, the probiotic potential of selected strains was assessed by analyzing their coaggregation ability, adhesive properties to host cells and mucin, tolerance to acidic pH and bile salts, and antimicrobial susceptibility profiles. Lactobacillus plantarum ATCC 8014 was the most promising strain based on its inhibitory activity against Clostridium spp. In addition, this strain met criteria to be considered a probiotic based on its coaggregation ability, adhesive properties, and tolerance to harsh conditions of pH and bile acid salts. Overall, the results indicate that among the studied strains, L. plantarum ATCC 8014 presents probiotic potential for controlling infections by the Clostridium species studied and should be further evaluated in in vivo animal studies.
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spelling MONTEIRO NETO, Valério250376503-30http://lattes.cnpq.br/5476942147520772BOMFIM, Maria Rosa Quaresma682538188-15http://lattes.cnpq.br/6848802803471711MONTEIRO NETO, Valério250376503-30http://lattes.cnpq.br/5476942147520772ABREU JUNIOR, Afonso Gomeshttp://lattes.cnpq.br/4394413983541820MONTEIRO, Cristina de Andradehttp://lattes.cnpq.br/2495926580105868SILVA, Lucilene Amorimhttp://lattes.cnpq.br/9794797427532881651069463-87http://lattes.cnpq.br/2464038746547112MONTEIRO, Cinara Regina Aragão Vieira2019-02-11T17:19:04Z2018-12-27MONTEIRO, Cinara Regina Aragão Vieira. Atividade antimicrobiana in vitro e potencial probiótico de Bifidobacterium e Lactobacillus contra espécies de Clostridium. 2018. 70 f. Dissertação (Programa de Pós-Graduação em Ciências da Saúde/CCBS) - Universidade Federal do Maranhão, São Luís.https://tedebc.ufma.br/jspui/handle/tede/tede/2516Clostridium species, mainly C. butyricum, C. difficile, and C. perfringens, are agents of infectious disease resulting from intestinal dysbiosis. In many cases, recurrence can occur after antibiotics, indicating the need for novel therapeutic options that act on the pathogens and also restore the microbiota. Herein, the in vitro antimicrobial activity and probiotic potential of clinical and reference strains of Bifidobacterium and Lactobacillus were investigated against pathogenic species of Clostridium. The antimicrobial activity was evaluated by the agar spot test and inhibition of gas production. Then, the probiotic potential of selected strains was assessed by analyzing their coaggregation ability, adhesive properties to host cells and mucin, tolerance to acidic pH and bile salts, and antimicrobial susceptibility profiles. Lactobacillus plantarum ATCC 8014 was the most promising strain based on its inhibitory activity against Clostridium spp. In addition, this strain met criteria to be considered a probiotic based on its coaggregation ability, adhesive properties, and tolerance to harsh conditions of pH and bile acid salts. Overall, the results indicate that among the studied strains, L. plantarum ATCC 8014 presents probiotic potential for controlling infections by the Clostridium species studied and should be further evaluated in in vivo animal studies.Espécies de Clostridium, principalmente C. butyricum, C. difficile e C. perfringens, são agentes de doenças infecciosas resultantes de disbiose intestinal. Em muitos casos, a recorrência pode ocorrer após antibioticoterapia, indicando a necessidade de novas opções terapêuticas que atuem sobre os patógenos e também restaurem a microbiota. Neste trabalho, investigou-se a atividade antimicrobiana in vitro e o potencial probiótico de cepas clínicas e de referência de Bifidobacterium e Lactobacillus contra espécies patogênicas de Clostridium. A atividade antimicrobiana foi avaliada pelo ensaio de difusão em sobrecamadas e pela inibição da produção de gás. Em seguida, o potencial probiótico das cepas selecionadas foi avaliado pela análise da capacidade de coagregação, propriedades de adesão às células eucarióticas e mucina, tolerância ao pH ácido e sais biliares, e pela determinação do perfil de susceptibilidade aos antibióticos. Lactobacillus plantarum ATCC 8014 foi a cepa mais promissora baseada em sua atividade inibitória contra Clostridium spp. Além disso, esta cepa preencheu