Feline immunodeficiency virusinfection: a comparative study of different diagnostic techniques

Detalhes bibliográficos
Autor(a) principal: Mortola,E.
Data de Publicação: 2004
Outros Autores: Oliva,G., Risso,M., Pecoraro,M., Venturini,M.C.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Arquivo brasileiro de medicina veterinária e zootecnia (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352004000100003
Resumo: This study evaluated an indirect immunofluorescence assay (IFA) to detect feline immunodeficiency virus infection (FIV) antibody in a comprehensive epidemiological survey of FIV in Argentina. IFA modified in our laboratory, was compared with two other immunoassays, western blot (WB) and a sandwich immunochromatographic commercial kit (SI), and also with a direct polymerase chain reaction (PCR) method that detects proviral DNA. IFA showed to be a test with high sensitivity and specificity, and could be useful as a diagnostic tool in epidemiological studies. The presence of a low percentage of results with non-specific reactivity in the IFA could be resolved with further testing or use of an alternative method.
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spelling Feline immunodeficiency virusinfection: a comparative study of different diagnostic techniquesfeline immunodeficiency virusimmunofluorescence assaywestern blotpolymerase chain reactionThis study evaluated an indirect immunofluorescence assay (IFA) to detect feline immunodeficiency virus infection (FIV) antibody in a comprehensive epidemiological survey of FIV in Argentina. IFA modified in our laboratory, was compared with two other immunoassays, western blot (WB) and a sandwich immunochromatographic commercial kit (SI), and also with a direct polymerase chain reaction (PCR) method that detects proviral DNA. IFA showed to be a test with high sensitivity and specificity, and could be useful as a diagnostic tool in epidemiological studies. The presence of a low percentage of results with non-specific reactivity in the IFA could be resolved with further testing or use of an alternative method.Universidade Federal de Minas Gerais, Escola de Veterinária2004-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352004000100003Arquivo Brasileiro de Medicina Veterinária e Zootecnia v.56 n.1 2004reponame:Arquivo brasileiro de medicina veterinária e zootecnia (Online)instname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMG10.1590/S0102-09352004000100003info:eu-repo/semantics/openAccessMortola,E.Oliva,G.Risso,M.Pecoraro,M.Venturini,M.C.eng2004-06-16T00:00:00Zoai:scielo:S0102-09352004000100003Revistahttps://www.scielo.br/j/abmvz/PUBhttps://old.scielo.br/oai/scielo-oai.phpjournal@vet.ufmg.br||abmvz.artigo@abmvz.org.br1678-41620102-0935opendoar:2004-06-16T00:00Arquivo brasileiro de medicina veterinária e zootecnia (Online) - Universidade Federal de Minas Gerais (UFMG)false
dc.title.none.fl_str_mv Feline immunodeficiency virusinfection: a comparative study of different diagnostic techniques
title Feline immunodeficiency virusinfection: a comparative study of different diagnostic techniques
spellingShingle Feline immunodeficiency virusinfection: a comparative study of different diagnostic techniques
Mortola,E.
feline immunodeficiency virus
immunofluorescence assay
western blot
polymerase chain reaction
title_short Feline immunodeficiency virusinfection: a comparative study of different diagnostic techniques
title_full Feline immunodeficiency virusinfection: a comparative study of different diagnostic techniques
title_fullStr Feline immunodeficiency virusinfection: a comparative study of different diagnostic techniques
title_full_unstemmed Feline immunodeficiency virusinfection: a comparative study of different diagnostic techniques
title_sort Feline immunodeficiency virusinfection: a comparative study of different diagnostic techniques
author Mortola,E.
author_facet Mortola,E.
Oliva,G.
Risso,M.
Pecoraro,M.
Venturini,M.C.
author_role author
author2 Oliva,G.
Risso,M.
Pecoraro,M.
Venturini,M.C.
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Mortola,E.
Oliva,G.
Risso,M.
Pecoraro,M.
Venturini,M.C.
dc.subject.por.fl_str_mv feline immunodeficiency virus
immunofluorescence assay
western blot
polymerase chain reaction
topic feline immunodeficiency virus
immunofluorescence assay
western blot
polymerase chain reaction
description This study evaluated an indirect immunofluorescence assay (IFA) to detect feline immunodeficiency virus infection (FIV) antibody in a comprehensive epidemiological survey of FIV in Argentina. IFA modified in our laboratory, was compared with two other immunoassays, western blot (WB) and a sandwich immunochromatographic commercial kit (SI), and also with a direct polymerase chain reaction (PCR) method that detects proviral DNA. IFA showed to be a test with high sensitivity and specificity, and could be useful as a diagnostic tool in epidemiological studies. The presence of a low percentage of results with non-specific reactivity in the IFA could be resolved with further testing or use of an alternative method.
publishDate 2004
dc.date.none.fl_str_mv 2004-02-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352004000100003
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352004000100003
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0102-09352004000100003
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Universidade Federal de Minas Gerais, Escola de Veterinária
publisher.none.fl_str_mv Universidade Federal de Minas Gerais, Escola de Veterinária
dc.source.none.fl_str_mv Arquivo Brasileiro de Medicina Veterinária e Zootecnia v.56 n.1 2004
reponame:Arquivo brasileiro de medicina veterinária e zootecnia (Online)
instname:Universidade Federal de Minas Gerais (UFMG)
instacron:UFMG
instname_str Universidade Federal de Minas Gerais (UFMG)
instacron_str UFMG
institution UFMG
reponame_str Arquivo brasileiro de medicina veterinária e zootecnia (Online)
collection Arquivo brasileiro de medicina veterinária e zootecnia (Online)
repository.name.fl_str_mv Arquivo brasileiro de medicina veterinária e zootecnia (Online) - Universidade Federal de Minas Gerais (UFMG)
repository.mail.fl_str_mv journal@vet.ufmg.br||abmvz.artigo@abmvz.org.br
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