Anti-mitochondrial tryparedoxin peroxidase monoclonal antibody-based immunohistochemistry for diagnosis of cutaneous leishmaniasis

Detalhes bibliográficos
Autor(a) principal: Mariana Lourenço Freire
Data de Publicação: 2022
Outros Autores: Felipe Dutra Rego, Karine Ferreira Lopes, Lucélia Antunes Coutinho, Rafaella Fortini Queiroz Grenfell, Daniel Moreira Avelar, Glaucia Fernandes Cota, Marcelo Antônio Pascoal-Xavier, Edward Oliveira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFMG
Texto Completo: https://doi.org/10.3389/fmicb.2021.790906
http://hdl.handle.net/1843/61858
https://orcid.org/0000-0003-0538-7403
Resumo: CNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
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spelling 2023-12-07T21:39:57Z2023-12-07T21:39:57Z2022-02-2812114https://doi.org/10.3389/fmicb.2021.7909061664-302Xhttp://hdl.handle.net/1843/61858https://orcid.org/0000-0003-0538-7403CNPq - Conselho Nacional de Desenvolvimento Científico e TecnológicoCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível SuperiorOutra AgênciaCutaneous leishmaniasis (CL) remains a globally spreading public health problem. Among Latin America countries, Brazil has the greatest number of recorded CL cases with several Leishmania species being associated with human cases. Laboratory diagnosis is one of the major challenges to disease control due to the low accuracy of parasitological techniques, the restricted use of molecular techniques, and the importance of differential diagnosis with regard to several dermatological and systemic diseases. In response, we have developed and validated an immunohistochemistry (IHC) technique for CL diagnosis using anti-mTXNPx monoclonal antibody (mAb). Recombinant Leishmania–mTXNPx was produced and used as an immunogen for mAb production through the somatic hybridization technique. The viability of mAb labeling of Leishmania amastigotes was tested by IHC performed with skin biopsies from hamsters experimentally infected with Leishmania amazonensis, Leishmania braziliensis, and Leishmania guyanensis. The enzymes horseradish peroxidase (IHC-HRP) and alkaline phosphatase (IHC-AP), both biotin-free polymer detection systems, were used in the standardization step. The IHC was further validated with skin biopsies from 49 CL patients diagnosed by clinical examination and quantitative real-time polymerase chain reaction and from 37 patients presenting other dermatological infectious diseases. Other parasitological techniques, such as direct examination and culture, were also performed for confirmed CL patients. Histopathology and IHC were performed for all included patients. Overall, the highest sensitivity was observed for IHC-AP (85.7%), followed by IHC-HRP (79.6%), direct examination (77.6%), histopathological examination (HE; 65.3%), and in vitro culture (49%). Only IHC and HE presented specificity over 90% and were able to detect CL patients regardless of parasite burden (odds ratio > 1.94; 95%CI: 0.34–11.23). A significant increase in positivity rates was observed when IHC-AP was combined with direct examination (95.9%) and HE (93.9%). The IHC techniques evaluated in here detected the main Leishmania species causing CL in Brazil and can support diagnostic strategies for controlling this neglected disease, especially if used in combination with other approaches for an integrative laboratorial diagnosis.engUniversidade Federal de Minas GeraisUFMGBrasilHCL - HOSPITAL DAS CLINICASMED - DEPARTAMENTO DE ANATOMIA PATOLÓGICA E MEDICINA LEGALFrontiers in MicrobiologyLeishmaniose cutâneaImuno-HistoquímicaAnticorpos MonoclonaisTécnicas de Laboratório ClínicoCutaneous leishmaniasisImmunohistochemistryDiagnosisMonoclonal antibodymTXNPxAnti-mitochondrial tryparedoxin peroxidase monoclonal antibody-based immunohistochemistry for diagnosis of cutaneous leishmaniasisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttps://www.frontiersin.org/articles/10.3389/fmicb.2021.790906/fullMariana Lourenço FreireFelipe Dutra RegoKarine Ferreira LopesLucélia Antunes CoutinhoRafaella Fortini Queiroz GrenfellDaniel Moreira AvelarGlaucia Fernandes CotaMarcelo Antônio Pascoal-XavierEdward Oliveiraapplication/pdfinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMGinstname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMGLICENSELicense.txtLicense.txttext/plain; charset=utf-82042https://repositorio.ufmg.br/bitstream/1843/61858/1/License.txtfa505098d172de0bc8864fc1287ffe22MD51ORIGINALAnti-mitochondrial tryparedoxin peroxidase monoclonal antibody-based immunohistochemistry for diagnosis of cutaneous leishmaniasis.pdfAnti-mitochondrial tryparedoxin peroxidase monoclonal antibody-based immunohistochemistry for diagnosis of cutaneous leishmaniasis.pdfapplication/pdf3936456https://repositorio.ufmg.br/bitstream/1843/61858/2/Anti-mitochondrial%20tryparedoxin%20peroxidase%20monoclonal%20antibody-based%20immunohistochemistry%20for%20diagnosis%20of%20cutaneous%20leishmaniasis.pdf5af5297a65dbc8c6218d4df4d5e3d0e1MD521843/618582023-12-07 18:39:57.454oai:repositorio.ufmg.br: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Repositório de PublicaçõesPUBhttps://repositorio.ufmg.br/oaiopendoar:2023-12-07T21:39:57Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)false
