Chronic lymphocytic leukemia (cll): evaluation of akt protein kinase and microrna gene expression related to disease pathogenesis
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFMG |
Texto Completo: | https://doi.org/10.1590/s2175-97902022e19946 http://hdl.handle.net/1843/61362 http://orcid.org/0000-0002-9024-8872 |
Resumo: | The present study evaluated 56 patients diagnosed with Chronic Lymphocytic Leukemia (CLL) and a control group of 44 clinically healthy subjects with no previous history of leukemia. Genetic expressions of AKT and microRNAs were evaluated by quantitative PCR (qPCR). A significant increase in AKT gene expression in patients when compared to controls was observed (p = 0.017). When the patients were stratified according to Binet subgroups, a significant difference was observed between the subgroups, with this protein kinase appearing more expressed in the B+C subgroup (p = 0.013). Regarding miRNA expression, miR-let-7b and miR-26a were reduced in CLL patients, when compared to controls. However, no significant differences were observed in these microRNA expressions between the Binet subgroups (A versus B+C). By contrast, miR-21 to miR-27a oncogenes showed no expression difference between CLL patients and controls. AKT protein kinase is involved in the signaling cascade that occurs with BCR receptor activation, leading to increased lymphocyte survival and protection against the induction of cell death in CLL. Thus, increased AKT protein kinase expression and the reduction of miR-let-7b and miR-26a, both tumor suppressors, may explain increased lymphocyte survival in CLL patients and may be promising markers for the prognostic evaluation of this disease. |
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2023-11-24T19:34:32Z2023-11-24T19:34:32Z202258https://doi.org/10.1590/s2175-97902022e199462175-9790http://hdl.handle.net/1843/61362http://orcid.org/0000-0002-9024-8872The present study evaluated 56 patients diagnosed with Chronic Lymphocytic Leukemia (CLL) and a control group of 44 clinically healthy subjects with no previous history of leukemia. Genetic expressions of AKT and microRNAs were evaluated by quantitative PCR (qPCR). A significant increase in AKT gene expression in patients when compared to controls was observed (p = 0.017). When the patients were stratified according to Binet subgroups, a significant difference was observed between the subgroups, with this protein kinase appearing more expressed in the B+C subgroup (p = 0.013). Regarding miRNA expression, miR-let-7b and miR-26a were reduced in CLL patients, when compared to controls. However, no significant differences were observed in these microRNA expressions between the Binet subgroups (A versus B+C). By contrast, miR-21 to miR-27a oncogenes showed no expression difference between CLL patients and controls. AKT protein kinase is involved in the signaling cascade that occurs with BCR receptor activation, leading to increased lymphocyte survival and protection against the induction of cell death in CLL. Thus, increased AKT protein kinase expression and the reduction of miR-let-7b and miR-26a, both tumor suppressors, may explain increased lymphocyte survival in CLL patients and may be promising markers for the prognostic evaluation of this disease.O presente estudo avaliou 56 pacientes com diagnóstico de Leucemia Linfocítica Crônica (LLC) e um grupo controle de 44 indivíduos clinicamente saudáveis, sem história prévia de leucemia. As expressões genéticas de AKT e microRNAs foram avaliadas por PCR quantitativo (qPCR). Foi observado aumento significativo na expressão do gene AKT em pacientes quando comparados aos controles (p = 0,017). Quando os pacientes foram estratificados de acordo com os subgrupos de Binet, foi observada diferença significativa entre os subgrupos, com esta proteína quinase aparecendo mais expressa no subgrupo B+C (p = 0,013). Em relação à expressão de miRNA, miR-let-7b e miR-26a foram reduzidos em pacientes com LLC, quando comparados aos controles. No entanto, não foram observadas diferenças significativas nestas expressões de microRNA entre os subgrupos Binet (A versus B+C). Por outro lado, os oncogenes miR-21 a miR-27a não mostraram diferença de expressão entre pacientes com LLC e