Bothrops moojeni snake peptide cyclodextrin complex: production, characterization and in vitro evaluation
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2016 |
| Outros Autores: | , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UFMG |
| Texto Completo: | https://doi.org/10.33263/LIANBS54.467474 http://hdl.handle.net/1843/61450 https://orcid.org/0000-0002-0560-8491 https://orcid.org/0000-0001-7656-1849 |
Resumo: | CAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior |
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2023-11-28T15:53:17Z2023-11-28T15:53:17Z201654467474https://doi.org/10.33263/LIANBS54.4674742284-6808http://hdl.handle.net/1843/61450https://orcid.org/0000-0002-0560-8491https://orcid.org/0000-0001-7656-1849CAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível SuperiorThe BmooMPα-I moojeni snake peptide was studied aimed to increase the therapeutic use as antitumor agent. Complexes were prepared and its physical characteristics were determined: particle size and Zeta-potential (ζ), antiproliferative and cytotoxic effects. BmooMPα-I peptide associated to Hp-βCD was able to change the cell surface parameters, such as ζ-potential which was negative. Hp-βCD:BmooMPα-I and BmooMPα-I alter the ζ of cells in contact with peptides and its association compound shown negative charge, that no favor the interaction with biological surfaces. In comparison the pure peptide as biocidal of epidermoid Carcinoma cells A431 or epithelial cells rather the fibroblast, the BmooMPα-I / Hp-βCD resulted in significant inhibition of Caco-2 human epithelial cancer cells (31.2 μg/mL after 24h), and Human Epidermoid Carcinoma cells A431(3.9 μg/mL after 24h), in a more efficient manner than peptide alone (p< 0.001), and was able to alter the degree of hemolysis. Next, the Lactate Dehydrogenase was examined to know the peptide cytotoxic effects and showed lower cytotoxic effects on EC50 for fibroblastic cells. Based on the BmooMPα-I /Hp-βCD properties observed and its ability of inhibiting the proliferation of epithelial cells, this study highlight that this compound is a biocidal with high potential as an anti-tumoral agent.engUniversidade Federal de Minas GeraisUFMGBrasilFAO - DEPARTAMENTO DE ODONTOLOGIA RESTAURADORAICB - DEPARTAMENTO DE FISIOLOGIA E BIOFÍSICAICX - DEPARTAMENTO DE QUÍMICALetters in Applied NanoBioScienceCiclodextrinasCarcinoma de células escamosasAgentes antineoplásicosPotencial zetaSistemas de distribuição de medicamentosBmooMPα-IPeptideHydroxypropyl-beta-cyclodextrinHuman epidermoid carcinoma cellsSlow deliveryBothrops moojeni snake peptide cyclodextrin complex: production, characterization and in vitro evaluationinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttps://nanobioletters.com/wp-content/uploads/2021/02/2284680853467474.pdfKarina Imaculada Rosa TeixeiraRobson Augusto de Souza SantosFabio de OliveiraRubén Dario Sinisterra MillánMaría Esperanza Cortés Seguraapplication/pdfinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMGinstname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMGLICENSELicense.txtLicense.txttext/plain; charset=utf-82042https://repositorio.ufmg.br/bitstream/1843/61450/1/License.txtfa505098d172de0bc8864fc1287ffe22MD51ORIGINALBothrops moojeni snake peptide cyclodextrin complex.pdfBothrops moojeni snake peptide cyclodextrin complex.pdfapplication/pdf741219https://repositorio.ufmg.br/bitstream/1843/61450/2/Bothrops%20moojeni%20snake%20peptide%20cyclodextrin%20complex.pdfda4561d40e488c4bea019968b0cc0a15MD521843/614502023-11-28 12:53:18.149oai:repositorio.ufmg.br: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Repositório de PublicaçõesPUBhttps://repositorio.ufmg.br/oaiopendoar:2023-11-28T15:53:18Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)false |
| dc.title.pt_BR.fl_str_mv |
Bothrops moojeni snake peptide cyclodextrin complex: production, characterization and in vitro evaluation |
| title |
Bothrops moojeni snake peptide cyclodextrin complex: production, characterization and in vitro evaluation |
