Cellular components and TGF-β1 content of a closed Tube system for Platelet Rich Plasma acquisition in horse]

Detalhes bibliográficos
Autor(a) principal: Fantini,P.
Data de Publicação: 2022
Outros Autores: Jimenez-Aristizabal,R., Iborra,A., Miranda,A.L.S., Maranhão,R.P.A., Leme,F.O.P., Palhares,M.S., Prades,M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Arquivo brasileiro de medicina veterinária e zootecnia (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352022000100093
Resumo: ABSTRACT Platelet-rich plasma (PRP) has been proposed as an agent to accelerate the healing process and stimulate the regenerative capacity of tissues due to its abundance of growth factors. A large variety of kits and protocols are available to obtain PRP by different cell-separation systems. However, the lack of standardization may lead to inconsistent results. The aim of this study was to characterize cellular composition, platelet parameters using the ADVIA 120 flow cytometer, and TGF-β1 concentration from the PRP product obtained through a closed system, using simple centrifugation. Six clinically healthy horses were used in this study. The protocol in the closed system resulted in approximately 1.6-fold higher platelet and approximately 2.0-fold lower white blood cell concentrations in comparison with whole blood values. The evaluated system was efficient in concentrating platelets and in retrieving a small number of leukocytes, using a protocol of single centrifugation at low speed.
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spelling Cellular components and TGF-β1 content of a closed Tube system for Platelet Rich Plasma acquisition in horse]PRPgrowth factorsplatelet concentrateconcentration protocolABSTRACT Platelet-rich plasma (PRP) has been proposed as an agent to accelerate the healing process and stimulate the regenerative capacity of tissues due to its abundance of growth factors. A large variety of kits and protocols are available to obtain PRP by different cell-separation systems. However, the lack of standardization may lead to inconsistent results. The aim of this study was to characterize cellular composition, platelet parameters using the ADVIA 120 flow cytometer, and TGF-β1 concentration from the PRP product obtained through a closed system, using simple centrifugation. Six clinically healthy horses were used in this study. The protocol in the closed system resulted in approximately 1.6-fold higher platelet and approximately 2.0-fold lower white blood cell concentrations in comparison with whole blood values. The evaluated system was efficient in concentrating platelets and in retrieving a small number of leukocytes, using a protocol of single centrifugation at low speed.Universidade Federal de Minas Gerais, Escola de Veterinária2022-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352022000100093Arquivo Brasileiro de Medicina Veterinária e Zootecnia v.74 n.1 2022reponame:Arquivo brasileiro de medicina veterinária e zootecnia (Online)instname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMG10.1590/1678-4162-12257info:eu-repo/semantics/openAccessFantini,P.Jimenez-Aristizabal,R.Iborra,A.Miranda,A.L.S.Maranhão,R.P.A.Leme,F.O.P.Palhares,M.S.Prades,M.eng2022-04-19T00:00:00Zoai:scielo:S0102-09352022000100093Revistahttps://www.scielo.br/j/abmvz/PUBhttps://old.scielo.br/oai/scielo-oai.phpjournal@vet.ufmg.br||abmvz.artigo@abmvz.org.br1678-41620102-0935opendoar:2022-04-19T00:00Arquivo brasileiro de medicina veterinária e zootecnia (Online) - Universidade Federal de Minas Gerais (UFMG)false
dc.title.none.fl_str_mv Cellular components and TGF-β1 content of a closed Tube system for Platelet Rich Plasma acquisition in horse]
title Cellular components and TGF-β1 content of a closed Tube system for Platelet Rich Plasma acquisition in horse]
spellingShingle Cellular components and TGF-β1 content of a closed Tube system for Platelet Rich Plasma acquisition in horse]
Fantini,P.
PRP
growth factors
platelet concentrate
concentration protocol
title_short Cellular components and TGF-β1 content of a closed Tube system for Platelet Rich Plasma acquisition in horse]
title_full Cellular components and TGF-β1 content of a closed Tube system for Platelet Rich Plasma acquisition in horse]
title_fullStr Cellular components and TGF-β1 content of a closed Tube system for Platelet Rich Plasma acquisition in horse]
title_full_unstemmed Cellular components and TGF-β1 content of a closed Tube system for Platelet Rich Plasma acquisition in horse]
title_sort Cellular components and TGF-β1 content of a closed Tube system for Platelet Rich Plasma acquisition in horse]
author Fantini,P.
author_facet Fantini,P.
Jimenez-Aristizabal,R.
Iborra,A.
Miranda,A.L.S.
Maranhão,R.P.A.
Leme,F.O.P.
Palhares,M.S.
Prades,M.
author_role author
author2 Jimenez-Aristizabal,R.
Iborra,A.
Miranda,A.L.S.
Maranhão,R.P.A.
Leme,F.O.P.
Palhares,M.S.
Prades,M.
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Fantini,P.
Jimenez-Aristizabal,R.
Iborra,A.
Miranda,A.L.S.
Maranhão,R.P.A.
Leme,F.O.P.
Palhares,M.S.
Prades,M.
dc.subject.por.fl_str_mv PRP
growth factors
platelet concentrate
concentration protocol
topic PRP
growth factors
platelet concentrate
concentration protocol
description ABSTRACT Platelet-rich plasma (PRP) has been proposed as an agent to accelerate the healing process and stimulate the regenerative capacity of tissues due to its abundance of growth factors. A large variety of kits and protocols are available to obtain PRP by different cell-separation systems. However, the lack of standardization may lead to inconsistent results. The aim of this study was to characterize cellular composition, platelet parameters using the ADVIA 120 flow cytometer, and TGF-β1 concentration from the PRP product obtained through a closed system, using simple centrifugation. Six clinically healthy horses were used in this study. The protocol in the closed system resulted in approximately 1.6-fold higher platelet and approximately 2.0-fold lower white blood cell concentrations in comparison with whole blood values. The evaluated system was efficient in concentrating platelets and in retrieving a small number of leukocytes, using a protocol of single centrifugation at low speed.
publishDate 2022
dc.date.none.fl_str_mv 2022-02-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352022000100093
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352022000100093
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1678-4162-12257
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Universidade Federal de Minas Gerais, Escola de Veterinária
publisher.none.fl_str_mv Universidade Federal de Minas Gerais, Escola de Veterinária
dc.source.none.fl_str_mv Arquivo Brasileiro de Medicina Veterinária e Zootecnia v.74 n.1 2022
reponame:Arquivo brasileiro de medicina veterinária e zootecnia (Online)
instname:Universidade Federal de Minas Gerais (UFMG)
instacron:UFMG
instname_str Universidade Federal de Minas Gerais (UFMG)
instacron_str UFMG
institution UFMG
reponame_str Arquivo brasileiro de medicina veterinária e zootecnia (Online)
collection Arquivo brasileiro de medicina veterinária e zootecnia (Online)
repository.name.fl_str_mv Arquivo brasileiro de medicina veterinária e zootecnia (Online) - Universidade Federal de Minas Gerais (UFMG)
repository.mail.fl_str_mv journal@vet.ufmg.br||abmvz.artigo@abmvz.org.br
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