Development of an indirect ELISA for the diagnosis of Feline Immunodeficiency Virus
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Brazilian Journal of Veterinary Medicine |
Texto Completo: | https://rbmv.org/BJVM/article/view/609 |
Resumo: | ABSTRACT. Alves F., Mazur C., Rajão D. de S., Diniz R.S., Del Puerto H.L., Braz G.F., Vieira F. de O., Leite R.C., Martins A. de S. & dos Reis J.K.P. Development of an indirect ELISA for the diagnosis of Feline Immunodeficiency Virus. [Desenvolvimento de um ELISA indireto para o diagnóstico do Vírus da Imunodeficiência Felina]. Revista Brasileira de Medicina Veterinária, 35(3):241-246, 2013. Departamento de Fisiologia e Biofísica, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, Pampulha, Belo Horizonte, MG 31270-901, Brasil. E-mail: alves.bio@gmail.com The objective of this study was to develop an indirect ELISA (r-p24 ELISA) for the diagnosis of Feline Immunodeficiency virus (FIV-B). Serum samples of 150 asymptomatic cats, of both sexes and mixed breeds and ages were used. The SNAP Combo Plus commercial kit was used as the gold standard. The r-p24 ELISA was standardized using 0.25 µg of recombinant p24 protein (r-p24) per well, serum was diluted at 1:320 and the conjugate was diluted at 1:7500. The r-p24 ELISA showed a sensitivity of 97% and specificity of 93% when compared to the gold standard with a concordance of 83%. When compared to the Western blot confirmatory test, the r-p24 ELISA showed a sensitivity of 94% and specificity of 94% with a concordance of 84%. Therefore, the standardized ELISA is an important tool for the FIV related research and for the FIV diagnosis at low cost. |
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Development of an indirect ELISA for the diagnosis of Feline Immunodeficiency VirusDEVELOPMENT OF AN INDIRECT ELISA FOR THE DIAGNOSIS OF FELINE IMMUNODEFICIENCY VIRUSVírus da imunodeficiência felinaproteína p-24Western blotELISAFeline immunodeficiency virusp-24 proteinWestern blotELISAABSTRACT. Alves F., Mazur C., Rajão D. de S., Diniz R.S., Del Puerto H.L., Braz G.F., Vieira F. de O., Leite R.C., Martins A. de S. & dos Reis J.K.P. Development of an indirect ELISA for the diagnosis of Feline Immunodeficiency Virus. [Desenvolvimento de um ELISA indireto para o diagnóstico do Vírus da Imunodeficiência Felina]. Revista Brasileira de Medicina Veterinária, 35(3):241-246, 2013. Departamento de Fisiologia e Biofísica, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, Pampulha, Belo Horizonte, MG 31270-901, Brasil. E-mail: alves.bio@gmail.com The objective of this study was to develop an indirect ELISA (r-p24 ELISA) for the diagnosis of Feline Immunodeficiency virus (FIV-B). Serum samples of 150 asymptomatic cats, of both sexes and mixed breeds and ages were used. The SNAP Combo Plus commercial kit was used as the gold standard. The r-p24 ELISA was standardized using 0.25 µg of recombinant p24 protein (r-p24) per well, serum was diluted at 1:320 and the conjugate was diluted at 1:7500. The r-p24 ELISA showed a sensitivity of 97% and specificity of 93% when compared to the gold standard with a concordance of 83%. When compared to the Western blot confirmatory test, the r-p24 ELISA showed a sensitivity of 94% and specificity of 94% with a concordance of 84%. Therefore, the standardized ELISA is an important tool for the FIV related research and for the FIV diagnosis at low cost.O objetivo deste estudo foi desenvolver um ELISA indireto (r-p24 ELISA) para o diagnóstico do Vírus da Imunodeficiência Felina (FIV). Foram utilizadas 150 amostras de soro de animais assintomáticos, sem distinção de sexo e idade. O kit comercial SNAP Combo Plus foi utilizado como padrão ouro. O r-p24 ELISA foi padronizado utilizando 0,25 µg da proteína recombinante p24 (r-p24) por poço, soro na diluição de 1:320 e o conjugado na diluição de 1:7500. O r-p24 ELISA mostrou um sensibilidade de 97% e uma especificidade de 93% e uma concordância de 83%, quando comparado ao padrão ouro. Quando comparado ao Western blot que é o teste confirmatório, o r-p24 ELISA mostrou sensibilidade de 94% e especificidade de 94% com concordância de 84%. Contudo, o ELISA padronizado é uma importante ferramenta para as pesquisas relacionadas com o FIV e para o diagnóstico a baixo custo.Sociedade de Medicina Veterinária do Estado do Rio de Janeiro.2013-09-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionpeer reviewedAvaliado pelos paresapplication/pdfhttps://rbmv.org/BJVM/article/view/609Brazilian Journal of Veterinary Medicine; Vol. 35 No. 3 (2013); 241-246Revista Brasileira de Medicina Veterinária; v. 35 n. 3 (2013); 241-2462527-21790100-2430reponame:Brazilian Journal of Veterinary Medicineinstname:Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ)instacron:SBMVporhttps://rbmv.org/BJVM/article/view/609/472Alves, FabianaMazur, CarlosRajão, Daniela de SouzaDiniz, Rejane SilvaDel Puerto, Helen LimaBraz, Gissandra FariasVieira, Francisco de OliveiraLeite, Rômulo CerqueiraMartins, Almir de Sousados Reis, Jenner Karlisson Pimentainfo:eu-repo/semantics/openAccess2020-12-23T17:30:40Zoai:ojs.rbmv.org:article/609Revistahttps://rbmv.org/BJVMONGhttps://rbmv.org/BJVM/oaicontato.rbmv@gmail.com2527-21790100-2430opendoar:2020-12-23T17:30:40Brazilian Journal of Veterinary Medicine - Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ)false |
