Essential oils and benzylaminopurine (BAP) for multiplication in vitro of Dimorphandra mollis

Detalhes bibliográficos
Autor(a) principal: Molinari, Letícia Vaz
Data de Publicação: 2021
Outros Autores: Martins, Karoline Ferreira, Nunes, Claudinéia Ferreira, Martins, Ernane Ronie, Meira, Messulan Rodrigues
Tipo de documento: Artigo
Idioma: por
Título da fonte: Pesquisa Florestal Brasileira (Online)
Texto Completo: https://pfb.cnpf.embrapa.br/pfb/index.php/pfb/article/view/1926
Resumo: Micropropagation is an alternative for genetic conservation of Cerrado endemic species, such as Dimorphandra mollis (fava-d’anta), making possible maximizing propagation in high quality. To solve the limiting factors of in vitro cultivation of D. mollis, the present study aimed to evaluate the efficiency of essential oils as a sanitizer and to test concentrations of benzylaminopurine growth regulator (BAP) in the in vitro multiplication phase. Seeds collected from a natural population were scarified and disinfected with sodium hypochlorite and essential oils of Lippia origanoides, Ocimum gratissimum, L. rotundifolia and Cymbopogon citratus. The seeds were inoculated in MS culture medium. In vitro multiplication was evaluated using four concentrations of BAP (0.5; 1.0; 1.5 and 2.0 mg L-1). Essential oil of C. citratus is the most suitable for disinfestation of fava d’anta seeds, in the stage of establishment in vitro. Phytoregulators used in the present study did not provide satisfactory results to define an in vitro multiplication protocol for the species.
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spelling Essential oils and benzylaminopurine (BAP) for multiplication in vitro of Dimorphandra mollisÓleos essenciais e benzilaminopurina (BAP) para o cultivo in vitro de Dimorphandra mollisPlantas medicinais. Cultivo in vitro. Óleos essenciais.Micropropagation is an alternative for genetic conservation of Cerrado endemic species, such as Dimorphandra mollis (fava-d’anta), making possible maximizing propagation in high quality. To solve the limiting factors of in vitro cultivation of D. mollis, the present study aimed to evaluate the efficiency of essential oils as a sanitizer and to test concentrations of benzylaminopurine growth regulator (BAP) in the in vitro multiplication phase. Seeds collected from a natural population were scarified and disinfected with sodium hypochlorite and essential oils of Lippia origanoides, Ocimum gratissimum, L. rotundifolia and Cymbopogon citratus. The seeds were inoculated in MS culture medium. In vitro multiplication was evaluated using four concentrations of BAP (0.5; 1.0; 1.5 and 2.0 mg L-1). Essential oil of C. citratus is the most suitable for disinfestation of fava d’anta seeds, in the stage of establishment in vitro. Phytoregulators used in the present study did not provide satisfactory results to define an in vitro multiplication protocol for the species.A micropropagação é uma alternativa para a conservação genética de espécies endêmicas do Cerrado, como Dimorphandra mollis (fava-d’anta), possibilitando a maximização da propagação em elevada qualidade. Para solucionar os fatores limitantes ao cultivo in vitro de D. mollis, o presente trabalho teve como objetivo avaliar a eficiência de óleos essenciais como sanitizante e testar concentrações de regulador de crescimento benzilaminopurina (BAP) na fase de multiplicação in vitro. Sementes coletadas em população natural foram escarificadas e desinfestadas com hipoclorito de sódio e óleos essenciais de Lippia origanoides, Ocimum gratissimum, L. rotundifolia e Cymbopogon citratus, sendo posteriormente inoculadas em meio de cultura MS. A multiplicação in vitro foi avaliada em quatro concentrações de BAP (0,5; 1,0; 1,5 e 2,0 mg L-1). O óleo essencial de C. citratus se mostrou o mais adequado para a desinfestação de sementes da fava d’anta, na fase de estabelecimento in vitro. Fitoreguladores utilizados no presente estudo não promoveram resultados satisfatórios para definir um protocolo de multiplicação in vitro para a espécie.Embrapa Florestas2021-09-14info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://pfb.cnpf.embrapa.br/pfb/index.php/pfb/article/view/192610.4336/2021.pfb.41e201901926Pesquisa Florestal Brasileira; v. 41 (2021)Pesquisa Florestal Brasileira; Vol. 41 (2021)1983-26051809-3647reponame:Pesquisa Florestal Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAporhttps://pfb.cnpf.embrapa.br/pfb/index.php/pfb/article/view/1926/1642https://creativecommons.org/licenses/by-nc-nd/4.0info:eu-repo/semantics/openAccessMolinari, Letícia VazMartins, Karoline FerreiraNunes, Claudinéia FerreiraMartins, Ernane RonieMeira, Messulan Rodrigues2021-11-18T11:01:58Zoai:pfb.cnpf.embrapa.br/pfb:article/1926Revistahttps://pfb.cnpf.embrapa.br/pfb/index.php/pfb/PUBhttps://pfb.cnpf.embrapa.br/pfb/index.php/pfb/oaipfb@embrapa.br || revista.pfb@gmail.com || patricia.mattos@embrapa.br1983-26051809-3647opendoar:2021-11-18T11:01:58Pesquisa Florestal Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Essential oils and benzylaminopurine (BAP) for multiplication in vitro of Dimorphandra mollis
Óleos essenciais e benzilaminopurina (BAP) para o cultivo in vitro de Dimorphandra mollis
title Essential oils and benzylaminopurine (BAP) for multiplication in vitro of Dimorphandra mollis
spellingShingle Essential oils and benzylaminopurine (BAP) for multiplication in vitro of Dimorphandra mollis
Molinari, Letícia Vaz
Plantas medicinais. Cultivo in vitro. Óleos essenciais.
