Toxicological effect of essential oils of plants against Artemia salina
Autor(a) principal: | |
---|---|
Data de Publicação: | 2022 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFMG |
Texto Completo: | http://hdl.handle.net/1843/59717 |
Resumo: | The present study was carried out with the objective of assessing the acute toxicity of four essential plant oils against the brine shrimp lethality bioassay. The essential oils were dissolved in dimethyl sulfoxide (DMSO) at 1% and concentrations at 0.01; 0.05; 0.25; 1.25; 6.25 and 31.25 µg/ml were prepared which were added in microcells of 96 dishes containing from 10 to 15 nauplii of Artemia salina in saline solution with a total volume of 150 µL. Each concentration was replicated 8 times. Two controls were used, one with saline water solution containing 38 g of coarse salt/l and the other with DMSO 1 % solvent dissolved in the saline solution. Samples were maintained with light and at environmental temperature. Larvae death on exposing them to contact for 24 h was counted. The Lethal Concentration 50 (LC50) was determined by the Probit method using the statistical package SPSS, version 22. All essential oils tested manifested a high acute toxicity at low dosages. These showed high lethality and its results followed by order of toxicity were: Cymbopogon citratus (LC50= 1.212 µg/ml) >Lippia rotundifolia (LC50= 1.256 µg/ml) >Lippia origanoides (LC50= 1.267 µg/ml) >Cymbopogon citratus Lemon-Grass© (LC50= 1.284 µg/ml). It is concluded that given the lethality exhibited by the essential oils of Cympogogon citratus Lemon-Grass©, C.citratus, Lippia origanoides and Lippia rotundifolia against the Artemia salina micro-crustaceous can be considered promissory essential oils for its use for the control of harmful organisms. Other more specific studies are recommended for demonstrating its biological activity. |
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2023-10-19T16:11:29Z2023-10-19T16:11:29Z2022-01-25212612681983-084Xhttp://hdl.handle.net/1843/59717The present study was carried out with the objective of assessing the acute toxicity of four essential plant oils against the brine shrimp lethality bioassay. The essential oils were dissolved in dimethyl sulfoxide (DMSO) at 1% and concentrations at 0.01; 0.05; 0.25; 1.25; 6.25 and 31.25 µg/ml were prepared which were added in microcells of 96 dishes containing from 10 to 15 nauplii of Artemia salina in saline solution with a total volume of 150 µL. Each concentration was replicated 8 times. Two controls were used, one with saline water solution containing 38 g of coarse salt/l and the other with DMSO 1 % solvent dissolved in the saline solution. Samples were maintained with light and at environmental temperature. Larvae death on exposing them to contact for 24 h was counted. The Lethal Concentration 50 (LC50) was determined by the Probit method using the statistical package SPSS, version 22. All essential oils tested manifested a high acute toxicity at low dosages. These showed high lethality and its results followed by order of toxicity were: Cymbopogon citratus (LC50= 1.212 µg/ml) >Lippia rotundifolia (LC50= 1.256 µg/ml) >Lippia origanoides (LC50= 1.267 µg/ml) >Cymbopogon citratus Lemon-Grass© (LC50= 1.284 µg/ml). It is concluded that given the lethality exhibited by the essential oils of Cympogogon citratus Lemon-Grass©, C.citratus, Lippia origanoides and Lippia rotundifolia against the Artemia salina micro-crustaceous can be considered promissory essential