Detection of mouse hepatitis virus in mouse colonies using the nested polymerase chain reaction

Detalhes bibliográficos
Autor(a) principal: Cecílio,A.B.
Data de Publicação: 2000
Outros Autores: Cândido,A.L., Resende,M., Bontempo,E.D., Martins,A.S.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Arquivo brasileiro de medicina veterinária e zootecnia (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352000000400003
Resumo: A reverse transcriptase polymerase chain reaction (RT-PCR) to detect mouse hepatitis virus (MHV) in hepatic tissue was developed. To circumvent possible failures in RT-PCR amplifications, a second round of PCR with internal primers was used to confirm the specificity and increase the sensitivity of the test. Using this method specific amplification of MHV sequences was observed in 18 out of 20 mouse colonies examined.
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spelling Detection of mouse hepatitis virus in mouse colonies using the nested polymerase chain reactionMouse hepatitis virusRT-PCRA reverse transcriptase polymerase chain reaction (RT-PCR) to detect mouse hepatitis virus (MHV) in hepatic tissue was developed. To circumvent possible failures in RT-PCR amplifications, a second round of PCR with internal primers was used to confirm the specificity and increase the sensitivity of the test. Using this method specific amplification of MHV sequences was observed in 18 out of 20 mouse colonies examined.Universidade Federal de Minas Gerais, Escola de Veterinária2000-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352000000400003Arquivo Brasileiro de Medicina Veterinária e Zootecnia v.52 n.4 2000reponame:Arquivo brasileiro de medicina veterinária e zootecnia (Online)instname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMG10.1590/S0102-09352000000400003info:eu-repo/semantics/openAccessCecílio,A.B.Cândido,A.L.Resende,M.Bontempo,E.D.Martins,A.S.eng2000-09-21T00:00:00Zoai:scielo:S0102-09352000000400003Revistahttps://www.scielo.br/j/abmvz/PUBhttps://old.scielo.br/oai/scielo-oai.phpjournal@vet.ufmg.br||abmvz.artigo@abmvz.org.br1678-41620102-0935opendoar:2000-09-21T00:00Arquivo brasileiro de medicina veterinária e zootecnia (Online) - Universidade Federal de Minas Gerais (UFMG)false
dc.title.none.fl_str_mv Detection of mouse hepatitis virus in mouse colonies using the nested polymerase chain reaction
title Detection of mouse hepatitis virus in mouse colonies using the nested polymerase chain reaction
spellingShingle Detection of mouse hepatitis virus in mouse colonies using the nested polymerase chain reaction
Cecílio,A.B.
Mouse hepatitis virus
RT-PCR
title_short Detection of mouse hepatitis virus in mouse colonies using the nested polymerase chain reaction
title_full Detection of mouse hepatitis virus in mouse colonies using the nested polymerase chain reaction
title_fullStr Detection of mouse hepatitis virus in mouse colonies using the nested polymerase chain reaction
title_full_unstemmed Detection of mouse hepatitis virus in mouse colonies using the nested polymerase chain reaction
title_sort Detection of mouse hepatitis virus in mouse colonies using the nested polymerase chain reaction
author Cecílio,A.B.
author_facet Cecílio,A.B.
Cândido,A.L.
Resende,M.
Bontempo,E.D.
Martins,A.S.
author_role author
author2 Cândido,A.L.
Resende,M.
Bontempo,E.D.
Martins,A.S.
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Cecílio,A.B.
Cândido,A.L.
Resende,M.
Bontempo,E.D.
Martins,A.S.
dc.subject.por.fl_str_mv Mouse hepatitis virus
RT-PCR
topic Mouse hepatitis virus
RT-PCR
description A reverse transcriptase polymerase chain reaction (RT-PCR) to detect mouse hepatitis virus (MHV) in hepatic tissue was developed. To circumvent possible failures in RT-PCR amplifications, a second round of PCR with internal primers was used to confirm the specificity and increase the sensitivity of the test. Using this method specific amplification of MHV sequences was observed in 18 out of 20 mouse colonies examined.
publishDate 2000
dc.date.none.fl_str_mv 2000-08-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352000000400003
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352000000400003
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0102-09352000000400003
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Universidade Federal de Minas Gerais, Escola de Veterinária
publisher.none.fl_str_mv Universidade Federal de Minas Gerais, Escola de Veterinária
dc.source.none.fl_str_mv Arquivo Brasileiro de Medicina Veterinária e Zootecnia v.52 n.4 2000
reponame:Arquivo brasileiro de medicina veterinária e zootecnia (Online)
instname:Universidade Federal de Minas Gerais (UFMG)
instacron:UFMG
instname_str Universidade Federal de Minas Gerais (UFMG)
instacron_str UFMG
institution UFMG
reponame_str Arquivo brasileiro de medicina veterinária e zootecnia (Online)
collection Arquivo brasileiro de medicina veterinária e zootecnia (Online)
repository.name.fl_str_mv Arquivo brasileiro de medicina veterinária e zootecnia (Online) - Universidade Federal de Minas Gerais (UFMG)
repository.mail.fl_str_mv journal@vet.ufmg.br||abmvz.artigo@abmvz.org.br
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