controles. A proteína quinase AKT está envolvida na cascata de sinalização que ocorre com a ativação do receptor BCR, levando ao aumento da sobrevivência dos linfócitos e à proteção contra a indução da morte celular na LLC. Assim, o aumento da expressão da proteína quinase AKT e a redução de miR-let-7b e miR-26a, ambos supressores tumorais, podem explicar o aumento da sobrevida de linfócitos em pacientes com LLC e podem ser marcadores promissores para a avaliação prognóstica desta doença.CNPq - Conselho Nacional de Desenvolvimento Científico e TecnológicoFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas GeraisCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível SuperiorengUniversidade Federal de Minas GeraisUFMGBrasilFAR - DEPARTAMENTO DE ANÁLISES CLÍNICAS E TOXICOLÓGICASHCL - HOSPITAL DAS CLINICASICX - DEPARTAMENTO DE ESTATÍSTICABrazilian Journal of Pharmaceutical SciencesLeucemia linfocítica crônica de células BProteína quinase BMicroRNAsApoptoseChronic lymphocytic leukemiaAKT protein kinasemicroRNAsApoptosisChronic lymphocytic leukemia (cll): evaluation of akt protein kinase and microrna gene expression related to disease pathogenesisChronic Lymphocytic Leukemia (CLL): evaluation of AKT protein kinase and microRNA gene expression related to disease pathogenesisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttps://www.scielo.br/j/bjps/a/n7phmZ5SLKh3R4Z6hSLzMvv/abstract/?lang=en#Lorena Caixeta GomesRodrigo Ribeiro ResendeRicardo Cambraia ParreiraCláudia Natália FerreriaEdna Afonso ReisRita Carolina Figueiredo DuarteLuan Carlos Vieira AlvesSérgio Schusterschitz da Silva AraújoMaria das Gracas CarvalhoAdriano de Paula Sabinoapplication/pdfinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMGinstname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMGLICENSELicense.txtLicense.txttext/plain; charset=utf-82042https://repositorio.ufmg.br/bitstream/1843/61362/1/License.txtfa505098d172de0bc8864fc1287ffe22MD51ORIGINALChronic Lymphocytic Leukemia (CLL)_ evaluation of AKT protein kinase and microRNA gene expression related to disease pathogenesis.pdfChronic Lymphocytic Leukemia (CLL)_ evaluation of AKT protein kinase and microRNA gene expression related to disease pathogenesis.pdfapplication/pdf688415https://repositorio.ufmg.br/bitstream/1843/61362/2/Chronic%20Lymphocytic%20Leukemia%20%28CLL%29_%20evaluation%20of%20AKT%20protein%20kinase%20and%20microRNA%20gene%20expression%20related%20to%20disease%20pathogenesis.pdfdf2c7d804b3b34b9f8147d243e6a229bMD521843/613622023-11-24 16:34:32.408oai:repositorio.ufmg.br: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Repositório de PublicaçõesPUBhttps://repositorio.ufmg.br/oaiopendoar:2023-11-24T19:34:32Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)false |
dc.title.pt_BR.fl_str_mv |
Chronic lymphocytic leukemia (cll): evaluation of akt protein kinase and microrna gene expression related to disease pathogenesis |
dc.title.alternative.pt_BR.fl_str_mv |
Chronic Lymphocytic Leukemia (CLL): evaluation of AKT protein kinase and microRNA gene expression related to disease pathogenesis |
title |
Chronic lymphocytic leukemia (cll): evaluation of akt protein kinase and microrna gene expression related to disease pathogenesis |
spellingShingle |
Chronic lymphocytic leukemia (cll): evaluation of akt protein kinase and microrna gene expression related to disease pathogenesis Lorena Caixeta Gomes Chronic lymphocytic leukemia AKT protein kinase microRNAs Apoptosis Leucemia linfocítica crônica de células B Proteína quinase B MicroRNAs Apoptose |
title_short |
Chronic lymphocytic leukemia (cll): evaluation of akt protein kinase and microrna gene expression related to disease pathogenesis |
title_full |
Chronic lymphocytic leukemia (cll): evaluation of akt protein kinase and microrna gene expression related to disease pathogenesis |
title_fullStr |
Chronic lymphocytic leukemia (cll): evaluation of akt protein kinase and microrna gene expression related to disease pathogenesis |
title_full_unstemmed |
Chronic lymphocytic leukemia (cll): evaluation of akt protein kinase and microrna gene expression related to disease pathogenesis |
title_sort |
Chronic lymphocytic leukemia (cll): evaluation of akt protein kinase and microrna gene expression related to disease pathogenesis |
