Comparative genomics of Actinobacillus pleuropneumoniae serotype 8 reveals the importance of prophages in the genetic variability of the species

• Autor(a) principal: Isabelle Gonçalves de Oliveira Prado
• Data de Publicação: 2020
• Outros Autores: Giarlã Cunha da Silva, Josicelli Souza Crispim, Pedro Marcus Pereira Vidigal, Moysés Nascimento, Mateus Ferreira Santana, Denise Mara Soares Bazzolli
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UFMG
• Texto Completo: https://doi.org/10.1155/2020/9354204; http://hdl.handle.net/1843/51300; https://orcid.org/0000-0003-4456-8303; https://orcid.org/0000-0002-8159-3009; https://orcid.org/0000-0002-1625-1088; https://orcid.org/0000-0002-5116-9856; https://orcid.org/0000-0001-5886-9540; https://orcid.org/0000-0002-9674-9408; https://orcid.org/0000-0002-0371-6966
• topic: Actinobacillus pleuropneumoniae; Genomes; Serotype; Prophages; Actinobacillus pleuropneumoniae; Genoma bacteriano; Sorogrupo; Prófagos

Actinobacillus pleuropneumoniae is the etiologic agent of porcine pleuropneumonia. Currently, there are 18 different serotypes; the serotype 8 is the most widely distributed in the United States, Canada, United Kingdom, and southeastern Brazil. In this study, genomes of seven A. pleuropneumoniae serotype 8 clinical isolates were compared to the other genomes of twelve serotypes. The analyses of serotype 8 genomes resulted in a set of 2352 protein-coding sequences. Of these sequences, 76.6% are present in all serotypes, 18.5% are shared with some serotypes, and 4.9% were differential. This differential portion was characterized as a series of hypothetical and regulatory protein sequences: mobile element sequence. Synteny analysis demonstrated possible events of gene recombination and acquisition by horizontal gene transfer (HGT) in this species. A total of 30 sequences related to prophages were identified in the genomes. These sequences represented 0.3 to 3.5% of the genome of the strains analyzed, and 16 of them contained complete prophages. Similarity analysis between complete prophage sequences evidenced a possible HGT with species belonging to the family Pasteurellaceae. Thus, mobile genetic elements, such as prophages, are important components of the differential portion of the A. pleuropneumoniae genome and demonstrate a central role in the evolution of the species. This study represents the first study done to understand the genome of A. pleuropneumoniae serotype 8.