Honey activity on the proliferation and migration of oral squamous cell carcinoma: in vitro and bioinformatic analysis
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFMG |
Texto Completo: | https://doi.org/10.5897/SRE2018.6590 http://hdl.handle.net/1843/42348 https://orcid.org/ 0000-0001-9836-4117 |
Resumo: | This study aimed to investigate, in oral squamous carcinoma cells (OSCC), the antineoplastic effect of Apis mellifera honey produced from Aroeira and wildflower, in the region of Jequitinhonha River Valley, Brazil. Furthermore, we investigate the molecular mechanism of honey performance through bioinformatic analysis. Honey and OSCC-related gene list were screened using GeneCards. The online tool STRING was used to identify the potential interaction networks of protein products of these genes. The genes with the highest weighted number of links (WNL) were called leader genes. GO and KEGG pathway analysis was performed to classify the genes. We use a search tool STITCH for interactions of chemicals-proteins. The chemical characterization of honey and its antiproliferative and antimigratory effects in SCC-9 cell lines was evaluated in vitro. The analysis of the interaction network obtained from the STRING database indicated by the following leader genes: TNF, IL6 and HDAC1. GO analysis for the honey, and OSCC-related proteins revealed that leader genes-regulated cancers genes were markedly enriched in some functions. Through the chemical characterization, the bioactive classes: steroids/triterpenoids, flavonoids, saponins, alkaloids, and tannins were identified. Polyphenols act in activating CASP3 and TP53 and inhibit the action of DNMT1. The honey produced from Aroeira and wildflower reduced proliferative phenotype and migration of OSCC cells. |
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2022-06-08T12:31:27Z2022-06-08T12:31:27Z20181315158171https://doi.org/10.5897/SRE2018.65901992-2248http://hdl.handle.net/1843/42348https://orcid.org/ 0000-0001-9836-4117This study aimed to investigate, in oral squamous carcinoma cells (OSCC), the antineoplastic effect of Apis mellifera honey produced from Aroeira and wildflower, in the region of Jequitinhonha River Valley, Brazil. Furthermore, we investigate the molecular mechanism of honey performance through bioinformatic analysis. Honey and OSCC-related gene list were screened using GeneCards. The online tool STRING was used to identify the potential interaction networks of protein products of these genes. The genes with the highest weighted number of links (WNL) were called leader genes. GO and KEGG pathway analysis was performed to classify the genes. We use a search tool STITCH for interactions of chemicals-proteins. The chemical characterization of honey and its antiproliferative and antimigratory effects in SCC-9 cell lines was evaluated in vitro. The analysis of the interaction network obtained from the STRING database indicated by the following leader genes: TNF, IL6 and HDAC1. GO analysis for the honey, and OSCC-related proteins revealed that leader genes-regulated cancers genes were markedly enriched in some functions. Through the chemical characterization, the bioactive classes: steroids/triterpenoids, flavonoids, saponins, alkaloids, and tannins were identified. Polyphenols act in activating CASP3 and TP53 and inhibit the action of DNMT1. The honey produced from Aroeira and wildflower reduced proliferative phenotype and migration of OSCC cells.engUniversidade Federal de Minas GeraisUFMGBrasilICA - INSTITUTO DE CIÊNCIAS AGRÁRIASScientific research and essaysMel de abelha -- Jequitinhonha, Rio, Vale (MG e BA)Aroeira do sertãoAbelha-européiaMel -- Uso terapêuticoHoney activity on the proliferation and migration of oral squamous cell carcinoma: in vitro and bioinformatic analysisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttps://academicjournals.org/journal/SRE/article-abstract/60EC8D659093iEliane Macedo Sobrinho SantosKattyanne de Souza CostaAndre Luiz Sena GuimaraesLucyana Conceiçao FariasHercules Otacílio SantosJuliana Rezende Sá Miranda GoncalvesAnna Christina de AlmeidaIgor Viana BrandiAlex Sander Rodrigues CangussuJanainne Nunes AlvesSabrina Ferreira de JesusRicardo Jardim Neivainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMGinstname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMGLICENSELicense.txtLicense.txttext/plain; charset=utf-82042https://repositorio.ufmg.br/bitstream/1843/42348/1/License.txtfa505098d172de0bc8864fc1287ffe22MD51ORIGINALHoney activity on the proliferation and migration of oral squamous cell carcinoma in vitro and bioinformatic analysis.pdfHoney activity on the proliferation and migration of oral squamous cell carcinoma in vitro and bioinformatic analysis.pdfapplication/pdf470155https://repositorio.ufmg.br/bitstream/1843/42348/2/Honey%20activity%20on%20the%20proliferation%20and%20migration%20of%20oral%20squamous%20cell%20carcinoma%20in%20vitro%20and%20bioinformatic%20analysis.pdfed86f829067911305e7beea872bd76a9MD521843/423482022-06-08 09:31:27.666oai:repositorio.ufmg.br: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Repositório de PublicaçõesPUBhttps://repositorio.ufmg.br/oaiopendoar:2022-06-08T12:31:27Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)false |
dc.title.pt_BR.fl_str_mv |
Honey activity on the proliferation and migration of oral squamous cell carcinoma: in vitro and bioinformatic analysis |
title |
Honey activity on the proliferation and migration of oral squamous cell carcinoma: in vitro and bioinformatic analysis |