author |
Lorena Caixeta Gomes |
author_facet |
Lorena Caixeta Gomes Rodrigo Ribeiro Resende Ricardo Cambraia Parreira Cláudia Natália Ferreria Edna Afonso Reis Rita Carolina Figueiredo Duarte Luan Carlos Vieira Alves Sérgio Schusterschitz da Silva Araújo Maria das Gracas Carvalho Adriano de Paula Sabino |
author_role |
author |
author2 |
Rodrigo Ribeiro Resende Ricardo Cambraia Parreira Cláudia Natália Ferreria Edna Afonso Reis Rita Carolina Figueiredo Duarte Luan Carlos Vieira Alves Sérgio Schusterschitz da Silva Araújo Maria das Gracas Carvalho Adriano de Paula Sabino |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Lorena Caixeta Gomes Rodrigo Ribeiro Resende Ricardo Cambraia Parreira Cláudia Natália Ferreria Edna Afonso Reis Rita Carolina Figueiredo Duarte Luan Carlos Vieira Alves Sérgio Schusterschitz da Silva Araújo Maria das Gracas Carvalho Adriano de Paula Sabino |
dc.subject.por.fl_str_mv |
Chronic lymphocytic leukemia AKT protein kinase microRNAs Apoptosis |
topic |
Chronic lymphocytic leukemia AKT protein kinase microRNAs Apoptosis Leucemia linfocítica crônica de células B Proteína quinase B MicroRNAs Apoptose |
dc.subject.other.pt_BR.fl_str_mv |
Leucemia linfocítica crônica de células B Proteína quinase B MicroRNAs Apoptose |
description |
The present study evaluated 56 patients diagnosed with Chronic Lymphocytic Leukemia (CLL) and a control group of 44 clinically healthy subjects with no previous history of leukemia. Genetic expressions of AKT and microRNAs were evaluated by quantitative PCR (qPCR). A significant increase in AKT gene expression in patients when compared to controls was observed (p = 0.017). When the patients were stratified according to Binet subgroups, a significant difference was observed between the subgroups, with this protein kinase appearing more expressed in the B+C subgroup (p = 0.013). Regarding miRNA expression, miR-let-7b and miR-26a were reduced in CLL patients, when compared to controls. However, no significant differences were observed in these microRNA expressions between the Binet subgroups (A versus B+C). By contrast, miR-21 to miR-27a oncogenes showed no expression difference between CLL patients and controls. AKT protein kinase is involved in the signaling cascade that occurs with BCR receptor activation, leading to increased lymphocyte survival and protection against the induction of cell death in CLL. Thus, increased AKT protein kinase expression and the reduction of miR-let-7b and miR-26a, both tumor suppressors, may explain increased lymphocyte survival in CLL patients and may be promising markers for the prognostic evaluation of this disease. |
publishDate |
2022 |
dc.date.issued.fl_str_mv |
2022 |
dc.date.accessioned.fl_str_mv |
2023-11-24T19:34:32Z |
dc.date.available.fl_str_mv |
2023-11-24T19:34:32Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1843/61362 |
dc.identifier.doi.pt_BR.fl_str_mv |
https://doi.org/10.1590/s2175-97902022e19946 |
dc.identifier.issn.pt_BR.fl_str_mv |
2175-9790 |
dc.identifier.orcid.pt_BR.fl_str_mv |
http://orcid.org/0000-0002-9024-8872 |
url |
https://doi.org/10.1590/s2175-97902022e19946 http://hdl.handle.net/1843/61362 http://orcid.org/0000-0002-9024-8872 |
identifier_str_mv |
2175-9790 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Brazilian Journal of Pharmaceutical Sciences |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Minas Gerais |
dc.publisher.initials.fl_str_mv |
UFMG |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
FAR - DEPARTAMENTO DE ANÁLISES CLÍNICAS E TOXICOLÓGICAS HCL - HOSPITAL DAS CLINICAS ICX - DEPARTAMENTO DE ESTATÍSTICA |
publisher.none.fl_str_mv |
Universidade Federal de Minas Gerais |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFMG instname:Universidade Federal de Minas Gerais (UFMG) instacron:UFMG |
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Universidade Federal de Minas Gerais (UFMG) |
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UFMG |
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UFMG |
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Repositório Institucional da UFMG |
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Repositório Institucional da UFMG |
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