Chondroitin sulfate and glucosamine in the cartilage and subchondral bone repair of dogs - Histological findings

Detalhes bibliográficos
Autor(a) principal: Eleotério,R.B.
Data de Publicação: 2015
Outros Autores: Pontes,K.C.S., Machado,J.P., Reis,E.C.C., Ferreira,P.S., Silva,M.B., Martins,N.J.S., Fernandes,N.A., Borges,A.P.B.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Arquivo brasileiro de medicina veterinária e zootecnia (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352015000200325
Resumo: Chondroitin and glucosamine sulfate nutraceuticals are commonly used in the management of degenerative articular disease in veterinary routine. However, there are controversies on the contribution of these substances to articular cartilage. The purpose of this study was to evaluate the efficiency of a chondroitin and glucosamine sulfate-based veterinary nutraceutical on the repair of an induced osteochondral defect in a dog femoral condyle, by macroscopic, histological and histomorphometric analyses. The nutraceutical was orally administered the day following injury induction, every 24 hours (treated group, TG, n=24), compared with animals that did not receive the product (control group, CG, n=24). Six animals per group were anaesthetized for sample collection at 15, 30, 60 and 90 days after surgery. At 15 days, defects were macroscopically filled with red-pinkish tissue. After 30 days, whitish color tissue was observed, both in TG and CG animals, with firmer consistency to touch at 60 and 90 postoperative days. Histological analysis demonstrated that, in both groups, there was initial blood clot formation, which was subsequently substituted by a fibrin net, with capillary proliferation from the adjacent bone marrow and infiltration of mesenchymal cells in clot periphery. As cellular differentiation developed, repair tissue presented a fibrocartilage aspect most of the time, and new subchondral bone formation occurred in the deepest area corresponding to the defect. Histomorphometry suggested that the nutraceutical did not favor the articular cartilage repair process. It was concluded that nutraceutical did not significantly influence chondrocytes proliferation or hyaline architecture restoration.
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spelling Chondroitin sulfate and glucosamine in the cartilage and subchondral bone repair of dogs - Histological findingsdegenerative articular diseaseosteoarthritisarthrosischondroprotectivecartilageChondroitin and glucosamine sulfate nutraceuticals are commonly used in the management of degenerative articular disease in veterinary routine. However, there are controversies on the contribution of these substances to articular cartilage. The purpose of this study was to evaluate the efficiency of a chondroitin and glucosamine sulfate-based veterinary nutraceutical on the repair of an induced osteochondral defect in a dog femoral condyle, by macroscopic, histological and histomorphometric analyses. The nutraceutical was orally administered the day following injury induction, every 24 hours (treated group, TG, n=24), compared with animals that did not receive the product (control group, CG, n=24). Six animals per group were anaesthetized for sample collection at 15, 30, 60 and 90 days after surgery. At 15 days, defects were macroscopically filled with red-pinkish tissue. After 30 days, whitish color tissue was observed, both in TG and CG animals, with firmer consistency to touch at 60 and 90 postoperative days. Histological analysis demonstrated that, in both groups, there was initial blood clot formation, which was subsequently substituted by a fibrin net, with capillary proliferation from the adjacent bone marrow and infiltration of mesenchymal cells in clot periphery. As cellular differentiation developed, repair tissue presented a fibrocartilage aspect most of the time, and new subchondral bone formation occurred in the deepest area corresponding to the defect. Histomorphometry suggested that the nutraceutical did not favor the articular cartilage repair process. It was concluded that nutraceutical did not significantly influence chondrocytes proliferation or hyaline architecture restoration.Universidade Federal de Minas Gerais, Escola de Veterinária2015-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352015000200325Arquivo Brasileiro de Medicina Veterinária e Zootecnia v.67 n.2 2015reponame:Arquivo brasileiro de medicina veterinária e zootecnia (Online)instname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMG10.1590/1678-7092info:eu-repo/semantics/openAccessEleotério,R.B.Pontes,K.C.S.Machado,J.P.Reis,E.C.C.Ferreira,P.S.Silva,M.B.Martins,N.J.S.Fernandes,N.A.Borges,A.P.B.eng2015-10-26T00:00:00Zoai:scielo:S0102-09352015000200325Revistahttps://www.scielo.br/j/abmvz/PUBhttps://old.scielo.br/oai/scielo-oai.phpjournal@vet.ufmg.br||abmvz.artigo@abmvz.org.br1678-41620102-0935opendoar:2015-10-26T00:00Arquivo brasileiro de medicina veterinária e zootecnia (Online) - Universidade Federal de Minas Gerais (UFMG)false
dc.title.none.fl_str_mv Chondroitin sulfate and glucosamine in the cartilage and subchondral bone repair of dogs - Histological findings
title Chondroitin sulfate and glucosamine in the cartilage and subchondral bone repair of dogs - Histological findings
spellingShingle Chondroitin sulfate and glucosamine in the cartilage and subchondral bone repair of dogs - Histological findings
Eleotério,R.B.