spellingShingle |
Honey activity on the proliferation and migration of oral squamous cell carcinoma: in vitro and bioinformatic analysis Eliane Macedo Sobrinho Santos Mel de abelha -- Jequitinhonha, Rio, Vale (MG e BA) Aroeira do sertão Abelha-européia Mel -- Uso terapêutico |
title_short |
Honey activity on the proliferation and migration of oral squamous cell carcinoma: in vitro and bioinformatic analysis |
title_full |
Honey activity on the proliferation and migration of oral squamous cell carcinoma: in vitro and bioinformatic analysis |
title_fullStr |
Honey activity on the proliferation and migration of oral squamous cell carcinoma: in vitro and bioinformatic analysis |
title_full_unstemmed |
Honey activity on the proliferation and migration of oral squamous cell carcinoma: in vitro and bioinformatic analysis |
title_sort |
Honey activity on the proliferation and migration of oral squamous cell carcinoma: in vitro and bioinformatic analysis |
author |
Eliane Macedo Sobrinho Santos |
author_facet |
Eliane Macedo Sobrinho Santos Kattyanne de Souza Costa Andre Luiz Sena Guimaraes Lucyana Conceiçao Farias Hercules Otacílio Santos Juliana Rezende Sá Miranda Goncalves Anna Christina de Almeida Igor Viana Brandi Alex Sander Rodrigues Cangussu Janainne Nunes Alves Sabrina Ferreira de Jesus Ricardo Jardim Neiva |
author_role |
author |
author2 |
Kattyanne de Souza Costa Andre Luiz Sena Guimaraes Lucyana Conceiçao Farias Hercules Otacílio Santos Juliana Rezende Sá Miranda Goncalves Anna Christina de Almeida Igor Viana Brandi Alex Sander Rodrigues Cangussu Janainne Nunes Alves Sabrina Ferreira de Jesus Ricardo Jardim Neiva |
author2_role |
author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Eliane Macedo Sobrinho Santos Kattyanne de Souza Costa Andre Luiz Sena Guimaraes Lucyana Conceiçao Farias Hercules Otacílio Santos Juliana Rezende Sá Miranda Goncalves Anna Christina de Almeida Igor Viana Brandi Alex Sander Rodrigues Cangussu Janainne Nunes Alves Sabrina Ferreira de Jesus Ricardo Jardim Neiva |
dc.subject.other.pt_BR.fl_str_mv |
Mel de abelha -- Jequitinhonha, Rio, Vale (MG e BA) Aroeira do sertão Abelha-européia Mel -- Uso terapêutico |
topic |
Mel de abelha -- Jequitinhonha, Rio, Vale (MG e BA) Aroeira do sertão Abelha-européia Mel -- Uso terapêutico |
description |
This study aimed to investigate, in oral squamous carcinoma cells (OSCC), the antineoplastic effect of Apis mellifera honey produced from Aroeira and wildflower, in the region of Jequitinhonha River Valley, Brazil. Furthermore, we investigate the molecular mechanism of honey performance through bioinformatic analysis. Honey and OSCC-related gene list were screened using GeneCards. The online tool STRING was used to identify the potential interaction networks of protein products of these genes. The genes with the highest weighted number of links (WNL) were called leader genes. GO and KEGG pathway analysis was performed to classify the genes. We use a search tool STITCH for interactions of chemicals-proteins. The chemical characterization of honey and its antiproliferative and antimigratory effects in SCC-9 cell lines was evaluated in vitro. The analysis of the interaction network obtained from the STRING database indicated by the following leader genes: TNF, IL6 and HDAC1. GO analysis for the honey, and OSCC-related proteins revealed that leader genes-regulated cancers genes were markedly enriched in some functions. Through the chemical characterization, the bioactive classes: steroids/triterpenoids, flavonoids, saponins, alkaloids, and tannins were identified. Polyphenols act in activating CASP3 and TP53 and inhibit the action of DNMT1. The honey produced from Aroeira and wildflower reduced proliferative phenotype and migration of OSCC cells. |
publishDate |
2018 |
dc.date.issued.fl_str_mv |
2018 |
dc.date.accessioned.fl_str_mv |
2022-06-08T12:31:27Z |
dc.date.available.fl_str_mv |
2022-06-08T12:31:27Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1843/42348 |
dc.identifier.doi.pt_BR.fl_str_mv |
https://doi.org/10.5897/SRE2018.6590 |
dc.identifier.issn.pt_BR.fl_str_mv |
1992-2248 |
dc.identifier.orcid.pt_BR.fl_str_mv |
https://orcid.org/ 0000-0001-9836-4117 |
url |
https://doi.org/10.5897/SRE2018.6590 http://hdl.handle.net/1843/42348 https://orcid.org/ 0000-0001-9836-4117 |
identifier_str_mv |
1992-2248 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
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Scientific research and essays |
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info:eu-repo/semantics/openAccess |
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openAccess |
dc.publisher.none.fl_str_mv |
Universidade Federal de Minas Gerais |
dc.publisher.initials.fl_str_mv |
UFMG |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
ICA - INSTITUTO DE CIÊNCIAS AGRÁRIAS |
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Universidade Federal de Minas Gerais |
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reponame:Repositório Institucional da UFMG instname:Universidade Federal de Minas Gerais (UFMG) instacron:UFMG |
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Universidade Federal de Minas Gerais (UFMG) |
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UFMG |
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UFMG |
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Repositório Institucional da UFMG |
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