Anthelmintic activity of Annona crassiflora leaves against Haemonchus contortus: part 1: in vitroinhibition of the hatchability and larval development
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFMG |
Texto Completo: | https://doi.org/10.26605/medvet-v13n2-3067 http://hdl.handle.net/1843/41599 |
Resumo: | We evaluated the activity of Annona crassiflora leaves against Haemonchus contortus resistant to albendazol. Aqueous (AE), ethanolic (EE) and ethyl acetate (EAE) extracts were produced and the predominant presence of flavonoids was observed in HPLC-DAD chromatograms. Initially we evaluated the larval development inhibition (LDI) of dry A. crassiflora leaf powder or its AE directly in fecal quantitative cultures. The efficacies of the extracts, with or without tannins, on egg hatching inhibition (EHI) were investigated reveling that the EE was the most effective (LC90 = 8.96 mg/mL). However, after tannin removal, AE showed the highest activity (LC90 = 4.27 mg/mL). In the LDI test, the LC90 of AE was < 6.25 mg/g of fecal culture and to leaf powder of leaves was 69.14 mg/g. High efficacies of AE and EE for EHI were detected and the tannins were not the main active metabolites. The anthelmintic potential of this plant could be attributed to association between flavonoids and other metabolites. |
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2022-05-12T11:59:30Z2022-05-12T11:59:30Z2019-07132184191https://doi.org/10.26605/medvet-v13n2-30671809-4678http://hdl.handle.net/1843/41599We evaluated the activity of Annona crassiflora leaves against Haemonchus contortus resistant to albendazol. Aqueous (AE), ethanolic (EE) and ethyl acetate (EAE) extracts were produced and the predominant presence of flavonoids was observed in HPLC-DAD chromatograms. Initially we evaluated the larval development inhibition (LDI) of dry A. crassiflora leaf powder or its AE directly in fecal quantitative cultures. The efficacies of the extracts, with or without tannins, on egg hatching inhibition (EHI) were investigated reveling that the EE was the most effective (LC90 = 8.96 mg/mL). However, after tannin removal, AE showed the highest activity (LC90 = 4.27 mg/mL). In the LDI test, the LC90 of AE was < 6.25 mg/g of fecal culture and to leaf powder of leaves was 69.14 mg/g. High efficacies of AE and EE for EHI were detected and the tannins were not the main active metabolites. The anthelmintic potential of this plant could be attributed to association between flavonoids and other metabolites.Avaliou-se a atividade de folhas de Annona crassifloracontra Haemonchus contortusresistentes ao albendazol. Extratos aquosos (EA), etanólicos (EE) e acetato de etila (EAE) foram produzidos e a presença predominante de flavonoides foi observada em cromatogramas HPLC-DAD. Inicialmente avaliou-se a inibição do desenvolvimento larval (IDL) do pó seco de folhas de A. crassiflorae seu EA diretamente em coproculturas quantitativas. As eficácias dos extratos, com ou sem taninos, na inibição da eclosão dos ovos (IEO) foram investigadas revelando que o EE foi o mais efetivo (CL90= 8,96 mg/mL). No entanto, após a remoção do tanino, o EA apresentou a maior atividade (CL90= 4,27 mg/mL). No teste IDL, a CL90de EA foi <6,25 mg/g na coprocultura e o pó desidratado das folhas foi de 69,14 mg/g. A alta eficácia do EA eEE para IEO foram detectados e os taninos não foram os principais metabólitos ativos. O potencial