The apical root canal system microbial communities determined by next-generation sequencing

Detalhes bibliográficos
Autor(a) principal: Luciana Carla Nevesde Brito
Data de Publicação: 2020
Outros Autores: Janet Doolittle-Hall, Chun-Teh Lee, Kevin Moss, Wilson Bambirra Júnior, Warley Luciano Fonseca Tavares, Antônio Paulino Ribeiro Sobrinho, Flávia Rocha Fonseca Teles
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFMG
Texto Completo: https://doi.org/10.1038/s41598-020-67828-3
http://hdl.handle.net/1843/46489
Resumo: CNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
id UFMG_12c94a95cb774f46416ab1210a68afd5
oai_identifier_str oai:repositorio.ufmg.br:1843/46489
network_acronym_str UFMG
network_name_str Repositório Institucional da UFMG
repository_id_str
spelling 2022-10-21T14:47:29Z2022-10-21T14:47:29Z2020-07-021010108https://doi.org/10.1038/s41598-020-67828-320452322http://hdl.handle.net/1843/46489CNPq - Conselho Nacional de Desenvolvimento Científico e TecnológicoCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível SuperiorOutra AgênciaThe aim of this study was to explore the microbial communities of endodontic infections at their apical portion by 16S rRNA Illumina sequencing and delineate the core microbiome of root canal infections and that of their associated clinical symptomatology. Samples were collected from fifteen subjects presenting one tooth with a root canal infection, and their associated symptoms were recorded. Samples were collected from the apical third of roots using a #10 K file and then amplified using multiple displacement amplification and PCR-amplified with universal primers. Amplicons were sequenced (V3-V4 hypervariable region of the 16S rRNA gene) using MiSeq (Illumina, CA). The microbial composition of the samples was determined using QIIME and HOMINGS. Data were analyzed using t tests and ANOVA. A total of 1,038,656 good quality sequences were obtained, and OTUs were assigned to 10 bacterial phyla, led by Bacteroidetes (51.2%) and Firmicutes (27.1%), and 94 genera were represented primarily by Prevotella (17.9%) and Bacteroidaceae G-1 (14.3%). Symptomatic teeth were associated with higher levels of Porphyromonas (p < 0.05) and Prevotella. P. endodontalis and P. oris were present in both cores. The present study demonstrated the complexity of the root canal microbiome and the "common denominators" of root canal infections and identified taxa whose virulence properties should be further explored. The polymicrobial etiology of endodontic infections has long been established. However, few studies have focused on expanding the breadth and depth of coverage of microbiome-infected root canals at their apical portionengUniversidade Federal de Minas GeraisUFMGBrasilFAO - DEPARTAMENTO DE ODONTOLOGIA RESTAURADORAScientific ReportsInfectionsDental pulp cavityEndodonticsRNA ribosomal 16sHost microbial interactionsThe apical root canal system microbial communities determined by next-generation sequencinginfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7331743/Luciana Carla Nevesde BritoJanet Doolittle-HallChun-Teh LeeKevin MossWilson Bambirra JúniorWarley Luciano Fonseca TavaresAntônio Paulino Ribeiro SobrinhoFlávia Rocha Fonseca Telesapplication/pdfinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMGinstname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMGLICENSELicense.txtLicense.txttext/plain; charset=utf-82042https://repositorio.ufmg.br/bitstream/1843/46489/1/License.txtfa505098d172de0bc8864fc1287ffe22MD51ORIGINALThe apical root canal system microbial communities determined by next generation sequencing.pdfThe apical root canal system microbial communities determined by next generation sequencing.pdfapplication/pdf2247686https://repositorio.ufmg.br/bitstream/1843/46489/2/The%20apical%20root%20canal%20system%20microbial%20communities%20determined%20by%20next%20generation%20sequencing.pdf240ed18175bfbb8caefb54a4b8e80b74MD521843/464892022-10-21 11:47:30.371oai:repositorio.ufmg.br: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Repositório de PublicaçõesPUBhttps://repositorio.ufmg.br/oaiopendoar:2022-10-21T14:47:30Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)false
