The life cycle of a zoonotic parasite reassessed: experimental infection of Melanoides tuberculata (Mollusca: Thiaridae) with Centrocestus formosanus (Trematoda: Heterophyidae)
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFMG |
Texto Completo: | https://doi.org/10.1371/journal.pone.0194161 http://hdl.handle.net/1843/56290 https://orcid.org/0000-0002-5519-1348 https://orcid.org/0000-0001-8799-4885 https://orcid.org/0000-0002-9039-3068 https://orcid.org/0000-0001-7118-7210 |
Resumo: | Centrocestus formosanus is a foodborne intestinal trematode that is native to Asia and has been introduced into the Americas and Europe. Although there are several studies of C. formosanus in definitive vertebrate hosts (birds and mammals, including humans), and in intermediate vertebrate hosts (fish and amphibians), there is little published information regarding interaction with its transmitting mollusc. In this study we studied the miracidial development of C. formosanus using a mouse as a source of eggs. Adult parasites were maintained in water in order to develop miracidia in intrauterine eggs. Miracidia appeared at 12 days of incubation, with no hatching observed for up to 40 days. Subsequently, we placed dead C. formosanus containing eggs with miracidia individually in contact with 48 specimens of Melanoides tuberculata, and observed the absence of the parasites after 1h of exposure, suggesting that they were ingested by the snails. Of the 33 experimentally-infected snails that were alive after 84–89 days post-infection (DPI), seven (21%) shed cercariae. We detected young C. formosanus rediae in 21/33 (64%) M. tuberculata at 90 DPI. To our knowledge, this report is the first to show that, in the life cycle of C. formosanus, infection of molluscs occurs passively by ingestion of eggs, followed by a long intramolluscan phase. We compare these data with those described for other Heterophyidae, and discuss on the phylogenetic background of the pattern of miracidial development verified in these parasites. |
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2023-07-14T20:27:02Z2023-07-14T20:27:02Z2018134https://doi.org/10.1371/journal.pone.01941611932-6203http://hdl.handle.net/1843/56290https://orcid.org/0000-0002-5519-1348https://orcid.org/0000-0001-8799-4885https://orcid.org/0000-0002-9039-3068https://orcid.org/0000-0001-7118-7210Centrocestus formosanus is a foodborne intestinal trematode that is native to Asia and has been introduced into the Americas and Europe. Although there are several studies of C. formosanus in definitive vertebrate hosts (birds and mammals, including humans), and in intermediate vertebrate hosts (fish and amphibians), there is little published information regarding interaction with its transmitting mollusc. In this study we studied the miracidial development of C. formosanus using a mouse as a source of eggs. Adult parasites were maintained in water in order to develop miracidia in intrauterine eggs. Miracidia appeared at 12 days of incubation, with no hatching observed for up to 40 days. Subsequently, we placed dead C. formosanus containing eggs with miracidia individually in contact with 48 specimens of Melanoides tuberculata, and observed the absence of the parasites after 1h of exposure, suggesting that they were ingested by the snails. Of the 33 experimentally-infected snails that were alive after 84–89 days post-infection (DPI), seven (21%) shed cercariae. We detected young C. formosanus rediae in 21/33 (64%) M. tuberculata at 90 DPI. To our knowledge, this report is the first to show that, in the life cycle of C. formosanus, infection of molluscs occurs passively by ingestion of eggs, followed by a long intramolluscan phase. We compare these data with those described for other Heterophyidae, and discuss on the phylogenetic background of the pattern of miracidial development verified in these parasites.O Centrocestus formosanus é um trematódeo intestinal de origem alimentar, nativo da Ásia e introduzido nas Américas e na Europa. Embora existam vários estudos de C. formosanus em hospedeiros vertebrados definitivos (aves e mamíferos, incluindo humanos) e em hospedeiros vertebrados intermediários (peixes e anfíbios), há poucas informações publicadas sobre a interação com seu molusco transmissor. Neste estudo estudamos o desenvolvimento miracidial de C. formosanus usando um camundongo