dc.title.none.fl_str_mv |
Development of an indirect ELISA for the diagnosis of Feline Immunodeficiency Virus DEVELOPMENT OF AN INDIRECT ELISA FOR THE DIAGNOSIS OF FELINE IMMUNODEFICIENCY VIRUS |
title |
Development of an indirect ELISA for the diagnosis of Feline Immunodeficiency Virus |
spellingShingle |
Development of an indirect ELISA for the diagnosis of Feline Immunodeficiency Virus Alves, Fabiana Vírus da imunodeficiência felina proteína p-24 Western blot ELISA Feline immunodeficiency virus p-24 protein Western blot ELISA |
title_short |
Development of an indirect ELISA for the diagnosis of Feline Immunodeficiency Virus |
title_full |
Development of an indirect ELISA for the diagnosis of Feline Immunodeficiency Virus |
title_fullStr |
Development of an indirect ELISA for the diagnosis of Feline Immunodeficiency Virus |
title_full_unstemmed |
Development of an indirect ELISA for the diagnosis of Feline Immunodeficiency Virus |
title_sort |
Development of an indirect ELISA for the diagnosis of Feline Immunodeficiency Virus |
author |
Alves, Fabiana |
author_facet |
Alves, Fabiana Mazur, Carlos Rajão, Daniela de Souza Diniz, Rejane Silva Del Puerto, Helen Lima Braz, Gissandra Farias Vieira, Francisco de Oliveira Leite, Rômulo Cerqueira Martins, Almir de Sousa dos Reis, Jenner Karlisson Pimenta |
author_role |
author |
author2 |
Mazur, Carlos Rajão, Daniela de Souza Diniz, Rejane Silva Del Puerto, Helen Lima Braz, Gissandra Farias Vieira, Francisco de Oliveira Leite, Rômulo Cerqueira Martins, Almir de Sousa dos Reis, Jenner Karlisson Pimenta |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Alves, Fabiana Mazur, Carlos Rajão, Daniela de Souza Diniz, Rejane Silva Del Puerto, Helen Lima Braz, Gissandra Farias Vieira, Francisco de Oliveira Leite, Rômulo Cerqueira Martins, Almir de Sousa dos Reis, Jenner Karlisson Pimenta |
dc.subject.por.fl_str_mv |
Vírus da imunodeficiência felina proteína p-24 Western blot ELISA Feline immunodeficiency virus p-24 protein Western blot ELISA |
topic |
Vírus da imunodeficiência felina proteína p-24 Western blot ELISA Feline immunodeficiency virus p-24 protein Western blot ELISA |
description |
ABSTRACT. Alves F., Mazur C., Rajão D. de S., Diniz R.S., Del Puerto H.L., Braz G.F., Vieira F. de O., Leite R.C., Martins A. de S. & dos Reis J.K.P. Development of an indirect ELISA for the diagnosis of Feline Immunodeficiency Virus. [Desenvolvimento de um ELISA indireto para o diagnóstico do Vírus da Imunodeficiência Felina]. Revista Brasileira de Medicina Veterinária, 35(3):241-246, 2013. Departamento de Fisiologia e Biofísica, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, Pampulha, Belo Horizonte, MG 31270-901, Brasil. E-mail: alves.bio@gmail.com The objective of this study was to develop an indirect ELISA (r-p24 ELISA) for the diagnosis of Feline Immunodeficiency virus (FIV-B). Serum samples of 150 asymptomatic cats, of both sexes and mixed breeds and ages were used. The SNAP Combo Plus commercial kit was used as the gold standard. The r-p24 ELISA was standardized using 0.25 µg of recombinant p24 protein (r-p24) per well, serum was diluted at 1:320 and the conjugate was diluted at 1:7500. The r-p24 ELISA showed a sensitivity of 97% and specificity of 93% when compared to the gold standard with a concordance of 83%. When compared to the Western blot confirmatory test, the r-p24 ELISA showed a sensitivity of 94% and specificity of 94% with a concordance of 84%. Therefore, the standardized ELISA is an important tool for the FIV related research and for the FIV diagnosis at low cost. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-09-30 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion peer reviewed Avaliado pelos pares |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rbmv.org/BJVM/article/view/609 |
url |
https://rbmv.org/BJVM/article/view/609 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://rbmv.org/BJVM/article/view/609/472 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Sociedade de Medicina Veterinária do Estado do Rio de Janeiro. |
publisher.none.fl_str_mv |
Sociedade de Medicina Veterinária do Estado do Rio de Janeiro. |
dc.source.none.fl_str_mv |
Brazilian Journal of Veterinary Medicine; Vol. 35 No. 3 (2013); 241-246 Revista Brasileira de Medicina Veterinária; v. 35 n. 3 (2013); 241-246 2527-2179 0100-2430 reponame:Brazilian Journal of Veterinary Medicine instname:Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ) instacron:SBMV |
instname_str |
Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ) |
instacron_str |
SBMV |
institution |
SBMV |
reponame_str |
Brazilian Journal of Veterinary Medicine |
collection |
Brazilian Journal of Veterinary Medicine |
repository.name.fl_str_mv |
Brazilian Journal of Veterinary Medicine - Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ) |
repository.mail.fl_str_mv |
contato.rbmv@gmail.com |
_version_ |
1798313108747321344 |