Estudo molecular do gene interferon-gamma no granuloma periapical e no cisto radicular

Detalhes bibliográficos
Autor(a) principal: Kelma Campos
Data de Publicação: 2014
Tipo de documento: Tese
Idioma: por
Título da fonte: Repositório Institucional da UFMG
Texto Completo: http://hdl.handle.net/1843/BUOS-9Q4HVL
Resumo: IFN- plays an important role in the pathogenesis of periapical lesions, and the methylation of IFNG has been associated with transcriptional inactivation. The purpose of the present study was to investigate IFNG promoter methylation in association with gene transcription and protein levels in periapical granulomas and radicular cysts. Methylation-specific (MSP) PCR was used to assess the DNA methylation pattern of the IFNG gene in 16 periapical granuloma and 13 radicular cyst samples. The transcription levels of IFNG mRNA were verified by quantitative real time PCR (qPCR), and protein expression was evaluated by immunohistochemistry. All the periapical lesion samples exhibited partial or total methylation of the IFNG gene. In addition, an increased methylation profile was found in radicular cysts compared to periapical granulomas. Increased IFNG mRNA expression was observed in the partially methylated periapical lesion samples relative to the samples that were completely methylated. The present study provides the first evidence of the possible impact of IFNG methylation on IFNG transcription in periapical lesions.
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spelling Estudo molecular do gene interferon-gamma no granuloma periapical e no cisto radicularInterferon-gammaCisto radicularLesão periapicalMetilaçãoGranuloma periapicalAbscesso periapicalCisto radicularInterferon gamaPolimorfismo genéticoMetilaçãoOdontologiaGranuloma periapicalIFN- plays an important role in the pathogenesis of periapical lesions, and the methylation of IFNG has been associated with transcriptional inactivation. The purpose of the present study was to investigate IFNG promoter methylation in association with gene transcription and protein levels in periapical granulomas and radicular cysts. Methylation-specific (MSP) PCR was used to assess the DNA methylation pattern of the IFNG gene in 16 periapical granuloma and 13 radicular cyst samples. The transcription levels of IFNG mRNA were verified by quantitative real time PCR (qPCR), and protein expression was evaluated by immunohistochemistry. All the periapical lesion samples exhibited partial or total methylation of the IFNG gene. In addition, an increased methylation profile was found in radicular cysts compared to periapical granulomas. Increased IFNG mRNA expression was observed in the partially methylated periapical lesion samples relative to the samples that were completely methylated. The present study provides the first evidence of the possible impact of IFNG methylation on IFNG transcription in periapical lesions.O IFN- apresenta importante função na patogênese das lesões periapicais e a metilação do gene IFNG tem sido associada à inativação da transcrição. O objetivo deste estudo foi investigar a metilação da região promotora do IFNG e a associação com a transcrição do gene e com os níveis de proteína no granuloma periapical e no cisto radicular. O PCR específico para metilação (MSP Methylation Specific PCR) foi usado para avaliar o padrão de metilação do DNA do gene IFNG em 16 amostras de granuloma periapical e em 13 de cisto radicular. Os níveis de transcrição do RNAm do IFNG foram verificados pelo PCR em Tempo Real (qPCR) e a expressão da proteína foi avaliada por meio da imunoistoquímica. Todas as amostras de lesão periapical exibiram metilação total ou parcial do gene IFNG. Além disso, um aumento no perfil de metilação foi encontrado nos cistos radiculares em relação aos granulomas periapicais. Observou-se um aumento na expressão do RNAm do IFNG em amostras de lesão periapical parcialmente metiladas em relação àquelas totalmente metiladas. Este estudo apresenta a primeira evidência do possível impacto da metilação do IFNG na transcrição do IFNG em lesões periapicais.Universidade Federal de Minas GeraisUFMGRicardo Santiago GomezJeane de Fatima Correia Silva AlvesVanessa de Fatima BernardesPaula Rocha MoreiraFrank Ferreira SilveiraMaria Ilma de Souza Gruppioni CortesKelma Campos2019-08-12T09:33:36Z2019-08-12T09:33:36Z2014-06-06info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisapplication/pdfhttp://hdl.handle.net/1843/BUOS-9Q4HVLinfo:eu-repo/semantics/openAccessporreponame:Repositório Institucional da UFMGinstname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMG2020-01-29T17:31:48Zoai:repositorio.ufmg.br:1843/BUOS-9Q4HVLRepositório InstitucionalPUBhttps://repositorio.ufmg.br/oairepositorio@ufmg.bropendoar:2020-01-29T17:31:48Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)false
