Evaluation of different sites for blood collection for β-hydroxybutyrate assessment in dairy cows using hand-held meter

• Autor(a) principal: Emerson A.alvarenga
• Data de Publicação: 2019
• Outros Autores: Gustavo Henrique Ferreira Abreu Moreira, Elias Facury Filho, Fabiola de Oliveira Paes Leme, Tiago Facury Moreira, Antônio Último de Carvalho
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UFMG
• Texto Completo: https://doi.org/10.1590/1678-4162-10164; http://hdl.handle.net/1843/67347; http://orcid.org/0000-0002-4470-6246; http://orcid.org/0000-0001-8456-3297; http://orcid.org/0000-0003-0836-2105; http://orcid.org/0000-0002-4013-0147; http://orcid.org/0000-0002-0808-1955; http://orcid.org/0000-0002-8041-796X
• topic: Dairy cows; Ketosis; Transition period; Diagnosis; Vaca leiteira; Cetose; Coleta de amostras sanguíneas; Diagnóstico

Subclinical ketosis is a major concern in dairy herds, and its diagnosis and prevention can have a major impact on animal health. This study compared four different sites for collecting blood samples (jugular, mammary and coccygeal veins, and tip of the tail) for measuring β-hydroxybutyrate (BHBA), using an automatic portable meter. Six Holstein cows were used, and blood collection was carried out on the second, fifth, 10th, 15th and 21st days postpartum. The portable meter results were similar to the laboratory results and showed a strong correlation of 0.83. Serum BHBA concentrations in blood samples collected from the tip of the tail, jugular and coccygeal were similar. However, blood taken from the mammary vein had a lower concentration. Therefore, blood samples for measuring BHBA can be collected from the jugular and coccygeal veins and the tip of the tail, the latter two being the easiest options for monitoring BHBA in a dairy herd.