oils for its use for the control of harmful organisms. Other more specific studies are recommended for demonstrating its biological activity.O presente estudo foi realizado com o objetivo de avaliar a toxicidade aguda de óleos essenciais frente à Artemia salina. Os óleos essenciais foram dissolvidos em dimetilsulfóxido (DMSO) a 1% e em concentrações de 0,01; 0,05; 0,25; 1,25; 6,25 e 31,25 µg/ml e foram adicionados em microplacas de 96 poços contendo de 10 a 15 náuplios de Artemia salina em solução salina totalizando um volume de 150 µl, sendo que o ensaio com cada concentração foi repetido 8 vezes. Os controles foram com solução salina (38 g/l) e o outro com DMSO (1%). As amostras foram mantidas com luz e à temperatura ambiente e a mortalidade das larvas avaliada após 24h. A concentração letal 50 (CL50) foi determinada pelo método Probit utilizando o pacote estatístico SPSS, versão 22. Todos os óleos essenciais testados manifestaram uma toxicidade aguda elevada a baixas dosagens. Os óleos que apresentaram alta letalidade foram o Cymbopogon citratus (CL50= 1.212 µg/ml) >Lippia rotundifolia (CL50= 1.256 µg/ml) >Lippia origanoides (CL50=1.267 µg/ml) >Cymbopogon citratus Lemon-Grass© (CL50= 1.284 µg/ml). Conclui-se que, dada a letalidade exibida pelos óleos essenciais de Cympogogon citratus Lemon-Grass©, C. citratus, Lippia origanoides e Lippia rotundifolia frente aos microcrustáceos, Artemia salina, esses óleos essenciais podem ser considerados eficientes para serem utilizados no controlo de organismos nocivos. Estudos mais específicos são recomendados para demonstrar a sua atividade biológica.CNPq - Conselho Nacional de Desenvolvimento Científico e TecnológicoCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível SuperiorOutra AgênciaengUniversidade Federal de Minas GeraisUFMGBrasilICA - INSTITUTO DE CIÊNCIAS AGRÁRIASRevista Brasileira de Plantas MedicinaisPlantas medicinaisEssências e óleos essenciaisToxicologiaplantas medicinaisóleos essenciaisToxicological effect of essential oils of plants against Artemia salinaEfeito toxicológico de óleos essenciais das plantas contra Artemia salinainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://sbpmed.org.br/admin/files/papers/file_xjvnq3oIE5Y6.pdfNurys Valenciaga ValdésErnane Ronie MartinsFrancine Souza Alves da Fonsecainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMGinstname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMGLICENSELicense.txtLicense.txttext/plain; charset=utf-82042https://repositorio.ufmg.br/bitstream/1843/59717/1/License.txtfa505098d172de0bc8864fc1287ffe22MD51ORIGINALToxicological effect of essential oils of plants against Artemia salina.pdfToxicological effect of essential oils of plants against Artemia salina.pdfapplication/pdf526622https://repositorio.ufmg.br/bitstream/1843/59717/2/Toxicological%20effect%20of%20essential%20oils%20of%20plants%20against%20Artemia%20salina.pdf40643395fffc97918213411a108291d9MD521843/597172023-10-19 16:26:52.285oai:repositorio.ufmg.br:1843/59717TElDRU7vv71BIERFIERJU1RSSUJVSe+/ve+/vU8gTu+/vU8tRVhDTFVTSVZBIERPIFJFUE9TSVTvv71SSU8gSU5TVElUVUNJT05BTCBEQSBVRk1HCiAKCkNvbSBhIGFwcmVzZW50Ye+/ve+/vW8gZGVzdGEgbGljZW7vv71hLCB2b2Pvv70gKG8gYXV0b3IgKGVzKSBvdSBvIHRpdHVsYXIgZG9zIGRpcmVpdG9zIGRlIGF1dG9yKSBjb25jZWRlIGFvIFJlcG9zaXTvv71yaW8gSW5zdGl0dWNpb25hbCBkYSBVRk1HIChSSS1VRk1HKSBvIGRpcmVpdG8gbu+/vW8gZXhjbHVzaXZvIGUgaXJyZXZvZ++/vXZlbCBkZSByZXByb2R1emlyIGUvb3UgZGlzdHJpYnVpciBhIHN1YSBwdWJsaWNh77+977+9byAoaW5jbHVpbmRvIG8gcmVzdW1vKSBwb3IgdG9kbyBvIG11bmRvIG5vIGZvcm1hdG8gaW1wcmVzc28gZSBlbGV0cu+/vW5pY28gZSBlbSBxdWFscXVlciBtZWlvLCBpbmNsdWluZG8gb3MgZm9ybWF0b3Mg77+9dWRpbyBvdSB277+9ZGVvLgoKVm9j77+9IGRlY2xhcmEgcXVlIGNvbmhlY2UgYSBwb2zvv710aWNhIGRlIGNvcHlyaWdodCBkYSBlZGl0b3JhIGRvIHNldSBkb2N1bWVudG8gZSBxdWUgY29uaGVjZSBlIGFjZWl0YSBhcyBEaXJldHJpemVzIGRvIFJJLVVGTUcuCgpWb2Pvv70gY29uY29yZGEgcXVlIG8gUmVwb3NpdO+/vXJpbyBJbnN0aXR1Y2lvbmFsIGRhIFVGTUcgcG9kZSwgc2VtIGFsdGVyYXIgbyBjb250Ze+/vWRvLCB0cmFuc3BvciBhIHN1YSBwdWJsaWNh77+977+9byBwYXJhIHF1YWxxdWVyIG1laW8gb3UgZm9ybWF0byBwYXJhIGZpbnMgZGUgcHJlc2VydmHvv73vv71vLgoKVm9j77+9IHRhbWLvv71tIGNvbmNvcmRhIHF1ZSBvIFJlcG9zaXTvv71yaW8gSW5zdGl0dWNpb25hbCBkYSBVRk1HIHBvZGUgbWFudGVyIG1haXMgZGUgdW1hIGPvv71waWEgZGUgc3VhIHB1YmxpY2Hvv73vv71vIHBhcmEgZmlucyBkZSBzZWd1cmFu77+9YSwgYmFjay11cCBlIHByZXNlcnZh77+977+9by4KClZvY++/vSBkZWNsYXJhIHF1ZSBhIHN1YSBwdWJsaWNh77+977+9byDvv70gb3JpZ2luYWwgZSBxdWUgdm9j77+9IHRlbSBvIHBvZGVyIGRlIGNvbmNlZGVyIG9zIGRpcmVpdG9zIGNvbnRpZG9zIG5lc3RhIGxpY2Vu77+9YS4gVm9j77+9IHRhbWLvv71tIGRlY2xhcmEgcXVlIG8gZGVw77+9c2l0byBkZSBzdWEgcHVibGljYe+/ve+/vW8gbu+/vW8sIHF1ZSBzZWphIGRlIHNldSBjb25oZWNpbWVudG8sIGluZnJpbmdlIGRpcmVpdG9zIGF1dG9yYWlzIGRlIG5pbmd177+9bS4KCkNhc28gYSBzdWEgcHVibGljYe+/ve+/vW8gY29udGVuaGEgbWF0ZXJpYWwgcXVlIHZvY++/vSBu77+9byBwb3NzdWkgYSB0aXR1bGFyaWRhZGUgZG9zIGRpcmVpdG9zIGF1dG9yYWlzLCB2b2Pvv70gZGVjbGFyYSBxdWUgb2J0ZXZlIGEgcGVybWlzc++/vW8gaXJyZXN0cml0YSBkbyBkZXRlbnRvciBkb3MgZGlyZWl0b3MgYXV0b3JhaXMgcGFyYSBjb25jZWRlciBhbyBSZXBvc2l077+9cmlvIEluc3RpdHVjaW9uYWwgZGEgVUZNRyBvcyBkaXJlaXRvcyBhcHJlc2VudGFkb3MgbmVzdGEgbGljZW7vv71hLCBlIHF1ZSBlc3NlIG1hdGVyaWFsIGRlIHByb3ByaWVkYWRlIGRlIHRlcmNlaXJvcyBlc3Tvv70gY2xhcmFtZW50ZSBpZGVudGlmaWNhZG8gZSByZWNvbmhlY2lkbyBubyB0ZXh0byBvdSBubyBjb250Ze+/vWRvIGRhIHB1YmxpY2Hvv73vv71vIG9yYSBkZXBvc2l0YWRhLgoKQ0FTTyBBIFBVQkxJQ0Hvv73vv71PIE9SQSBERVBPU0lUQURBIFRFTkhBIFNJRE8gUkVTVUxUQURPIERFIFVNIFBBVFJPQ++/vU5JTyBPVSBBUE9JTyBERSBVTUEgQUfvv71OQ0lBIERFIEZPTUVOVE8gT1UgT1VUUk8gT1JHQU5JU01PLCBWT0Pvv70gREVDTEFSQSBRVUUgUkVTUEVJVE9VIFRPRE9TIEUgUVVBSVNRVUVSIERJUkVJVE9TIERFIFJFVklT77+9TyBDT01PIFRBTULvv71NIEFTIERFTUFJUyBPQlJJR0Hvv73vv71FUyBFWElHSURBUyBQT1IgQ09OVFJBVE8gT1UgQUNPUkRPLgoKTyBSZXBvc2l077+9cmlvIEluc3RpdHVjaW9uYWwgZGEgVUZNRyBzZSBjb21wcm9tZXRlIGEgaWRlbnRpZmljYXIgY2xhcmFtZW50ZSBvIHNldSBub21lKHMpIG91IG8ocykgbm9tZXMocykgZG8ocykgZGV0ZW50b3IoZXMpIGRvcyBkaXJlaXRvcyBhdXRvcmFpcyBkYSBwdWJsaWNh77+977+9bywgZSBu77+9byBmYXLvv70gcXVhbHF1ZXIgYWx0ZXJh77+977+9bywgYWzvv71tIGRhcXVlbGFzIGNvbmNlZGlkYXMgcG9yIGVzdGEgbGljZW7vv71hLgo=Repositório de PublicaçõesPUBhttps://repositorio.ufmg.br/oaiopendoar:2023-10-19T19:26:52Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)false |
dc.title.pt_BR.fl_str_mv |
Toxicological effect of essential oils of plants against Artemia salina |
dc.title.alternative.pt_BR.fl_str_mv |
Efeito toxicológico de óleos essenciais das plantas contra Artemia salina |
title |
Toxicological effect of essential oils of plants against Artemia salina |
spellingShingle |
Toxicological effect of essential oils of plants against Artemia salina Nurys Valenciaga Valdés plantas medicinais óleos essenciais Plantas medicinais Essências e óleos essenciais Toxicologia |
title_short |
Toxicological effect of essential oils of plants against Artemia salina |
title_full |
Toxicological effect of essential oils of plants against Artemia salina |
title_fullStr |
Toxicological effect of essential oils of plants against Artemia salina |
title_full_unstemmed |
Toxicological effect of essential oils of plants against Artemia salina |
title_sort |
Toxicological effect of essential oils of plants against Artemia salina |
