Phenylketonuria and Gut Microbiota: A Controlled Study Based on Next-Generation Sequencing.

Detalhes bibliográficos
Autor(a) principal: Oliveira, Felipe Pinheiro de
Data de Publicação: 2016
Outros Autores: Mendes, Roberta Hack, Dobbler, Priscila Thiago, Mai, Volker, Pylro, Victor Satler, Waugh, Sheldon G, Vairo, Filippo, Refosco, Lilia Farret, Roesch, Luiz Fernando Würdig, Schwartz, Ida Vanessa Doederlein
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
DOI: 10.1371/journal.pone.015751
Texto Completo: https://www.arca.fiocruz.br/handle/icict/18434
Resumo: Universidade Federal do Rio Grande do Sul. Programa de Pós-Graduação em Genética e Biologia Molecular. Porto Alegre, RS, Brazil/Hospital de Clínicas de Porto Alegre. Centro de Pesquisa Experimental. Laboratório Basic Research and Advanced Investigations in Neurosciences. Porto Alegre, RS, Brazil
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spelling Oliveira, Felipe Pinheiro deMendes, Roberta HackDobbler, Priscila ThiagoMai, VolkerPylro, Victor SatlerWaugh, Sheldon GVairo, FilippoRefosco, Lilia FarretRoesch, Luiz Fernando WürdigSchwartz, Ida Vanessa Doederlein2017-04-12T19:10:17Z2017-04-12T19:10:17Z2016OLIVEIRA, Felipe Pinheiro de et al. Phenylketonuria and Gut Microbiota: A Controlled Study Based on Next-Generation Sequencing. PLoS ONE, v. 11, n. 6, e0157513, 20161932-6203https://www.arca.fiocruz.br/handle/icict/1843410.1371/journal.pone.015751engPublic Library of SciencefenilcetonuriaPhenylketonuriaMicrobiomeGut bactériaDietMicrobial ecologyClostridiumRibosomal RNAProtein metabolismPhenylketonuria and Gut Microbiota: A Controlled Study Based on Next-Generation Sequencing.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Rio Grande do Sul. Programa de Pós-Graduação em Genética e Biologia Molecular. Porto Alegre, RS, Brazil/Hospital de Clínicas de Porto Alegre. Centro de Pesquisa Experimental. Laboratório Basic Research and Advanced Investigations in Neurosciences. Porto Alegre, RS, BrazilUniversidade Federal do Rio Grande do Sul. Programa de Pós-Graduação em Genética e Biologia Molecular. Porto Alegre, RS, Brazil/Hospital de Clínicas de Porto Alegre. Centro de Pesquisa Experimental. Laboratório Basic Research and Advanced Investigations in Neurosciences. Porto Alegre, RS, BrazilUniversidade Federal do Pampa. Centro Interdisciplinar de Pesquisas em Biotecnologia–CIP-Biotec. São Gabriel, RS, BrazilUniversity of Florida. Emerging Pathogens Institute. College of Public Health and Health Professions and College of Medicine. Department of Epidemiology. Gainesville, FL, United States of AmericaOswaldo Cruz Fundation. René Rachou Research Centre. Genomics and Computational Biology Group. Belo Horizonte, MG, BrazilUniversity of Florida. Emerging Pathogens Institute. College of Public Health and Health Professions and College of Medicine. Department of Epidemiology. Gainesville, FL, United States of AmericaHospital de Clínicas de Porto Alegre. Serviço de Genética Médica. Porto Alegre, RS, BrazilHospital de Clínicas de Porto Alegre. Serviço de Genética Médica. Porto Alegre, RS, BrazilUniversidade Federal do Pampa. Centro Interdisciplinar de Pesquisas em Biotecnologia–CIP-Biotec. São Gabriel, RS, BrazilUniversidade Federal do Rio Grande do Sul. Programa de Pós-Graduação em Genética e Biologia Molecular. Porto Alegre, RS, Brazil/Hospital de Clínicas de Porto Alegre. Centro de Pesquisa Experimental. Laboratório de Pesquisas Básicas e Aplicadas em Neurociencias. Porto Alegre, RS, BrazilPhenylketonuria (PKU) is an inborn error of metabolism associated with high blood levels of phenylalanine (Phe). A Phe-restricted diet supplemented with L-amino acids is the main treatment strategy for this disease; if started early, most neurological abnormalities can be prevented. The healthy human gut contains trillions of commensal bacteria, often referred to as the gut microbiota. The composition of the gut microbiota is known to be modulated by environmental factors, including diet. In this study, we compared the gut microbiota of 8 PKU patients on Phe-restricted dietary treatment with that of 10 healthy individuals. The microbiota were characterized by 16S rRNA sequencing using the Ion Torrent™ platform. The most dominant phyla detected in both groups were Bacteroidetes and Firmicutes. PKU patients showed reduced abundance of the Clostridiaceae, Erysipelotrichaceae, and Lachnospiraceae families, Clostridiales class, Coprococcus, Dorea, Lachnospira, Odoribacter, Ruminococcus and Veillonella genera, and enrichment of Prevotella, Akkermansia, and Peptostreptococcaceae. Microbial function prediction suggested significant differences in starch/glucose and amino acid metabolism between PKU patients and controls. Together, our results suggest the presence of distinct taxonomic groups within the gut microbiome of PKU patients, which may be modulated by their plasma Phe concentration. Whether our findings represent an effect of the disease itself, or a consequence of the modified diet is unclear.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83082https://www.arca.fiocruz.br/bitstream/icict/18434/1/license.txt9193a7c197bc67acd023525e72a03240MD51ORIGINALve_Oliveira_Felipe_Phenylketonuria_CPqRR_2016.pdfve_Oliveira_Felipe_Phenylketonuria_CPqRR_2016.pdfapplication/pdf5478745https://www.arca.fiocruz.br/bitstream/icict/18434/2/ve_Oliveira_Felipe_Phenylketonuria_CPqRR_2016.pdf1d0d9580be46c56f2b8a9c16a83097e5MD52TEXTve_Oliveira_Felipe_Phenylketonuria_CPqRR_2016.pdf.txtve_Oliveira_Felipe_Phenylketonuria_CPqRR_2016.pdf.txtExtracted texttext/plain294https://www.arca.fiocruz.br/bitstream/icict/18434/3/ve_Oliveira_Felipe_Phenylketonuria_CPqRR_2016.pdf.txt243499113af297c8a970517779733f61MD53icict/184342022-02-09 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dc.title.pt_BR.fl_str_mv Phenylketonuria and Gut Microbiota: A Controlled Study Based on Next-Generation Sequencing.
