Calix[n]arene-based immunogens: a new non-proteic strategy for anti-cocaine vaccine
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2022 |
| Outros Autores: | , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UFMG |
| Texto Completo: | https://doi.org/10.1016/j.jare.2021.09.003 http://hdl.handle.net/1843/51816 |
Resumo: | CNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico |
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2023-04-11T20:37:15Z2023-04-11T20:37:15Z2022-0538285298https://doi.org/10.1016/j.jare.2021.09.00320901232http://hdl.handle.net/1843/51816CNPq - Conselho Nacional de Desenvolvimento Científico e TecnológicoFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas GeraisCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível SuperiorIntroduction Cocaine use disorder is a significant public health issue without a current specific approved treatment. Among different approaches to this disorder, it is possible to highlight a promising immunologic strategy in which an immunogenic agent may reduce the reinforcing effects of the drug if they are able to yield sufficient specific antibodies capable to bind cocaine and/or its psychoactive metabolites before entering into the brain. Several carriers have been investigated in the anti-cocaine vaccine development; however, they generally present a very complex chemical structure, which potentially hampers the proper assessment of the coupling efficiency between the hapten units and the protein structure. Objectives The present study reports the design, synthesis and preclinical evaluation of two novel calix[n]arene-based anti-cocaine immunogens (herein named as V4N2 and V8N2) by the tethering of the hydrolysis-tolerant hapten GNE (15) on calix[4]arene and calix[8]arene moieties. Methods The preclinical assessment corresponded to the immunogenicity and dose–response evaluation of V4N2 and V8N2. The potential of the produced antibodies to reduce the passage of cocaine analogue through the blood–brain-barrier (BBB), modifying its biodistribution was also investigated. Results Both calix[n]arene-based immunogens elicited high titers of cocaine antibodies that modified the biodistribution of a cocaine radiolabeled analogue (99mTc-TRODAT-1) and decreased cocaine-induced behavior, according to an animal model. Conclusion The present results demonstrate the potential of V4N2 and V8N2 as immunogens for the treatment of cocaine use disorder.engUniversidade Federal de Minas GeraisUFMGBrasilFAR - DEPARTAMENTO DE ALIMENTOSFAR - DEPARTAMENTO DE ANÁLISES CLÍNICAS E TOXICOLÓGICASFAR - DEPARTAMENTO DE PRODUTOS FARMACÊUTICOSMED - DEPARTAMENTO DE SAÚDE MENTALJournal of Advanced ResearchCocaínaDependência químicaImunoterapiaCocaineCrackChemical addictionCalixarenesImmunotherapyTRODAT-1Calix[n]arene-based immunogens: a new non-proteic strategy for anti-cocaine vaccineinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttps://www.sciencedirect.com/science/article/pii/S2090123221001715Leonardo da Silva NetoAngélica Faleiros da Silva MaiaAdriana Martins GodinPaulo Sérgio de Almeida AugustoRaissa Lima Gonçalves PereiraSordaini Maria CaligiorneRosemeire Brondi AlvesSimone Odília Antunes FernandesValbert Nascimento CardosoGisele Assis Castro GoulartFelipe Terra MartinsMaila de Castro Lourenço das NevesFrederico Duarte GarciaÂngelo de Fátimaapplication/pdfinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMGinstname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMGLICENSELicense.txtLicense.txttext/plain; charset=utf-82042https://repositorio.ufmg.br/bitstream/1843/51816/1/License.txtfa505098d172de0bc8864fc1287ffe22MD51ORIGINALCalix[n]arene-based immunogens A new non-proteic strategy for anti-cocaine vaccine.pdfCalix[n]arene-based immunogens A new non-proteic strategy for anti-cocaine vaccine.pdfapplication/pdf2328970https://repositorio.ufmg.br/bitstream/1843/51816/2/Calix%5bn%5darene-based%20immunogens%20A%20new%20non-proteic%20strategy%20for%20anti-cocaine%20vaccine.pdf6a5608ee97d52fc0ec5dc1ae99a084fdMD521843/518162023-04-11 21:54:36.489oai:repositorio.ufmg.br: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Repositório de PublicaçõesPUBhttps://repositorio.ufmg.br/oaiopendoar:2023-04-12T00:54:36Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)false |
