Malassezia pachydermatis: enzymes production in isolates from external ear canal of dogs with and without otitis

Detalhes bibliográficos
Autor(a) principal: Coutinho,S.D.A.
Data de Publicação: 2005
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Arquivo brasileiro de medicina veterinária e zootecnia (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352005000800003
Resumo: The relationship between production of enzymes and pathogenicity of Malassezia pachydermatis strains was studied. Thirty strains of M. pachydermatis isolated from dogs were used: 15 originating from animals with external otitis and 15 obtained from ears of asymptomatic dogs. The samples, obtained by introduction of a sterile swab into the auditory canal after cleaning the auricle with alcohol-ether solution, were seeded onto Sabouraud dextrose agar with chloramphenicol (100mg/l) and were incubated at 37°C for up to two weeks. The colonies were identified according to their macro-and-micromorphology. All strains were investigated for the production of chondroitin-sulphatase, hyaluronidase, phospholipase and proteinase. Most of the strains showed a high production of the four enzymes. Statistical analysis did not show any differences in the enzymatic activity among the strains. M. pachydermatis originating in normal microbiota as well as in otic secretion from dogs with otitis could produce the four researched enzymes. Probably M. pachydermatis has other virulence factors that are involved in the parasitism.
id UFMG-8_54de4f9a98913d913509c759b4bbcf32
oai_identifier_str oai:scielo:S0102-09352005000800003
network_acronym_str UFMG-8
network_name_str Arquivo brasileiro de medicina veterinária e zootecnia (Online)
repository_id_str
spelling Malassezia pachydermatis: enzymes production in isolates from external ear canal of dogs with and without otitisdogMalassezia pachydermatisenzymesotitismicrobiotaThe relationship between production of enzymes and pathogenicity of Malassezia pachydermatis strains was studied. Thirty strains of M. pachydermatis isolated from dogs were used: 15 originating from animals with external otitis and 15 obtained from ears of asymptomatic dogs. The samples, obtained by introduction of a sterile swab into the auditory canal after cleaning the auricle with alcohol-ether solution, were seeded onto Sabouraud dextrose agar with chloramphenicol (100mg/l) and were incubated at 37°C for up to two weeks. The colonies were identified according to their macro-and-micromorphology. All strains were investigated for the production of chondroitin-sulphatase, hyaluronidase, phospholipase and proteinase. Most of the strains showed a high production of the four enzymes. Statistical analysis did not show any differences in the enzymatic activity among the strains. M. pachydermatis originating in normal microbiota as well as in otic secretion from dogs with otitis could produce the four researched enzymes. Probably M. pachydermatis has other virulence factors that are involved in the parasitism.Universidade Federal de Minas Gerais, Escola de Veterinária2005-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352005000800003Arquivo Brasileiro de Medicina Veterinária e Zootecnia v.57 suppl.2 2005reponame:Arquivo brasileiro de medicina veterinária e zootecnia (Online)instname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMG10.1590/S0102-09352005000800003info:eu-repo/semantics/openAccessCoutinho,S.D.A.eng2006-03-27T00:00:00Zoai:scielo:S0102-09352005000800003Revistahttps://www.scielo.br/j/abmvz/PUBhttps://old.scielo.br/oai/scielo-oai.phpjournal@vet.ufmg.br||abmvz.artigo@abmvz.org.br1678-41620102-0935opendoar:2006-03-27T00:00Arquivo brasileiro de medicina veterinária e zootecnia (Online) - Universidade Federal de Minas Gerais (UFMG)false
dc.title.none.fl_str_mv Malassezia pachydermatis: enzymes production in isolates from external ear canal of dogs with and without otitis
title Malassezia pachydermatis: enzymes production in isolates from external ear canal of dogs with and without otitis
spellingShingle Malassezia pachydermatis: enzymes production in isolates from external ear canal of dogs with and without otitis
Coutinho,S.D.A.
dog
Malassezia pachydermatis
enzymes
otitis
microbiota
title_short Malassezia pachydermatis: enzymes production in isolates from external ear canal of dogs with and without otitis
title_full Malassezia pachydermatis: enzymes production in isolates from external ear canal of dogs with and without otitis
title_fullStr Malassezia pachydermatis: enzymes production in isolates from external ear canal of dogs with and without otitis
title_full_unstemmed Malassezia pachydermatis: enzymes production in isolates from external ear canal of dogs with and without otitis
title_sort Malassezia pachydermatis: enzymes production in isolates from external ear canal of dogs with and without otitis
author Coutinho,S.D.A.
author_facet Coutinho,S.D.A.
author_role author
dc.contributor.author.fl_str_mv Coutinho,S.D.A.
dc.subject.por.fl_str_mv dog
Malassezia pachydermatis
enzymes
otitis
microbiota
topic dog
Malassezia pachydermatis
enzymes
otitis
microbiota
description The relationship between production of enzymes and pathogenicity of Malassezia pachydermatis strains was studied. Thirty strains of M. pachydermatis isolated from dogs were used: 15 originating from animals with external otitis and 15 obtained from ears of asymptomatic dogs. The samples, obtained by introduction of a sterile swab into the auditory canal after cleaning the auricle with alcohol-ether solution, were seeded onto Sabouraud dextrose agar with chloramphenicol (100mg/l) and were incubated at 37°C for up to two weeks. The colonies were identified according to their macro-and-micromorphology. All strains were investigated for the production of chondroitin-sulphatase, hyaluronidase, phospholipase and proteinase. Most of the strains showed a high production of the four enzymes. Statistical analysis did not show any differences in the enzymatic activity among the strains. M. pachydermatis originating in normal microbiota as well as in otic secretion from dogs with otitis could produce the four researched enzymes. Probably M. pachydermatis has other virulence factors that are involved in the parasitism.
publishDate 2005
dc.date.none.fl_str_mv 2005-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352005000800003
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352005000800003
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0102-09352005000800003
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Universidade Federal de Minas Gerais, Escola de Veterinária
publisher.none.fl_str_mv Universidade Federal de Minas Gerais, Escola de Veterinária
dc.source.none.fl_str_mv Arquivo Brasileiro de Medicina Veterinária e Zootecnia v.57 suppl.2 2005
reponame:Arquivo brasileiro de medicina veterinária e zootecnia (Online)
instname:Universidade Federal de Minas Gerais (UFMG)
instacron:UFMG
instname_str Universidade Federal de Minas Gerais (UFMG)
instacron_str UFMG
institution UFMG
reponame_str Arquivo brasileiro de medicina veterinária e zootecnia (Online)
collection Arquivo brasileiro de medicina veterinária e zootecnia (Online)
repository.name.fl_str_mv Arquivo brasileiro de medicina veterinária e zootecnia (Online) - Universidade Federal de Minas Gerais (UFMG)
repository.mail.fl_str_mv journal@vet.ufmg.br||abmvz.artigo@abmvz.org.br
_version_ 1750220879214673920