17-β-Estradiol increases macrophage activity through activation of the G-protein-coupled estrogen receptor and improves the response of female mice to Cryptococcus gattii

• Autor(a) principal: Marliete Carvalho da Costa
• Data de Publicação: 2020
• Outros Autores: Heliana de Barros Fernandes, Gleisy Kelly Neves Gonçalves, Anderson Philip Nonato Santos, Gabriella Freitas Ferreira, Gustavo José Cota de Freitas, Paulo Henrique Fonseca do Carmo, Jôsy Hubner de Sousa, Elúzia Castro Peres Emidio, Julliana Ribeiro Alves dos Santos, Adelina Martha dos Reis, Caio Tavares Fagundes, Aristóbolo Mendes da Silva, Daniel de Assis Santos
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UFMG
• Texto Completo: https://doi.org/10.1111/cmi.13179; http://hdl.handle.net/1843/51405; https://orcid.org/0000-0001-6007-9708; https://orcid.org/0000-0001-6842-1934; https://orcid.org/0000-0003-3186-885X; https://orcid.org/0000-0003-0138-9612; https://orcid.org/0000-0002-6747-5233; https://orcid.org/0000-0001-7473-7433; https://orcid.org/0000-0002-1108-5666
• topic: 17-β-estradiol; Cryptococcosis; Cryptococcus gattii; GPER; Estradiol; Criptococose; Cryptococcus gattii; Receptores acoplados a proteínas G

Cryptococcus gattii (Cg) is one of the agents of cryptococcosis, a severe systemic mycosis with a higher prevalence in men than women, but the influence of the female sex hormone, 17-β-estradiol (E2), on cryptococcosis remains unclear. Our study shows that female mice presented delayed mortality, increased neutrophil recruitment in bronchoalveolar lavage fluid, and reduced fungal load after 24 hr of infection compared to male and ovariectomised female mice (OVX). E2 replace-ment restored OVX female survival. Female macrophages have more efficient fungicidal activity, which was increased by E2 and reversed by the antagonist of G-protein-coupled oestrogen receptor (GPER), which negatively modulates PI3K activation. Furthermore, E2 induces a reduction in Cg cell diameter, cell charge, and antioxidant peroxidase activity. In conclusion, female mice present improved control of Cg infection, and GPER is important for E2 modulation of the female response.