como fonte de ovos. Parasitos adultos foram mantidos em água para desenvolver miracídios em ovos intra-uterinos. Os miracídios apareceram aos 12 dias de incubação, sem eclosão observada por até 40 dias. Posteriormente, colocamos C. formosanus mortos contendo ovos com miracídios individualmente em contato com 48 espécimes de Melanoides tuberculata e observamos a ausência dos parasitas após 1h de exposição, sugerindo que eles foram ingeridos pelos caramujos. Dos 33 caramujos infectados experimentalmente que estavam vivos após 84-89 dias pós-infecção (DPI), sete (21%) eliminaram cercárias. Detectamos C. formosanus rediae jovem em 21/33 (64%) M. tuberculata aos 90 DPI. Ao nosso conhecimento, este relato é o primeiro a mostrar que, no ciclo de vida de C. formosanus, a infecção de moluscos ocorre passivamente pela ingestão de ovos, seguida por uma longa fase intramolluscan. Comparamos estes dados com aqueles descritos para outros Heterophyidae, e discutimos sobre o background filogenético do padrão de desenvolvimento miracidial verificado nestes parasitos.CNPq - Conselho Nacional de Desenvolvimento Científico e TecnológicoFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas GeraisengUniversidade Federal de Minas GeraisUFMGBrasilICB - DEPARTAMENTO DE PARASITOLOGIAPLoS OneMoluscosHeterophyidaeMelanoides tuberculataCentrocestus formosanusThe life cycle of a zoonotic parasite reassessed: experimental infection of Melanoides tuberculata (Mollusca: Thiaridae) with Centrocestus formosanus (Trematoda: Heterophyidae)O ciclo de vida de um parasita zoonótico reavaliado: Infecção experimental de Melanoides tuberculata (Mollusca: Thiaridae) com Centrocestus formosanus (Trematoda: Heterophyidae)info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0194161Hudson Alves PintoNicole Quaresma GonçalvesDanimar Danilia López HernándezEduardo Alberto Pulido MurilloAlan Lane de Meloapplication/pdfinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMGinstname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMGLICENSELicense.txtLicense.txttext/plain; charset=utf-82042https://repositorio.ufmg.br/bitstream/1843/56290/1/License.txtfa505098d172de0bc8864fc1287ffe22MD51ORIGINALThe life cycle of a zoonotic parasite reassessed_ Experimental infection of Melanoides tuberculata (Mollusca_ Thiaridae) with Centrocestus formosanus (Trematoda_ Heterophyidae).pdfThe life cycle of a zoonotic parasite reassessed_ Experimental infection of Melanoides tuberculata (Mollusca_ Thiaridae) with Centrocestus formosanus (Trematoda_ Heterophyidae).pdfapplication/pdf1517686https://repositorio.ufmg.br/bitstream/1843/56290/2/The%20life%20cycle%20of%20a%20zoonotic%20parasite%20reassessed_%20Experimental%20infection%20of%20Melanoides%20tuberculata%20%28Mollusca_%20Thiaridae%29%20with%20Centrocestus%20formosanus%20%28Trematoda_%20Heterophyidae%29.pdf163ae0508b39e351832a05f0dccc6379MD521843/562902023-07-14 17:27:02.3oai:repositorio.ufmg.br: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Repositório de PublicaçõesPUBhttps://repositorio.ufmg.br/oaiopendoar:2023-07-14T20:27:02Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)false |
dc.title.pt_BR.fl_str_mv |
The life cycle of a zoonotic parasite reassessed: experimental infection of Melanoides tuberculata (Mollusca: Thiaridae) with Centrocestus formosanus (Trematoda: Heterophyidae) |
dc.title.alternative.pt_BR.fl_str_mv |
O ciclo de vida de um parasita zoonótico reavaliado: Infecção experimental de Melanoides tuberculata (Mollusca: Thiaridae) com Centrocestus formosanus (Trematoda: Heterophyidae) |
title |
The life cycle of a zoonotic parasite reassessed: experimental infection of Melanoides tuberculata (Mollusca: Thiaridae) with Centrocestus formosanus (Trematoda: Heterophyidae) |
spellingShingle |
The life cycle of a zoonotic parasite reassessed: experimental infection of Melanoides tuberculata (Mollusca: Thiaridae) with Centrocestus formosanus (Trematoda: Heterophyidae) Hudson Alves Pinto Melanoides tuberculata Centrocestus formosanus Moluscos Heterophyidae |
title_short |
The life cycle of a zoonotic parasite reassessed: experimental infection of Melanoides tuberculata (Mollusca: Thiaridae) with Centrocestus formosanus (Trematoda: Heterophyidae) |
title_full |
The life cycle of a zoonotic parasite reassessed: experimental infection of Melanoides tuberculata (Mollusca: Thiaridae) with Centrocestus formosanus (Trematoda: Heterophyidae) |
title_fullStr |
The life cycle of a zoonotic parasite reassessed: experimental infection of Melanoides tuberculata (Mollusca: Thiaridae) with Centrocestus formosanus (Trematoda: Heterophyidae) |
title_full_unstemmed |
The life cycle of a zoonotic parasite reassessed: experimental infection of Melanoides tuberculata (Mollusca: Thiaridae) with Centrocestus formosanus (Trematoda: Heterophyidae) |
title_sort |
The life cycle of a zoonotic parasite reassessed: experimental infection of Melanoides tuberculata (Mollusca: Thiaridae) with Centrocestus formosanus (Trematoda: Heterophyidae) |
author |
Hudson Alves Pinto |
author_facet |
Hudson Alves Pinto Nicole Quaresma Gonçalves Danimar Danilia López Hernández Eduardo Alberto Pulido Murillo Alan Lane de Melo |
author_role |
author |
author2 |
Nicole Quaresma Gonçalves Danimar Danilia López Hernández Eduardo Alberto Pulido Murillo Alan Lane de Melo |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Hudson Alves Pinto Nicole Quaresma Gonçalves Danimar Danilia López Hernández Eduardo Alberto Pulido Murillo Alan Lane de Melo |
dc.subject.por.fl_str_mv |
Melanoides tuberculata Centrocestus formosanus |
topic |
Melanoides tuberculata Centrocestus formosanus Moluscos Heterophyidae |
dc.subject.other.pt_BR.fl_str_mv |
Moluscos Heterophyidae |
description |
Centrocestus formosanus is a foodborne intestinal trematode that is native to Asia and has been introduced into the Americas and Europe. Although there are several studies of C. formosanus in definitive vertebrate hosts (birds and mammals, including humans), and in intermediate vertebrate hosts (fish and amphibians), there is little published information regarding interaction with its transmitting mollusc. In this study we studied the miracidial development of C. formosanus using a mouse as a source of eggs. Adult parasites were maintained in water in order to develop miracidia in intrauterine eggs. Miracidia appeared at 12 days of incubation, with no hatching observed for up to 40 days. Subsequently, we placed dead C. formosanus containing eggs with miracidia individually in contact with 48 specimens of Melanoides tuberculata, and observed the absence of the parasites after 1h of exposure, suggesting that they were ingested by the snails. Of the 33 experimentally-infected snails that were alive after 84–89 days post-infection (DPI), seven (21%) shed cercariae. We detected young C. formosanus rediae in 21/33 (64%) M. tuberculata at 90 DPI. To our knowledge, this report is the first to show that, in the life cycle of C. formosanus, infection of molluscs occurs passively by ingestion of eggs, followed by a long intramolluscan phase. We compare these data with those described for other Heterophyidae, and discuss on the phylogenetic background of the pattern of miracidial development verified in these parasites. |
publishDate |
2018 |
dc.date.issued.fl_str_mv |
2018 |
dc.date.accessioned.fl_str_mv |
2023-07-14T20:27:02Z |
dc.date.available.fl_str_mv |
2023-07-14T20:27:02Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1843/56290 |
dc.identifier.doi.pt_BR.fl_str_mv |
https://doi.org/10.1371/journal.pone.0194161 |
dc.identifier.issn.pt_BR.fl_str_mv |
1932-6203 |
dc.identifier.orcid.pt_BR.fl_str_mv |
https://orcid.org/0000-0002-5519-1348 https://orcid.org/0000-0001-8799-4885 https://orcid.org/0000-0002-9039-3068 https://orcid.org/0000-0001-7118-7210 |
url |
https://doi.org/10.1371/journal.pone.0194161 http://hdl.handle.net/1843/56290 https://orcid.org/0000-0002-5519-1348 https://orcid.org/0000-0001-8799-4885 https://orcid.org/0000-0002-9039-3068 https://orcid.org/0000-0001-7118-7210 |
identifier_str_mv |
1932-6203 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
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PLoS One |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Minas Gerais |
dc.publisher.initials.fl_str_mv |
UFMG |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
ICB - DEPARTAMENTO DE PARASITOLOGIA |
publisher.none.fl_str_mv |
Universidade Federal de Minas Gerais |
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reponame:Repositório Institucional da UFMG instname:Universidade Federal de Minas Gerais (UFMG) instacron:UFMG |
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UFMG |
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