author |
Nurys Valenciaga Valdés |
author_facet |
Nurys Valenciaga Valdés Ernane Ronie Martins Francine Souza Alves da Fonseca |
author_role |
author |
author2 |
Ernane Ronie Martins Francine Souza Alves da Fonseca |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Nurys Valenciaga Valdés Ernane Ronie Martins Francine Souza Alves da Fonseca |
dc.subject.por.fl_str_mv |
plantas medicinais óleos essenciais |
topic |
plantas medicinais óleos essenciais Plantas medicinais Essências e óleos essenciais Toxicologia |
dc.subject.other.pt_BR.fl_str_mv |
Plantas medicinais Essências e óleos essenciais Toxicologia |
description |
The present study was carried out with the objective of assessing the acute toxicity of four essential plant oils against the brine shrimp lethality bioassay. The essential oils were dissolved in dimethyl sulfoxide (DMSO) at 1% and concentrations at 0.01; 0.05; 0.25; 1.25; 6.25 and 31.25 µg/ml were prepared which were added in microcells of 96 dishes containing from 10 to 15 nauplii of Artemia salina in saline solution with a total volume of 150 µL. Each concentration was replicated 8 times. Two controls were used, one with saline water solution containing 38 g of coarse salt/l and the other with DMSO 1 % solvent dissolved in the saline solution. Samples were maintained with light and at environmental temperature. Larvae death on exposing them to contact for 24 h was counted. The Lethal Concentration 50 (LC50) was determined by the Probit method using the statistical package SPSS, version 22. All essential oils tested manifested a high acute toxicity at low dosages. These showed high lethality and its results followed by order of toxicity were: Cymbopogon citratus (LC50= 1.212 µg/ml) >Lippia rotundifolia (LC50= 1.256 µg/ml) >Lippia origanoides (LC50= 1.267 µg/ml) >Cymbopogon citratus Lemon-Grass© (LC50= 1.284 µg/ml). It is concluded that given the lethality exhibited by the essential oils of Cympogogon citratus Lemon-Grass©, C.citratus, Lippia origanoides and Lippia rotundifolia against the Artemia salina micro-crustaceous can be considered promissory essential oils for its use for the control of harmful organisms. Other more specific studies are recommended for demonstrating its biological activity. |
publishDate |
2022 |
dc.date.issued.fl_str_mv |
2022-01-25 |
dc.date.accessioned.fl_str_mv |
2023-10-19T16:11:29Z |
dc.date.available.fl_str_mv |
2023-10-19T16:11:29Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1843/59717 |
dc.identifier.issn.pt_BR.fl_str_mv |
1983-084X |
identifier_str_mv |
1983-084X |
url |
http://hdl.handle.net/1843/59717 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.none.fl_str_mv |
Revista Brasileira de Plantas Medicinais |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Universidade Federal de Minas Gerais |
dc.publisher.initials.fl_str_mv |
UFMG |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
ICA - INSTITUTO DE CIÊNCIAS AGRÁRIAS |
publisher.none.fl_str_mv |
Universidade Federal de Minas Gerais |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFMG instname:Universidade Federal de Minas Gerais (UFMG) instacron:UFMG |
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Universidade Federal de Minas Gerais (UFMG) |
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UFMG |
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UFMG |
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Repositório Institucional da UFMG |
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