anti-helmíntico dessa planta poderia ser atribuído à associação entre flavonoides e outros metabólitos.CNPq - Conselho Nacional de Desenvolvimento Científico e TecnológicoFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas GeraisCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível SuperiorOutra AgênciaporUniversidade Federal de Minas GeraisUFMGBrasilICA - INSTITUTO DE CIÊNCIAS AGRÁRIASMedicina VeterináriaHaemonchus contortusPlantas - NematódeosFlavonoidesAnthelmintic activity of Annona crassiflora leaves against Haemonchus contortus: part 1: in vitroinhibition of the hatchability and larval developmentAtividade antihelmíntica de folhas de Annona crassiflora contra Haemonchus contortus: parte 1: inibição in vitro da eclodibilidade e do desenvolvimento larvalinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://www.journals.ufrpe.br/index.php/medicinaveterinaria/article/view/3067Marco Antônio Martins CaldeiraFranciellen Morais CostaKaike Magno de MacêdoValdo Soares Martins JúniorDheison Emanuel Augusto PereiraAna Claúdia Maia SoaresIsabela Parolis MartinsIdael Matheus Góes LopesFernão Castro BragaNeide Judith Faria de OliveiraEduardo Robson Duarteinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMGinstname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMGLICENSELicense.txtLicense.txttext/plain; charset=utf-82042https://repositorio.ufmg.br/bitstream/1843/41599/1/License.txtfa505098d172de0bc8864fc1287ffe22MD51ORIGINALAnthelmintic activity of annona crassiflora leaves against haemonchus contortus part 1 in vitro inhibition of the hatchability and larval development.pdfAnthelmintic activity of annona crassiflora leaves against haemonchus contortus part 1 in vitro inhibition of the hatchability and larval development.pdfapplication/pdf322604https://repositorio.ufmg.br/bitstream/1843/41599/2/Anthelmintic%20activity%20of%20annona%20crassiflora%20leaves%20against%20haemonchus%20contortus%20part%201%20in%20vitro%20inhibition%20of%20the%20hatchability%20and%20larval%20development.pdfdcd084166e2cfdaecdf12f660240de0bMD521843/415992022-05-12 08:59:30.933oai:repositorio.ufmg.br:1843/41599TElDRU7vv71BIERFIERJU1RSSUJVSe+/ve+/vU8gTu+/vU8tRVhDTFVTSVZBIERPIFJFUE9TSVTvv71SSU8gSU5TVElUVUNJT05BTCBEQSBVRk1HCiAKCkNvbSBhIGFwcmVzZW50Ye+/ve+/vW8gZGVzdGEgbGljZW7vv71hLCB2b2Pvv70gKG8gYXV0b3IgKGVzKSBvdSBvIHRpdHVsYXIgZG9zIGRpcmVpdG9zIGRlIGF1dG9yKSBjb25jZWRlIGFvIFJlcG9zaXTvv71yaW8gSW5zdGl0dWNpb25hbCBkYSBVRk1HIChSSS1VRk1HKSBvIGRpcmVpdG8gbu+/vW8gZXhjbHVzaXZvIGUgaXJyZXZvZ++/vXZlbCBkZSByZXByb2R1emlyIGUvb3UgZGlzdHJpYnVpciBhIHN1YSBwdWJsaWNh77+977+9byAoaW5jbHVpbmRvIG8gcmVzdW1vKSBwb3IgdG9kbyBvIG11bmRvIG5vIGZvcm1hdG8gaW1wcmVzc28gZSBlbGV0cu+/vW5pY28gZSBlbSBxdWFscXVlciBtZWlvLCBpbmNsdWluZG8gb3MgZm9ybWF0b3Mg77+9dWRpbyBvdSB277+9ZGVvLgoKVm9j77+9IGRlY2xhcmEgcXVlIGNvbmhlY2UgYSBwb2zvv710aWNhIGRlIGNvcHlyaWdodCBkYSBlZGl0b3JhIGRvIHNldSBkb2N1bWVudG8gZSBxdWUgY29uaGVjZSBlIGFjZWl0YSBhcyBEaXJldHJpemVzIGRvIFJJLVVGTUcuCgpWb2Pvv70gY29uY29yZGEgcXVlIG8gUmVwb3NpdO+/vXJpbyBJbnN0aXR1Y2lvbmFsIGRhIFVGTUcgcG9kZSwgc2VtIGFsdGVyYXIgbyBjb250Ze+/vWRvLCB0cmFuc3BvciBhIHN1YSBwdWJsaWNh77+977+9byBwYXJhIHF1YWxxdWVyIG1laW8gb3UgZm9ybWF0byBwYXJhIGZpbnMgZGUgcHJlc2VydmHvv73vv71vLgoKVm9j77+9IHRhbWLvv71tIGNvbmNvcmRhIHF1ZSBvIFJlcG9zaXTvv71yaW8gSW5zdGl0dWNpb25hbCBkYSBVRk1HIHBvZGUgbWFudGVyIG1haXMgZGUgdW1hIGPvv71waWEgZGUgc3VhIHB1YmxpY2Hvv73vv71vIHBhcmEgZmlucyBkZSBzZWd1cmFu77+9YSwgYmFjay11cCBlIHByZXNlcnZh77+977+9by4KClZvY++/vSBkZWNsYXJhIHF1ZSBhIHN1YSBwdWJsaWNh77+977+9byDvv70gb3JpZ2luYWwgZSBxdWUgdm9j77+9IHRlbSBvIHBvZGVyIGRlIGNvbmNlZGVyIG9zIGRpcmVpdG9zIGNvbnRpZG9zIG5lc3RhIGxpY2Vu77+9YS4gVm9j77+9IHRhbWLvv71tIGRlY2xhcmEgcXVlIG8gZGVw77+9c2l0byBkZSBzdWEgcHVibGljYe+/ve+/vW8gbu+/vW8sIHF1ZSBzZWphIGRlIHNldSBjb25oZWNpbWVudG8sIGluZnJpbmdlIGRpcmVpdG9zIGF1dG9yYWlzIGRlIG5pbmd177+9bS4KCkNhc28gYSBzdWEgcHVibGljYe+/ve+/vW8gY29udGVuaGEgbWF0ZXJpYWwgcXVlIHZvY++/vSBu77+9byBwb3NzdWkgYSB0aXR1bGFyaWRhZGUgZG9zIGRpcmVpdG9zIGF1dG9yYWlzLCB2b2Pvv70gZGVjbGFyYSBxdWUgb2J0ZXZlIGEgcGVybWlzc++/vW8gaXJyZXN0cml0YSBkbyBkZXRlbnRvciBkb3MgZGlyZWl0b3MgYXV0b3JhaXMgcGFyYSBjb25jZWRlciBhbyBSZXBvc2l077+9cmlvIEluc3RpdHVjaW9uYWwgZGEgVUZNRyBvcyBkaXJlaXRvcyBhcHJlc2VudGFkb3MgbmVzdGEgbGljZW7vv71hLCBlIHF1ZSBlc3NlIG1hdGVyaWFsIGRlIHByb3ByaWVkYWRlIGRlIHRlcmNlaXJvcyBlc3Tvv70gY2xhcmFtZW50ZSBpZGVudGlmaWNhZG8gZSByZWNvbmhlY2lkbyBubyB0ZXh0byBvdSBubyBjb250Ze+/vWRvIGRhIHB1YmxpY2Hvv73vv71vIG9yYSBkZXBvc2l0YWRhLgoKQ0FTTyBBIFBVQkxJQ0Hvv73vv71PIE9SQSBERVBPU0lUQURBIFRFTkhBIFNJRE8gUkVTVUxUQURPIERFIFVNIFBBVFJPQ++/vU5JTyBPVSBBUE9JTyBERSBVTUEgQUfvv71OQ0lBIERFIEZPTUVOVE8gT1UgT1VUUk8gT1JHQU5JU01PLCBWT0Pvv70gREVDTEFSQSBRVUUgUkVTUEVJVE9VIFRPRE9TIEUgUVVBSVNRVUVSIERJUkVJVE9TIERFIFJFVklT77+9TyBDT01PIFRBTULvv71NIEFTIERFTUFJUyBPQlJJR0Hvv73vv71FUyBFWElHSURBUyBQT1IgQ09OVFJBVE8gT1UgQUNPUkRPLgoKTyBSZXBvc2l077+9cmlvIEluc3RpdHVjaW9uYWwgZGEgVUZNRyBzZSBjb21wcm9tZXRlIGEgaWRlbnRpZmljYXIgY2xhcmFtZW50ZSBvIHNldSBub21lKHMpIG91IG8ocykgbm9tZXMocykgZG8ocykgZGV0ZW50b3IoZXMpIGRvcyBkaXJlaXRvcyBhdXRvcmFpcyBkYSBwdWJsaWNh77+977+9bywgZSBu77+9byBmYXLvv70gcXVhbHF1ZXIgYWx0ZXJh77+977+9bywgYWzvv71tIGRhcXVlbGFzIGNvbmNlZGlkYXMgcG9yIGVzdGEgbGljZW7vv71hLgo=Repositório de PublicaçõesPUBhttps://repositorio.ufmg.br/oaiopendoar:2022-05-12T11:59:30Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)false |
dc.title.pt_BR.fl_str_mv |
Anthelmintic activity of Annona crassiflora leaves against Haemonchus contortus: part 1: in vitroinhibition of the hatchability and larval development |
dc.title.alternative.pt_BR.fl_str_mv |
Atividade antihelmíntica de folhas de Annona crassiflora contra Haemonchus contortus: parte 1: inibição in vitro da eclodibilidade e do desenvolvimento larval |
title |
Anthelmintic activity of Annona crassiflora leaves against Haemonchus contortus: part 1: in vitroinhibition of the hatchability and larval development |
spellingShingle |
Anthelmintic activity of Annona crassiflora leaves against Haemonchus contortus: part 1: in vitroinhibition of the hatchability and larval development Marco Antônio Martins Caldeira Haemonchus contortus Plantas - Nematódeos Flavonoides |
title_short |
Anthelmintic activity of Annona crassiflora leaves against Haemonchus contortus: part 1: in vitroinhibition of the hatchability and larval development |
title_full |
Anthelmintic activity of Annona crassiflora leaves against Haemonchus contortus: part 1: in vitroinhibition of the hatchability and larval development |
title_fullStr |
Anthelmintic activity of Annona crassiflora leaves against Haemonchus contortus: part 1: in vitroinhibition of the hatchability and larval development |
title_full_unstemmed |
Anthelmintic activity of Annona crassiflora leaves against Haemonchus contortus: part 1: in vitroinhibition of the hatchability and larval development |
title_sort |
Anthelmintic activity of Annona crassiflora leaves against Haemonchus contortus: part 1: in vitroinhibition of the hatchability and larval development |