dc.title.none.fl_str_mv Estudo molecular do gene interferon-gamma no granuloma periapical e no cisto radicular
title Estudo molecular do gene interferon-gamma no granuloma periapical e no cisto radicular
spellingShingle Estudo molecular do gene interferon-gamma no granuloma periapical e no cisto radicular
Kelma Campos
Interferon-gamma
Cisto radicular
Lesão periapical
Metilação
Granuloma periapical
Abscesso periapical
Cisto radicular
Interferon gama
Polimorfismo genético
Metilação
Odontologia
Granuloma periapical
title_short Estudo molecular do gene interferon-gamma no granuloma periapical e no cisto radicular
title_full Estudo molecular do gene interferon-gamma no granuloma periapical e no cisto radicular
title_fullStr Estudo molecular do gene interferon-gamma no granuloma periapical e no cisto radicular
title_full_unstemmed Estudo molecular do gene interferon-gamma no granuloma periapical e no cisto radicular
title_sort Estudo molecular do gene interferon-gamma no granuloma periapical e no cisto radicular
author Kelma Campos
author_facet Kelma Campos
author_role author
dc.contributor.none.fl_str_mv Ricardo Santiago Gomez
Jeane de Fatima Correia Silva Alves
Vanessa de Fatima Bernardes
Paula Rocha Moreira
Frank Ferreira Silveira
Maria Ilma de Souza Gruppioni Cortes
dc.contributor.author.fl_str_mv Kelma Campos
dc.subject.por.fl_str_mv Interferon-gamma
Cisto radicular
Lesão periapical
Metilação
Granuloma periapical
Abscesso periapical
Cisto radicular
Interferon gama
Polimorfismo genético
Metilação
Odontologia
Granuloma periapical
topic Interferon-gamma
Cisto radicular
Lesão periapical
Metilação
Granuloma periapical
Abscesso periapical
Cisto radicular
Interferon gama
Polimorfismo genético
Metilação
Odontologia
Granuloma periapical
description IFN- plays an important role in the pathogenesis of periapical lesions, and the methylation of IFNG has been associated with transcriptional inactivation. The purpose of the present study was to investigate IFNG promoter methylation in association with gene transcription and protein levels in periapical granulomas and radicular cysts. Methylation-specific (MSP) PCR was used to assess the DNA methylation pattern of the IFNG gene in 16 periapical granuloma and 13 radicular cyst samples. The transcription levels of IFNG mRNA were verified by quantitative real time PCR (qPCR), and protein expression was evaluated by immunohistochemistry. All the periapical lesion samples exhibited partial or total methylation of the IFNG gene. In addition, an increased methylation profile was found in radicular cysts compared to periapical granulomas. Increased IFNG mRNA expression was observed in the partially methylated periapical lesion samples relative to the samples that were completely methylated. The present study provides the first evidence of the possible impact of IFNG methylation on IFNG transcription in periapical lesions.
publishDate 2014
dc.date.none.fl_str_mv 2014-06-06
2019-08-12T09:33:36Z
2019-08-12T09:33:36Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1843/BUOS-9Q4HVL
url http://hdl.handle.net/1843/BUOS-9Q4HVL
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Minas Gerais
UFMG
publisher.none.fl_str_mv Universidade Federal de Minas Gerais
UFMG
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFMG
instname:Universidade Federal de Minas Gerais (UFMG)
instacron:UFMG
instname_str Universidade Federal de Minas Gerais (UFMG)
instacron_str UFMG
institution UFMG
reponame_str Repositório Institucional da UFMG
collection Repositório Institucional da UFMG
repository.name.fl_str_mv Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)
repository.mail.fl_str_mv repositorio@ufmg.br
_version_ 1823247931931623424

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