dc.title.pt_BR.fl_str_mv The apical root canal system microbial communities determined by next-generation sequencing
title The apical root canal system microbial communities determined by next-generation sequencing
spellingShingle The apical root canal system microbial communities determined by next-generation sequencing
Luciana Carla Nevesde Brito
Infections
Dental pulp cavity
Endodontics
RNA ribosomal 16s
Host microbial interactions
title_short The apical root canal system microbial communities determined by next-generation sequencing
title_full The apical root canal system microbial communities determined by next-generation sequencing
title_fullStr The apical root canal system microbial communities determined by next-generation sequencing
title_full_unstemmed The apical root canal system microbial communities determined by next-generation sequencing
title_sort The apical root canal system microbial communities determined by next-generation sequencing
author Luciana Carla Nevesde Brito
author_facet Luciana Carla Nevesde Brito
Janet Doolittle-Hall
Chun-Teh Lee
Kevin Moss
Wilson Bambirra Júnior
Warley Luciano Fonseca Tavares
Antônio Paulino Ribeiro Sobrinho
Flávia Rocha Fonseca Teles
author_role author
author2 Janet Doolittle-Hall
Chun-Teh Lee
Kevin Moss
Wilson Bambirra Júnior
Warley Luciano Fonseca Tavares
Antônio Paulino Ribeiro Sobrinho
Flávia Rocha Fonseca Teles
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Luciana Carla Nevesde Brito
Janet Doolittle-Hall
Chun-Teh Lee
Kevin Moss
Wilson Bambirra Júnior
Warley Luciano Fonseca Tavares
Antônio Paulino Ribeiro Sobrinho
Flávia Rocha Fonseca Teles
dc.subject.other.pt_BR.fl_str_mv Infections
Dental pulp cavity
Endodontics
RNA ribosomal 16s
Host microbial interactions
topic Infections
Dental pulp cavity
Endodontics
RNA ribosomal 16s
Host microbial interactions
description CNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
publishDate 2020
dc.date.issued.fl_str_mv 2020-07-02
dc.date.accessioned.fl_str_mv 2022-10-21T14:47:29Z
dc.date.available.fl_str_mv 2022-10-21T14:47:29Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1843/46489
dc.identifier.doi.pt_BR.fl_str_mv https://doi.org/10.1038/s41598-020-67828-3
dc.identifier.issn.pt_BR.fl_str_mv 20452322
url https://doi.org/10.1038/s41598-020-67828-3
http://hdl.handle.net/1843/46489
identifier_str_mv 20452322
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartof.pt_BR.fl_str_mv Scientific Reports
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Minas Gerais
dc.publisher.initials.fl_str_mv UFMG
dc.publisher.country.fl_str_mv Brasil
dc.publisher.department.fl_str_mv FAO - DEPARTAMENTO DE ODONTOLOGIA RESTAURADORA
publisher.none.fl_str_mv Universidade Federal de Minas Gerais
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFMG
instname:Universidade Federal de Minas Gerais (UFMG)
instacron:UFMG
instname_str Universidade Federal de Minas Gerais (UFMG)
instacron_str UFMG
institution UFMG
reponame_str Repositório Institucional da UFMG
collection Repositório Institucional da UFMG
bitstream.url.fl_str_mv https://repositorio.ufmg.br/bitstream/1843/46489/1/License.txt
https://repositorio.ufmg.br/bitstream/1843/46489/2/The%20apical%20root%20canal%20system%20microbial%20communities%20determined%20by%20next%20generation%20sequencing.pdf
bitstream.checksum.fl_str_mv fa505098d172de0bc8864fc1287ffe22
240ed18175bfbb8caefb54a4b8e80b74
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
repository.name.fl_str_mv Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)
repository.mail.fl_str_mv
_version_ 1803589576178008064

Registros relacionados