Molecular analysis of the N gene of canine distemper virus in dogs in Brazil

Detalhes bibliográficos
Autor(a) principal: Castilho,J.G.
Data de Publicação: 2007
Outros Autores: Brandão,P.E., Carnieli Jr,P., Oliveira,R.N., Macedo,C.I., Peixoto,Z.M.P., Carrieri,M.L., Kotait,I.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Arquivo brasileiro de medicina veterinária e zootecnia (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352007000300016
Resumo: Eleven central-nervous-system samples collected from stray dogs between 2000 and 2004 were found positive by RT-PCR, which amplified a 480bp fragment of the N gene of canine distemper virus (CDV). Phylogenetic analysis based on partial N-gene sequences showed four major clusters. All dog strains segregated into cluster I, with a mean nucleotide identity of 95.8% and 95.6% with the Onderstepoort and Lederle vaccine strains, respectively. Cluster II contained all the raccoon-related strains, cluster III Orient strains and Cluster IV the Onderstepoort and Lederle vaccine strains, with a mean nucleotide identity of 99.7% between them. This is the first report of phylogenetic analysis of CDV strains in Brazil.
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spelling Molecular analysis of the N gene of canine distemper virus in dogs in Brazildogcanine distemper virusRT-PCRphylogenyEleven central-nervous-system samples collected from stray dogs between 2000 and 2004 were found positive by RT-PCR, which amplified a 480bp fragment of the N gene of canine distemper virus (CDV). Phylogenetic analysis based on partial N-gene sequences showed four major clusters. All dog strains segregated into cluster I, with a mean nucleotide identity of 95.8% and 95.6% with the Onderstepoort and Lederle vaccine strains, respectively. Cluster II contained all the raccoon-related strains, cluster III Orient strains and Cluster IV the Onderstepoort and Lederle vaccine strains, with a mean nucleotide identity of 99.7% between them. This is the first report of phylogenetic analysis of CDV strains in Brazil.Universidade Federal de Minas Gerais, Escola de Veterinária2007-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352007000300016Arquivo Brasileiro de Medicina Veterinária e Zootecnia v.59 n.3 2007reponame:Arquivo brasileiro de medicina veterinária e zootecnia (Online)instname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMG10.1590/S0102-09352007000300016info:eu-repo/semantics/openAccessCastilho,J.G.Brandão,P.E.Carnieli Jr,P.Oliveira,R.N.Macedo,C.I.Peixoto,Z.M.P.Carrieri,M.L.Kotait,I.eng2007-09-19T00:00:00Zoai:scielo:S0102-09352007000300016Revistahttps://www.scielo.br/j/abmvz/PUBhttps://old.scielo.br/oai/scielo-oai.phpjournal@vet.ufmg.br||abmvz.artigo@abmvz.org.br1678-41620102-0935opendoar:2007-09-19T00:00Arquivo brasileiro de medicina veterinária e zootecnia (Online) - Universidade Federal de Minas Gerais (UFMG)false
dc.title.none.fl_str_mv Molecular analysis of the N gene of canine distemper virus in dogs in Brazil
title Molecular analysis of the N gene of canine distemper virus in dogs in Brazil
spellingShingle Molecular analysis of the N gene of canine distemper virus in dogs in Brazil
Castilho,J.G.
dog
canine distemper virus
RT-PCR
phylogeny
title_short Molecular analysis of the N gene of canine distemper virus in dogs in Brazil
title_full Molecular analysis of the N gene of canine distemper virus in dogs in Brazil
title_fullStr Molecular analysis of the N gene of canine distemper virus in dogs in Brazil
title_full_unstemmed Molecular analysis of the N gene of canine distemper virus in dogs in Brazil
title_sort Molecular analysis of the N gene of canine distemper virus in dogs in Brazil
author Castilho,J.G.
author_facet Castilho,J.G.
Brandão,P.E.
Carnieli Jr,P.
Oliveira,R.N.
Macedo,C.I.
Peixoto,Z.M.P.
Carrieri,M.L.
Kotait,I.
author_role author
author2 Brandão,P.E.
Carnieli Jr,P.
Oliveira,R.N.
Macedo,C.I.
Peixoto,Z.M.P.
Carrieri,M.L.
Kotait,I.
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Castilho,J.G.
Brandão,P.E.
Carnieli Jr,P.
Oliveira,R.N.
Macedo,C.I.
Peixoto,Z.M.P.
Carrieri,M.L.
Kotait,I.
dc.subject.por.fl_str_mv dog
canine distemper virus
RT-PCR
phylogeny
topic dog
canine distemper virus
RT-PCR
phylogeny
description Eleven central-nervous-system samples collected from stray dogs between 2000 and 2004 were found positive by RT-PCR, which amplified a 480bp fragment of the N gene of canine distemper virus (CDV). Phylogenetic analysis based on partial N-gene sequences showed four major clusters. All dog strains segregated into cluster I, with a mean nucleotide identity of 95.8% and 95.6% with the Onderstepoort and Lederle vaccine strains, respectively. Cluster II contained all the raccoon-related strains, cluster III Orient strains and Cluster IV the Onderstepoort and Lederle vaccine strains, with a mean nucleotide identity of 99.7% between them. This is the first report of phylogenetic analysis of CDV strains in Brazil.
publishDate 2007
dc.date.none.fl_str_mv 2007-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352007000300016
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352007000300016
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0102-09352007000300016
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Universidade Federal de Minas Gerais, Escola de Veterinária
publisher.none.fl_str_mv Universidade Federal de Minas Gerais, Escola de Veterinária
dc.source.none.fl_str_mv Arquivo Brasileiro de Medicina Veterinária e Zootecnia v.59 n.3 2007
reponame:Arquivo brasileiro de medicina veterinária e zootecnia (Online)
instname:Universidade Federal de Minas Gerais (UFMG)
instacron:UFMG
instname_str Universidade Federal de Minas Gerais (UFMG)
instacron_str UFMG
institution UFMG
reponame_str Arquivo brasileiro de medicina veterinária e zootecnia (Online)
collection Arquivo brasileiro de medicina veterinária e zootecnia (Online)
repository.name.fl_str_mv Arquivo brasileiro de medicina veterinária e zootecnia (Online) - Universidade Federal de Minas Gerais (UFMG)
repository.mail.fl_str_mv journal@vet.ufmg.br||abmvz.artigo@abmvz.org.br
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