os critérios para ser considerada um probiótico com base na sua capacidade de coagregação, propriedades de adesão e tolerância a condições adversas de pH e sais de ácidos biliares. No geral, os resultados indicam que, entre as linhagens estudadas, L. plantarum ATCC 8014 apresenta potencial probiótico para o controle de infecções pelas espécies de Clostridium estudadas e deve ser melhor avaliado em estudos animaisSubmitted by Daniella Santos (daniella.santos@ufma.br) on 2019-02-11T17:19:04Z No. of bitstreams: 1 CINARAMONTEIRO.pdf: 14778385 bytes, checksum: 27ac2343241b664f31f5225666f5b3ae (MD5)Made available in DSpace on 2019-02-11T17:19:04Z (GMT). No. of bitstreams: 1 CINARAMONTEIRO.pdf: 14778385 bytes, checksum: 27ac2343241b664f31f5225666f5b3ae (MD5) Previous issue date: 2018-12-27application/pdfporUniversidade Federal do MaranhãoPROGRAMA DE PÓS-GRADUAÇÃO EM CIÊNCIAS DA SAÚDE/CCBSUFMABrasilDEPARTAMENTO DE FARMÁCIA/CCBSProbióticosLactobacillus plantarumClostridiumDisbioseAtividade antimicrobianaProbioticsLactobacillus plantarumClostridiumDysbiosisAntimicrobial activityMicrobiologiaAtividade antimicrobiana in vitro e potencial probiótico de Bifidobacterium e Lactobacillus contra espécies de ClostridiumIn vitro antimicrobial activity and probiotic potential of Bifidobacterium and Lactobacillus against Clostridium speciesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UFMAinstname:Universidade Federal do Maranhão (UFMA)instacron:UFMAORIGINALCINARAMONTEIRO.pdfCINARAMONTEIRO.pdfapplication/pdf14778385http://tedebc.ufma.br:8080/bitstream/tede/2516/2/CINARAMONTEIRO.pdf27ac2343241b664f31f5225666f5b3aeMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82255http://tedebc.ufma.br:8080/bitstream/tede/2516/1/license.txt97eeade1fce43278e63fe063657f8083MD51tede/25162019-02-11 14:19:04.549oai:tede2: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Biblioteca Digital de Teses e Dissertaçõeshttps://tedebc.ufma.br/jspui/PUBhttp://tedebc.ufma.br:8080/oai/requestrepositorio@ufma.br||repositorio@ufma.bropendoar:21312019-02-11T17:19:04Biblioteca Digital de Teses e Dissertações da UFMA - Universidade Federal do Maranhão (UFMA)false
dc.title.por.fl_str_mv Atividade antimicrobiana in vitro e potencial probiótico de Bifidobacterium e Lactobacillus contra espécies de Clostridium
dc.title.alternative.eng.fl_str_mv In vitro antimicrobial activity and probiotic potential of Bifidobacterium and Lactobacillus against Clostridium species
title Atividade antimicrobiana in vitro e potencial probiótico de Bifidobacterium e Lactobacillus contra espécies de Clostridium
spellingShingle Atividade antimicrobiana in vitro e potencial probiótico de Bifidobacterium e Lactobacillus contra espécies de Clostridium
MONTEIRO, Cinara Regina Aragão Vieira
Probióticos
Lactobacillus plantarum
Clostridium
Disbiose
Atividade antimicrobiana
Probiotics
Lactobacillus plantarum
Clostridium
Dysbiosis
Antimicrobial activity
Microbiologia
title_short Atividade antimicrobiana in vitro e potencial probiótico de Bifidobacterium e Lactobacillus contra espécies de Clostridium
title_full Atividade antimicrobiana in vitro e potencial probiótico de Bifidobacterium e Lactobacillus contra espécies de Clostridium
title_fullStr Atividade antimicrobiana in vitro e potencial probiótico de Bifidobacterium e Lactobacillus contra espécies de Clostridium
title_full_unstemmed Atividade antimicrobiana in vitro e potencial probiótico de Bifidobacterium e Lactobacillus contra espécies de Clostridium
title_sort Atividade antimicrobiana in vitro e potencial probiótico de Bifidobacterium e Lactobacillus contra espécies de Clostridium
author MONTEIRO, Cinara Regina Aragão Vieira
author_facet MONTEIRO, Cinara Regina Aragão Vieira
author_role author
dc.contributor.advisor1.fl_str_mv MONTEIRO NETO, Valério
dc.contributor.advisor1ID.fl_str_mv 250376503-30
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/5476942147520772
dc.contributor.advisor-co1.fl_str_mv BOMFIM, Maria Rosa Quaresma
dc.contributor.advisor-co1ID.fl_str_mv 682538188-15
dc.contributor.advisor-co1Lattes.fl_str_mv http://lattes.cnpq.br/6848802803471711
dc.contributor.referee1.fl_str_mv MONTEIRO NETO, Valério
