Total combining power: technique for the evaluation of the quality control process of clostridiosis vaccines
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2016 |
| Outros Autores: | , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UFMG |
| Texto Completo: | https://doi.org/10.1016/j.mimet.2016.08.023 http://hdl.handle.net/1843/42608 https://orcid.org/0000-0001-6714-7996 https://orcid.org/ 0000-0001-8980-8599 |
Resumo: | Outra Agência |
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2022-06-23T11:14:04Z2022-06-23T11:14:04Z2016130164168https://doi.org/10.1016/j.mimet.2016.08.0230167-7012http://hdl.handle.net/1843/42608https://orcid.org/0000-0001-6714-7996https://orcid.org/ 0000-0001-8980-8599Outra AgênciaAn efficient technique for evaluation of the quality control of vaccines against clostridiosis is described in this study. This technique is capable of quantifying the toxoid of the bacterium Clostridium perfringens Type D, which is commonly found within these vaccines. The described method is performed in vivo to quantify the toxoid, replacing the current predominant approaches that use the titration of toxins before the inactivation process. This method is based on the partial neutralization of a determined dose of antitoxin by testing different doses of the toxoid. In order to guarantee its reliability, it is essential for the technique to be validated. Thus, the technique was tested using the following validation parameters: specificity and selectivity, detection limit, linear correlation, precision and robustness, in agreement with the requirements of regulatory agencies and international committees from around the world. The method was found to be specific, selective, robust, precise, and linear inside a specific concentration range. Therefore, it could be applied to the quality control of clostridiosis vaccines with satisfactory results.engUniversidade Federal de Minas GeraisUFMGBrasilICA - INSTITUTO DE CIÊNCIAS AGRÁRIASJournal of Microbiological MethodsVacina veterináriaClostridiosesToxinas bacterianasTotal combining power: technique for the evaluation of the quality control process of clostridiosis vaccinesPoder total de combinação: Técnica para avaliação do processo de controle de qualidade de vacinas contra clostridioseinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttps://www.sciencedirect.com/science/article/pii/S0167701216302330Igor Viana BrandiEliane Macedo Sobrinho SantosBruna Mara Aparecida de Carvalho MesquitaCarla Adriana Ferreira DurãesPaula Karoline Soares FariasRoger Shoji SariAlex Sander Rodrigues CangussuAdalberto Pessoainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMGinstname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMGLICENSELicense.txtLicense.txttext/plain; charset=utf-82042https://repositorio.ufmg.br/bitstream/1843/42608/1/License.txtfa505098d172de0bc8864fc1287ffe22MD51ORIGINALTotal combining power technique for the evaluation of the quality control process of clostridiosis vaccines.pdfTotal combining power technique for the evaluation of the quality control process of clostridiosis vaccines.pdfapplication/pdf266635https://repositorio.ufmg.br/bitstream/1843/42608/2/Total%20combining%20power%20technique%20for%20the%20evaluation%20of%20the%20quality%20control%20process%20of%20clostridiosis%20vaccines.pdf46221fb70cdcc11dd933c01ce5f00143MD521843/426082022-06-23 08:14:05.132oai:repositorio.ufmg.br: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Repositório de PublicaçõesPUBhttps://repositorio.ufmg.br/oaiopendoar:2022-06-23T11:14:05Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)false |
| dc.title.pt_BR.fl_str_mv |
Total combining power: technique for the evaluation of the quality control process of clostridiosis vaccines |
| dc.title.alternative.pt_BR.fl_str_mv |
Poder total de combinação: Técnica para avaliação do processo de controle de qualidade de vacinas contra clostridiose |
| title |
Total combining power: technique for the evaluation of the quality control process of clostridiosis vaccines |
| spellingShingle |
Total combining power: technique for the evaluation of the quality control process of clostridiosis vaccines Igor Viana Brandi Vacina veterinária Clostridioses Toxinas bacterianas |
| title_short |
Total combining power: technique for the evaluation of the quality control process of clostridiosis vaccines |
| title_full |
Total combining power: technique for the evaluation of the quality control process of clostridiosis vaccines |
| title_fullStr |
Total combining power: technique for the evaluation of the quality control process of clostridiosis vaccines |
| title_full_unstemmed |
Total combining power: technique for the evaluation of the quality control process of clostridiosis vaccines |
| title_sort |
Total combining power: technique for the evaluation of the quality control process of clostridiosis vaccines |
| author |
Igor Viana Brandi |
| author_facet |
Igor Viana Brandi Eliane Macedo Sobrinho Santos Bruna Mara Aparecida de Carvalho Mesquita Carla Adriana Ferreira Durães Paula Karoline Soares Farias Roger Shoji Sari Alex Sander Rodrigues Cangussu Adalberto Pessoa |
| author_role |
author |
| author2 |
Eliane Macedo Sobrinho Santos Bruna Mara Aparecida de Carvalho Mesquita Carla Adriana Ferreira Durães Paula Karoline Soares Farias Roger Shoji Sari Alex Sander Rodrigues Cangussu Adalberto Pessoa |
| author2_role |
author author author author author author author |
| dc.contributor.author.fl_str_mv |
Igor Viana Brandi Eliane Macedo Sobrinho Santos Bruna Mara Aparecida de Carvalho Mesquita Carla Adriana Ferreira Durães Paula Karoline Soares Farias Roger Shoji Sari Alex Sander Rodrigues Cangussu Adalberto Pessoa |
| dc.subject.other.pt_BR.fl_str_mv |
Vacina veterinária Clostridioses Toxinas bacterianas |
| topic |
Vacina veterinária Clostridioses Toxinas bacterianas |
| description |
Outra Agência |
| publishDate |
2016 |
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2016 |
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2022-06-23T11:14:04Z |
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2022-06-23T11:14:04Z |
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info:eu-repo/semantics/publishedVersion |
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http://hdl.handle.net/1843/42608 |
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https://doi.org/10.1016/j.mimet.2016.08.023 |
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0167-7012 |
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https://orcid.org/0000-0001-6714-7996 https://orcid.org/ 0000-0001-8980-8599 |
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eng |
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Journal of Microbiological Methods |
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Universidade Federal de Minas Gerais |
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UFMG |
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Brasil |
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ICA - INSTITUTO DE CIÊNCIAS AGRÁRIAS |
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Universidade Federal de Minas Gerais |
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