title_short Essential oils and benzylaminopurine (BAP) for multiplication in vitro of Dimorphandra mollis
title_full Essential oils and benzylaminopurine (BAP) for multiplication in vitro of Dimorphandra mollis
title_fullStr Essential oils and benzylaminopurine (BAP) for multiplication in vitro of Dimorphandra mollis
title_full_unstemmed Essential oils and benzylaminopurine (BAP) for multiplication in vitro of Dimorphandra mollis
title_sort Essential oils and benzylaminopurine (BAP) for multiplication in vitro of Dimorphandra mollis
author Molinari, Letícia Vaz
author_facet Molinari, Letícia Vaz
Martins, Karoline Ferreira
Nunes, Claudinéia Ferreira
Martins, Ernane Ronie
Meira, Messulan Rodrigues
author_role author
author2 Martins, Karoline Ferreira
Nunes, Claudinéia Ferreira
Martins, Ernane Ronie
Meira, Messulan Rodrigues
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Molinari, Letícia Vaz
Martins, Karoline Ferreira
Nunes, Claudinéia Ferreira
Martins, Ernane Ronie
Meira, Messulan Rodrigues
dc.subject.por.fl_str_mv Plantas medicinais. Cultivo in vitro. Óleos essenciais.
topic Plantas medicinais. Cultivo in vitro. Óleos essenciais.
description Micropropagation is an alternative for genetic conservation of Cerrado endemic species, such as Dimorphandra mollis (fava-d’anta), making possible maximizing propagation in high quality. To solve the limiting factors of in vitro cultivation of D. mollis, the present study aimed to evaluate the efficiency of essential oils as a sanitizer and to test concentrations of benzylaminopurine growth regulator (BAP) in the in vitro multiplication phase. Seeds collected from a natural population were scarified and disinfected with sodium hypochlorite and essential oils of Lippia origanoides, Ocimum gratissimum, L. rotundifolia and Cymbopogon citratus. The seeds were inoculated in MS culture medium. In vitro multiplication was evaluated using four concentrations of BAP (0.5; 1.0; 1.5 and 2.0 mg L-1). Essential oil of C. citratus is the most suitable for disinfestation of fava d’anta seeds, in the stage of establishment in vitro. Phytoregulators used in the present study did not provide satisfactory results to define an in vitro multiplication protocol for the species.
publishDate 2021
dc.date.none.fl_str_mv 2021-09-14
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
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dc.identifier.uri.fl_str_mv https://pfb.cnpf.embrapa.br/pfb/index.php/pfb/article/view/1926
10.4336/2021.pfb.41e201901926
url https://pfb.cnpf.embrapa.br/pfb/index.php/pfb/article/view/1926
identifier_str_mv 10.4336/2021.pfb.41e201901926
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://pfb.cnpf.embrapa.br/pfb/index.php/pfb/article/view/1926/1642
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by-nc-nd/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Embrapa Florestas
publisher.none.fl_str_mv Embrapa Florestas
dc.source.none.fl_str_mv Pesquisa Florestal Brasileira; v. 41 (2021)
Pesquisa Florestal Brasileira; Vol. 41 (2021)
1983-2605
1809-3647
reponame:Pesquisa Florestal Brasileira (Online)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Florestal Brasileira (Online)
collection Pesquisa Florestal Brasileira (Online)
repository.name.fl_str_mv Pesquisa Florestal Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv pfb@embrapa.br || revista.pfb@gmail.com || patricia.mattos@embrapa.br
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