Chondroitin sulfate and glucosamine in the cartilage and subchondral bone repair of dogs - Histological findings
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Arquivo brasileiro de medicina veterinária e zootecnia (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352015000200325 |
Resumo: | Chondroitin and glucosamine sulfate nutraceuticals are commonly used in the management of degenerative articular disease in veterinary routine. However, there are controversies on the contribution of these substances to articular cartilage. The purpose of this study was to evaluate the efficiency of a chondroitin and glucosamine sulfate-based veterinary nutraceutical on the repair of an induced osteochondral defect in a dog femoral condyle, by macroscopic, histological and histomorphometric analyses. The nutraceutical was orally administered the day following injury induction, every 24 hours (treated group, TG, n=24), compared with animals that did not receive the product (control group, CG, n=24). Six animals per group were anaesthetized for sample collection at 15, 30, 60 and 90 days after surgery. At 15 days, defects were macroscopically filled with red-pinkish tissue. After 30 days, whitish color tissue was observed, both in TG and CG animals, with firmer consistency to touch at 60 and 90 postoperative days. Histological analysis demonstrated that, in both groups, there was initial blood clot formation, which was subsequently substituted by a fibrin net, with capillary proliferation from the adjacent bone marrow and infiltration of mesenchymal cells in clot periphery. As cellular differentiation developed, repair tissue presented a fibrocartilage aspect most of the time, and new subchondral bone formation occurred in the deepest area corresponding to the defect. Histomorphometry suggested that the nutraceutical did not favor the articular cartilage repair process. It was concluded that nutraceutical did not significantly influence chondrocytes proliferation or hyaline architecture restoration. |
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Chondroitin sulfate and glucosamine in the cartilage and subchondral bone repair of dogs - Histological findingsdegenerative articular diseaseosteoarthritisarthrosischondroprotectivecartilageChondroitin and glucosamine sulfate nutraceuticals are commonly used in the management of degenerative articular disease in veterinary routine. However, there are controversies on the contribution of these substances to articular cartilage. The purpose of this study was to evaluate the efficiency of a chondroitin and glucosamine sulfate-based veterinary nutraceutical on the repair of an induced osteochondral defect in a dog femoral condyle, by macroscopic, histological and histomorphometric analyses. The nutraceutical was orally administered the day following injury induction, every 24 hours (treated group, TG, n=24), compared with animals that did not receive the product (control group, CG, n=24). Six animals per group were anaesthetized for sample collection at 15, 30, 60 and 90 days after surgery. At 15 days, defects were macroscopically filled with red-pinkish tissue. After 30 days, whitish color tissue was observed, both in TG and CG animals, with firmer consistency to touch at 60 and 90 postoperative days. Histological analysis demonstrated that, in both groups, there was initial blood clot formation, which was subsequently substituted by a fibrin net, with capillary proliferation from the adjacent bone marrow and infiltration of mesenchymal cells in clot periphery. As cellular differentiation developed, repair tissue presented a fibrocartilage aspect most of the time, and new subchondral bone formation occurred in the deepest area corresponding to the defect. Histomorphometry suggested that the nutraceutical did not favor the articular cartilage repair process. It was concluded that nutraceutical did not significantly influence chondrocytes proliferation or hyaline architecture restoration.Universidade Federal de Minas Gerais, Escola de Veterinária2015-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352015000200325Arquivo Brasileiro de Medicina Veterinária e Zootecnia v.67 n.2 2015reponame:Arquivo brasileiro de medicina veterinária e zootecnia (Online)instname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMG10.1590/1678-7092info:eu-repo/semantics/openAccessEleotério,R.B.Pontes,K.C.S.Machado,J.P.Reis,E.C.C.Ferreira,P.S.Silva,M.B.Martins,N.J.S.Fernandes,N.A.Borges,A.P.B.eng2015-10-26T00:00:00Zoai:scielo:S0102-09352015000200325Revistahttps://www.scielo.br/j/abmvz/PUBhttps://old.scielo.br/oai/scielo-oai.phpjournal@vet.ufmg.br||abmvz.artigo@abmvz.org.br1678-41620102-0935opendoar:2015-10-26T00:00Arquivo brasileiro de medicina veterinária e zootecnia (Online) - Universidade Federal de Minas Gerais (UFMG)false |
