Conditioned Medium of Bone Marrow-Derived Mesenchymal Stromal Cells as a Therapeutic Approach to Neuropathic Pain: A Preclinical Evaluation
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2018 |
| Outros Autores: | , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| DOI: | 10.1155/2018/8179013 |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/28123 |
Resumo: | FAPESB (Grant no. DTE 0046/2011) and CNPq (Grant no. 445547/2014-6). |
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Gama, Kelly BarbosaSantos, Dourivaldo SilvaEvangelista, Afrânio FerreiraSilva, Daniela NascimentoAlcântara, Adriano Costa deSantos, Ricardo Ribeiro dosSoares, Milena Botelho PereiraVillarreal, Cristiane Flora2018-08-14T13:28:57Z2018-08-14T13:28:57Z2018GAMA, K. B. et al. Conditioned Medium of Bone Marrow-Derived Mesenchymal Stromal Cells as a Therapeutic Approach to Neuropathic Pain: A Preclinical Evaluation. Stem Cells International, art. ID 8179013, 2018.1687-966Xhttps://www.arca.fiocruz.br/handle/icict/2812310.1155/2018/8179013FAPESB (Grant no. DTE 0046/2011) and CNPq (Grant no. 445547/2014-6).Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Farmácia. Salvador, BA, BrasilUniversidade Federal da Bahia. Faculdade de Farmácia. Salvador, BA, BrasilFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, BrasilHospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, BrasilHospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasil / National Institute of Science and Technology for Regenerative Medicine. Rio de Janeiro, RJ, BrazilFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasil / National Institute of Science and Technology for Regenerative Medicine. Rio de Janeiro, RJ, BrazilFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Farmácia. Salvador, BA, BrasilNeuropathic pain is a type of chronic pain caused by injury or dysfunction of the nervous system, without effective therapeutic approaches. Mesenchymal stromal cells (MSCs), through their paracrine action, have great potential in the treatment of this syndrome. In the present study, the therapeutic potential of MSC-derived conditioned medium (CM) was investigated in a mouse model of neuropathic pain induced by partial sciatic nerve ligation (PSL). PSL mice were treated by endovenous route with bone marrow-derived MSCs (1 × 106), CM, or vehicle. Gabapentin was the reference drug. Twelve hours after administration, neuropathic mice treated with CM exhibited an antinociceptive effect that was maintained throughout the evaluation period. MSCs also induced nonreversed antinociception, while gabapentin induced short-lasting antinociception. The levels of IL-1β, TNF-α, and IL-6 were reduced, while IL-10 was enhanced on sciatic nerve and spinal cord by treatment with CM and MSCs. Preliminary analysis of the CM secretome revealed the presence of growth factors and cytokines likely involved in the antinociception. In conclusion, the CM, similar to injection of live cells, produces a powerful and long-lasting antinociceptive effect on neuropathic pain, which is related with modulatory properties on peripheral and central levels of cytokines involved with the maintenance of this syndrome.engHindawi Publishing CorporationMedula ósseaCélulas Mesenquimais EstromaisSistema nervosoAnimaisDor neuropáticaBone marrowStromal Mesenchymal CellsNervous systemAnimalsNeuropathic painConditioned Medium of Bone Marrow-Derived Mesenchymal Stromal Cells as a Therapeutic Approach to Neuropathic Pain: A Preclinical Evaluationinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/28123/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALGama KB Conditioned Medium of Bone Marrow....pdfGama KB Conditioned Medium of Bone Marrow....pdfapplication/pdf1608380https://www.arca.fiocruz.br/bitstream/icict/28123/2/Gama%20KB%20Conditioned%20Medium%20of%20Bone%20Marrow....pdf38bd383f4cde0cfd9b310fcd2fbda626MD52TEXTGama KB Conditioned Medium of Bone Marrow....pdf.txtGama KB Conditioned Medium of Bone Marrow....pdf.txtExtracted texttext/plain59368https://www.arca.fiocruz.br/bitstream/icict/28123/3/Gama%20KB%20Conditioned%20Medium%20of%20Bone%20Marrow....pdf.txt282b1eee3004c6f7c94b94c783bde1baMD53icict/281232023-03-15 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| dc.title.pt_BR.fl_str_mv |
