Identification and characterization of methicillin-resistant staphylococcus spp. isolated from surfaces near patients in an intensive care unit of a hospital in southeastern brazil
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFMG |
Texto Completo: | https://doi.org/10.1590/0037-8682-0244-2020 http://hdl.handle.net/1843/42096 https://orcid.org/ 0000-0001-9836-4117 |
Resumo: | Contaminated hospital environments contribute to the transmission of microorganisms associated with healthcare. Contaminated surfaces handled by patients or healthcare professionals are a source of microorganism transmission by hand. Methicillinresistant Staphylococcus bacteria are among the main agents responsible for increasing healthcare-associated infections in Brazil and worldwide. Methods: The objective of this study was to screen and characterize methicillin-resistant Staphylococcus spp. on surfaces near patients in an intensive care unit. Microbiological samples, collected from ten beds in an intensive care unit with five sampling sites, were inoculated into a methicillin-resistant Staphylococcus aureus chromogenic medium. MALDI-TOF and PCR analyses were used to identify the bacteria. Antimicrobial susceptibility was determined using the disk diffusion test. The presence of the mecA gene was investigated using PCR. Results: We observed that 44 out of the 50 sampling sites presented grown isolates in the methicillinresistant Staphylococcus aureus medium. The incidence of isolated microorganisms on the right side rail, left side rail, tables, infusion pump keypad, and cardiac monitor were 18.8 %, 36.7 %, 10.9 %, 2.4 %, and 31 %, respectively. The 42 isolates included in this study were identified as coagulase-negative Staphylococcus. All of these microorganisms were multidrug-resistant and mecA gene-positive. Conclusions: This study identified the presence of methicillin-resistant coagulase-negative Staphylococcus on the beds of an intensive care unit, providing evidence for the necessity of assertive actions to decrease the risk of healthcare-associated infections at the site. |
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2022-05-31T10:50:30Z2022-05-31T10:50:30Z202053https://doi.org/10.1590/0037-8682-0244-20201678-9849http://hdl.handle.net/1843/42096https://orcid.org/ 0000-0001-9836-4117Contaminated hospital environments contribute to the transmission of microorganisms associated with healthcare. Contaminated surfaces handled by patients or healthcare professionals are a source of microorganism transmission by hand. Methicillinresistant Staphylococcus bacteria are among the main agents responsible for increasing healthcare-associated infections in Brazil and worldwide. Methods: The objective of this study was to screen and characterize methicillin-resistant Staphylococcus spp. on surfaces near patients in an intensive care unit. Microbiological samples, collected from ten beds in an intensive care unit with five sampling sites, were inoculated into a methicillin-resistant Staphylococcus aureus chromogenic medium. MALDI-TOF and PCR analyses were used to identify the bacteria. Antimicrobial susceptibility was determined using the disk diffusion test. The presence of the mecA gene was investigated using PCR. Results: We observed that 44 out of the 50 sampling sites presented grown isolates in the methicillinresistant Staphylococcus aureus medium. The incidence of isolated microorganisms on the right side rail, left side rail, tables, infusion pump keypad, and cardiac monitor were 18.8 %, 36.7 %, 10.9 %, 2.4 %, and 31 %, respectively. The 42 isolates included in this study were identified as coagulase-negative Staphylococcus. All of these microorganisms were multidrug-resistant and mecA gene-positive. Conclusions: This study identified the presence of methicillin-resistant coagulase-negative Staphylococcus on the beds of an intensive care unit, providing evidence for the necessity of assertive actions to decrease the risk of healthcare-associated infections at the site.engUniversidade Federal de Minas GeraisUFMGBrasilICA - INSTITUTO DE CIÊNCIAS AGRÁRIASRevista da Sociedade Brasileira de Medicina TropicalCoagulaseEquipamentos -- ContaminaçãoDrogas - Resistencia em microrganismosIdentification and characterization of methicillin-resistant staphylococcus spp. isolated from surfaces near patients in an intensive care unit of a hospital in southeastern brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttps://www.scielo.br/j/rsbmt/a/G9XpnhBSNMzKp6nLvNxztnd/?lang=enLuciano Freitas FernandesAlessandra Rejane Ericsson de Oliveira XavierGeziella Aurea Aparecida Damasceno SouzaAnna Christina de AlmeidaLéia CardosoMauro Aparecido de Sousa XavierTalles Patrick Prates PinheiroGuilherme Henrique Santos da CruzHellen Fonseca Silva DouradoWender Soares Silvainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMGinstname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMGORIGINALIDENTI~1.PDFIDENTI~1.PDFIdentification and characterization of methicillin-resistant staphylococcus spp. isolated from surfaces near patients in an intensive care unit of a hospital in southeastern Brazilapplication/pdf1466594https://repositorio.ufmg.br/bitstream/1843/42096/2/IDENTI~1.PDF57eee137113d496b6b0e0e5649b2a23eMD52LICENSELicense.txtLicense.txttext/plain; charset=utf-82042https://repositorio.ufmg.br/bitstream/1843/42096/1/License.txtfa505098d172de0bc8864fc1287ffe22MD511843/420962022-05-31 07:50:31.177oai:repositorio.ufmg.br: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Repositório de PublicaçõesPUBhttps://repositorio.ufmg.br/oaiopendoar:2022-05-31T10:50:31Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)false |
dc.title.pt_BR.fl_str_mv |
Identification and characterization of methicillin-resistant staphylococcus spp. isolated from surfaces near patients in an intensive care unit of a hospital in southeastern brazil |