dc.contributor.referee1ID.fl_str_mv 250376503-30
dc.contributor.referee1Lattes.fl_str_mv http://lattes.cnpq.br/5476942147520772
dc.contributor.referee2.fl_str_mv ABREU JUNIOR, Afonso Gomes
dc.contributor.referee2Lattes.fl_str_mv http://lattes.cnpq.br/4394413983541820
dc.contributor.referee3.fl_str_mv MONTEIRO, Cristina de Andrade
dc.contributor.referee3Lattes.fl_str_mv http://lattes.cnpq.br/2495926580105868
dc.contributor.referee4.fl_str_mv SILVA, Lucilene Amorim
dc.contributor.referee4Lattes.fl_str_mv http://lattes.cnpq.br/9794797427532881
dc.contributor.authorID.fl_str_mv 651069463-87
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/2464038746547112
dc.contributor.author.fl_str_mv MONTEIRO, Cinara Regina Aragão Vieira
contributor_str_mv MONTEIRO NETO, Valério
BOMFIM, Maria Rosa Quaresma
MONTEIRO NETO, Valério
ABREU JUNIOR, Afonso Gomes
MONTEIRO, Cristina de Andrade
SILVA, Lucilene Amorim
dc.subject.por.fl_str_mv Probióticos
Lactobacillus plantarum
Clostridium
Disbiose
Atividade antimicrobiana
topic Probióticos
Lactobacillus plantarum
Clostridium
Disbiose
Atividade antimicrobiana
Probiotics
Lactobacillus plantarum
Clostridium
Dysbiosis
Antimicrobial activity
Microbiologia
dc.subject.eng.fl_str_mv Probiotics
Lactobacillus plantarum
Clostridium
Dysbiosis
Antimicrobial activity
dc.subject.cnpq.fl_str_mv Microbiologia
description Clostridium species, mainly C. butyricum, C. difficile, and C. perfringens, are agents of infectious disease resulting from intestinal dysbiosis. In many cases, recurrence can occur after antibiotics, indicating the need for novel therapeutic options that act on the pathogens and also restore the microbiota. Herein, the in vitro antimicrobial activity and probiotic potential of clinical and reference strains of Bifidobacterium and Lactobacillus were investigated against pathogenic species of Clostridium. The antimicrobial activity was evaluated by the agar spot test and inhibition of gas production. Then, the probiotic potential of selected strains was assessed by analyzing their coaggregation ability, adhesive properties to host cells and mucin, tolerance to acidic pH and bile salts, and antimicrobial susceptibility profiles. Lactobacillus plantarum ATCC 8014 was the most promising strain based on its inhibitory activity against Clostridium spp. In addition, this strain met criteria to be considered a probiotic based on its coaggregation ability, adhesive properties, and tolerance to harsh conditions of pH and bile acid salts. Overall, the results indicate that among the studied strains, L. plantarum ATCC 8014 presents probiotic potential for controlling infections by the Clostridium species studied and should be further evaluated in in vivo animal studies.
publishDate 2018
dc.date.issued.fl_str_mv 2018-12-27
dc.date.accessioned.fl_str_mv 2019-02-11T17:19:04Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
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dc.identifier.citation.fl_str_mv MONTEIRO, Cinara Regina Aragão Vieira. Atividade antimicrobiana in vitro e potencial probiótico de Bifidobacterium e Lactobacillus contra espécies de Clostridium. 2018. 70 f. Dissertação (Programa de Pós-Graduação em Ciências da Saúde/CCBS) - Universidade Federal do Maranhão, São Luís.
dc.identifier.uri.fl_str_mv https://tedebc.ufma.br/jspui/handle/tede/tede/2516
identifier_str_mv MONTEIRO, Cinara Regina Aragão Vieira. Atividade antimicrobiana in vitro e potencial probiótico de Bifidobacterium e Lactobacillus contra espécies de Clostridium. 2018. 70 f. Dissertação (Programa de Pós-Graduação em Ciências da Saúde/CCBS) - Universidade Federal do Maranhão, São Luís.
url https://tedebc.ufma.br/jspui/handle/tede/tede/2516
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dc.publisher.none.fl_str_mv Universidade Federal do Maranhão
dc.publisher.program.fl_str_mv PROGRAMA DE PÓS-GRADUAÇÃO EM CIÊNCIAS DA SAÚDE/CCBS
dc.publisher.initials.fl_str_mv UFMA
dc.publisher.country.fl_str_mv Brasil
dc.publisher.department.fl_str_mv DEPARTAMENTO DE FARMÁCIA/CCBS
publisher.none.fl_str_mv Universidade Federal do Maranhão
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