author |
Marco Antônio Martins Caldeira |
author_facet |
Marco Antônio Martins Caldeira Franciellen Morais Costa Kaike Magno de Macêdo Valdo Soares Martins Júnior Dheison Emanuel Augusto Pereira Ana Claúdia Maia Soares Isabela Parolis Martins Idael Matheus Góes Lopes Fernão Castro Braga Neide Judith Faria de Oliveira Eduardo Robson Duarte |
author_role |
author |
author2 |
Franciellen Morais Costa Kaike Magno de Macêdo Valdo Soares Martins Júnior Dheison Emanuel Augusto Pereira Ana Claúdia Maia Soares Isabela Parolis Martins Idael Matheus Góes Lopes Fernão Castro Braga Neide Judith Faria de Oliveira Eduardo Robson Duarte |
author2_role |
author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Marco Antônio Martins Caldeira Franciellen Morais Costa Kaike Magno de Macêdo Valdo Soares Martins Júnior Dheison Emanuel Augusto Pereira Ana Claúdia Maia Soares Isabela Parolis Martins Idael Matheus Góes Lopes Fernão Castro Braga Neide Judith Faria de Oliveira Eduardo Robson Duarte |
dc.subject.other.pt_BR.fl_str_mv |
Haemonchus contortus Plantas - Nematódeos Flavonoides |
topic |
Haemonchus contortus Plantas - Nematódeos Flavonoides |
description |
We evaluated the activity of Annona crassiflora leaves against Haemonchus contortus resistant to albendazol. Aqueous (AE), ethanolic (EE) and ethyl acetate (EAE) extracts were produced and the predominant presence of flavonoids was observed in HPLC-DAD chromatograms. Initially we evaluated the larval development inhibition (LDI) of dry A. crassiflora leaf powder or its AE directly in fecal quantitative cultures. The efficacies of the extracts, with or without tannins, on egg hatching inhibition (EHI) were investigated reveling that the EE was the most effective (LC90 = 8.96 mg/mL). However, after tannin removal, AE showed the highest activity (LC90 = 4.27 mg/mL). In the LDI test, the LC90 of AE was < 6.25 mg/g of fecal culture and to leaf powder of leaves was 69.14 mg/g. High efficacies of AE and EE for EHI were detected and the tannins were not the main active metabolites. The anthelmintic potential of this plant could be attributed to association between flavonoids and other metabolites. |
publishDate |
2019 |
dc.date.issued.fl_str_mv |
2019-07 |
dc.date.accessioned.fl_str_mv |
2022-05-12T11:59:30Z |
dc.date.available.fl_str_mv |
2022-05-12T11:59:30Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1843/41599 |
dc.identifier.doi.pt_BR.fl_str_mv |
https://doi.org/10.26605/medvet-v13n2-3067 |
dc.identifier.issn.pt_BR.fl_str_mv |
1809-4678 |
url |
https://doi.org/10.26605/medvet-v13n2-3067 http://hdl.handle.net/1843/41599 |
identifier_str_mv |
1809-4678 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.ispartof.pt_BR.fl_str_mv |
Medicina Veterinária |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Universidade Federal de Minas Gerais |
dc.publisher.initials.fl_str_mv |
UFMG |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
ICA - INSTITUTO DE CIÊNCIAS AGRÁRIAS |
publisher.none.fl_str_mv |
Universidade Federal de Minas Gerais |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFMG instname:Universidade Federal de Minas Gerais (UFMG) instacron:UFMG |
instname_str |
Universidade Federal de Minas Gerais (UFMG) |
instacron_str |
UFMG |
institution |
UFMG |
reponame_str |
Repositório Institucional da UFMG |
collection |
Repositório Institucional da UFMG |
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