In vitro evaluation of Clostridium septicum alpha toxoid

Detalhes bibliográficos
Autor(a) principal: Salvarani,F.M.
Data de Publicação: 2010
Outros Autores: Lobato,Z.I.P., Assis,R.A., Lima,C.G.R.D., Silva,R.O.S., Pires,P.S., Lobato,F.C.F.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Arquivo brasileiro de medicina veterinária e zootecnia (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352010000400002
Resumo: Aiming to investigate in vitro alternatives, a test for neutralizing antibody detection using cell culture was developed. This test was more sensitive than previous animal models, allowing for detection of substantially lower alpha toxin and anti-alpha toxin titers. Titers observed during in vivo and in vitro seroneutralization had a correlation of 99.12%, indicating that cell culture is a viable alternative in the evaluation of vaccine potency, screening of vaccinal seeds, and Clostridium septicum alpha toxin titration.
id UFMG-8_fd4344480476449acf1c1af7c6dea63b
oai_identifier_str oai:scielo:S0102-09352010000400002
network_acronym_str UFMG-8
network_name_str Arquivo brasileiro de medicina veterinária e zootecnia (Online)
repository_id_str
spelling In vitro evaluation of Clostridium septicum alpha toxoidseroneutralizationmouseguinea pigcell culturevaccineAiming to investigate in vitro alternatives, a test for neutralizing antibody detection using cell culture was developed. This test was more sensitive than previous animal models, allowing for detection of substantially lower alpha toxin and anti-alpha toxin titers. Titers observed during in vivo and in vitro seroneutralization had a correlation of 99.12%, indicating that cell culture is a viable alternative in the evaluation of vaccine potency, screening of vaccinal seeds, and Clostridium septicum alpha toxin titration.Universidade Federal de Minas Gerais, Escola de Veterinária2010-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352010000400002Arquivo Brasileiro de Medicina Veterinária e Zootecnia v.62 n.4 2010reponame:Arquivo brasileiro de medicina veterinária e zootecnia (Online)instname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMG10.1590/S0102-09352010000400002info:eu-repo/semantics/openAccessSalvarani,F.M.Lobato,Z.I.P.Assis,R.A.Lima,C.G.R.D.Silva,R.O.S.Pires,P.S.Lobato,F.C.F.eng2011-02-02T00:00:00Zoai:scielo:S0102-09352010000400002Revistahttps://www.scielo.br/j/abmvz/PUBhttps://old.scielo.br/oai/scielo-oai.phpjournal@vet.ufmg.br||abmvz.artigo@abmvz.org.br1678-41620102-0935opendoar:2011-02-02T00:00Arquivo brasileiro de medicina veterinária e zootecnia (Online) - Universidade Federal de Minas Gerais (UFMG)false
dc.title.none.fl_str_mv In vitro evaluation of Clostridium septicum alpha toxoid
title In vitro evaluation of Clostridium septicum alpha toxoid
spellingShingle In vitro evaluation of Clostridium septicum alpha toxoid
Salvarani,F.M.
seroneutralization
mouse
guinea pig
cell culture
vaccine
title_short In vitro evaluation of Clostridium septicum alpha toxoid
title_full In vitro evaluation of Clostridium septicum alpha toxoid
title_fullStr In vitro evaluation of Clostridium septicum alpha toxoid
title_full_unstemmed In vitro evaluation of Clostridium septicum alpha toxoid
title_sort In vitro evaluation of Clostridium septicum alpha toxoid
author Salvarani,F.M.
author_facet Salvarani,F.M.
Lobato,Z.I.P.
Assis,R.A.
Lima,C.G.R.D.
Silva,R.O.S.
Pires,P.S.
Lobato,F.C.F.
author_role author
author2 Lobato,Z.I.P.
Assis,R.A.
Lima,C.G.R.D.
Silva,R.O.S.
Pires,P.S.
Lobato,F.C.F.
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Salvarani,F.M.
Lobato,Z.I.P.
Assis,R.A.
Lima,C.G.R.D.
Silva,R.O.S.
Pires,P.S.
Lobato,F.C.F.
dc.subject.por.fl_str_mv seroneutralization
mouse
guinea pig
cell culture
vaccine
topic seroneutralization
mouse
guinea pig
cell culture
vaccine
description Aiming to investigate in vitro alternatives, a test for neutralizing antibody detection using cell culture was developed. This test was more sensitive than previous animal models, allowing for detection of substantially lower alpha toxin and anti-alpha toxin titers. Titers observed during in vivo and in vitro seroneutralization had a correlation of 99.12%, indicating that cell culture is a viable alternative in the evaluation of vaccine potency, screening of vaccinal seeds, and Clostridium septicum alpha toxin titration.
publishDate 2010
dc.date.none.fl_str_mv 2010-08-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352010000400002
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352010000400002
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0102-09352010000400002
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Universidade Federal de Minas Gerais, Escola de Veterinária
publisher.none.fl_str_mv Universidade Federal de Minas Gerais, Escola de Veterinária
dc.source.none.fl_str_mv Arquivo Brasileiro de Medicina Veterinária e Zootecnia v.62 n.4 2010
reponame:Arquivo brasileiro de medicina veterinária e zootecnia (Online)
instname:Universidade Federal de Minas Gerais (UFMG)
instacron:UFMG
instname_str Universidade Federal de Minas Gerais (UFMG)
instacron_str UFMG
institution UFMG
reponame_str Arquivo brasileiro de medicina veterinária e zootecnia (Online)
collection Arquivo brasileiro de medicina veterinária e zootecnia (Online)
repository.name.fl_str_mv Arquivo brasileiro de medicina veterinária e zootecnia (Online) - Universidade Federal de Minas Gerais (UFMG)
repository.mail.fl_str_mv journal@vet.ufmg.br||abmvz.artigo@abmvz.org.br
_version_ 1750220883660636160

Registros relacionados