| spellingShingle |
Bothrops moojeni snake peptide cyclodextrin complex: production, characterization and in vitro evaluation Karina Imaculada Rosa Teixeira BmooMPα-I Peptide Hydroxypropyl-beta-cyclodextrin Human epidermoid carcinoma cells Slow delivery Ciclodextrinas Carcinoma de células escamosas Agentes antineoplásicos Potencial zeta Sistemas de distribuição de medicamentos |
| title_short |
Bothrops moojeni snake peptide cyclodextrin complex: production, characterization and in vitro evaluation |
| title_full |
Bothrops moojeni snake peptide cyclodextrin complex: production, characterization and in vitro evaluation |
| title_fullStr |
Bothrops moojeni snake peptide cyclodextrin complex: production, characterization and in vitro evaluation |
| title_full_unstemmed |
Bothrops moojeni snake peptide cyclodextrin complex: production, characterization and in vitro evaluation |
| title_sort |
Bothrops moojeni snake peptide cyclodextrin complex: production, characterization and in vitro evaluation |
| author |
Karina Imaculada Rosa Teixeira |
| author_facet |
Karina Imaculada Rosa Teixeira Robson Augusto de Souza Santos Fabio de Oliveira Rubén Dario Sinisterra Millán María Esperanza Cortés Segura |
| author_role |
author |
| author2 |
Robson Augusto de Souza Santos Fabio de Oliveira Rubén Dario Sinisterra Millán María Esperanza Cortés Segura |
| author2_role |
author author author author |
| dc.contributor.author.fl_str_mv |
Karina Imaculada Rosa Teixeira Robson Augusto de Souza Santos Fabio de Oliveira Rubén Dario Sinisterra Millán María Esperanza Cortés Segura |
| dc.subject.por.fl_str_mv |
BmooMPα-I Peptide Hydroxypropyl-beta-cyclodextrin Human epidermoid carcinoma cells Slow delivery |
| topic |
BmooMPα-I Peptide Hydroxypropyl-beta-cyclodextrin Human epidermoid carcinoma cells Slow delivery Ciclodextrinas Carcinoma de células escamosas Agentes antineoplásicos Potencial zeta Sistemas de distribuição de medicamentos |
| dc.subject.other.pt_BR.fl_str_mv |
Ciclodextrinas Carcinoma de células escamosas Agentes antineoplásicos Potencial zeta Sistemas de distribuição de medicamentos |
| description |
CAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior |
| publishDate |
2016 |
| dc.date.issued.fl_str_mv |
2016 |
| dc.date.accessioned.fl_str_mv |
2023-11-28T15:53:17Z |
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2023-11-28T15:53:17Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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http://hdl.handle.net/1843/61450 |
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https://doi.org/10.33263/LIANBS54.467474 |
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2284-6808 |
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https://orcid.org/0000-0002-0560-8491 https://orcid.org/0000-0001-7656-1849 |
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https://doi.org/10.33263/LIANBS54.467474 http://hdl.handle.net/1843/61450 https://orcid.org/0000-0002-0560-8491 https://orcid.org/0000-0001-7656-1849 |
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2284-6808 |
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eng |
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eng |
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Letters in Applied NanoBioScience |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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Universidade Federal de Minas Gerais |
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UFMG |
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Brasil |
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FAO - DEPARTAMENTO DE ODONTOLOGIA RESTAURADORA ICB - DEPARTAMENTO DE FISIOLOGIA E BIOFÍSICA ICX - DEPARTAMENTO DE QUÍMICA |
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Universidade Federal de Minas Gerais |
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reponame:Repositório Institucional da UFMG instname:Universidade Federal de Minas Gerais (UFMG) instacron:UFMG |
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