dc.title.none.fl_str_mv |
Chondroitin sulfate and glucosamine in the cartilage and subchondral bone repair of dogs - Histological findings |
title |
Chondroitin sulfate and glucosamine in the cartilage and subchondral bone repair of dogs - Histological findings |
spellingShingle |
Chondroitin sulfate and glucosamine in the cartilage and subchondral bone repair of dogs - Histological findings Eleotério,R.B. degenerative articular disease osteoarthritis arthrosis chondroprotective cartilage |
title_short |
Chondroitin sulfate and glucosamine in the cartilage and subchondral bone repair of dogs - Histological findings |
title_full |
Chondroitin sulfate and glucosamine in the cartilage and subchondral bone repair of dogs - Histological findings |
title_fullStr |
Chondroitin sulfate and glucosamine in the cartilage and subchondral bone repair of dogs - Histological findings |
title_full_unstemmed |
Chondroitin sulfate and glucosamine in the cartilage and subchondral bone repair of dogs - Histological findings |
title_sort |
Chondroitin sulfate and glucosamine in the cartilage and subchondral bone repair of dogs - Histological findings |
author |
Eleotério,R.B. |
author_facet |
Eleotério,R.B. Pontes,K.C.S. Machado,J.P. Reis,E.C.C. Ferreira,P.S. Silva,M.B. Martins,N.J.S. Fernandes,N.A. Borges,A.P.B. |
author_role |
author |
author2 |
Pontes,K.C.S. Machado,J.P. Reis,E.C.C. Ferreira,P.S. Silva,M.B. Martins,N.J.S. Fernandes,N.A. Borges,A.P.B. |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Eleotério,R.B. Pontes,K.C.S. Machado,J.P. Reis,E.C.C. Ferreira,P.S. Silva,M.B. Martins,N.J.S. Fernandes,N.A. Borges,A.P.B. |
dc.subject.por.fl_str_mv |
degenerative articular disease osteoarthritis arthrosis chondroprotective cartilage |
topic |
degenerative articular disease osteoarthritis arthrosis chondroprotective cartilage |
description |
Chondroitin and glucosamine sulfate nutraceuticals are commonly used in the management of degenerative articular disease in veterinary routine. However, there are controversies on the contribution of these substances to articular cartilage. The purpose of this study was to evaluate the efficiency of a chondroitin and glucosamine sulfate-based veterinary nutraceutical on the repair of an induced osteochondral defect in a dog femoral condyle, by macroscopic, histological and histomorphometric analyses. The nutraceutical was orally administered the day following injury induction, every 24 hours (treated group, TG, n=24), compared with animals that did not receive the product (control group, CG, n=24). Six animals per group were anaesthetized for sample collection at 15, 30, 60 and 90 days after surgery. At 15 days, defects were macroscopically filled with red-pinkish tissue. After 30 days, whitish color tissue was observed, both in TG and CG animals, with firmer consistency to touch at 60 and 90 postoperative days. Histological analysis demonstrated that, in both groups, there was initial blood clot formation, which was subsequently substituted by a fibrin net, with capillary proliferation from the adjacent bone marrow and infiltration of mesenchymal cells in clot periphery. As cellular differentiation developed, repair tissue presented a fibrocartilage aspect most of the time, and new subchondral bone formation occurred in the deepest area corresponding to the defect. Histomorphometry suggested that the nutraceutical did not favor the articular cartilage repair process. It was concluded that nutraceutical did not significantly influence chondrocytes proliferation or hyaline architecture restoration. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-04-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352015000200325 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352015000200325 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1678-7092 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Universidade Federal de Minas Gerais, Escola de Veterinária |
publisher.none.fl_str_mv |
Universidade Federal de Minas Gerais, Escola de Veterinária |
dc.source.none.fl_str_mv |
Arquivo Brasileiro de Medicina Veterinária e Zootecnia v.67 n.2 2015 reponame:Arquivo brasileiro de medicina veterinária e zootecnia (Online) instname:Universidade Federal de Minas Gerais (UFMG) instacron:UFMG |
instname_str |
Universidade Federal de Minas Gerais (UFMG) |
instacron_str |
UFMG |
institution |
UFMG |
reponame_str |
Arquivo brasileiro de medicina veterinária e zootecnia (Online) |
collection |
Arquivo brasileiro de medicina veterinária e zootecnia (Online) |
repository.name.fl_str_mv |
Arquivo brasileiro de medicina veterinária e zootecnia (Online) - Universidade Federal de Minas Gerais (UFMG) |
repository.mail.fl_str_mv |
journal@vet.ufmg.br||abmvz.artigo@abmvz.org.br |
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1750220888569020416 |