Bone marrow mesenchymal stromal cells and dantrolene promote functional locomotor improvement in wistar rats after spinal cord injury

• Autor(a) principal: Bruno Benetti Junta Torres
• Data de Publicação: 2016
• Outros Autores: Bernardo De Caro Martins, Carla Maria Osório Silva, Mário Sérgio Lima Lavor, Sandro Coelho, Gabriela Ferreira Siano, Eliane Gonçalves de Melo
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UFMG
• Texto Completo: http://hdl.handle.net/1843/58675; https://orcid.org/0000-0002-6400-046X; https://orcid.org/0000-0003-0795-5320
• topic: Bone Marrow Mesenchymal; Stromal cell; Dantrolene; Spinal cord injury; Células-tronco Mesenquimais; Células estromais; Dantroleno; Traumatismos da medula espinal

This study aimed to evaluate the effects of bone marrow mesenchymal stromal cells (BMSC) and dantrolene (DAN) in spinal cord injury (SCI). Twenty-five male Wistar rats were divided into five groups: BMSC; BMSC+ DAN; DAN; positive control (PC) - trauma and placebo; and negative control (NC)-no trauma+placebo. Laminectomy was performed at T12 level in all animals, followed by a weight-drop model of SCI, except for the NC group. One hour later, the BMSC+DAN and DAN groups received 10mg/kg of DAN and after seven days the BMSC and BMSC+DAN groups received 1x106 BMSC intravenously. At those times, other groups received the same volume of placebo. Basso, Beattie, Bresnahan (BBB) locomotor scale was performed for 28 days to access neurological status. Traumatized animals showed severe paraplegia. There was a significant neurological improvement in groups BMSC, BMSC+ DAN and DAN from the 22th, 25thand 28thdays, respectively (p<0.05) compared to PC group. It was concluded that bone marrow mesenchymal stromal cells and dantrolene, alone or combined, for the treatment of SCI in rats promote functional neurological improvement