title Phenylketonuria and Gut Microbiota: A Controlled Study Based on Next-Generation Sequencing.
spellingShingle Phenylketonuria and Gut Microbiota: A Controlled Study Based on Next-Generation Sequencing.
Oliveira, Felipe Pinheiro de
fenilcetonuria
Phenylketonuria
Microbiome
Gut bactéria
Diet
Microbial ecology
Clostridium
Ribosomal RNA
Protein metabolism
title_short Phenylketonuria and Gut Microbiota: A Controlled Study Based on Next-Generation Sequencing.
title_full Phenylketonuria and Gut Microbiota: A Controlled Study Based on Next-Generation Sequencing.
title_fullStr Phenylketonuria and Gut Microbiota: A Controlled Study Based on Next-Generation Sequencing.
title_full_unstemmed Phenylketonuria and Gut Microbiota: A Controlled Study Based on Next-Generation Sequencing.
title_sort Phenylketonuria and Gut Microbiota: A Controlled Study Based on Next-Generation Sequencing.
author Oliveira, Felipe Pinheiro de
author_facet Oliveira, Felipe Pinheiro de
Mendes, Roberta Hack
Dobbler, Priscila Thiago
Mai, Volker
Pylro, Victor Satler
Waugh, Sheldon G
Vairo, Filippo
Refosco, Lilia Farret
Roesch, Luiz Fernando Würdig
Schwartz, Ida Vanessa Doederlein
author_role author
author2 Mendes, Roberta Hack
Dobbler, Priscila Thiago
Mai, Volker
Pylro, Victor Satler
Waugh, Sheldon G
Vairo, Filippo
Refosco, Lilia Farret
Roesch, Luiz Fernando Würdig
Schwartz, Ida Vanessa Doederlein
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Oliveira, Felipe Pinheiro de
Mendes, Roberta Hack
Dobbler, Priscila Thiago
Mai, Volker
Pylro, Victor Satler
Waugh, Sheldon G
Vairo, Filippo
Refosco, Lilia Farret
Roesch, Luiz Fernando Würdig
Schwartz, Ida Vanessa Doederlein
dc.subject.other.pt_BR.fl_str_mv fenilcetonuria
topic fenilcetonuria
Phenylketonuria
Microbiome
Gut bactéria
Diet
Microbial ecology
Clostridium
Ribosomal RNA
Protein metabolism
dc.subject.en.pt_BR.fl_str_mv Phenylketonuria
Microbiome
Gut bactéria
Diet
Microbial ecology
Clostridium
Ribosomal RNA
Protein metabolism
description Universidade Federal do Rio Grande do Sul. Programa de Pós-Graduação em Genética e Biologia Molecular. Porto Alegre, RS, Brazil/Hospital de Clínicas de Porto Alegre. Centro de Pesquisa Experimental. Laboratório Basic Research and Advanced Investigations in Neurosciences. Porto Alegre, RS, Brazil
publishDate 2016
dc.date.issued.fl_str_mv 2016
dc.date.accessioned.fl_str_mv 2017-04-12T19:10:17Z
dc.date.available.fl_str_mv 2017-04-12T19:10:17Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv OLIVEIRA, Felipe Pinheiro de et al. Phenylketonuria and Gut Microbiota: A Controlled Study Based on Next-Generation Sequencing. PLoS ONE, v. 11, n. 6, e0157513, 2016
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/18434
dc.identifier.issn.pt_BR.fl_str_mv 1932-6203
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pone.015751
identifier_str_mv OLIVEIRA, Felipe Pinheiro de et al. Phenylketonuria and Gut Microbiota: A Controlled Study Based on Next-Generation Sequencing. PLoS ONE, v. 11, n. 6, e0157513, 2016
1932-6203
10.1371/journal.pone.015751
url https://www.arca.fiocruz.br/handle/icict/18434
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
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