Anti-botulism single-shot vaccine using chitosan for protein encapsulation by simple coacervation
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2016 |
| Outros Autores: | , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UFMG |
| Texto Completo: | https://doi.org/10.1016/j.anaerobe.2016.10.013 http://hdl.handle.net/1843/42502 https://orcid.org/ 0000-0001-9836-4117 https://orcid.org/0000-0001-6714-7996 |
Resumo: | Outra Agência |
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2022-06-14T14:12:39Z2022-06-14T14:12:39Z201642182187https://doi.org/10.1016/j.anaerobe.2016.10.0131075-9964http://hdl.handle.net/1843/42502https://orcid.org/ 0000-0001-9836-4117https://orcid.org/0000-0001-6714-7996Outra AgênciaThe aim of the present study was to compare the potency and safety of vaccines against Clostridium botulinum (C. botulinum) type C and D formulated with chitosan as controlled release matrix and vaccines formulated in conventional manner using aluminum hydroxide. Parameters were established for the development of chitosan microspheres, using simple coacervation to standardize the use of this polymer in protein encapsulation for vaccine formulation. To formulate a single shot vaccine inactivated antigens of C. botulinum type C and D were used with original toxin titles equal to 5.2 and 6.2 log LD50/ml, respectively. For each antigen a chitosan based solution of 50 mL was prepared. Control vaccines were formulated by mixing toxoid type C and D with aluminum hydroxide [25% Al(OH)3, pH 6.3]. The toxoid sterility, innocuity and potency of vaccines were evaluated as stipulated by MAPA-BRASIL according to ministerial directive no. 23. Encapsulation efficiency of BSA in chitosan was 32.5e40.37%, while that the encapsulation efficiency to toxoid type C was 41,03% (1.94 mg/mL) and of the toxoid type D was 32.30% (1.82 mg/mL). The single shot vaccine formulated using chitosan for protein encapsulation through simple coacervation showed potency and safety similar to conventional vaccine currently used in Brazilian livestock (10 and 2 IU/mL against C. botulinum type C and D, respectively). The present work suggests that our single shot vaccine would be a good option as a cattle vaccine against these C. botulinum type C and D.engUniversidade Federal de Minas GeraisUFMGBrasilICA - INSTITUTO DE CIÊNCIAS AGRÁRIASAnaerobeVacina veterináriaQuitosanaAntígenosAgentes nefrotóxicosAnti-botulism single-shot vaccine using chitosan for protein encapsulation by simple coacervationinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttps://www.sciencedirect.com/science/article/pii/S1075996416301329Roger Shoji SariAngelo Samir m. MigueEliane Macedo Sobrinho SantosAnna Christina de AlmeidaAlex Sander CangussuEdson Vander JorgeOtto Domenici MozzerHercules Otacilio SantosWagner QuintilioIgor Viana BrandiViviane Aguiar Andradeinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMGinstname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMGLICENSELicense.txtLicense.txttext/plain; charset=utf-82042https://repositorio.ufmg.br/bitstream/1843/42502/1/License.txtfa505098d172de0bc8864fc1287ffe22MD51ORIGINALAnti-botulism single-shot vaccine using chitosan for protein encapsulation by simple coacervation.pdfAnti-botulism single-shot vaccine using chitosan for protein encapsulation by simple coacervation.pdfapplication/pdf596958https://repositorio.ufmg.br/bitstream/1843/42502/2/Anti-botulism%20single-shot%20vaccine%20using%20chitosan%20for%20protein%20encapsulation%20by%20simple%20coacervation.pdf627f10b3e6aa79c2c0b2d406b2893bb6MD521843/425022022-06-14 11:12:39.764oai:repositorio.ufmg.br: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Repositório de PublicaçõesPUBhttps://repositorio.ufmg.br/oaiopendoar:2022-06-14T14:12:39Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)false |
| dc.title.pt_BR.fl_str_mv |
Anti-botulism single-shot vaccine using chitosan for protein encapsulation by simple coacervation |
| title |
Anti-botulism single-shot vaccine using chitosan for protein encapsulation by simple coacervation |
| spellingShingle |
Anti-botulism single-shot vaccine using chitosan for protein encapsulation by simple coacervation Roger Shoji Sari Vacina veterinária Quitosana Antígenos Agentes nefrotóxicos |
| title_short |
Anti-botulism single-shot vaccine using chitosan for protein encapsulation by simple coacervation |
| title_full |
Anti-botulism single-shot vaccine using chitosan for protein encapsulation by simple coacervation |
| title_fullStr |
Anti-botulism single-shot vaccine using chitosan for protein encapsulation by simple coacervation |
| title_full_unstemmed |
Anti-botulism single-shot vaccine using chitosan for protein encapsulation by simple coacervation |
| title_sort |
Anti-botulism single-shot vaccine using chitosan for protein encapsulation by simple coacervation |
| author |
Roger Shoji Sari |
| author_facet |
Roger Shoji Sari Angelo Samir m. Migue Eliane Macedo Sobrinho Santos Anna Christina de Almeida Alex Sander Cangussu Edson Vander Jorge Otto Domenici Mozzer Hercules Otacilio Santos Wagner Quintilio Igor Viana Brandi Viviane Aguiar Andrade |
| author_role |
author |
| author2 |
Angelo Samir m. Migue Eliane Macedo Sobrinho Santos Anna Christina de Almeida Alex Sander Cangussu Edson Vander Jorge Otto Domenici Mozzer Hercules Otacilio Santos Wagner Quintilio Igor Viana Brandi Viviane Aguiar Andrade |
| author2_role |
author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Roger Shoji Sari Angelo Samir m. Migue Eliane Macedo Sobrinho Santos Anna Christina de Almeida Alex Sander Cangussu Edson Vander Jorge Otto Domenici Mozzer Hercules Otacilio Santos Wagner Quintilio Igor Viana Brandi Viviane Aguiar Andrade |
| dc.subject.other.pt_BR.fl_str_mv |
Vacina veterinária Quitosana Antígenos Agentes nefrotóxicos |
| topic |
Vacina veterinária Quitosana Antígenos Agentes nefrotóxicos |
| description |
Outra Agência |
| publishDate |
2016 |
| dc.date.issued.fl_str_mv |
2016 |
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2022-06-14T14:12:39Z |
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2022-06-14T14:12:39Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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http://hdl.handle.net/1843/42502 |
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https://doi.org/10.1016/j.anaerobe.2016.10.013 |
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1075-9964 |
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https://orcid.org/ 0000-0001-9836-4117 https://orcid.org/0000-0001-6714-7996 |
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eng |
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Anaerobe |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Universidade Federal de Minas Gerais |
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UFMG |
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Brasil |
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ICA - INSTITUTO DE CIÊNCIAS AGRÁRIAS |
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Universidade Federal de Minas Gerais |
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