Honey activity on the proliferation and migration of oral squamous cell carcinoma: in vitro and bioinformatic analysis

• Autor(a) principal: Eliane Macedo Sobrinho Santos
• Data de Publicação: 2018
• Outros Autores: Kattyanne de Souza Costa, Andre Luiz Sena Guimaraes, Lucyana Conceiçao Farias, Hercules Otacílio Santos, Juliana Rezende Sá Miranda Goncalves, Anna Christina de Almeida, Igor Viana Brandi, Alex Sander Rodrigues Cangussu, Janainne Nunes Alves, Sabrina Ferreira de Jesus, Ricardo Jardim Neiva
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UFMG
• Texto Completo: https://doi.org/10.5897/SRE2018.6590; http://hdl.handle.net/1843/42348; https://orcid.org/ 0000-0001-9836-4117
• topic: Mel de abelha -- Jequitinhonha, Rio, Vale (MG e BA); Aroeira do sertão; Abelha-européia; Mel -- Uso terapêutico

This study aimed to investigate, in oral squamous carcinoma cells (OSCC), the antineoplastic effect of Apis mellifera honey produced from Aroeira and wildflower, in the region of Jequitinhonha River Valley, Brazil. Furthermore, we investigate the molecular mechanism of honey performance through bioinformatic analysis. Honey and OSCC-related gene list were screened using GeneCards. The online tool STRING was used to identify the potential interaction networks of protein products of these genes. The genes with the highest weighted number of links (WNL) were called leader genes. GO and KEGG pathway analysis was performed to classify the genes. We use a search tool STITCH for interactions of chemicals-proteins. The chemical characterization of honey and its antiproliferative and antimigratory effects in SCC-9 cell lines was evaluated in vitro. The analysis of the interaction network obtained from the STRING database indicated by the following leader genes: TNF, IL6 and HDAC1. GO analysis for the honey, and OSCC-related proteins revealed that leader genes-regulated cancers genes were markedly enriched in some functions. Through the chemical characterization, the bioactive classes: steroids/triterpenoids, flavonoids, saponins, alkaloids, and tannins were identified. Polyphenols act in activating CASP3 and TP53 and inhibit the action of DNMT1. The honey produced from Aroeira and wildflower reduced proliferative phenotype and migration of OSCC cells.