dc.title.pt_BR.fl_str_mv Anti-mitochondrial tryparedoxin peroxidase monoclonal antibody-based immunohistochemistry for diagnosis of cutaneous leishmaniasis
title Anti-mitochondrial tryparedoxin peroxidase monoclonal antibody-based immunohistochemistry for diagnosis of cutaneous leishmaniasis
spellingShingle Anti-mitochondrial tryparedoxin peroxidase monoclonal antibody-based immunohistochemistry for diagnosis of cutaneous leishmaniasis
Mariana Lourenço Freire
Cutaneous leishmaniasis
Immunohistochemistry
Diagnosis
Monoclonal antibody
mTXNPx
Leishmaniose cutânea
Imuno-Histoquímica
Anticorpos Monoclonais
Técnicas de Laboratório Clínico
title_short Anti-mitochondrial tryparedoxin peroxidase monoclonal antibody-based immunohistochemistry for diagnosis of cutaneous leishmaniasis
title_full Anti-mitochondrial tryparedoxin peroxidase monoclonal antibody-based immunohistochemistry for diagnosis of cutaneous leishmaniasis
title_fullStr Anti-mitochondrial tryparedoxin peroxidase monoclonal antibody-based immunohistochemistry for diagnosis of cutaneous leishmaniasis
title_full_unstemmed Anti-mitochondrial tryparedoxin peroxidase monoclonal antibody-based immunohistochemistry for diagnosis of cutaneous leishmaniasis
title_sort Anti-mitochondrial tryparedoxin peroxidase monoclonal antibody-based immunohistochemistry for diagnosis of cutaneous leishmaniasis
author Mariana Lourenço Freire
author_facet Mariana Lourenço Freire
Felipe Dutra Rego
Karine Ferreira Lopes
Lucélia Antunes Coutinho
Rafaella Fortini Queiroz Grenfell
Daniel Moreira Avelar
Glaucia Fernandes Cota
Marcelo Antônio Pascoal-Xavier
Edward Oliveira
author_role author
author2 Felipe Dutra Rego
Karine Ferreira Lopes
Lucélia Antunes Coutinho
Rafaella Fortini Queiroz Grenfell
Daniel Moreira Avelar
Glaucia Fernandes Cota
Marcelo Antônio Pascoal-Xavier
Edward Oliveira
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Mariana Lourenço Freire
Felipe Dutra Rego
Karine Ferreira Lopes
Lucélia Antunes Coutinho
Rafaella Fortini Queiroz Grenfell
Daniel Moreira Avelar
Glaucia Fernandes Cota
Marcelo Antônio Pascoal-Xavier
Edward Oliveira
dc.subject.por.fl_str_mv Cutaneous leishmaniasis
Immunohistochemistry
Diagnosis
Monoclonal antibody
mTXNPx
topic Cutaneous leishmaniasis
Immunohistochemistry
Diagnosis
Monoclonal antibody
mTXNPx
Leishmaniose cutânea
Imuno-Histoquímica
Anticorpos Monoclonais
Técnicas de Laboratório Clínico
dc.subject.other.pt_BR.fl_str_mv Leishmaniose cutânea
Imuno-Histoquímica
Anticorpos Monoclonais
Técnicas de Laboratório Clínico
description CNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
publishDate 2022
dc.date.issued.fl_str_mv 2022-02-28
dc.date.accessioned.fl_str_mv 2023-12-07T21:39:57Z
dc.date.available.fl_str_mv 2023-12-07T21:39:57Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1843/61858
dc.identifier.doi.pt_BR.fl_str_mv https://doi.org/10.3389/fmicb.2021.790906
dc.identifier.issn.pt_BR.fl_str_mv 1664-302X
dc.identifier.orcid.pt_BR.fl_str_mv https://orcid.org/0000-0003-0538-7403
url https://doi.org/10.3389/fmicb.2021.790906
http://hdl.handle.net/1843/61858
https://orcid.org/0000-0003-0538-7403
identifier_str_mv 1664-302X
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartof.pt_BR.fl_str_mv Frontiers in Microbiology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Minas Gerais
dc.publisher.initials.fl_str_mv UFMG
dc.publisher.country.fl_str_mv Brasil
dc.publisher.department.fl_str_mv HCL - HOSPITAL DAS CLINICAS
MED - DEPARTAMENTO DE ANATOMIA PATOLÓGICA E MEDICINA LEGAL
publisher.none.fl_str_mv Universidade Federal de Minas Gerais
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFMG
instname:Universidade Federal de Minas Gerais (UFMG)
instacron:UFMG
instname_str Universidade Federal de Minas Gerais (UFMG)
instacron_str UFMG
institution UFMG
reponame_str Repositório Institucional da UFMG
collection Repositório Institucional da UFMG
bitstream.url.fl_str_mv https://repositorio.ufmg.br/bitstream/1843/61858/1/License.txt
https://repositorio.ufmg.br/bitstream/1843/61858/2/Anti-mitochondrial%20tryparedoxin%20peroxidase%20monoclonal%20antibody-based%20immunohistochemistry%20for%20diagnosis%20of%20cutaneous%20leishmaniasis.pdf
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