Phenylketonuria and Gut Microbiota: A Controlled Study Based on Next-Generation Sequencing.
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
DOI: | 10.1371/journal.pone.015751 |
Texto Completo: | https://www.arca.fiocruz.br/handle/icict/18434 |
Resumo: | Universidade Federal do Rio Grande do Sul. Programa de Pós-Graduação em Genética e Biologia Molecular. Porto Alegre, RS, Brazil/Hospital de Clínicas de Porto Alegre. Centro de Pesquisa Experimental. Laboratório Basic Research and Advanced Investigations in Neurosciences. Porto Alegre, RS, Brazil |
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Oliveira, Felipe Pinheiro deMendes, Roberta HackDobbler, Priscila ThiagoMai, VolkerPylro, Victor SatlerWaugh, Sheldon GVairo, FilippoRefosco, Lilia FarretRoesch, Luiz Fernando WürdigSchwartz, Ida Vanessa Doederlein2017-04-12T19:10:17Z2017-04-12T19:10:17Z2016OLIVEIRA, Felipe Pinheiro de et al. Phenylketonuria and Gut Microbiota: A Controlled Study Based on Next-Generation Sequencing. PLoS ONE, v. 11, n. 6, e0157513, 20161932-6203https://www.arca.fiocruz.br/handle/icict/1843410.1371/journal.pone.015751engPublic Library of SciencefenilcetonuriaPhenylketonuriaMicrobiomeGut bactériaDietMicrobial ecologyClostridiumRibosomal RNAProtein metabolismPhenylketonuria and Gut Microbiota: A Controlled Study Based on Next-Generation Sequencing.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Rio Grande do Sul. Programa de Pós-Graduação em Genética e Biologia Molecular. Porto Alegre, RS, Brazil/Hospital de Clínicas de Porto Alegre. Centro de Pesquisa Experimental. Laboratório Basic Research and Advanced Investigations in Neurosciences. Porto Alegre, RS, BrazilUniversidade Federal do Rio Grande do Sul. Programa de Pós-Graduação em Genética e Biologia Molecular. Porto Alegre, RS, Brazil/Hospital de Clínicas de Porto Alegre. Centro de Pesquisa Experimental. Laboratório Basic Research and Advanced Investigations in Neurosciences. Porto Alegre, RS, BrazilUniversidade Federal do Pampa. Centro Interdisciplinar de Pesquisas em Biotecnologia–CIP-Biotec. São Gabriel, RS, BrazilUniversity of Florida. Emerging Pathogens Institute. College of Public Health and Health Professions and College of Medicine. Department of Epidemiology. Gainesville, FL, United States of AmericaOswaldo Cruz Fundation. René Rachou Research Centre. Genomics and Computational Biology Group. Belo Horizonte, MG, BrazilUniversity of Florida. Emerging Pathogens Institute. College of Public Health and Health Professions and College of Medicine. Department of Epidemiology. Gainesville, FL, United States of AmericaHospital de Clínicas de Porto Alegre. Serviço de Genética Médica. Porto Alegre, RS, BrazilHospital de Clínicas de Porto Alegre. Serviço de Genética Médica. Porto Alegre, RS, BrazilUniversidade Federal do Pampa. Centro Interdisciplinar de Pesquisas em Biotecnologia–CIP-Biotec. São Gabriel, RS, BrazilUniversidade Federal do Rio Grande do Sul. Programa de Pós-Graduação em Genética e Biologia Molecular. Porto Alegre, RS, Brazil/Hospital de Clínicas de Porto Alegre. Centro de Pesquisa Experimental. Laboratório de Pesquisas Básicas e Aplicadas em Neurociencias. Porto Alegre, RS, BrazilPhenylketonuria (PKU) is an inborn error of metabolism associated with high blood levels of phenylalanine (Phe). A Phe-restricted diet supplemented with L-amino acids is the main treatment strategy for this disease; if started early, most neurological abnormalities can be prevented. The healthy human gut contains trillions of commensal bacteria, often referred to as the gut microbiota. The composition of the gut microbiota is known to be modulated by environmental factors, including diet. In this study, we compared the gut microbiota of 8 PKU patients on Phe-restricted dietary treatment with that of 10 healthy individuals. The microbiota were characterized by 16S rRNA sequencing using the Ion Torrent™ platform. The most dominant phyla detected in both groups were Bacteroidetes and Firmicutes. PKU patients showed reduced abundance of the Clostridiaceae, Erysipelotrichaceae, and Lachnospiraceae families, Clostridiales class, Coprococcus, Dorea, Lachnospira, Odoribacter, Ruminococcus and Veillonella genera, and enrichment of Prevotella, Akkermansia, and Peptostreptococcaceae. Microbial function prediction suggested significant differences in starch/glucose and amino acid metabolism between PKU patients and controls. Together, our results suggest the presence of distinct taxonomic groups within the gut microbiome of PKU patients, which may be modulated by their plasma Phe concentration. Whether our findings represent an effect of the disease itself, or a consequence of the modified diet is unclear.