title |
Identification and characterization of methicillin-resistant staphylococcus spp. isolated from surfaces near patients in an intensive care unit of a hospital in southeastern brazil |
spellingShingle |
Identification and characterization of methicillin-resistant staphylococcus spp. isolated from surfaces near patients in an intensive care unit of a hospital in southeastern brazil Luciano Freitas Fernandes Coagulase Equipamentos -- Contaminação Drogas - Resistencia em microrganismos |
title_short |
Identification and characterization of methicillin-resistant staphylococcus spp. isolated from surfaces near patients in an intensive care unit of a hospital in southeastern brazil |
title_full |
Identification and characterization of methicillin-resistant staphylococcus spp. isolated from surfaces near patients in an intensive care unit of a hospital in southeastern brazil |
title_fullStr |
Identification and characterization of methicillin-resistant staphylococcus spp. isolated from surfaces near patients in an intensive care unit of a hospital in southeastern brazil |
title_full_unstemmed |
Identification and characterization of methicillin-resistant staphylococcus spp. isolated from surfaces near patients in an intensive care unit of a hospital in southeastern brazil |
title_sort |
Identification and characterization of methicillin-resistant staphylococcus spp. isolated from surfaces near patients in an intensive care unit of a hospital in southeastern brazil |
author |
Luciano Freitas Fernandes |
author_facet |
Luciano Freitas Fernandes Alessandra Rejane Ericsson de Oliveira Xavier Geziella Aurea Aparecida Damasceno Souza Anna Christina de Almeida Léia Cardoso Mauro Aparecido de Sousa Xavier Talles Patrick Prates Pinheiro Guilherme Henrique Santos da Cruz Hellen Fonseca Silva Dourado Wender Soares Silva |
author_role |
author |
author2 |
Alessandra Rejane Ericsson de Oliveira Xavier Geziella Aurea Aparecida Damasceno Souza Anna Christina de Almeida Léia Cardoso Mauro Aparecido de Sousa Xavier Talles Patrick Prates Pinheiro Guilherme Henrique Santos da Cruz Hellen Fonseca Silva Dourado Wender Soares Silva |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Luciano Freitas Fernandes Alessandra Rejane Ericsson de Oliveira Xavier Geziella Aurea Aparecida Damasceno Souza Anna Christina de Almeida Léia Cardoso Mauro Aparecido de Sousa Xavier Talles Patrick Prates Pinheiro Guilherme Henrique Santos da Cruz Hellen Fonseca Silva Dourado Wender Soares Silva |
dc.subject.other.pt_BR.fl_str_mv |
Coagulase Equipamentos -- Contaminação Drogas - Resistencia em microrganismos |
topic |
Coagulase Equipamentos -- Contaminação Drogas - Resistencia em microrganismos |
description |
Contaminated hospital environments contribute to the transmission of microorganisms associated with healthcare. Contaminated surfaces handled by patients or healthcare professionals are a source of microorganism transmission by hand. Methicillinresistant Staphylococcus bacteria are among the main agents responsible for increasing healthcare-associated infections in Brazil and worldwide. Methods: The objective of this study was to screen and characterize methicillin-resistant Staphylococcus spp. on surfaces near patients in an intensive care unit. Microbiological samples, collected from ten beds in an intensive care unit with five sampling sites, were inoculated into a methicillin-resistant Staphylococcus aureus chromogenic medium. MALDI-TOF and PCR analyses were used to identify the bacteria. Antimicrobial susceptibility was determined using the disk diffusion test. The presence of the mecA gene was investigated using PCR. Results: We observed that 44 out of the 50 sampling sites presented grown isolates in the methicillinresistant Staphylococcus aureus medium. The incidence of isolated microorganisms on the right side rail, left side rail, tables, infusion pump keypad, and cardiac monitor were 18.8 %, 36.7 %, 10.9 %, 2.4 %, and 31 %, respectively. The 42 isolates included in this study were identified as coagulase-negative Staphylococcus. All of these microorganisms were multidrug-resistant and mecA gene-positive. Conclusions: This study identified the presence of methicillin-resistant coagulase-negative Staphylococcus on the beds of an intensive care unit, providing evidence for the necessity of assertive actions to decrease the risk of healthcare-associated infections at the site. |
publishDate |
2020 |
dc.date.issued.fl_str_mv |
2020 |
dc.date.accessioned.fl_str_mv |
2022-05-31T10:50:30Z |
dc.date.available.fl_str_mv |
2022-05-31T10:50:30Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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http://hdl.handle.net/1843/42096 |
dc.identifier.doi.pt_BR.fl_str_mv |
https://doi.org/10.1590/0037-8682-0244-2020 |
dc.identifier.issn.pt_BR.fl_str_mv |
1678-9849 |
dc.identifier.orcid.pt_BR.fl_str_mv |
https://orcid.org/ 0000-0001-9836-4117 |
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https://doi.org/10.1590/0037-8682-0244-2020 http://hdl.handle.net/1843/42096 https://orcid.org/ 0000-0001-9836-4117 |
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1678-9849 |
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eng |
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eng |
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Revista da Sociedade Brasileira de Medicina Tropical |
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Universidade Federal de Minas Gerais |
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UFMG |
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Brasil |
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ICA - INSTITUTO DE CIÊNCIAS AGRÁRIAS |
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Universidade Federal de Minas Gerais |
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