Single-laboratory validation of a method for detection of Roundup Ready soy in soybeans: application of new strategies for qualitative validation

Detalhes bibliográficos
Autor(a) principal: Carolina Sheng Whei Miaw
Data de Publicação: 2017
Outros Autores: Gláucia Celeste de Souza Amâncio, Viviane Netto Rocha, Jovita Eugênia Gazzinelli Cruz Madeira, Scheilla Vitorino Carvalho de Souza
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFMG
Texto Completo: http://hdl.handle.net/1843/40515
Resumo: CNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
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spelling 2022-03-28T20:34:11Z2022-03-28T20:34:11Z20179110511410.3920/QAS2015.073917578361http://hdl.handle.net/1843/40515CNPq - Conselho Nacional de Desenvolvimento Científico e TecnológicoConsidering expansion of genetically modified organisms and the basic principle of consumers’ right to access information about products, legislations of several countries establish a limit for labelling transgenic food. Qualitative tests based on the polymerase chain reaction (PCR) have been recommended. However, validation of qualitative methods is still a critical point in the quality management of food analysis laboratories. A screening method for the detection of Roundup Ready (RR) soy in soybeans by nested PCR was validated by a novel qualitative approach, in a single-laboratory, considering all fundamental parameters for an adequate evaluation of fitness for purpose. Blank samples of soybeans and formulations containing 0.001 to 1% of RR soy were analysed. Agarose gel electrophoresis and fluorimetry techniques were compared in terms of the quantification of extracted DNA. False-positive rate obtained was 0%, with selectivity and reliability rates of 100.0% for both techniques. Sensitivity and reliability rates varied between 23.3 and 100.0% (agarose gel) and between 30.0 and 100.0% (fluorimetry), respectively. Levels above 0.030% presented 100.0% positive results. Unreliable regions were estimated by non-linear models, and the calculated detection limits were 0.0067 and 0.0047%, for agarose gel electrophoresis and fluorimetry, respectively. Accordance and concordance values of 1.0 were obtained for levels near the regulated limit. The method was considered fit for screening purposes. Analysis of commercial samples demonstrated the applicability of the method and the compliance with Brazilian legislation.engUniversidade Federal de Minas GeraisUFMGBrasilFAR - DEPARTAMENTO DE ALIMENTOSQuality Assurance and Safety of Crops & FoodsTecnologia de alimentosSojaGenetically modified organismsRoundup Ready soyScreening methodLabelling regulationsSingle-laboratory validationRotulagemSingle-laboratory validation of a method for detection of Roundup Ready soy in soybeans: application of new strategies for qualitative validationinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttps://www.qascf.com/index.php/qas/article/view/185Carolina Sheng Whei MiawGláucia Celeste de Souza AmâncioViviane Netto RochaJovita Eugênia Gazzinelli Cruz MadeiraScheilla Vitorino Carvalho de Souzaapplication/pdfinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMGinstname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMGLICENSELicense.txtLicense.txttext/plain; charset=utf-82042https://repositorio.ufmg.br/bitstream/1843/40515/1/License.txtfa505098d172de0bc8864fc1287ffe22MD51ORIGINALSingle-laboratory validation of a method for detection of Roundup Ready soy in soybeans application of new strategies for qualitative validation.pdfSingle-laboratory validation of a method for detection of Roundup Ready soy in soybeans application of new strategies for qualitative validation.pdfapplication/pdf331926https://repositorio.ufmg.br/bitstream/1843/40515/2/Single-laboratory%20validation%20of%20a%20method%20for%20detection%20of%20Roundup%20Ready%20soy%20in%20soybeans%20application%20of%20new%20strategies%20for%20qualitative%20validation.pdfcfa8d12bddea6cd21aad220047545e31MD521843/405152022-03-28 17:34:11.42oai:repositorio.ufmg.br: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Repositório de PublicaçõesPUBhttps://repositorio.ufmg.br/oaiopendoar:2022-03-28T20:34:11Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)false
dc.title.pt_BR.fl_str_mv Single-laboratory validation of a method for detection of Roundup Ready soy in soybeans: application of new strategies for qualitative validation
title Single-laboratory validation of a method for detection of Roundup Ready soy in soybeans: application of new strategies for qualitative validation
spellingShingle Single-laboratory validation of a method for detection of Roundup Ready soy in soybeans: application of new strategies for qualitative validation
Carolina Sheng Whei Miaw
Genetically modified organisms
Roundup Ready soy
Screening method
Labelling regulations
Single-laboratory validation
Rotulagem
Tecnologia de alimentos
Soja
title_short Single-laboratory validation of a method for detection of Roundup Ready soy in soybeans: application of new strategies for qualitative validation
title_full Single-laboratory validation of a method for detection of Roundup Ready soy in soybeans: application of new strategies for qualitative validation
title_fullStr Single-laboratory validation of a method for detection of Roundup Ready soy in soybeans: application of new strategies for qualitative validation
title_full_unstemmed Single-laboratory validation of a method for detection of Roundup Ready soy in soybeans: application of new strategies for qualitative validation
title_sort Single-laboratory validation of a method for detection of Roundup Ready soy in soybeans: application of new strategies for qualitative validation
author Carolina Sheng Whei Miaw
author_facet Carolina Sheng Whei Miaw
Gláucia Celeste de Souza Amâncio
Viviane Netto Rocha
Jovita Eugênia Gazzinelli Cruz Madeira
Scheilla Vitorino Carvalho de Souza
author_role author
author2 Gláucia Celeste de Souza Amâncio
Viviane Netto Rocha
Jovita Eugênia Gazzinelli Cruz Madeira
Scheilla Vitorino Carvalho de Souza
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Carolina Sheng Whei Miaw
Gláucia Celeste de Souza Amâncio
Viviane Netto Rocha
Jovita Eugênia Gazzinelli Cruz Madeira
Scheilla Vitorino Carvalho de Souza
dc.subject.por.fl_str_mv Genetically modified organisms
Roundup Ready soy
Screening method
Labelling regulations
Single-laboratory validation
Rotulagem
topic Genetically modified organisms
Roundup Ready soy
Screening method
Labelling regulations
Single-laboratory validation
Rotulagem
Tecnologia de alimentos
Soja
dc.subject.other.pt_BR.fl_str_mv Tecnologia de alimentos
Soja
description CNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
publishDate 2017
dc.date.issued.fl_str_mv 2017
dc.date.accessioned.fl_str_mv 2022-03-28T20:34:11Z
dc.date.available.fl_str_mv 2022-03-28T20:34:11Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1843/40515
dc.identifier.doi.pt_BR.fl_str_mv 10.3920/QAS2015.0739
dc.identifier.issn.pt_BR.fl_str_mv 17578361
identifier_str_mv 10.3920/QAS2015.0739
17578361
url http://hdl.handle.net/1843/40515
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartof.pt_BR.fl_str_mv Quality Assurance and Safety of Crops & Foods
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Minas Gerais
dc.publisher.initials.fl_str_mv UFMG
dc.publisher.country.fl_str_mv Brasil
dc.publisher.department.fl_str_mv FAR - DEPARTAMENTO DE ALIMENTOS
publisher.none.fl_str_mv Universidade Federal de Minas Gerais
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFMG
instname:Universidade Federal de Minas Gerais (UFMG)
instacron:UFMG
instname_str Universidade Federal de Minas Gerais (UFMG)
instacron_str UFMG
institution UFMG
reponame_str Repositório Institucional da UFMG
collection Repositório Institucional da UFMG
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