Stability and toxicity of Clostridium perfringens type D epsilon prototoxin treated by iodine

Detalhes bibliográficos
Autor(a) principal: Rocha,P.H.
Data de Publicação: 2008
Outros Autores: Assis,R.A., Lobato,F.C.F., Cardoso,V.N., Heneine,L.G.D.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Arquivo brasileiro de medicina veterinária e zootecnia (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000400007
Resumo: Purified epsilon prototoxin of Clostridium perfringens type D was produced, purified, and detoxified by the stoiechiometric method of non-radioactive iodine incorporation. Different degrees of iodination were perfomed and the toxicity of the derivatives were analysed by in vivo studies. Toxicity decreased inversely to the iodine incorporation. Eletrophoretic analysis showed different levels of stability of samples kept under different temperatures 4ºC, - 20ºC, and -80 ºC. The iodinated prototoxins were stocked for a period of four months.
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spelling Stability and toxicity of Clostridium perfringens type D epsilon prototoxin treated by iodineClostridium perfringensepsilon toxiniodinationstabilityPurified epsilon prototoxin of Clostridium perfringens type D was produced, purified, and detoxified by the stoiechiometric method of non-radioactive iodine incorporation. Different degrees of iodination were perfomed and the toxicity of the derivatives were analysed by in vivo studies. Toxicity decreased inversely to the iodine incorporation. Eletrophoretic analysis showed different levels of stability of samples kept under different temperatures 4ºC, - 20ºC, and -80 ºC. The iodinated prototoxins were stocked for a period of four months.Universidade Federal de Minas Gerais, Escola de Veterinária2008-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000400007Arquivo Brasileiro de Medicina Veterinária e Zootecnia v.60 n.4 2008reponame:Arquivo brasileiro de medicina veterinária e zootecnia (Online)instname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMG10.1590/S0102-09352008000400007info:eu-repo/semantics/openAccessRocha,P.H.Assis,R.A.Lobato,F.C.F.Cardoso,V.N.Heneine,L.G.D.eng2008-08-15T00:00:00Zoai:scielo:S0102-09352008000400007Revistahttps://www.scielo.br/j/abmvz/PUBhttps://old.scielo.br/oai/scielo-oai.phpjournal@vet.ufmg.br||abmvz.artigo@abmvz.org.br1678-41620102-0935opendoar:2008-08-15T00:00Arquivo brasileiro de medicina veterinária e zootecnia (Online) - Universidade Federal de Minas Gerais (UFMG)false
dc.title.none.fl_str_mv Stability and toxicity of Clostridium perfringens type D epsilon prototoxin treated by iodine
title Stability and toxicity of Clostridium perfringens type D epsilon prototoxin treated by iodine
spellingShingle Stability and toxicity of Clostridium perfringens type D epsilon prototoxin treated by iodine
Rocha,P.H.
Clostridium perfringens
epsilon toxin
iodination
stability
title_short Stability and toxicity of Clostridium perfringens type D epsilon prototoxin treated by iodine
title_full Stability and toxicity of Clostridium perfringens type D epsilon prototoxin treated by iodine
title_fullStr Stability and toxicity of Clostridium perfringens type D epsilon prototoxin treated by iodine
title_full_unstemmed Stability and toxicity of Clostridium perfringens type D epsilon prototoxin treated by iodine
title_sort Stability and toxicity of Clostridium perfringens type D epsilon prototoxin treated by iodine
author Rocha,P.H.
author_facet Rocha,P.H.
Assis,R.A.
Lobato,F.C.F.
Cardoso,V.N.
Heneine,L.G.D.
author_role author
author2 Assis,R.A.
Lobato,F.C.F.
Cardoso,V.N.
Heneine,L.G.D.
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Rocha,P.H.
Assis,R.A.
Lobato,F.C.F.
Cardoso,V.N.
Heneine,L.G.D.
dc.subject.por.fl_str_mv Clostridium perfringens
epsilon toxin
iodination
stability
topic Clostridium perfringens
epsilon toxin
iodination
stability
description Purified epsilon prototoxin of Clostridium perfringens type D was produced, purified, and detoxified by the stoiechiometric method of non-radioactive iodine incorporation. Different degrees of iodination were perfomed and the toxicity of the derivatives were analysed by in vivo studies. Toxicity decreased inversely to the iodine incorporation. Eletrophoretic analysis showed different levels of stability of samples kept under different temperatures 4ºC, - 20ºC, and -80 ºC. The iodinated prototoxins were stocked for a period of four months.
publishDate 2008
dc.date.none.fl_str_mv 2008-08-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000400007
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000400007
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0102-09352008000400007
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Universidade Federal de Minas Gerais, Escola de Veterinária
publisher.none.fl_str_mv Universidade Federal de Minas Gerais, Escola de Veterinária
dc.source.none.fl_str_mv Arquivo Brasileiro de Medicina Veterinária e Zootecnia v.60 n.4 2008
reponame:Arquivo brasileiro de medicina veterinária e zootecnia (Online)
instname:Universidade Federal de Minas Gerais (UFMG)
instacron:UFMG
instname_str Universidade Federal de Minas Gerais (UFMG)
instacron_str UFMG
institution UFMG
reponame_str Arquivo brasileiro de medicina veterinária e zootecnia (Online)
collection Arquivo brasileiro de medicina veterinária e zootecnia (Online)
repository.name.fl_str_mv Arquivo brasileiro de medicina veterinária e zootecnia (Online) - Universidade Federal de Minas Gerais (UFMG)
repository.mail.fl_str_mv journal@vet.ufmg.br||abmvz.artigo@abmvz.org.br
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