| dc.title.pt_BR.fl_str_mv |
Calix[n]arene-based immunogens: a new non-proteic strategy for anti-cocaine vaccine |
| title |
Calix[n]arene-based immunogens: a new non-proteic strategy for anti-cocaine vaccine |
| spellingShingle |
Calix[n]arene-based immunogens: a new non-proteic strategy for anti-cocaine vaccine Leonardo da Silva Neto Cocaine Crack Chemical addiction Calixarenes Immunotherapy TRODAT-1 Cocaína Dependência química Imunoterapia |
| title_short |
Calix[n]arene-based immunogens: a new non-proteic strategy for anti-cocaine vaccine |
| title_full |
Calix[n]arene-based immunogens: a new non-proteic strategy for anti-cocaine vaccine |
| title_fullStr |
Calix[n]arene-based immunogens: a new non-proteic strategy for anti-cocaine vaccine |
| title_full_unstemmed |
Calix[n]arene-based immunogens: a new non-proteic strategy for anti-cocaine vaccine |
| title_sort |
Calix[n]arene-based immunogens: a new non-proteic strategy for anti-cocaine vaccine |
| author |
Leonardo da Silva Neto |
| author_facet |
Leonardo da Silva Neto Angélica Faleiros da Silva Maia Adriana Martins Godin Paulo Sérgio de Almeida Augusto Raissa Lima Gonçalves Pereira Sordaini Maria Caligiorne Rosemeire Brondi Alves Simone Odília Antunes Fernandes Valbert Nascimento Cardoso Gisele Assis Castro Goulart Felipe Terra Martins Maila de Castro Lourenço das Neves Frederico Duarte Garcia Ângelo de Fátima |
| author_role |
author |
| author2 |
Angélica Faleiros da Silva Maia Adriana Martins Godin Paulo Sérgio de Almeida Augusto Raissa Lima Gonçalves Pereira Sordaini Maria Caligiorne Rosemeire Brondi Alves Simone Odília Antunes Fernandes Valbert Nascimento Cardoso Gisele Assis Castro Goulart Felipe Terra Martins Maila de Castro Lourenço das Neves Frederico Duarte Garcia Ângelo de Fátima |
| author2_role |
author author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Leonardo da Silva Neto Angélica Faleiros da Silva Maia Adriana Martins Godin Paulo Sérgio de Almeida Augusto Raissa Lima Gonçalves Pereira Sordaini Maria Caligiorne Rosemeire Brondi Alves Simone Odília Antunes Fernandes Valbert Nascimento Cardoso Gisele Assis Castro Goulart Felipe Terra Martins Maila de Castro Lourenço das Neves Frederico Duarte Garcia Ângelo de Fátima |
| dc.subject.por.fl_str_mv |
Cocaine Crack Chemical addiction Calixarenes Immunotherapy TRODAT-1 |
| topic |
Cocaine Crack Chemical addiction Calixarenes Immunotherapy TRODAT-1 Cocaína Dependência química Imunoterapia |
| dc.subject.other.pt_BR.fl_str_mv |
Cocaína Dependência química Imunoterapia |
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CNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico |
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2022 |
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2022-05 |
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2023-04-11T20:37:15Z |
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2023-04-11T20:37:15Z |
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info:eu-repo/semantics/publishedVersion |
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http://hdl.handle.net/1843/51816 |
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https://doi.org/10.1016/j.jare.2021.09.003 |
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20901232 |
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https://doi.org/10.1016/j.jare.2021.09.003 http://hdl.handle.net/1843/51816 |
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eng |
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Journal of Advanced Research |
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openAccess |
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Universidade Federal de Minas Gerais |
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Brasil |
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Universidade Federal de Minas Gerais |
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