Conditioned Medium of Bone Marrow-Derived Mesenchymal Stromal Cells as a Therapeutic Approach to Neuropathic Pain: A Preclinical Evaluation |
| title |
Conditioned Medium of Bone Marrow-Derived Mesenchymal Stromal Cells as a Therapeutic Approach to Neuropathic Pain: A Preclinical Evaluation |
| spellingShingle |
Conditioned Medium of Bone Marrow-Derived Mesenchymal Stromal Cells as a Therapeutic Approach to Neuropathic Pain: A Preclinical Evaluation Gama, Kelly Barbosa Medula óssea Células Mesenquimais Estromais Sistema nervoso Animais Dor neuropática Bone marrow Stromal Mesenchymal Cells Nervous system Animals Neuropathic pain |
| title_short |
Conditioned Medium of Bone Marrow-Derived Mesenchymal Stromal Cells as a Therapeutic Approach to Neuropathic Pain: A Preclinical Evaluation |
| title_full |
Conditioned Medium of Bone Marrow-Derived Mesenchymal Stromal Cells as a Therapeutic Approach to Neuropathic Pain: A Preclinical Evaluation |
| title_fullStr |
Conditioned Medium of Bone Marrow-Derived Mesenchymal Stromal Cells as a Therapeutic Approach to Neuropathic Pain: A Preclinical Evaluation |
| title_full_unstemmed |
Conditioned Medium of Bone Marrow-Derived Mesenchymal Stromal Cells as a Therapeutic Approach to Neuropathic Pain: A Preclinical Evaluation |
| title_sort |
Conditioned Medium of Bone Marrow-Derived Mesenchymal Stromal Cells as a Therapeutic Approach to Neuropathic Pain: A Preclinical Evaluation |
| author |
Gama, Kelly Barbosa |
| author_facet |
Gama, Kelly Barbosa Santos, Dourivaldo Silva Evangelista, Afrânio Ferreira Silva, Daniela Nascimento Alcântara, Adriano Costa de Santos, Ricardo Ribeiro dos Soares, Milena Botelho Pereira Villarreal, Cristiane Flora |
| author_role |
author |
| author2 |
Santos, Dourivaldo Silva Evangelista, Afrânio Ferreira Silva, Daniela Nascimento Alcântara, Adriano Costa de Santos, Ricardo Ribeiro dos Soares, Milena Botelho Pereira Villarreal, Cristiane Flora |
| author2_role |
author author author author author author author |
| dc.contributor.author.fl_str_mv |
Gama, Kelly Barbosa Santos, Dourivaldo Silva Evangelista, Afrânio Ferreira Silva, Daniela Nascimento Alcântara, Adriano Costa de Santos, Ricardo Ribeiro dos Soares, Milena Botelho Pereira Villarreal, Cristiane Flora |
| dc.subject.other.pt_BR.fl_str_mv |
Medula óssea Células Mesenquimais Estromais Sistema nervoso Animais Dor neuropática |
| topic |
Medula óssea Células Mesenquimais Estromais Sistema nervoso Animais Dor neuropática Bone marrow Stromal Mesenchymal Cells Nervous system Animals Neuropathic pain |
| dc.subject.en.pt_BR.fl_str_mv |
Bone marrow Stromal Mesenchymal Cells Nervous system Animals Neuropathic pain |
| description |
FAPESB (Grant no. DTE 0046/2011) and CNPq (Grant no. 445547/2014-6). |
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2018 |
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2018-08-14T13:28:57Z |
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2018-08-14T13:28:57Z |
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2018 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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GAMA, K. B. et al. Conditioned Medium of Bone Marrow-Derived Mesenchymal Stromal Cells as a Therapeutic Approach to Neuropathic Pain: A Preclinical Evaluation. Stem Cells International, art. ID 8179013, 2018. |
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https://www.arca.fiocruz.br/handle/icict/28123 |
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1687-966X |
| dc.identifier.doi.none.fl_str_mv |
10.1155/2018/8179013 |
| identifier_str_mv |
GAMA, K. B. et al. Conditioned Medium of Bone Marrow-Derived Mesenchymal Stromal Cells as a Therapeutic Approach to Neuropathic Pain: A Preclinical Evaluation. Stem Cells International, art. ID 8179013, 2018. 1687-966X 10.1155/2018/8179013 |
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Hindawi Publishing Corporation |
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Hindawi Publishing Corporation |
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