degenerative articular disease
osteoarthritis
arthrosis
chondroprotective
cartilage
title_short Chondroitin sulfate and glucosamine in the cartilage and subchondral bone repair of dogs - Histological findings
title_full Chondroitin sulfate and glucosamine in the cartilage and subchondral bone repair of dogs - Histological findings
title_fullStr Chondroitin sulfate and glucosamine in the cartilage and subchondral bone repair of dogs - Histological findings
title_full_unstemmed Chondroitin sulfate and glucosamine in the cartilage and subchondral bone repair of dogs - Histological findings
title_sort Chondroitin sulfate and glucosamine in the cartilage and subchondral bone repair of dogs - Histological findings
author Eleotério,R.B.
author_facet Eleotério,R.B.
Pontes,K.C.S.
Machado,J.P.
Reis,E.C.C.
Ferreira,P.S.
Silva,M.B.
Martins,N.J.S.
Fernandes,N.A.
Borges,A.P.B.
author_role author
author2 Pontes,K.C.S.
Machado,J.P.
Reis,E.C.C.
Ferreira,P.S.
Silva,M.B.
Martins,N.J.S.
Fernandes,N.A.
Borges,A.P.B.
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Eleotério,R.B.
Pontes,K.C.S.
Machado,J.P.
Reis,E.C.C.
Ferreira,P.S.
Silva,M.B.
Martins,N.J.S.
Fernandes,N.A.
Borges,A.P.B.
dc.subject.por.fl_str_mv degenerative articular disease
osteoarthritis
arthrosis
chondroprotective
cartilage
topic degenerative articular disease
osteoarthritis
arthrosis
chondroprotective
cartilage
description Chondroitin and glucosamine sulfate nutraceuticals are commonly used in the management of degenerative articular disease in veterinary routine. However, there are controversies on the contribution of these substances to articular cartilage. The purpose of this study was to evaluate the efficiency of a chondroitin and glucosamine sulfate-based veterinary nutraceutical on the repair of an induced osteochondral defect in a dog femoral condyle, by macroscopic, histological and histomorphometric analyses. The nutraceutical was orally administered the day following injury induction, every 24 hours (treated group, TG, n=24), compared with animals that did not receive the product (control group, CG, n=24). Six animals per group were anaesthetized for sample collection at 15, 30, 60 and 90 days after surgery. At 15 days, defects were macroscopically filled with red-pinkish tissue. After 30 days, whitish color tissue was observed, both in TG and CG animals, with firmer consistency to touch at 60 and 90 postoperative days. Histological analysis demonstrated that, in both groups, there was initial blood clot formation, which was subsequently substituted by a fibrin net, with capillary proliferation from the adjacent bone marrow and infiltration of mesenchymal cells in clot periphery. As cellular differentiation developed, repair tissue presented a fibrocartilage aspect most of the time, and new subchondral bone formation occurred in the deepest area corresponding to the defect. Histomorphometry suggested that the nutraceutical did not favor the articular cartilage repair process. It was concluded that nutraceutical did not significantly influence chondrocytes proliferation or hyaline architecture restoration.
publishDate 2015
dc.date.none.fl_str_mv 2015-04-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352015000200325
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352015000200325
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1678-7092
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Universidade Federal de Minas Gerais, Escola de Veterinária
publisher.none.fl_str_mv Universidade Federal de Minas Gerais, Escola de Veterinária
dc.source.none.fl_str_mv Arquivo Brasileiro de Medicina Veterinária e Zootecnia v.67 n.2 2015
reponame:Arquivo brasileiro de medicina veterinária e zootecnia (Online)
instname:Universidade Federal de Minas Gerais (UFMG)
instacron:UFMG
instname_str Universidade Federal de Minas Gerais (UFMG)
instacron_str UFMG
institution UFMG
reponame_str Arquivo brasileiro de medicina veterinária e zootecnia (Online)
collection Arquivo brasileiro de medicina veterinária e zootecnia (Online)
repository.name.fl_str_mv Arquivo brasileiro de medicina veterinária e zootecnia (Online) - Universidade Federal de Minas Gerais (UFMG)
repository.mail.fl_str_mv journal@vet.ufmg.br||abmvz.artigo@abmvz.org.br
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