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83082https://www.arca.fiocruz.br/bitstream/icict/18434/1/license.txt9193a7c197bc67acd023525e72a03240MD51ORIGINALve_Oliveira_Felipe_Phenylketonuria_CPqRR_2016.pdfve_Oliveira_Felipe_Phenylketonuria_CPqRR_2016.pdfapplication/pdf5478745https://www.arca.fiocruz.br/bitstream/icict/18434/2/ve_Oliveira_Felipe_Phenylketonuria_CPqRR_2016.pdf1d0d9580be46c56f2b8a9c16a83097e5MD52TEXTve_Oliveira_Felipe_Phenylketonuria_CPqRR_2016.pdf.txtve_Oliveira_Felipe_Phenylketonuria_CPqRR_2016.pdf.txtExtracted texttext/plain294https://www.arca.fiocruz.br/bitstream/icict/18434/3/ve_Oliveira_Felipe_Phenylketonuria_CPqRR_2016.pdf.txt243499113af297c8a970517779733f61MD53icict/184342022-02-09 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dc.title.pt_BR.fl_str_mv |
Phenylketonuria and Gut Microbiota: A Controlled Study Based on Next-Generation Sequencing. |
title |
Phenylketonuria and Gut Microbiota: A Controlled Study Based on Next-Generation Sequencing. |
spellingShingle |
Phenylketonuria and Gut Microbiota: A Controlled Study Based on Next-Generation Sequencing. Oliveira, Felipe Pinheiro de fenilcetonuria Phenylketonuria Microbiome Gut bactéria Diet Microbial ecology Clostridium Ribosomal RNA Protein metabolism |
title_short |
Phenylketonuria and Gut Microbiota: A Controlled Study Based on Next-Generation Sequencing. |
title_full |
Phenylketonuria and Gut Microbiota: A Controlled Study Based on Next-Generation Sequencing. |
title_fullStr |
Phenylketonuria and Gut Microbiota: A Controlled Study Based on Next-Generation Sequencing. |
title_full_unstemmed |
Phenylketonuria and Gut Microbiota: A Controlled Study Based on Next-Generation Sequencing. |
title_sort |
Phenylketonuria and Gut Microbiota: A Controlled Study Based on Next-Generation Sequencing. |
author |
Oliveira, Felipe Pinheiro de |
author_facet |
Oliveira, Felipe Pinheiro de Mendes, Roberta Hack Dobbler, Priscila Thiago Mai, Volker Pylro, Victor Satler Waugh, Sheldon G Vairo, Filippo Refosco, Lilia Farret Roesch, Luiz Fernando Würdig Schwartz, Ida Vanessa Doederlein |
author_role |
author |
author2 |
Mendes, Roberta Hack Dobbler, Priscila Thiago Mai, Volker Pylro, Victor Satler Waugh, Sheldon G Vairo, Filippo Refosco, Lilia Farret Roesch, Luiz Fernando Würdig Schwartz, Ida Vanessa Doederlein |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Oliveira, Felipe Pinheiro de Mendes, Roberta Hack Dobbler, Priscila Thiago Mai, Volker Pylro, Victor Satler Waugh, Sheldon G Vairo, Filippo Refosco, Lilia Farret Roesch, Luiz Fernando Würdig Schwartz, Ida Vanessa Doederlein |
dc.subject.other.pt_BR.fl_str_mv |
fenilcetonuria |
topic |
fenilcetonuria Phenylketonuria Microbiome Gut bactéria Diet Microbial ecology Clostridium Ribosomal RNA Protein metabolism |
dc.subject.en.pt_BR.fl_str_mv |
Phenylketonuria Microbiome Gut bactéria Diet Microbial ecology Clostridium Ribosomal RNA Protein metabolism |
description |
Universidade Federal do Rio Grande do Sul. Programa de Pós-Graduação em Genética e Biologia Molecular. Porto Alegre, RS, Brazil/Hospital de Clínicas de Porto Alegre. Centro de Pesquisa Experimental. Laboratório Basic Research and Advanced Investigations in Neurosciences. Porto Alegre, RS, Brazil |
publishDate |
2016 |
dc.date.issued.fl_str_mv |
2016 |
dc.date.accessioned.fl_str_mv |
2017-04-12T19:10:17Z |
dc.date.available.fl_str_mv |
2017-04-12T19:10:17Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
OLIVEIRA, Felipe Pinheiro de et al. Phenylketonuria and Gut Microbiota: A Controlled Study Based on Next-Generation Sequencing. PLoS ONE, v. 11, n. 6, e0157513, 2016 |
dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/18434 |
dc.identifier.issn.pt_BR.fl_str_mv |
1932-6203 |
dc.identifier.doi.none.fl_str_mv |
10.1371/journal.pone.015751 |
identifier_str_mv |
OLIVEIRA, Felipe Pinheiro de et al. Phenylketonuria and Gut Microbiota: A Controlled Study Based on Next-Generation Sequencing. PLoS ONE, v. 11, n. 6, e0157513, 2016 1932-6203 10.1371/journal.pone.015751 |
url |
https://www.arca.fiocruz.br/handle/icict/18434 |
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eng |
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eng |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Public Library of Science |
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Public Library of Science |
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